World Renowned HPLC Mediaaz621941.vo.msecnd.net/documents/8a859e9b-4cb9-4d2a-a620-55e… · World...

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World Renowned HPLC MediaReinvented for UHPLC!

New Luna Omega 1.6 µm • Staggering UHPLC Efficiencies and Performance

• Extreme Ruggedness and Mechanical Strength

• Perfect Complement to Kinetex® Core-Shell Technology

NEWPolar C18 and C18 Selectivities

2

Enhanced with 20 Years of Technology,

Innovation, and Experience

One of the world’s leading HPLC brands, now enhanced for incredible UHPLC performance! Luna Omega 1.6 µm UHPLC columns culminate 20 years of technological prowess, advancements, and innovation from Phenomenex!

With astounding efficiency levels, highly versatile selectivities, and trusted accuracy, Luna Omega columns will take your UHPLC

experience to a new level.

Luna® Omega UHPLC columns will boost your UHPLC instrumentation!

3

Cutting Edge UHPLC ..................................................................... p. 4

Astounding Performance .............................................................. p. 5

Separation Muscle ......................................................................... p. 6

Inert Foundation ............................................................................. p. 7

Excellent Reproducibility ........................................................ pp. 8-9

C18 Selectivity .............................................................................. p. 10

Utilizing Hydrophobicity .............................................................. p. 11

Polar C18 Selectivity .................................................................... p. 12

Enhanced Polar Retention ......................................................... p. 13

100% Aqueous Stability .............................................................. p. 14

Polar and Non-polar Selectivity ................................................. p. 15

Upgrading C18 Methods ............................................................. p. 16

Improving Existing Polar Methods ............................................. p. 17

Polar Case Study: Catecholamines and PMETs ....................... p. 18

Polar Case Study: ETG/ETS ........................................................ p. 19

C18 and Polar C18 - Complementary Work Horses ........ pp. 20-21

Pairing With Core-Shell Technology .................................. pp. 22-23

Column Lifetime ........................................................................... p. 24

Sample Preparation ..................................................................... p. 25

Column Protection – Guard Cartridge System ........................ p. 26

Ordering Information ................................................................... p. 27

Why Should Luna® Omega be in your lab

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© 2016 Phenomenex, Inc. All rights reserved.

Phenomenex l WEB: www.phenomenex.com

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Within the novel manufacturing process of Luna Omega 1.6 µm silica, we implement a proprietary processing technique to gain greater particle inertness, a stronger particle morphology, and more consistent porosity.

Most importantly, through our proprietary process, we eliminate micropores, further improving column efficiency, inertness, and reproducibility.

Consistent Porosity

Absence of Micropores

Luna® is one of the most recognized HPLC brands on the market, delivering high efficiency, ruggedness, repro-ducibility and dependability for a wide range of analyses. The new Luna Omega 1.6 μm builds upon this legacy with an innovative yet rugged UHPLC silica particle architecture, designed and manufactured by Phenomenex based on more than 20 years of applied knowledge, invention, and customer experience.

Novel Design and Manufacturing Process

Cutting-Edge 1.6 μm Silica Particle

TMSTMS

PolarPolar

TMSTMS

Thermal Modified Pore

Structure

Luna Omega C18

Luna Omega Polar C18


5

Consistent Porosity

Absence of Micropores

The undeniably high efficiency levels found in each Luna Omega UHPLC column provide you with the potential of huge gains in method performance. While traditional silica and hy-brid fully porous particles claim high performance, when compared to Luna Omega 1.6 μm, they drastically fall short and prevent UHPLC scientists from reaching their UHPLC potential.

UHPLC Efficiency Comparison

Comparative separations may not be representative of all applications.

Efficiency (plates/meter)

Luna® Omega 1.6 µm C18

Thermo Scientific® Hypersil GOLD® 1.9 µm C18

Agilent® ZORBAX® Eclipse 1.8 µm XDB-C18

Thermo Scientific Syncronis™ 1.7 µm C18

Waters® ACQUITY® HSS 1.8 µm C18

Waters ACQUITY BEH 1.7 µm C18

AkzoNobel® Kromasil® 1.8 µm C18

ACE® Excel™ 2 µm C18

YMC®-Triart 1.9 µm C18

Agilent ZORBAX 1.8 µm SB-C18

0 50,000 100,000 150,000 200,000 250,000 300,000 350,000

Conditions for all columns:Dimension: 50 x 2.1 mm

Mobile Phase: Acetonitrile/Water (65:35)Flow Rate: 0.5 mL/min

Temperature: AmbientDetection: UV @ 254 nm

System: ACQUITY UPLC®

Astounding PerformanceCutting-Edge 1.6 μm Silica Particle


6

01.0e52.0e53.0e54.0e55.0e56.0e57.0e58.0e59.0e51.0e61.1e61.2e61.3e61.4e61.5e61.6e6

1 2 3 4 5 6 min

23441

1 2 3 4 5 6 min0.0

1.0e52.0e53.0e54.0e55.0e56.0e57.0e58.0e59.0e51.0e61.1e61.2e61.3e61.4e61.5e61.6e6

5.0e41.0e51.5e52.0e52.5e53.0e53.5e54.0e54.5e55.0e55.5e56.0e56.5e57.0e57.5e5

1 2 3 4 5 6 min0.0

23440

Our industry leading bonding technologies in conjunction with high efficiency levels ensure excellent stationary phase coverage and improved separation power. Now, with Luna Omega 1.6 μm, you can turn difficult separations into resolution achievements.


Conditions for all columns:Columns: Luna Omega 1.6 µm C18

ZORBAX 1.8 µm XDB-C18ACE Excel 2 µm C18-AR

Dimension: 50 x 2.1 mmMobile Phase: A: 0.1 % Formic Acid in Water

B: 0.1 % Formic Acid in MethanolGradient: Time (min)

07

% B390

Flow Rate: 0.3 mL/minTemperature: 30 °C

Detection: MS/MSSample: 1. Succinic acid

2. MMA3. Glutaric acid4. Methylsuccinate5. Ethylmalonic acid6. Hippuric acid7. Homovanillic acid8. Suberic acid


ACE® Excel™ 2 µm C18-AR

Agilent® ZORBAX® 1.8 µm XDB-C18

Ap

p ID

234

39A

pp

ID 2

3441

Ap

p ID

234

40

Inte

nsity

, cp

sIn

tens

ity, c

ps

Inte

nsity

, cp

s

Separation Muscle

Much Greater Resolution!


7

0.05

0.1

0.15

0.2

0.25AU

0

min0 1 2 3 4

1

4

5,2

6

Inert Foundation

Luna Omega UHPLC columns contain a unique silica modified using a propri-etary, post-synthetic thermal treatment process to provide extraordinary me-chanical strength and significantly greater inertness than traditional fully porous and hybrid materials. This greatly minimizes secondary interactions that nega-tively affect peak shape, allowing for greater method accuracy.

Waters® ACQUITY® BEH 1.7 µm C18




ACQUITY BEH 1.7 µm C18Hypersil GOLD 1.9 µm C18

Dimension: 50 x 2.1 mmMobile Phase: A: 0.1 % Formic Acid in Water

B: 0.1 % Formic Acid in AcetonitrileGradient: Time (min)

056

6.18

% B5959555

Flow Rate: 0.4 mL/minTemperature: Ambient

Detection: UV @ 254 nmSample: 1. Pindolol

2. Chlormipramine3. Nortripytline 4. 3-Methyl-4-nitrobenzoic acid5. 5-Methylsalicylate6. Hexanophenone


Poor Peak Shape

Separation Muscle

0.025

0.05

0.075

0.1

0.125

0.15

0.175

AU

0

min0 1

1

2

3

4

5

6

2 3 4

Excellent Peak Shape

Ap

p ID

234

29A

pp

ID 2

3431

0.05

0.1

0.15

0.2

min0 1 2 3 4

AU

0

1

2

3

4

5

6

Ap

p ID

234

30

Tailing Has Caused Poor Quantitation

Peak #3 is Adsorbed


8

Consistent Results – C18

Batch-to-batch and column-to-column, Luna® Omega media and columns are designed to be consistent and incredibly accurate tools for your analysis. Each batch and column are quality tested to ensure dependability and reproducibility.

Luna Omega C18 – Batch A

0

0.2

0.4

0.6

0.8

1

1.2

1.4

10 2 3 4 5 min

1

23 4

56

7

8

Ap

p ID

235

23

Luna Omega C18 – Batch B

0

0.2

0.4

0.6

0.8

1

1.2

1.4

10 2 3 4 5 min

1

23 4

56

7

8A

pp

ID 2

3523

Luna Omega C18 – Batch C

0

0.2

0.4

0.6

0.8

1

1.2

1.4

10 2 3 4 5 min

1

23 4

56

7

8

Ap

p ID

235

23


Dimension: 50 x 2.1 mmPart No.: 00B-4742-AN

Mobile Phase: A: WaterB: Acetonitrile

Gradient: Time (min)06

6.018

% B20602020


Detection: UV @ 220 nmSample: 1. Estriol

2. Prednisolone3. Hydrocortisone4. Cortisone5. Cortisone Acetate6. 21-Hydroxycortisone7. 17-Hydroxycortisone8. Deoxycorticosterone

Peak Capacity

270

Peak Capacity

263

Peak Capacity

270


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Consistent Results – Polar C18

Just like Luna® Omega C18, the Luna Omega Polar C18 is also stringently test-ed to make sure both batch-to-batch and column-to-column quality stays at the highest level before it reaches your lab bench.

Luna Omega Polar C18 – Batch A

25

0

50

75

100

120

100

150

175

10 2 3 4 min

12

34

5 6

7

Ap

p ID

235

70

Luna Omega Polar C18 – Batch B

25

0

50

75

100

120

100

150

175

10 2 3 4 min

1 2

34

5 6

7A

pp

ID 2

3570

Luna Omega Polar C18 – Batch C

25

0

50

75

100

120

100

150

175

10 2 3 4 min

1 2

34

5 6

7

Ap

p ID

235

70

Conditions for all columns:Columns: Luna Omega 1.6 µm Polar C18


Mobile Phase: A: 0.1 % Formic Acid in WaterB: 0.1 % Formic Acid in Acetonitrile

Gradient: Time (min)0 5

% B595


Detection: UV @ 254 nmSample: 1. Uracil

2. Pindolol 3. Chlorpheniramine 4. Nortriptyline 5. 3-Methyl-4-nitrobenzoic acid 6. 5-Methylsalicylate7. Hexanophenone

Peak Capacity

163

Peak Capacity

166

Peak Capacity

162

QUALITYMANAGEMENT SYSTEMCERTIFIED BY DNV GL

9001:2008


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Selectivity Highlight Luna Omega C18

Luna® Omega C18 is an excellent first choice for chromatographers who are just starting method de-velopment or attempting to improve upon existing chromatographic results with other C18s. With its higher performance potential, excellent retention profile, and greater inertness, the Luna Omega C18 was designed to be the new all-purpose UHPLC solution for industries all over the world.

Phase C18

Particle Size 1.6 µm

Pore Size 100 Å

pH Range 1.5 - 8.5*

Surface Area 260 m2/g

Carbon Load 11 %

Pressure Limit 1000 bar

USP Listing L1

Greater Retention and Better ResultsHigher efficiency levels in combination with excellent stationary phase coverage and greater particle inert-ness, translates to improved separation power for you. Now you can utilize the greater retention of Luna Omega C18 to tackle both easy and difficult separations.

Luna Omega 1.6 µm C18


min0 0.5 1 1.5 2 2.5

AU

0

11

2 3

4

5 6

7

9

8

min0 0.5 1 1.5 2 2.5

AU

0

1

12

3

4

5 6

7

9

8

Ap

p ID

234

85

Ap

p ID

234

82

*pH stability under gradient conditions. pH stability is 1.5-10 under isocratic conditions.

TMSTMS

Conditions for all columns:Columns: Luna Omega 1.6 μm C18

ACQUITY BEH 1.7 μm C18Dimension: 50 x 2.1 mm


Gradient: Time (min)0 3

3.5 3.51

5

% B10 55 55 10 10


Detection: UV @ 205 nmSample: 1. Acetaminophen

2. 4-Aminobenzoic Acid3. 4-Hydroxybenzoic Acid4. 2-Acetaminophenol5. 3-Hydroxybenzoic Acid6. Salicylicamide7. Phenol8. Benzoic Acid9. Salicylic Acid

Peak Capacity

196

Peak Capacity

160

Greater Peak Capacity and Retention



11

Small and Large Compound Mixtures

Strong and focused hydrophobic retention, incredible efficiency and valuable in-ertness of Luna® Omega C18 columns make them an excellent choice for small mixtures of compounds differing in hydrophobicity as well as large mixtures of compounds like impurity/degradation profiles and peptide maps.

Phenols

OTC Drug - Pill Formulation Profile

Synthetic Cannabinoids

Peptide Map – Digested BSA

AU

-0.005

0

0.005

0.01

0.015

0.02

0.025

min0 1

1

2

2

3

3

45

67

4 50.0

1.0e52.0e53.0e54.0e55.0e56.0e57.0e58.0e59.0e51.0e61.1e61.2e61.3e61.4e6

3 4 5 6 7 8 9 min

Ap

p ID

234

42

Columns: Luna Omega 1.6 µm C18Dimension: 50 x 2.1 mm

Part No.: 00B-4742-ANMobile Phase: A: 0.1 % Formic Acid in Water


067

7.19

% B5505055


Detection: UV @ 270 nmSample: 1. 3-Hydroxyphenol

2. Phenol3. 4-Nitrophenol4. 4-Methylphenol5. 2-Methylphenol6. 2,4-Dimethylphenol7. 1-Naphthol


Part No.: 00B-4742-ANMobile Phase: A: 0.1 % Formic Acid in Water


06

% B50100


Detection: MS/MS (SCIEX API 4000™)Sample: 1. JWH-073 Butanoic acid metabolite

2. JWH-073 3-hydroxybutyl metabolite 3. JWH-018 Pentanoic acid metabolite 4. JWH-073 4-hydroxybutyl metabolite 5. JWH-018 4-hydroxypentyl metabolite 6. AM-2201 4-hydroxypentyl metabolite 7. JWH-018 5-hydroxypentyl metabolite 8. AM-694 9. AM-220110. JWH-073 11. JWH-018

Ap

p ID

234

34

0.01

0.02

0.03

0.04

0

AU

0 1 2 3 4 min0.00

1.00e82.00e83.00e84.00e85.00e86.00e87.00e88.00e89.00e81.00e91.10e9

10 20 30 min

Ap

p ID

236

00


Part No.: 00B-4742-ANMobile Phase: A: 20 mM Potassium Phosphate pH 7.2

B: AcetonitrileGradient: Time (min)

056

6.18

% B5707055


Detection: UV @ 254 nmSample: OTC Drug Pill


Part No.: 00D-4742-ANMobile Phase: A: 0.1 % Formic Acid in Water


050

50.1

% B3503


Detection: MS/MS (SCIEX API 4000)Sample: Tryptic digest of BSA

Ap

p ID

235

17

Inte

nsity

, cp

s



12

Selectivity Highlight Luna Omega Polar C18

Luna® Omega Polar C18 is a novel UHPLC station-ary phase capable of providing a unique selectivity within a wide elution window and increased retention for both polar and non-polar analytes. The all-pur-pose C18 ligand provides hydrophobic interactions while a polar modified particle surface provides en-hanced polar retention and also aqueous stability. These attributes make the Luna Omega Polar C18 an excellent choice for balanced retention of polar and hydrophobic compounds as well as to solely enhance retention of highly polar compounds.

Phase Polar C18

Particle Size 1.6 µm

Pore Size 100 Å

pH Range 1.5 - 8.5*

Surface Area 260 m2/g

Carbon Load 9 %

Pressure Limit 1000 bar

USP Listing L1

A C18, But DifferentLuna Omega Polar C18 is a uniquely modified C18-based chemistry that has been optimized to improve the performance of polar analyses. This new particle surface chemistry makes the Polar C18 applicable to all industries that utilize UHPLC for mixtures of polar and non-polar compounds.

Luna Omega C18 silica surface

TMSTMS

Luna Omega Polar C18 silica surface

TMS

TMS

*pH stability under gradient conditions. pH stability is 1.5-10 under isocratic conditions.

PolarPolar

TMSTMS

PolarPolar


13

Greater Polar Retention

With its polar modified surface, the Luna® Omega Polar C18 offers UHPLC users enhanced separation power that can greatly improve resolution values for target compounds.

Nicotine and Metabolites

Catecholamines

02.0e5

2 min1

4.0e56.0e58.0e51.0e61.2e61.4e61.6e61.8e62.0e62.2e62.4e62.6e62.8e6

1+2 3

4

5

1.0e5

2.0e5

3.0e64.0e6

5.0e6

6.0e67.0e6

8.0e6

02 min

2

3 4

5

1

1

2.0e54.0e56.0e58.0e61.0e61.2e61.4e61.6e61.8e62.0e62.2e62.4e6

02 min1

1+23

4

5

1.0e5

2.0e5

3.0e6

4.0e6

5.0e6

6.0e6

7.0e6

8.0e6

02 min

2

34

5

1

1

Ap

p ID

235

95A

pp

ID 2

3596


ACQUITY BEH 1.7 µm C18Dimension: 100 x 2.1 mm

Mobile Phase: A: Water with 0.1% Formic AcidB: Acetonitrile with 0.1% Formic Acid


3.1

% B0900


Detection: MS/MS (SCIEX API 4000)Sample: 1. Norepinephrine

2. Epinephrine3. Normetanephrine4. Dopamine5. Metanephrine

Ap

p ID

235

94A

pp

ID 2

3591

Inte

nsity

, cp

sIn

tens

ity, c

ps

Inte

nsity

, cp

sIn

tens

ity, c

ps


CORTECS 1.6 µm C18Dimension: 50 x 2.1 mm

Mobile Phase: A: 10 mM Ammonium Formate with 0.1% Formic AcidB: Acetonitrile with 0.1% Formic Acid


3.1

% B2902


Detection: MS/MS (SCIEX API 4000™)Sample: 1. Nornicotine

2. 3-Hydroxycotinine3. Nicotine4. Cotinine5. Anabasine

Greater Retention

Greater Retention

Luna Omega 1.6 μm Polar C18

Waters® CORTECS®

1.6 µm C18


Luna Omega 1.6 µm Polar C18



14

100 % Aqueous Stability

No Stationary Phase CollapseTraditional C18 phases are known to collapse under 100% aqueous conditions, causing retention loss of compounds and a method development headaches. That is why an advanced proprietary bonding technology was used for the Luna® Omega Polar C18 in order to ensure aqueous stability. The graph below displays the excellent stability of Polar C18 in 100% aqueous buffer conditions for over 2 weeks.



Mobile Phase: 10 mM Ammonium Formate with 0.1 % Formic Acid

Flow Rate: 0.4 mL/min

Temperature: 22 °CDetection: UV @ 254 nm

Sample: 1. Adenine2. Guanosine-5-Monophosphate3. Thymine

Aqueous Stability of Luna Omega Polar C18

% o

f Ini

tial R

eten

tion

Tim

e

Adenine G-5-MP Thymine

100%110%

80%

60%

40%

20%

0%

Day 1

Day 25

Nucleosides in 100 % Aqueous Conditions



Mobile Phase: 20 mM Ammonium Formate pH 3.0Flow Rate: 0.4 mL/min

Temperature: 22 °C

Detection: UV @ 285 nmSample: 1. Thiourea

2. 5-Fluorocytosine3. Guanosine-5-Monophosphate4. Adenine5. Thymine

0.080

0.0100.020

0.030

0.040

0.050

0.060

0.070

1

2

3 4 5

min0 1 2

0

0.080

0.0100.020

0.030

0.040

0.050

0.060

0.070

1

2

34 5

min0 1 2

0

23585

Ap

p ID

235

85

Ap

p ID

235

86

Ap

p ID

235

77

Use 100% aqueous conditions for the greatest polar acid/base retention.




Duration of Exposure


15

100 % Aqueous Stability

0

20

40

60

80

100

0

20

40

60

80

100

0

20

40

60

80

100

0

20

40

60

80

100

0 2 4 6 8 10 min

RT: 0.00 - 12.00

RT: 2.97AA: 96610SN: 1654

RT: 1.84AA: 7670SN: 36

RT: 1.93AA: 508SN: 4

RT: 2.68AA: 384SN: 10

RT: 8.23AA: 847097SN: 2491

RT: 9.77AA: 313SN: 10

RT: 7.62AA: 3628908SN: 4156

RT: 9.17AA: 167SN: 13

NL: 7.27E3TIC F: + c ESI SRM ms2 115.000 [98.099-98.101] MS ICIS Test03b



RT: 1.32AA: 373SN: 34

RT: 4.64AA: 179SN: 35

NL: 3.83E3TIC F: + c ESI SRM ms2 416.000 [194.099-194.101]MS ICIS Test03b

Versatility – Hydrophobic and Polar Interactions

Combined retention of polars and non-polars by the Luna® Omega Polar C18 gives you an incredible range of potential success when working with methods from just about any industry. Here we illustrate the useful-ness of these interaction mechanisms with water soluble vitamins from drink and tablet, a multi-compound antibiotic screen, and a UHPLC/MS/MS separation of algal toxins.

Antibiotic Screen

Find complete method at Phenomenex.comApplication ID 23569

1.0e6

2.0e6

3.0e6

4.0e6

min0 1 2 3 4 50

Ap

p ID

235

87



Mobile Phase: A: 10 mM Ammonium Formate with 0.1 % Formic AcidB: Acetonitrile with 0.1 % Formic Acid


4.17

% B09000


Detection: MS/MS (SCIEX API 4000™)Sample: 1. Pyridoxamine

2. Thiamine3. Nicotinic acid4. Pyridoxine

5. Pantothenic acid6. Folic acid7. Riboflavin

Inte

nsity

, cp

s

2.0e5

1.0e5

3.0e5

5.0e56.0e5

4.0e5

2 3 4 min10

2 3

4

5

1.0e6

2.0e6

3.0e6

4.0e6

4.5e6

2 3 4 min10

2

4

5

6 71

Ap

p ID

235

78A

pp

ID 2

3579

Inte

nsity

, cp

s

Rel

ativ

e A

bun

dan

ce

Inte

nsity

, cp

s

02.0e54.0e56.0e58.0e51.0e61.2e61.4e61.6e61.8e62.0e6

2

2

3

3

4

4

56

7

min1

1

Ap

p ID

235

84

Standards

Uracil-d4

Vitamin Enriched Drink

Anatoxin-a

Vitamin Tablet

L-Phenylalanine-d5

Cylindrospermopsin

Columns: Luna Omega 1.6 µm Polar C18Dimension: 50 x 2.1 mm

Part No.: 00B-4748-ANMobile Phase: A: 10 mM Ammonium formate with

0.1 % Formic AcidB: Acetonitrile with 0.1 % Formic Acid


0.55

5.1

% B09500


Detection: MS/MS (SCIEX API 4000)

Water Soluble Vitamins Algal Toxins (EPA 545)


1. Sulfaguanidine 2. Sulfadiazine 3. Cefalexin 4. Lincomycin 5. Amoxicillin 6. Sulfathiazole 7. Sulfapyridine 8. Cefazolin 9. Sulfamerazine 10. Ciprofloxacin 11. Sulfamethazine 12. Sulfamonomethoxine 13. Enrofloxacin 14. Difloxacin 15. Chlortetracycline 16. Sulfamethoxazole 17. Florfenicol 18. Sulfaquinoxaline 19. Oxacillin 20. Sulfadimethoxine


16

Highly Useful Alternative to the Common C18

Unlike traditional UHPLC C18 stationary phases, the polar and hydrophobic ver-satility of Polar C18 combined with its high efficiency levels allow for enhanced separation power. Combine that with 100 % aqueous stability and you can really see how the Luna® Omega Polar C18 can potentially lead to more separation success compared to traditional C18 columns.

Ketotifen Impurity Profile

Beta Blockers

0.005

0.010

0.015

0.020

0.025

0.030

0

2 3 4 5 min1

0.005

0.010

0.015

0.020

0.025

0.030

0.010

0.015

0.020

0.025

0.030

0.035

02 3 4 5 min1

0.005

2

3

45

6

1

0.005

0.010

0.015

0.020

0.025

0.030

0

2 3 4 5 min1

0.005

0.010

0.015

0.020

0.025

0.030

0.010

0.015

0.020

0.025

0.030

0.035

0

0.005

2

2

3

3

4

4

5

56

min1

1

Ap

p ID

236

05A

pp

ID 2

3612


Hypersil GOLD 1.9 µm C18Dimension: 50 x 2.1 mm

Mobile Phase: A: Water with 0.1 % Formic AcidB: Acetonitrile with 0.1 % Formic Acid


2.5610

10.0113

% B515203055


Detection: UV @ 254 nmSample: Ketotifen


ZORBAX 1.8 µm SB-C18Dimension: 100 x 2.1 mm

Mobile Phase: A: 20 mM Postassium Phosphate pH 7.2B: Acetonitrile


2.5

% B535


Detection: UV @ 280 nmSample: 1. Atenolol

2. Pindolol3. Timolol4. Metoprolol5. Labetolol6. Propranolol

Ap

p ID

236

04A

pp

ID 2

3611

Inte

nsity

, cp

sIn

tens

ity, c

ps

Inte

nsity

, cp

sIn

tens

ity, c

ps




Agilent® ZORBAX® 1.8 µm SB-C18

Clean, sharp peaks and excellent resolution

Greater retention and resolution of impurities



17

Excellent Alternative to Existing Polar Columns

Luna® Omega Polar C18 is an incredibly high performing UHPLC column with a unique selectivity profile that can be used to upgrade existing methods or jumpstart new method development. Realize lower limits of detection with the increase in sensitivity levels or just utilize the efficiency gains to help resolve closely eluting peaks.

Mycotoxins Screen

SAMSHA Workplace Drug Testing Screen

2.0e5

3.0e5

4.0e5

5.0e5

6.0e5

1.0e5

0

1

3+4

256

78

1 2 3 4 5 6 min

2.0e5

3.0e5

4.0e5

5.0e5

6.0e5

1.0e5

01 2 3 4 5 6 min

23571

2

3+4

5

67 8

1

2.0e5

0

4.0e5

6.0e5

8.0e5

1.0e6

1.2e6

1.4e6

1.6e61.8e6

1 2 3 4 min

23589

2.0e5

0

4.0e5

6.0e5

8.0e5

1.0e6

1.2e6

1.4e6

1.6e61.8e6

23588

1 2 3 4 min

Ap

p ID

235

72

Ap

p ID

235

88

Ap

p ID

235

89

Conditions same for all columns:Columns: Luna Omega 1.6 µm Polar C18

ACQUITY HSS 1.8 µm T3Dimension: 50 x 2.1 mm



3.1

% B1510010015


Detection: MS/MS (SCIEX API 4000)Sample: 1. Morphine

2. Codeine 3. Amphetamine 4. MDA 5. Methamphetamine 6. 6-MAM 7. MDMA 8. MDEA 9. BZE 10. PCP 11. THC-COOH 12. THC

Conditions same for all columns:Columns: Luna Omega 1.6 µm Polar C18

ACQUITY HSS 1.8 µm T3Dimension: 50 x 2.1 mm



4.16

% B20902020


Detection: MS/MS (SCIEX API 4000™)Sample: Positive Mode (ESI+)

1. 15-Acetyldeoxynivalenol2. Aflatoxin G23. Aflatoxin G14. Aflatoxin B25. Aflatoxin B16. Aflatoxin M17. HT2 Toxin8. T2 Toxin

Ap

p ID

235

71

Inte

nsity

, cp

s

Inte

nsity

, cp

s

Inte

nsity

, cp

s


Waters® ACQUITY® HSS 1.8 µm T3


Waters® ACQUITY® HSS 1.8 µm T3



18

Polar Case Study – Catecholamines and Metabolites

Current analytical tests for pheochromocytoma (tumor of chromaffin cells of the adrenal medulla) focus on measuring for elevated levels of plasma free metanephrine and normetanephrine, which are continually se-creted by the tumors. Supplementary methods also analyze the metabolites of metanephrine and normeta-nephrine, including vanillylmandelic acid. With such a range of polar compounds, the aqueous stability and enhanced polar retention of the Luna® Omega Polar C18, both play an strong part in generating excellent separation between all these important analytes.

Catecholamines (ESI+)

PMETs from Plasma (ESI+)

Catecholamine Metabolites (ESI-)Flow Rate: 0.3 mL/min

Ap

p ID

235

91A

pp

ID 2

3592

Ap

p ID

235

93

Inte

nsity

, cp

sIn

tens

ity, c

ps

Inte

nsity

, cp

s

1.0e5

2.0e5

3.0e6

4.0e6

5.0e6

6.0e6

7.0e6

8.0e6

02

2

3

3 4

5

6

4 min1

1

1.0e5

2.0e5

3.0e6

4.0e6

5.0e6

6.0e6

7.0e6

8.0e6

02 3

34

5

6

9

4 min1

1.0e5

2.0e5

3.0e6

4.0e6

5.0e6

02

8

7

9

3 min1

Conditions for all columns unless otherwise noted:Columns: Luna Omega 1.6 µm Polar C18

Dimension: 100 x 2.1 mmPart No.: 00D-4748-AN

Mobile Phase: A: Water with 0.1 % Formic AcidB: Acetonitrile with 0.1 % Formic acid


3.1

% B0900



1. Norepinephrine2. Epinephrine3. Normetanephrine4. Dopamine5. Metanephrine6. 3-Methoxy tyramineNegative Mode (ESI-)7. Vanillylmandelic acid (VMA)8. 5-Hydroxyindoleacetic acid (5-HIAA)9. Homovanillic acid (HVA)

Recommended Sample Preparation MethodProduct: Strata-X-CW Microelution 96-Well PlatePart No.: 8M-S035-4GA

Condition: 200 µL MethanolEquilibrate: 200 µL Water

Load Sample: 250 µL Plasma diluted with 250 µL WaterWash 1: 200 µL WaterWash 2: 200 µL 50:50 Acetonitrile/IPA

Dry for 1 minute at 10” Hg to remove excess wash solvent

Elute: 2 x 25 µL 85:15 Acetonitrile/Water with 2 % Formic acid


19

Polar Case Study – ETG/ETS

Ethyl glucuronide (EtG) and ethyl sulfate (EtS) are metabolites of ethanol that are used by analytical laboratories to detect recent ethanol ingestion. In this application, the Luna® Omega Polar C18 provides excellent separation between EtG and EtS, along with valuable separation from a urine interference peak and where other matrix suppression is common. Additionally, the high efficiency lev-els provided by the Luna Omega 1.6 µm allow for a highly sensitive, yet rapid method.ETG and ETS from Urine Sample

Ap

p ID

235

97A

pp

ID 2

3597

Ap

p ID

235

97

Inte

nsity

, cp

sIn

tens

ity, c

ps

Inte

nsity

, cp

s

2000400060008000

0

1.0e41.2e41.6e41.8e42.0e42.2e42.4e42.6e42.8e43.0e43.2e43.4e43.6e4

min2 31

2

3

1

200100

0

400300

600500

800700

9001000

12001100

13001365

2 3 min1

3

1


Dimension: 100 x 2.1 mmPart No.: 00D-4748-AN

Mobile Phase: A: 10 mM Ammonium Formate with 0.1 % Formic AcidB: Acetonitrile with 0.1 % Formic Acid


1.15

% B05000



1. Urine interference2. ETS3. ETG

Overlaid XIC

XIC to ETS

XIC to ETG

2000400060008000

0

1.0e41.2e41.6e41.8e42.0e42.2e42.4e42.6e42.8e43.0e43.2e43.4e43.6e4

min2 31

2

1

ETS

ETG


20

Complementary UHPLC Work Horses

Combine the versatile interaction mechanisms of the Luna® Omega C18 and Polar C18 to achieve successful separations and improve upon challenging ex-isting methods.

Natural Cannabinoids

Influence Selectivity with 100 % Aqueous Stability and Polar Interactions

Intact Peptides

Ap

p ID

235

81


Luna Omega 1.6 µm C18Dimension: 100 x 2.1 mm

Mobile Phase: A: 20 mM Ammonium Formate pH 3.2B: Acetonitrile


13.0115

% B6095956060


Detection: UV @ 256 nmSample: 1. CBVD

2. Cannabidiol 3. CBG 4. Cannabidolic Acid 5. CBG-A 6. Cannabinol 7. Delta 9 THC 8. Delta 8 THC 9. CBC 10. THCA-A

Conditions for all columns except where noted:Columns: Luna Omega 1.6 µm Polar C18

Luna Omega 1.6 µm C18Dimension: 50 x 2.1 mm

Mobile Phase: A: Water with 0.1 % TFAB: Acetonitrile with 0.1 % TFA


% B0065


Detection: UV @ 210 nmSample: 1. Gly-Tyr

2. Val-Tyr-Val3. Met-Enkephalin4. Leu-Enkephalin5. Angiotensin II

Ap

p ID

235

80

Inte

nsity

, cp

sIn

tens

ity, c

ps

0

2 3 4 5 min1

200

400

600

mAU

0

2 3 4 5 min1

200

400

600

mAU

Ap

p ID

236

06

Ap

p ID

236

09

0

4 6 7 8 9 105 min3

0.010

0.020

0.030

0.040

0.050

0.060

0.070mAU

0

4 6 7 8 9 105 min3

0.010

0.020

0.030

0.040

0.050

0.060

0.070mAU

Use the 100 % aqueous stability of polar C18 to aid in better retention of polar compounds


Greater hydrophobic retention






21

Complementary UHPLC Work Horses

For the analysis of a large screen or mixture of compounds, you can benefit greatly from utilizing both the Luna® Omega C18 and Polar C18 during method development. The strong focused hydrophobic retention of the C18 will provide excellent non-polar retention and separation, while the Polar C18 provides a complementary hydro-phobic/polar combined selectivity that can retain and separate typically early eluting compounds.

Tryptic Digest - Peptide Map of BSA

Conditions same for all columns:Columns: Luna Omega 1.6 µm C18

Luna Omega 1.6 µm Polar C18Dimension: 100 x 2.1 mm



50.1

% B3503


Detection: MS/MS (SCIEX API 4000™)Sample: Digested BSA

Inte

nsity

, cp

s

1.00e82.00e83.00e84.00e85.00e86.00e87.00e88.00e89.00e81.00e92.00e93.00e94.00e9

14 16 18 20 22 24 26 28 30 32 34 36 38 min2 4 6 8 10 12

Ap

p ID

235

99

Inte

nsity

, cp

s

0.001.00e82.00e83.00e84.00e85.00e86.00e87.00e88.00e89.00e81.00e91.10e9

10 20 30 min

Ap

p ID

236

00

Inte

nsity

, cp

s

Inte

nsity

, cp

s

018 19 min17

2.00e8

4.00e8

6.00e8

8.00e8

10.00e8

12.00e8

Ap

p ID

236

01

Ap

p ID

236

01


Luna Omega 1.6 µm

Polar C18



Greater resolution of early, more polar eluters

Better resolution of more hydrophic compounds


018 19 min17

2.00e8

4.00e8

6.00e8

8.00e8

10.00e8

12.00e8


22

Luna Omega + Kinetex = Happy UHPLC

Increase your method development options by screening a combination of core-shell and fully porous selectivities.

Natural Cannabinoids

Inte

nsity

, cp

sIn

tens

ity, c

ps

0

4 6 7

1 2 37 8

910

4

5

6

8 9 105 min3

0.010

0.020

0.030

0.040

0.050

0.060

0.070mAU

0.010

0.020

0.030

0.040

0.050

0.060

0.070

0

4 6 7 8 9 105 min3

mAU

178

9 10

2-4

5

6

Ap

p ID

235

82A

pp

ID 2

3580


Kinetex 1.7 µm BiphenylKinetex 1.7 µm F5

Dimension: 100 x 2.1 mmMobile Phase: A: 20 mM Ammonium Formate pH 3.2

B: AcetonitrileGradient: Time (min)

01213

13.0115

% B6095956060


Detection: UV @ 256 nmSample: 1. CBVD

2. Cannabidiol 3. CBG 4. Cannabidolic Acid 5. CBG-A 6. Cannabinol 7. Delta 9 THC 8. Delta 8 THC 9. CBC 10. THCA-A

Luna® Omega 1.6 µm Polar C18

Kinetex 1.7 µm Biphenyl

TMSTMS

PolarPolar

TMSTMS

Biphenyl

Inte

nsity

, cp

s

0

0.010

0.020

0.030

0.040

0.050

0.060

0.070

4 6 7 8 9 105 min3

mAU

1 2 3 7 8

910

4

5

6

Ap

p ID

235

83

Kinetex® 1.7 µm F5

F5

F

F

F

F

F

TMSTMS

Excellent resolution

Faster analysis with similar peak order

Alternative peak elution order



23

Conditions for all columns except where noted:Columns: Luna Omega 1.6 µm C18

Kinetex 1.7 µm BiphenylKinetex 1.7 µm EVO C18

Dimension: 50 x 2.1 mmMobile Phase: Luna Omega 1.6 µm C18 and Kinetex 1.7 µm Biphenyl

A: 0.1 % Formic Acid in WaterB: 0.1 % Formic Acid in AcetonitrileKinetex 1.7 µm EVO C18A: 10 mM Ammonium Bicarbonate pH 10B: Acetonitrile


5.1

% B595955


Detection: MS/MS (SCIEX API 4000™)Sample: Drugs of Abuse

Luna Omega + Kinetex =Happy UHPLC

Combining orthogonal UHPLC particle morphologies and selectivities like the Luna Omega C18, Kinetex Biphenyl, and Kinetex EVO C18 will drastically in-crease your probability of separation success!

Morphine

Drugs of Abuse Analysis Luna® Omega 1.6 µm C18

Kinetex® 1.7 µm Biphenyl

Kinetex 1.7 µm EVO C18A

pp

ID 2

3444

1 2 3 4 min

23443

1 2 3 4 min

1 2 3 4 min

Ap

p ID

234

43

pH 2.5

pH 2.5

pH 8.5

ADVANTAGE Excellent inertness and

strong hydrophobic retention

ADVANTAGE Extra aromatic (pi-pi)

and dipole-dipole retention

ADVANTAGE Excellent peak shape and

retention of bases at high pH

Ap

p ID

234

45



24

Excellent Lifetime

Luna Omega 1.6 µm media was designed to be durable and withstand the high pressures and rigors of UHPLC work in combination with providing excellent performance.

Accelerated Lifetime Study

% In

crea

se o

ver

Initi

al

Bac

kpre

ssur

e

Injection Cycle

Luna® Omega 1.6 µm C18 (AVG)

Waters® ACQUITY BEH 1.7 µm C18 (AVG)

0 10 20 30 40 50 600.0%

1.0%

2.0%

3.0%

4.0%

5.0%

6.0%

7.0%

8.0%

9.0%

10.0%

Ap

p ID

236

10

Conditions same for both columns:Columns: Luna Omega 1.6 µm C18




4.1

% B5955


Detection: UV @ 210 nmSample: Protein Matrix


Extend UHPLC Column Lifetime Even More with SecurityGuard ULTRASee page 26 for details


25

Further Improve Lifetime with Sample Preparation Both UHPLC instrumentation and columns can benefit from consistent removal of microparticulates/contaminants that may appear in solvents, samples or spe-cific matrices. Over time these problematic compounds can build up on instru-mentation and columns, leading to the need for costly maintenance and repair. Strata-X polymeric SPE, Novum liquid extraction, and Phenex syringe filters are excellent options to try.

Convenient and inexpensive way to remove particulates from LC samples

www.phenomenex.com/Phenex

novumsimplified liquid extraction

Polymeric SPE

Very targeted form of sample preparation that allows you to isolate your analyte of interest while removing any interfering compounds in your sample

www.phenomenex.com/strataX

Remove interferences such as proteins, phospholipids, salts, and more in a quick and simple procedure

www.phenomenex.com/Novum

Sample Preparation


26

Protect any UHPLC Column

Protect your UHPLC column, including Luna® Omega columns, from dam-aging contaminants and microparticulates with the SecurityGuard ULTRA guard cartridge system!

• Simple to use

• Extend column lifetime

• Pressure rated to 20,000 psi (1,378 bar)

• Fits virtually all manufacturers’ columns 2.1 to 4.6 mm ID

UHPLC Column Protection

See it in action: www.phenomenex.com/SecurityGuardULTRA

Secu

rityGu

ard™ U

LTR

AU

HP

LC C

olumn to D

etectorFrom

Injector

CartridgeHolder

SecurityGuard ULTRA Guard Cartridge System (Cartridge connected to Holder)

* Cartridge schematic not drawn to scale

Compounds of Interest

Contaminants and Particulates

Material Chamber

FritContaminants Trapped


27

Ordering Information

See it in action: www.phenomenex.com/SecurityGuardULTRA

Buy Luna® Omega Now

Ordering Information

Luna for HPLCDon’t miss out on classically wonderful Luna HPLC columns in a variety of selectivities and particle sizes:

2.5 µm, 3 µm, 5 µm, 10 µm C18(2), Si(2), C8(2), C5, Phenyl-Hexyl, PFP(2), HILIC, CN, NH2, SCX

Find them at: www.phenomenex.com/Luna


If Luna analytical columns do not provide at least an equivalent separation as compared to a competing column of the same particle size, similar phase and dimensions, return the column with comparative data within 45 days FOR A FULL REFUND.

1.6 μm Minibore Columns (mm)SecurityGuard™

ULTRA Cartridges‡

Phases 30 x 2.1 50 x 2.1 100 x 2.1 150 x 2.1 3/pk

Polar C18 00A-4748-AN 00B-4748-AN 00D-4748-AN 00F-4748-AN AJ0-9505C18 00A-4742-AN 00B-4742-AN 00D-4742-AN 00F-4742-AN AJ0-9502

for 2.1 mm ID‡ SecurityGuard ULTRA Cartridges require holder, Part No.: AJ0-9000

28

www.phenomenex.comPhenomenex products are available worldwide. For the distributor in your country, contact Phenomenex USA, International Department at [email protected]

Australiat: +61 (0)2-9428-6444 f: +61 (0)2-9428-6445

[email protected]

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All other countries

Corporate Office USA t: +1 (310) 212-0555f: +1 (310) 328-7768

[email protected]

Terms and Conditions Subject to Phenomenex Standard Terms & Conditions, which may be viewed at www.phenomenex.com/TermsAndConditions.Trademarks Luna, Kinetex, and Strata are registered trademarks and SecurityGuard, Novum, and Phenex are trademarks of Phenomenex. ACE is a registered trademark and Excel is a trademark of Advanced Chromatography Technologies Limited. Waters, ACQUITY, UPLC, and CORTECS are registered trademarks of Waters Corporation. Agilent and ZORBAX are registered trademarks of Agilent Technologies, Inc. Thermo Scientific is a registered trademark and Syncronis is a trademark of Thermo Fisher Scientific. Hypersil GOLD is a registered trademark of Thermo Hypersil-Keystone LLC. AkzoNobel and Kromasil are registered trademarks of AkzoNobel Pulp and Performance Chemicals AB. YMC is a registered trademark of YMC Co., Ltd. API 4000 is a trademark of AB SCIEX Pte. Ltd. AB SCIEX™ is being used under license.Disclaimer Phenomenex is not affiliated with Advanced Chromatography Technologies Limited, Agilent Technologies, AkzoNobel, Thermo Fisher Scientific, YMC, or Waters Corporation.Comparative separations may not be representative of all applications.© 2016 Phenomenex, Inc. All rights reserved.B

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