FTIR Microscopy and Imaging - Agilent FTIR Microscopy and Imaging A world of applications and...

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Transcript of FTIR Microscopy and Imaging - Agilent FTIR Microscopy and Imaging A world of applications and...

  • FTIR Microscopy and Imaging

    A world of applications and solutions

    Dr. Mustafa Kansiz

    FTIR Imaging and Microscopy Product Manager

    Agilent Technologies

    Email: mustafa.kansiz@agilent.com

  • FTIR Spectroscopy - What is it?

    • Fourier Transform InfraRed Spectroscopy is the study of the

    interaction of infrared light with matter.

    • The vibrations of bonds between atoms in a molecule are

    excited by IR light leading to absorbances that are specific

    to chemical structure specific

  • How can FTIR microscopy imaging help me?

    An FTIR microscope has two essential purposes:

    1. To allow users to visually see small (micron) sized samples

    2. Collect accurate FTIR spectra from small samples

    FTIR Imaging takes this to another level by providing spatial

    and spectral information from an area on your sample

    FTIR microscopy can collect data in four modes:

    1. Single point

    2. Single point mapping

    3. Linear array mapping

    4. 2-D Focal Plane Array (FPA) imaging

    FTIR Imaging gives spatial (WHERE) and spectral (WHAT) information, and FTIR

    Chemical Imaging with a IR Focal Plane Array (FPA), gives this simultaneously

  • FTIR Microscope Measurement Modes

    1 : Single Point

    Single or multiple spectra of different zones of a sample

    2: Single Point Mapping Automated acquisition of spectra

    (one by one) defined by a grid. A

    hundred points can take several

    hours.

  • FTIR Microscope Measurement Modes:

    4: FPA Imaging

    With an FPA detector, up to 16384 spectra can be recorded

    simultaneously in a single

    measurement.

    3: Linear array Mapping Acquisition of spectra by a row

    (1x16) of detectors. Faster than

    single point mapping, but still

    much slower than FPA imaging

    Why use FPA chemical imaging?

    Two reasons:

    1. Provides rapid high spatial resolution chemical distribution – the where (spatial) and the what (spectral)

    2. Allows for the measurement of defects as small as a ~2 microns

  • What do the Coloured Images Mean?

    3500 3000 2500 2000 1500 1000

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    ATR Chemical Image

    Image @

    1724 cm-1

    PET 7

    0 u

    m

    70 um

    C

    O

    =

    O

  • What do the Coloured Images Mean?

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    b s o rb

    a n c e

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    u m

    70 um

    ATR Chemical Image

    Image @

    1724 cm-1

    PET

    Image @

    3295 cm-1

    Nylon

    N

    H

    -

    C

    =

    O

    O

  • What do the Coloured Images Mean?

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    b s o rb

    a n c e

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    0.15

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    Wavenumber

    A b s o rb

    a n c e

    ATR Chemical Image

    Image @

    1724 cm-1

    PET

    Image @

    3295 cm-1

    Nylon

    Image @

    2915cm-1

    PP

    7 0

    u m

    70 um

    C

    O

    =

    O

    N

    H

    -

    C

    H

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  • FTIR Chemical Imaging and its applications Materials/Polymer Polymer laminates (functional

    layer & adhesive identification)

    Defect analysis

    Phase distribution

    Composites

    Biomedical/Biological Research

    Early disease diagnosis (Cancers)

    Study of diseases (Alzheimer’s, kidney)

    Plant/fungi tissue studies

    Live cell chemical imaging (in water)

    Microbial identification

    Bone, teeth and cartilage

    Forensics Car paint layer & structure analysis

    Trace evidence analysis

    Pharmaceuticals Ingredient distribution

    Coating analysis

    Defect/particle analysis

    Electronics Defect analysis

    Art Conservation Painting components & layer identification

    Geology Study of inclusions

    Food/Cosmetics Study of emulsions, eg cheese, mayonnaise,

    cream

  • Infrared Microscopy Sampling Techniques

    Transmission

    Sample thickness:

    10 – 20 mm

    Reflectance

    Sample thickness:

    NA

    Absorption/

    Reflectance

    Sample thickness:

    5 - 10 mm

    Objective

    Sample

    Condenser

    Stage

    θ θ θ

    Micro - ATR

    Sample thickness:

    NA

    “Kevley SlideTM”

    θ

    ATR - IRE

  • Large Sample Microscopy Sampling Techniques – Measure small areas on large samples

    Large Sample

    Reflectance

    Sample thickness:

    NA Sample thickness:

    NA

    Sample thickness:

    NA

    large

    sample

    External

    sample

    stage

    large

    sample

    large

    sample

    Large Sample

    Micro ATR

    Large Sample

    Grazing Angle

    O b je

    c ti v e

    O b je

    c ti v e

    G A

    O

    External

    sample

    stage

    External

    sample

    stage

  • Agilent’s patented1 Large Sample Microscope Objective allows you to measure unlimited sized samples in reflection or ATR single point or imaging mode

    It’s as simple as:

    1. Taking your sample to the microscope

    2. Placing it against the 90 degree objective

    3. Press collect and get your results!

    Agilent’s patented1 Large Sample Microscope Objective allows you to measure unlimited sized samples in reflection or ATR single point or imaging mode

    Examples:

    1. Investigation of new coating materials on helmets (military / aerospace / etc.)

    Agilent’s patented1 Large Sample Microscope Objective allows you to measure unlimited sized samples in reflection or ATR single point or imaging mode

    Examples:

    1. Investigation of new coating materials on helmets (military / aerospace / etc.)

    2. Large vehicle component analysis, ex: car door/panel

    Large sample

    15x Objective

    Mirror assembly

    3000 2000 1000

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    cm-1

    A b

    s .

    LS

    Large Sample Objective

    VARIAN/Marketing/Marcom/TVEII/Refined animtions/flexibility/flexibility.exe

  • Single point analysis (Single-element detector analysis)

  • Single Point Analysis

    1 detector element collects 1 spectrum per scan

    Desired spatial resolution is attained by fixing aperture size to desired sampling size. This

    eliminates spectral interference from the surrounding area.

    Typical best achievable spatial resolution is 10 - 20 µm.

  • Transmission IR Analysis of Microtomed 3 layer Laminate

    4000 3000 2000 1000

    0.8

    0.4

    0.0

    A b

    s o rb

    a n

    c e

    Wavenumber (cm-1)

    4000 3000 2000 1000

    0.8

    0.4

    0.0

    A b

    s o rb

    a n

    c e

    Wavenumber (cm-1)

    4000 3000 2000 1000

    0.8

    0.4

    0.0

    A b

    s o rb

    a n

    c e

    Wavenumber (cm-1)

  • Plastic Tubing – Defect analysis

    ATR

    ATR

    Tube spectrum #2

    Tube spectrum #3

    3500 3000 2500 2000 1500 1000

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    0.06

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    -0.02

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    Wavenumber

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    s o

    rb a

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    4x glass visible objective

    15x reflective vis/IR objective

    15x reflective vis/IR objective

    2.5 mm

    2 .0

    m m

    660 mm

    5 0

    0 m

    m

    660 mm

    5 0

    0 m

    m

  • 4x glass visible objective

    15x reflective vis/IR objective

    ATR

    Glue spectrum #1

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    0.06

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    Wavenumber

    A b s o rb

    a n c e

    2.5 mm

    2 .0

    m m

    660 mm

    5 0

    0 m

    m

    Plastic Tubing – Defect analysis

  • Infrared Mapping

    - Single Point

    - Linear Arrays

  • Infrared Single Point Mapping