Como configurar una Calibración ChemStation

ChemStation E-Seminars

ChemStation E-Seminar

Dec, 2008

ChemStation E-Seminar Agenda Schedule Short description AgilentSchedule Short description Agilent

Speaker

15. Oct. 2008 ChemStation Data Storage Concept Steven Brown

Ortrud Emde

12. Nov. 2008 ChemStation Navigation TableHow to improve Review and Reprocess

Steven Brown

Ortrud EmdeOrtrud Emde10. Dec. 2008 Integrating My Results in ChemStation Steven Brown

Ortrud Emde

14. Jan. 2009 How to set up a calibration table in ChemStation Steven Brown

Bob Guiffre

04. Feb. 2009 Various Reporting in Chemstation Steven Brown

Ortrud Emde

18. Feb. 2009 Updated Compliance 21 CFR Part 11 solution with Steven Brown


Dec, 2008

18. Feb. 2009 Updated Compliance 21 CFR Part 11 solution with Agilent ChemStation & OpenLAB ECM

Steven Brown

Ortrud Emde

Seminar 4: How to set up a calibration Table in ChemStationChemStation

Topics

OpenLAB Overview

Calibration and peak Identification Overview

Demo Simple Calibration table

C lib ti S ttiCalibration Setting

Multilevel calibration

Recalibration Sequence and Manual

Questions


Dec, 2008

Q tifi ti C lib tiQuantification - Calibration

In this Section, We Will Discuss:

The steps necessary to build a calibration table.

How to select calibration settings.

Calibration table options.

Peak identification tools.

5

Building a Calibration Table

1. Inject standards with concentrations that bracket yourunknowns.

2. Set-up appropriate integration events for the low standard.Integrate to obtain the peak height or area. Save the integration events to the method.

3 Begin a new calibration table with level one the lowest3. Begin a new calibration table with level one, the lowestlevel. Fill in compound names and amounts.

4 I t t h dditi l t d d l l d dd t th4. Integrate each additional standard level and add to the calibration table.

6

5. Examine the calibration curve and save it to the method.

Example Calibration

mAU100

DAD1 A, Sig=254,4 Ref=550,100 (DEMO\005-0101.D)

0.7

47

021

40

60

80 1.0

.565

7

StandardISTD

min1 2 3 4 5 6

0

20

2

5.8

37

ng/μL ng/μL

3 Levels

1. Standard 30.0 ISTD 50.02. Standard 50.0 ISTD 50.03 Standard 75 0 ISTD 50 0

7

3. Standard 75.0 ISTD 50.0

Signal Details

Define signals to be evaluated during a method

8

Calibration Settings

9

Load the Signal(s) for the Low Level Standard and IntegrateIntegrate

10

Create a Calibration Table: Level One

11

Fill in:

Calibration Table Overview: Fill in the DialogFill in:Compound NameAmount

Check if:Reference PeakISTDIndicate ISTD #

12

Demo CalibrationDemo Calibration


Dec, 2008

Load the Signal - Add Level Two

Fill in Amount for Level 2

14

Fill in Amount for Level 2

Add Additional Levels

Add Level 3Add Level 3 and fill in theamount

15

Calibration Table Options

Compound, Amt, Area, Rsp Factor,Ref, ISTD#

Compound, Grp, Amt, Low LimitHigh Limit

Compound, Amt, Area, Def, CurveType, Origin, Weight

Compound, Area, Rsp%, Def, +-, Pk Usage

16

Compound Details

Use Low and High Limits as qualifiers to confirm peakidentification.

17

Peak Details

Check Def to apply thedefault curve type and origin from the Calibration Settingsdi l bdialog box.

Curve TypesPiece wise Exponent

Origin TreatmentsIgnore

Linear QuadraticLog CubicPower Average Rsp/Amt

IncludeForceConnect straight segment

18

Power Average Rsp/Amt Connect straight segment

Identification Details

By default the largest peak of a compound is the Main peakBy default, the largest peak of a compound is the Main peak. Others are set to Ignore.

19

Qualifiers

The ratio of 230 to 254 is 38% The ratio of 230 to 254 is 224%The ratio of 230 to 254 is 38%for Biphenyl

The ratio of 230 to 254 is 224%.The peak would be rejected

20

Edit Calibration Table Options

Define the columns included in your Calibration Table

21

Quantification Tools

C lib i T k

New Calibration Table

Add New Level Edi C lib iCalibration TaskTool

Add New Level

Signal DetailsAdd New Peaks

Edit CalibrationTable Options

Recalibrate withCurrent Chromatogram

Select compoundsfrom chromatogramg

Select Calibrated peak(s) fromChromatogram and delete from

lib ti t blSelect peak(s) from h t d dd

from chromatogram and recalibrate

22

calibration table. chromatogram and addto calibration table

To Save the Calibration Table as Part of a Method...

Save Table to Method

23

Save Method

• Be sure to know which method you are ki ith d h t th tworking with and save changes to the correct

method.

M th d D l t h (i lib ti• Method Development changes (i.e. calibration table creation / updates) should be saved to the Current Method (Master Method).

• Integration events and other parameters specific to the individual data file should be

d t th I di id l M th d f D t Filsaved to the Individual Method from Data File (DA.M)

24

Information on ChemStationDocumentation can be found on the Agilent websiteghttp://www.chem.agilent.com/Scripts/PDS.asp?lPage=61233

• Product Datasheet• Specification• Application Notes• Manuals

Manuals can be found your ChemStation Installation DVD

• Getting Started with New ChemStation WorkflowPartNo.G2170-90042

U d t di Ch St ti• Understanding your ChemStationPartNo. G2070-91125

• OpenLAB OptionPartNo.G2170-90233

Software Status Bulletinhttp://www.chem.agilent.com/en-US/Support/Downloads/Patches/Pages/default.aspx

Customer Trainings (NorthAmerica)g ( )

http://www.chem.agilent.com/cag/training/northam/index.asp


Dec, 2008March, 2008

Learning Products – North America Course CatalogCatalog

Users needing to increase productivity in the lab by utilizing both standard and advanced features available in the Agilent GC/LC ChemStation may want to attend one of the following courses:

H2606A - ChemStation for GC Data Analysis and Reporting (2 days)

H5928A - Agilent HPLC (2D) Data Analysis and Reporting (2 days)


Course Features

Data acquisition and method creationData analysis including integration and calibrationSequencingReportingReporting

For more information concerning course content, dates and locations, please visit:

http://www chem agilent com/en-US/education/en-us/classroomtraining/Pages/Courselisting aspx


Dec, 2008

http://www.chem.agilent.com/en US/education/en us/classroomtraining/Pages/Courselisting.aspx

Learning Products – European Course Catalog

Users needing to increase productivity in the lab by utilizing both standard and advanced features available in the Agilent GC/LC ChemStation may want to attend one of the following courses:

H4033A – Agilent HPLC (3D) Method&Run Control, Data Analysis and Reporting (4 days)


H5928A – Advanced User Training, Quantification and Result Reporting (2 days)

Course Features

Data acquisition and method creationData analysis including integration and calibrationSequencingReportingReporting

For more information concerning course content, dates and locations, please visit:

htt // h il t / US/ d ti / /h


Dec, 2008

http://www.chem.agilent.com/en-US/education/pages/homepage.aspx

Who to contact for more information about OpenLAB and Agilent software productsOpenLAB and Agilent software products1. Agilent Account Managers (sales)

2 Agilent Informatics Product Specialists2. Agilent Informatics Product Specialists

Eastern US - Marc Mandelbaum – 302-683-7149

Pacific Coast (CA, WA, OR) - Spencer Tse 408-553-3655

Midwest and South - Johnathon McSayles 847-944-6019

Midwest /West/Latin America – Stephen Brown 847-944-6461

QUESTIONS?


Dec, 2008

Como configurar una Calibración ChemStation

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