ISO 13485

UUUnnncccooonnnjjjuuugggaaattteeeddd EEEssstttrrriiiooolll RRRIIIAAA

Code: RK-3CT 100 tubes

Radioimmunoassay for the quantitative in vitro

determination of unconjugated Estriol

(uE3) in human serum.

ESSENTIALS

• Simple format, coated tube assay

• One step procedure, no washing

THEORY IN PICTURES

FLOW CHART

Add to coated tubes:

• 50 µL

calibrator/control/sample

• 500 µL tracer

• 500 µL antiserum

2 h incubation at RT with shaking

Competition between unlabelled uE3 and 125I-labelled uE3 for a

limited number of binding sites on uE3 specific antibody.

ISO 13485

UUUnnncccooonnnjjjuuugggaaattteeeddd EEEssstttrrriiiooolll RRRIIIAAA

PERFORMANCE PARAMETERS

Sample volume: 50 µL

Standard range: 0 – 120 nmol/L

Analytical sensitivity: 0.4 nmol/L

Cross-reactivity with Estriol sulfate and glucoronide ≤ 3%

Expected range in the second trimester of pregnancy:

Weeks n Median (nmol/L)

14 22 6.4

15 1107 7.7

16 1019 9.3

17 137 11.2

18 62 12.9

METHOD COMPARISON

INSTITUTE OF ISOTOPES Co. Ltd.

1535 Budapest. POB.: 851.

Tel.: +36-392-2577, Fax: +36-395-9247

Website: http://www.izotop.hu

Technical e-mail: immuno@izotop.hu

Commercial e-mail: commerce@izotop.hu