Department of Proteomic and Microbiology - UMons - Lab'InSight Proteomics

Department of Proteomic and Microbiology - UMons - Lab'InSight Proteomics
Department of Proteomic and Microbiology - UMons - Lab'InSight Proteomics
Department of Proteomic and Microbiology - UMons - Lab'InSight Proteomics
Department of Proteomic and Microbiology - UMons - Lab'InSight Proteomics
Department of Proteomic and Microbiology - UMons - Lab'InSight Proteomics
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description of the laboratory skills (09/03/2010)

Transcript of Department of Proteomic and Microbiology - UMons - Lab'InSight Proteomics

  • 1. 18/03/10 1 DepartmentofProteomicandMicrobiology (ProtMic) UniversityofMons 2 UniversityofLige9March2010 Proteinsexpression/ purica>on Valida>on Protein characteriza>on Proteome Prefrac>ona>on Proteomicstrategy op>miza>on ProjectconceptatProtMic Proteinstarget discovery Experimentaldesign Globalanalysisvs.Targetedanalysis Gelbasedapproachvs.gelfree Labelfreevs.Labelbasedquan>ca>on Prefrac>ona>onneeded?

2. 18/03/10 2 3 UniversityofLige9March2010 Proteinsexpression/ purica>on Valida>on Protein characteriza>on Proteome Prefrac>ona>on Proteomicstrategy op>miza>on ProjectconceptatProtMic Proteinstarget discovery Targetedanalyses Globalanalyses TiO2phopshopep=desenrichment Surfaceexposedproteinspurica=on TopdownorboLomupapproaches IMACbasedproteomeprefrac=ona=on HPLCandFPLC Liquidisoelectrofocusing(proteinorpep=deslevel) NewDPCchips 4 UniversityofLige9March2010 Proteinsexpression/ purica>on Valida>on Protein characteriza>on Proteome Prefrac>ona>on Proteomicstrategy op>miza>on ProjectconceptatProtMic Proteinstarget discovery Gelbasedproteomic(Immunoproteomic) Gelfreedieren>alproteomic 3. 18/03/10 3 5 UniversityofLige9March2010 Proteinsexpression/ purica>on Valida>on Protein characteriza>on Proteome Prefrac>ona>on Proteomicstrategy op>miza>on ProjectconceptatProtMic Proteinstarget discovery Bacterial expression Tag-based purification Untagged proteins purification 6 UniversityofLige9March2010 Proteinsexpression/ purica>on Valida>on Protein characteriza>on Proteome Prefrac>ona>on Proteomicstrategy op>miza>on ProjectconceptatProtMic Proteinstarget discovery An=bodybasedvalida=on MSbasedvalida=on 4. 18/03/10 4 UniversityofLige9March2010 Proteinsexpression/ purica>on Valida>on Protein characteriza>on Proteome Prefrac>ona>on Proteomicstrategy op>miza>on ProjectconceptatProtMic Proteinstarget discovery Enzymeac>vity Structuralcharacteriza>on MSbasedcharacteriza>on Aminoacidanalysis Chromatographyanalysis(HPLC,) Spectrophotometrycharacteriza>on Purity Posttransla-onalmodica-on studies(Phosphoryla-on,acetyla-on, formyla-on,determina-onofSSlink.) Proteinmoleculeinterac-ons Conforma-onalstudies UniversityofLige9March2010 ProjectconceptatProtMic Interdisciplinarycenterofmassspectrometry:CISMA Anewacademictooldedicatedtosmallmoleculesand synthe>corbiologicalpolymers opentotheacademicandindustrialpartners Scien=cboarding:Dr.P.Gerbaux Prof.R.Muller Prof.P.Dubois Prof.M.DeMeyer Prof.R.Sneyders Director:Prof.R.WaYez Ruddy.waYez@umons.ac.be 10complementaryMS instruments Highexperiencelevelin smallandlargemolecules 5. 18/03/10 5 ThankyouforyouraLen>on