Jornada de Actualización en Biología de la Reproducción

Congreso ESHRE 2016

PGS y ADN Mitocondrial

Sesión 16

Claudio Bisioli

16. PGS

• Day 7 blasts. prove beneficial for PGS cycles.

• John Whitney, USA

La necesidad de ser cautos

• Tienen 3 nacidos con transfer de blastos en D7 y ninguna anormalidad observada

• El cultivo prolongado pude exponer a los embriones a factores epigenéticos y/o efectos desconocidos a largo plazo sobre el desarrollo

• Deben ser considerados en última instancia para transferirlos

16. PGS

• Evidence that differences between embryology laboratories can influence the rate of mitotic errors, leading to increased chromosomal mosaicism, with significant implications for IVF success rates.

• Dagan Wells, UK

P-203 Euploid rate sensitivity to laboratory culture environment: a blind, prospective, randomised, sibling study C. Hickman1 , D. Wells2 , et al.

The Fertility Partnership, Boston Place Clinic, London, UK 2 Reprogenetics UK, Oxford, UK 3 The Fertility Partnership, IVF Hammersmith, London, UK

• Study question: To determine whether culturing embryos in two different single step culture media affects the proportion of chromosomally normal embryos

• Medium 1 (KSOM supplemented with complex protein) was compared with Medium 2 (Sage supplemented with Human Serum Albumin, HSA)

• the proportion of chromosomally normal embryos was almost two-fold greater with treatment 2 (16%, N = 141 vs. 29%, N = 154, p < 0.025).

• embryos cultured in medium 2 reached the blastocyst stage faster

• This study suggests that ploidy rates and morphokinetic parameters are sensitive to environmental change, and, therefore, should be monitored as part of the quality control programme in the IVF laboratory.

Jornada de Actualización en Biología de la Reproducción

Congreso ESHRE 2016

PGS y ADN Mitocondrial

Sesión 16

Claudio Bisioli

Hipótesis del Estrés Energético debido a un descenso en el “combustible metabólico” El embrión compensa esa falta produci mayor número de mitocondrias

16. mitochondrial DNA

• Quantification of mitochondrial DNA in preimplantation embryos: a tool to predict implantation potential of chromosomally normal embryos

• Francesca Spinella, Italia

16. mitochondrial DNA

• Clinical implications of mitochondrial DNA quantification on pregnancy outcomes: a blinded prospective non-selection study.

• Elpida Fragouli, UK

Session 1: Opening

2 Keynote lectures

• Allan Pacey: Paper with the highest number of full-text

downloads during their first six months of publication in the

journal between January 2014 and June 2015

• Johan Eriksson: Helsinki Birth Control Study

Session 1: Keynote Session Modifiable and non-modifiable risk factors for poor sperm morphology

Allan Pacey

• CHAPS-UK

• Estudio sobre variables seminales

a lo largo del tiempo en 14 clínicas de FIV de UK.

1. Trabajo con solventes orgánicos → disminuye el conteo espermático

• No pudieron identificar ningún otro riesgo ocupacional.

• Cherry et al. 2008. Occup Environm Med 65:708-714

Chemicals and Pregnancy Study-UK (CHAPS-UK): Multicentre Study of Occupational Exposure to Chemicals and Male Fertility

Session 1: Keynote Session Modifiable and non-modifiable risk factors for poor sperm morphology

Allan Pacey

2. Povey 2012 HR 27:2799-2806

• “Modificable”: BMI, ocupación, ropa interior, alcohol, tabaco, drogas, etc.

• “No modificable”: edad, etnicidad, enfermedades previas, etc.

Session 1: Keynote Session Modifiable and non-modifiable risk factors for poor sperm morphology

Allan Pacey

3. Pacey 2014 HR 29:1629-1636 Modifiable and non-modifiable risk factors for poor sperm morphology

• CHAPS-UK → Morfología

• Encuentran pocas variables asociadas: – Estacionalidad

– Cannabis

– Abstinencia

• “We feel there are few things that we can do to change their sperm quality”

Session 1: Keynote Session Long term consequences of maternal obesity on the health of offspring

Johan Eriksson, Finlandia

Helsinki Birth Control Study

• Human embryos from overweight and obese women display phenotypic and metabolic abnormalities. Leavy Christine, Leese Henry, Sturney Roger. Hum Reprod 2015, 30:122-132

• Obesidad tiene efectos a largo plazo sobre la salud del bebé.

• Eriksson JG Am Med 2016: “programming” y la “Barker Hypothesis”: the period of gestation has significant impacts on the developmental health and wellbeing outcomes for an individual ranging from infancy to adulthood

• Una fuerte relación/asociación entre la adiposidad materna y la adiposidad de la descendencia

• En el hijo:

– Enfermedad cardio vascular

– CHD: coronary heart disease

– Infarto

– Diabetes

• Fuertemente asociadas a obesidad materna

Sesiones de la mañana • 26. Do IVF patients deserve standard treatments? • 27. Epigenetic remodelling in embryos • 28. ASRM exchange session. KF (Rebecca Sokol) y Turner syndromes

(Richard Reindollar) • 29. Nursing • 30. IVF laboratory quality and strategies • 31. Live surgery session • 32. Endometrial receptivity • 33. Deep sequencing the embryo • 34. Reproductive epidemiology, socio-cultural aspects and health

economy • 35. AMH • 36. European and global ART monitoring session

– Europa – ICMART World Report 2012 – ICMART in Africa

• 37. Update on ultrasound imaging • 38. Reproductive endocrinology / Early pregnancy • 39. Nursing

Magnetic resonance imaging (MRI))

Sesiones de la tarde • 40. The need for oxygen in IVF • 41. Insulin sensitizers in reproduction • 42. Embryo and double gamete donation (patient session) • 43. Legislation and economics • 44. Nursing • 45. Epigenetics and miRNAs • 46. The uterus in infertility • 47. Trophoblast and endometrial cross talk • 48. Safety and quality in ART 1 • 49. Stem cells • 50. Effects of age and health on semen parameters and sperm DNA

integrity • 51. Oocyte and sperm quality • 52. When sperm are the limit • 53. Genetic factors in infertility • 54. Reproductive surgery • 55. Female fertility: New and renewed ideas. • 56. Ethics and law