XE-5000™

Automated Hematology System

Your Complete Choice

Advanced Technology SolutionstoMeet Your Lab’s Needs

Today’s LaboratoryChallenges

Laboratories have continued to face a number ofchallenges for several years. These include clinical,operational and financial issues such as:

• Demand for clinically relevant information• Medical technologist labor shortage• Increased workload• Need for faster turnaround time• Requirement for high reliability• Limited laboratory budgets

The Sysmex XE-5000 Automated Hematology Systemutilizes the power of fluorescent flow cytometry andhydrodynamic focusing technologies. Using a unique,state-of-the-art, diode laser bench, Sysmex fluorescentflow cytometry provides the sensitivity needed formeasuring and differentiating cell types in whole bloodand body fluid samples. Fluorescent technology andhydrodynamic focusing enable the XE-5000 toconsistently classify normal WBC, RBC and PLTpopulations from abnormal populations, therebydecreasing the number of manual interventions.

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Even with these challenges, the need for hematologytesting has remained steady or continued to grow.Laboratories are searching for hematology analyzersthat can improve productivity and efficiency whileproviding enhanced clinical information. Designed tobe scalable, reliable and efficient, the Sysmex XE-5000™offers medium to high volume labs an automatedhematology system that can truly meet and exceedtheir expectations. The XE-5000 streamlines yourworkflow by providing rapid testing for up to 150samples per hour, enabling quick turnaround time.

Clinically Relevant Information

Fluorescent NucleatedRed Blood Cells

Enumeration of NRBC is critical toproviding an accurate WBC anddifferential in pathological samples.The XE-5000 fluorescent technologyprovides a NRBC in both % and # froma dedicated channel.

The NRBCmeasurement provides:

• Improved low-end sensitivity• Rapid reporting to assist clinicians

in patient diagnosis and treatmentdecisions

• Efficient automatic correction ofWBC and Diff results

• Decreased false negative andfalse positive rates with advancedseparation of WBCs and NRBCs

• Available with every CBC

XE-5000 Technology Provides Clinically Relevant,Reportable Parameters:

• NRBC – Fluorescent NRBC count with excellent sensitivity and specificity

• PLT-O – Fluorescent optical platelet count and traditional impedancePLT counting to improve accuracy of very low and very high PLT counts

• Retic – Fluorescent reticulocyte count to reduce manual confirmationmethods and their inherent errors

• RET-He – Reticulocyte Hemoglobin Content measures the incorporationof iron into the red cell to assist in anemia evaluation and management(e.g., functional iron deficiency anemia)

• IPF – Immature platelet fraction (measurement of reticulated platelets)to monitor thrombopoietic activity of the marrow

• HPC –Quantitative hematopoietic progenitor cell count as a screenfor the optimal presence of hematopoietic progenitor cells in peripheralblood and cord blood samples

The IG Count provides:

Improved accuracy and sensitivity:• Reduced false positive and false

negative rates• Result consistency by reducing

tech-to-tech variation in reportingmanual differentials

Improved workflow:• Fewer manual differentials means

faster turnaround time (TAT)

Labor Savings:• Fewer manual slide reviews

improves laboratory efficiency andsupports higher laboratory output.

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Fluorescent Nucleated Red Blood Cells

Immature Granulocyte Count

Immature Granulocyte Count

The Sysmex XE-5000 offers a 6-partdifferential, which includes an ImmatureGranulocyte Count (IG%, #). The IGcount offers reportable, quantitativeresults for immature granulocytes(metamyelocytes, myelocytes andpromyelocytes).

Advanced Clinical Parameters

Fluorescent Reticulocyte Count

Known as the “Gold Standard” in reticulocyte testing, the fluorescentreticulocyte count is available on the XE-5000. Sysmex provides on-boardretic testing in a dedicated channel, improving your efficiency in reportingreticulocytes 24 hours per day.

With the use of fluorescent technology, the retic channel assures:

• Accurate reticulocyte % and #• Improved immature reticulocyte information for earlier diagnosis

and treatment by clinicians• Elimination of common interferences from Howell-Jolly bodies,

Pappenheimer bodies and immature reticulocytes to avoidmanual counts

Reticulocyte HemoglobinMeasurement (RET-He)

The Reticulocyte Hemoglobin (RET- He) is a parametermeasured in the reticulocyte channel and is used to measure theincorporation of iron into erythrocyte hemoglobin.

The RET-He parameter supports:

• Rapid, direct analysis of an earlier stage of RBC development forprompt clinical follow-up

• Assessment of anemia and is an established parameter used inKDOQI (Kidney Disease Outcomes Quality Initiative) guidelinesfor assessing the initial iron status of patients

• Accuracy and sensitivity in measurement of red cell productionthat supports effective monitoring of costly drug protocols forcell stimulation

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Fluorescent Reticulocyte Count

Fluorescent Optical Platelet

The XE-5000 offers a fluorescentoptical platelet along with thetraditional impedance platelet count.These dual technologies maximizeinstrument output. Flagging associatedwith atypical or abnormal platelets,due to increased size or fragmentation,is minimized by use of the opticalplatelet count. The accuracy inreporting is supported by theavailability of both technologies.

Fluorescent Optical Platelet (PLT-O) isa complementary, reportable parameterto the impedance platelet count.

The PLT-O parameter provides:

• Improved accuracy on lowplatelet counts

• Accurate counts when interferencesare present, thus reducingmanual intervention

• Automated judgment for reportingPLT-O or impedance PLT throughinstrument settings, eliminatingtech-to-tech decision variability

Fluorescent Optical Platelet

One Comprehensive System

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Hematopoietic Progenitor Cell Counts

HematopoieticProgenitor Cell Counts

The Sysmex XE-5000 offers aquantitative hematopoietic progenitor(HPC) cell count as a screen for themeasurement of the optimal time forcell harvest. The Sysmex automatedHPC count is substantially equivalentto CFU and CD34+.

The HPC count from peripheral bloodand cord blood samples supports:

• Quality screening information tobetter assist clinicians with patienttreatment protocols

• Rapid analysis versus conventionalmethods, thereby allowing for aprompt determination of optimalstem cell harvest time

• Automation of manual processes• Cost reduction as the system

does not require additionalreagents, sample volume orspecific technical expertise

Immature Platelet Fraction

The Immature Platelet Fraction(IPF %) is a parameter used toassess thrombopoiesis.

IPF offers:

• Accuracy and sensitivity inmeasurement of immature platelets

• Delineation of abnormal plateletpopulations seen by clinicians indisease states such as AITP(autoimmune thrombocytopenicpurpura) or TTP (thromboticthrombocytopenic purpura)

• Rapid reporting of results forprompt clinical information thatcan potentially avoid invasive andcostly procedures

XE-5000 Body FluidMode

The XE-5000 analyzer includes abody fluid specific mode. This modeprovides a reportable RBC, WBC, WBCdifferential (polymorphonuclear andmononuclear) and a total count (TC-BF)for all common body fluid samples(CSF, synovial and serous). The analyzerapplies proven impedance andfluorescent flow cytometry, ensuringan accurate body fluid count.

The XE-5000 Body FluidCount Provides:

• Expanded sensitivity and linearity –WBC and RBC counts reportable to3 decimal places

• 2-Part Differential• No sample pretreatment• No additional reagents• An automatic background check

prior to analyzing a sample

The XE-5000 Body FluidCount Benefits:

• Improved productivity• Decreased turnaround time (TAT)• Decreased manual technical

intervention

Body Fluid DifferentialImmature Platelet Fraction

Improved Productivity & EfficiencyMaximizing Your Quality and Uptime

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Productivity

The system provides a throughputof up to 150 samples per hour,producing high-quality resultsrapidly for clinicians to use indiagnosis and treatment decisions.

• The system is compatible withSysmex automation platforms,providing solutions that canbe scalable to meet needs ofall laboratories.

• XE-5000 can also be used inconjunction with Sysmex WAM™Decision Support Software for theClinical Laboratory, which enhancessample and data workflow andimproves turnaround time (TAT).

Excellent Performance

• Reliable platform• Low sample volume requirements• SNCS™ (Sysmex Network

Communications System) remotemonitoring systemsupporting maximum uptime

• Ranked by third party ashighest vendor for reliability for9 consecutive years*

*IMV ServiceTrak 2009

Reportable, DiagnosticInformation from aSingle Sample Analysis

• 6-part whole blood WBCdifferential (NE + Lymph + Mono +Eo + Baso + IG) with fluorescentNRBC count

• Body fluid cell count and differential• Anemia evaluation parameters –

Reticulocyte, IRF, and RET-He

• Optical and impedance plateletmeasurement capabilities

• Thrombopoietic activity assessmentparameters (IPF)

• Quantitative hematopoieticprogenitor cell count (HPC)

Easy-to-use

• Intuitive software menus• On-board help key for rapid

troubleshooting• Comprehensive quality control

information• Barcoded reagent management

Improved Patient Care

An additional benefit to the XE-5000analyzer is the ability to analyze patientsamples when only small volumes ofsample are available. The capillary mode(pre-dilute analysis mode) requiring only40 μL of whole blood provides qualityclinical data rapidly and consistently.Results from the capillary mode includesCBC, 6-part differential, retic and NRBC.

Your Complete Choice

The XE-5000 offers a comprehensiveclinical testing menu for both wholeblood and body fluids, providingaccurate, precise and sensitiveresults. With its rapid throughput,your physicians receive quality,clinically relevant information toassist in the management anddiagnosis of all patients.

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Principles & Technologies Fluorescent Flow Cytometry:WBC-Diff, IG, NRBC, RET, IRF, PLT-O

RF-DC method:HPC

DC-Sheath-Flow:PLT, RBC, HCT

SLS-Method:Hgb

31 Whole Blood Reportable WBC, RBC, HGB, HCT, MCV, MCH, MCHC,Parameters PLT (Impedance and Fluorescent Optical)

NEUT%, LYMPH%, MONO%, EO%, BASO%,NRBC%, NEUT#, LYMPH#, MONO#, EO#,BASO#, NRBC#, IG%, IG#, HPC#, RDW-SD,RDW-CV, MPV, RET%, RET#, IRF, RET-He, IPF

7 Body Fluid Reportable Parameters WBC-BF, RBC-BF, MN%, MN#, PMN%,PMN#, TC-BF#

Linearity WBC: 0 – 440 x 103/µLRBC: 0 – 8 x 106/µLPLT: 0 – 5 x 106/µL

Body Fluid Linearity WBC-BF: 0.000 – 10.000 x 103/µLTC-BF: 0.000 – 10.000 x 103/µLRBC-BF: 0.000 – 5.000 x 106/µL

Throughput Whole Blood Mode:150 samples/hr (max.)

Body Fluid Mode:38 samples/hr (max.)

Sample Volumes 200μL / 130μL (closed/open mode)40μL for capillary mode

Data Storage 10,000 samples (incl. graphics)(IPU: Information Processing Unit)

Quality Control Levey-Jennings control chart(Total QCManagement) X-bar M file

Comprehensive QC files including“current” and “new” lot feature

Online QC

Interfaces Sysmex WAMTM (HL7 & ASTM)

Dimensions / Weight Main unit: 27.8" x 35.9" x 28" / 178 lbs.w x h x d [in] / [lbs] Sampler: 22.8" x 7.7" x 14.8" / 26 lbs.

IPU: 18" x 17.6" x 5.3" / 24 lbs.

Configurations StandaloneSysmex HST-N, AlphaN Automation

SysmexXE-5000 Specifications

Sysmex UK, Ltd.Sysmex House, Garamond Drive, WymbushMilton Keynes, Buckinghamshire, Mk8 8DF, U.K.Tel. +44 (870) 9029270Fax +44 (870) 9029211www.sysmex.co.uk

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Sysmex do Brasil Indústria e Comércio LtdaRua Joaquim Nabuco, 615 - Bairro Cidade Jardim,São José dos PinhaisParaná – Brasil – CEP 83040-210Tel. +55 (41) 2104-1314Fax +55 (41) 2104-1300www.sysmex.com.br

Sysmex Europe GMBHBornbarch 1, 22848,Norderstedt, GermanyTel. +49 (40) 52726-0Fax +49 (40) 52726-100www.sysmex-europe.com

Sysmex Deutschland GMBHBornbarch 1, 22848,Norderstedt, GermanyTel. +49 (40) 5341020Fax +49 (40) 5232302www.sysmex.de

© 2010 Sysmex America, Inc.Document Number MKT-10-1126 06/2010