Post-transcriptional control of BDNF mRNA homeostasis and ...
3
Post-transcriptional control of BDNF mRNA homeostasis and translation through the distinct 3’UTRS Glioma cell lines 255 0 Ctr Synaptic activation 0 1 2 3 4 5 6 7 8 9 10 0 10 20 30 40 50 60 * * Fraction number Percent total Activity-dependent BDNF translation polyribosome fractions BDNF 3’UTR reporter Hippocampal BDNF protein ctr seizure Lau et al., PNAS, 107(36):15945; Allen M et al., PLoS One 8(1):e55718
Transcript of Post-transcriptional control of BDNF mRNA homeostasis and ...
Post-transcriptional control of BDNF mRNA homeostasis and translation through the distinct 3’UTRS
Glioma cell lines
255
0
Ctr
Synaptic activation
0 1 2 3 4 5 6 7 8 9 100
10
20
30
40
50
60ControlPilo-30min
**
Fraction number
Percent total
Activity-dependent BDNF translation
polyribosome fractions
BDNF 3’UTRreporter
Hippocampal BDNF protein
ctrseizure
Lau et al., PNAS, 107(36):15945; Allen M et al., PLoS One 8(1):e55718
Cdk5 pathway in postnatal brain development and diseases
Li et al., J Neurosci 36:11283;; Tan et al., Neuroscience 290:552;; Bankston et al., J Biol Chem. 288:18047
3’UTR
5’cap
FMRP
FMRP targetmRNA
The fragile X mental retardation protein (FMRP) cooperates with microRNA to govern translation, neural
network development and synaptic function
Ctr Fmr1 siRNA0
50
100
150
200
miRNA
WT
Fmr1 KO
B
Axonal projectionSynaptic FMRP targets
Erk1/2
PI3K
FMRP
S6K mediated translation
Translation of direct FMRP target mRNAs
FMRP suppresses basal protein synthesis and enables synaptic activity-dependent translation
