Summer PDSI Research

Summer 2009Michael Plater II

WHAT:

WHAT:

WHAT:Anthocyanin

BENEFITS:

Antioxidant

Natural Food

Coloring

WHY:

Antioxidant Improving Human Health

Natural Food Coloring Increasing Economic Rates of Return

Total Rice Value

HOW:

Rough

Rice

Husks

Shelled

Rice

Milled

RiceBran

HOW:

McGill Rice Mill

Fractionization Three milling times

Determine

concentrations at

different fractions

HOW: Extraction

BRAN

Anthocyanin

Milling (3 Diff

Times)Hexane

Dry

Ethanol

HPLC

Thank You:Questions?

Rough Rice, Milled Rice, Bran

Methods:

Using a McGill rice millGenerate triplicate samples of bran

layer at 10, 25, 40 seconds Extract using 5ml of hexane with

0.5g of bran Filter and dry bran Extract using ethanol with 5ml Run through High Pressure Liquid

Chromatography

Timeline:

- 10 Sec

Hexane

- 25 Sec

Hexane

- 10 Sec

Ethanol

- 40 Sec

Hexane

- 25 Sec

Ethanol

- 40 Sec

Ethanol

- HPLC for all

fractions