Unconjugated Estriol RIA - · PDF fileISO 13485 Unconjugated Estriol RIA Code: RK-3CT 100...
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ISO 13485 U U n n c c o o n n j j u u g g a a t t e e d d E E s s t t r r i i o o l l R R I I A A Code: RK-3CT 100 tubes Radioimmunoassay for the quantitative in vitro determination of unconjugated Estriol (uE3) in human serum. ESSENTIALS • Simple format, coated tube assay • One step procedure, no washing THEORY IN PICTURES FLOW CHART Add to coated tubes: • 50 µL calibrator/control/sample • 500 µL tracer • 500 µL antiserum 2 h incubation at RT with shaking Competition between unlabelled uE3 and 125 I-labelled uE3 for a limited number of binding sites on uE3 specific antibody.
Transcript of Unconjugated Estriol RIA - · PDF fileISO 13485 Unconjugated Estriol RIA Code: RK-3CT 100...
ISO 13485
UUUnnncccooonnnjjjuuugggaaattteeeddd EEEssstttrrriiiooolll RRRIIIAAA
Code: RK-3CT 100 tubes
Radioimmunoassay for the quantitative in vitro
determination of unconjugated Estriol
(uE3) in human serum.
ESSENTIALS
• Simple format, coated tube assay
• One step procedure, no washing
THEORY IN PICTURES
FLOW CHART
Add to coated tubes:
• 50 µL
calibrator/control/sample
• 500 µL tracer
• 500 µL antiserum
2 h incubation at RT with shaking
Competition between unlabelled uE3 and 125I-labelled uE3 for a
limited number of binding sites on uE3 specific antibody.
ISO 13485
UUUnnncccooonnnjjjuuugggaaattteeeddd EEEssstttrrriiiooolll RRRIIIAAA
PERFORMANCE PARAMETERS
Sample volume: 50 µL
Standard range: 0 – 120 nmol/L
Analytical sensitivity: 0.4 nmol/L
Cross-reactivity with Estriol sulfate and glucoronide ≤ 3%
Expected range in the second trimester of pregnancy:
Weeks n Median (nmol/L)
14 22 6.4
15 1107 7.7
16 1019 9.3
17 137 11.2
18 62 12.9
METHOD COMPARISON
INSTITUTE OF ISOTOPES Co. Ltd.
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Tel.: +36-392-2577, Fax: +36-395-9247
Website: http://www.izotop.hu
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