HPLC/UHPLC DetectorsCLARIT Y W ITHOUT COMPROMISE

Liquid chromatography is one of the most important tools in an analytical laboratory, it is often referred to as the gold standard for quantification. We offer the tools for your business to deliver unequivocal results that provide you with confidence in your scientific and business decisions.

Our wide complement of detectors give you the technology to fit within your existing laboratory workflows without compromising the quality of the data or the timeliness of your results.

Our HPLC/UHPLC detectors grant you flexibility for analyzing those samples with ease, no matter the type of analysis you are performing.

ENSURE YOU GET THE ANSW ERS TO THE QUEST IONS YOU ARE ASKING

■■ Optional flow cells to match the scale of your LC needs■■ Compatibility with Empower® and MassLynx® software■■ Compatibility with Alliance® HPLC and the ACQUITY® Arc™ systems

Every lab faces a different set of challenges and problems. In order to successfully meet those challenges, it is important to have the right tools available in your toolbox.

Whether you routinely develop new methods or perform routine analysis on your samples, our HPLC/UHPLC detectors can fit into your systems to help you reach your laboratory’s goals of decreasing the time to result, while increasing the depth of the quality of information about your samples.

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Flow rate: 1.4 mL/minColumn: XBridge® C18, 3.5 μm, 4.6 x 100 mmSample: 4 μg/mL phenone mixture

The capabilities and performance of Waters Detectors provides flexibility to meet the sensitivity needs of HPLC and UHPLC methods.

FLEXIBILIT Y AND COMPATIBILIT Y TO MEET ALL YOUR HPLC/UHPLC DET ECT ION NEEDS

WIDE RANGE OF DETECTION TECHNIQUESThe 2465 Electrochemical and the 2432 Conductivity detectors help with challenging separations that require a specialized detection technique.

ADAPTABLE ANALYSIS SETTINGSWaters detectors provide flexibility to meet your existing HPLC methods, as well as the more challenging requirements found in your UHPLC methods, providing you the ability to future-proof your labs.

DATA CLARITYUser settings allow optimizing the detectors for the sensitivity and resolution needed for your analysis.

SUPERIOR LINEAR RANGEThe wide linear range of the detectors provide the tools needed to meet the most challenging limits of detection.

COMPLEMENTARY WITH MASS DETECTIONWaters detectors are complimentary. The ACQUITY® QDa® Mass Detector ensures confidence in your LC analysis by providing mass confirmation of all peaks in your separation.

DETECTOR OPTIONS TO MEET YOUR NEEDSA wide range of options are available for Waters® detectors to provide the versatility and flexibility to meet a laboratory’s analysis.

IMPROV ED SAMPLE CHARACT ERIZATION

The 2998 Photodiode Array (PDA) Detector uniquely integrates software and optics innovation to deliver no-compromise chromatographic and spectral sensitivity. The exceptional signal-to-noise ratios, high-sensitivity library matching, and high optical and digital resolution enables the 2998 PDA Detector to map low levels of compounds and determine trace impurity levels within a compound’s peak. Additionally, it has a wide linear dynamic range that allows for simultaneous quantification of high and low level components within a single chromatographic separation.

The 2998 PDA Detector is reliable, easy-to-use and has enhanced software control to provide flexibility for simultaneous 2D and 3D operation with either Empower or MassLynx Software.

While the design of most PDA detectors can only distinguish between compounds possessing comparatively large spectral differences, the 2998 PDA Detector can differentiate between the spectra of closely-related compounds. When combined with the ACQUITY QDa Mass Detector, you now have information rich mass spectral data to complement your current Waters PDA, UV/Vis, ELS, and Conductivity Detector that gives you a more complete separation.

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ACQUITY QDa:Mode: ESI+Mass range: 100–450Sampling rate: 2 Hz

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Spectral analysis algorithms can distinguish closely related compounds, as shown

in this Clozapine and related impurities example, providing reliable library matching.

Use of the 2998 PDA Detector with the ACQUITY QDa allows for confidence in information about your sample, by providing mass confirmation of each peak, in example: Fluconazole USP Method.

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SENSIT IV IT Y AND SELECT IV IT Y

The 2489 UV/Visible (UV/Vis) Detector is an ultraviolet/visible detector that provides superior sensitivity and linear dynamic range which are needed for the detection of even the lowest-level impurities. The design for the most sensitive and versatile dual wavelength absorbance detector combines a high energy Deuterium source, a precise optical design, and low noise, high speed electronics to ensure performance, reliability and usability.

The 2475 Fluorescence (FLR) Detector offers unsurpassed flexibility whether you are doing routine analysis or method development. It is the ideal tool for high sensitivity methods due to its low limits of repeatable quantification coupled with its high selectivity for analytes in complex matrices. Waters unique axially illuminated flow cell minimizes unwanted light scatter and dispersion compared to a traditional cubic flow cell. The 2475’s unique calibration procedure for the photomultiplier tube and the ability to report detector signal in emission units (EU) help reduce the variability traditionally observed from detector to detector.

The complementary nature of our HPLC/UHPLC Detectors provides you with all the tools needed to ensure the right answers to your analysis.

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System: Alliance HPLC with 2489 UV/Vis DetectorSample: 1.0 mg/mL Irbesartan tabletColumn: XSelect™ HSS T3, 4.6 mm x 250 mm, 5 µmInjection vol.: 8 µL Flow rate: 1.0 mL/minDetection: 220 nm, 40 Hz, 0.05 s (normal)

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Impurity 1 0.006 53Impurity 2 0.011 70Impurity 2 0.034 213Impurity 2 0.003 18RCA 0.018 89Irbesartan tablet 99.927 422602

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The 2489 UV/Vis Detector provides excellent signal-to-noise response for methods which require low levels of sensitivity, such as found in this example of Irbesartan and its related impurities.

The use of the 2475 Fluorescence Detector allows for selectivity and sensitivity of compounds. In this example, beta blockers have no UV response, making the 2475 the perfect detector choice for analysis of the sample.

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THE RIGHT TOOL FOR THE RIGHT JOB

Laboratories around the world continue to rely on HPLC to properly quantify their samples. When combining the Alliance HPLC or the ACQUITY Arc systems with the 2424 Evaporative Light Scattering (ELS) and the 2414 Refractive Index (RI) detectors, we provide users the benefit of detecting compounds that have poor to no UV/Vis response or analytes that do not ionize well under mass spectrometry.

The 2414 RI Detector provides the highest sensitivity, stability and reproducibility of any RI detector on the market. Waters innovative counter-current heat exchanger quickly equilibrates temperature differences between the solvent stream entering and exiting the flow cell. The unique temperature control prevents fluctuations in viscosity and refractive index to allowing for repeatable measurements even at lower concentrations. The 2414 RI Detector completes a total sample-centric solution for a wide variety of analyses.

The 2424 ELS Detector balanced design delivers reproducible quantification for semi-volatile components without adding high dispersion to fast LC separations. It has been designed with active nebulizer heating and cooling, auto-optimize gain function and optional external column heater control to maximize your method performance. The detector’s compatibility with gradient analysis allows for a wider separation range of your samples.

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Apple Juice

Of the five recognized food sugars (fructose, glucose, sucrose, maltose, and lactose), the first three are the most important in fruit juice analysis. These sugars are quickly and easily analyzed on a system utilizing the 2414 RI Detector, the Alliance HPLC System, and an XBridge® Amide XP Column.

The gradient analysis of triglycerides in edible oils, overlay of safflower oil (blue) and olive oil (green) using the 2424 ELS Detector.

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FOR YOUR MOST CHALLENGING SEPARATIONS

The 2465 Electrochemical (EC) Detector combines sensitivity, reliability, and simplicity to deliver superior electrochemical detection performance. The 2465 EC Detector provides a wide range of working and reference electrodes in a rugged flow cell design which can be used in multiple detection modes – direct current, pulsed amperometric and scanning – that delivers a fully automated, reliable electrochemical system that provides detection sensitivity suitable for a wide variety of application needs.

The 2432 Conductivity Detector features exceptional sensitivity and stability for single-column or suppressor-based ion chromatography. The 2432 cell design diminished apparent void volume disturbances for more reliable peak quantitation. Combining the 2432 Conductivity Detector’s temperature control and autozero capabilities you can be assured of maintained baseline stability during your analysis.

The 2432 Conductivity Detector.The 2465 EC Detector.

Waters, The Science of What’s Possible, Empower, MassLynx, Alliance, ACQUITY, UPLC, XBridge, and QDa are registered trademarks of Waters Corporation. Arc and XSelect are trademarks of Waters Corporation. All other trademarks are the property of their respective owners.

©2016 Waters Corporation. Printed in the U.S.A. April 2016 720005629EN LM-KP

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