CARBOPEPTIDASE E SPLICE VARIANT PREDICTS METASTASES.

ANDGC CONTENT AROUND SPLICE SITES AFFECTS SPLICING THROUGH PRE-mRNA SECONDARY

STRUCTURES.

BY: ALEJANDRA ALVAREZ

STUDENT OF MEDICINE

INTRODUCCION

Protein synthesis guarantees us thedevelopment, proliferation and cellular activity. forthis is that the splicing is relevant in this process

Hepatocellular carcinoma is the cause of death of manypeople in the world. It is caused by alcoholconsumption, inflammation of the liver, among othercauses. Through the research conducted in this diseasefailed to find a biomarker that indicates thepresence and development of this cancer.

CARBOPEPTIDASE E ESPLICE VARIANT PREDICTS METASTASES

from the University of Hong Kong, analyzed 99 Hepatocellular carcinoma patients and quantified the amount of Carbopeptidase E to determine the ratio of tumor to normal tissue.

The investigators found that in 90 percent of Hepatocellular carcinoma patients, increase the Carbopeptidase E in primary tumor compared to surrounding normal tissue predicted that the cancer was going to return or metastasize in two years.

The prediction was

independent of cancer stage

assay provides a major breakthrough in biomarker discovery with invaluable utility in cancer prognosis for predicting future metastasis and recurrence in patients with Hepatocellular carcinoma, and for other types of cancer as well.

STUDENT OBSERVATION

This new is very important for the scientific community because it is used to search for new markers that indicate the presence of a disease like cancer and it also especificate how bad it is.

GC CONTENT AROUND SPLICE SITES AFFECTS SPLICING THROUGH PRE-mRNA SECONDARY STRUCTURES.

Alternative splicing increases protein diversity by generating multiple transcript isoformsfrom a single gene through different combinations of exons or through different selections of splice sites

Here we perform a genomic study of RNA secondary structures around splice sites in humans (Homo sapiens), mice (Musmusculus), fruit flies (Drosophila melanogaster), and nematodes

We observe that GC content around splice sites is closely associated with the splice site usage in multiple species

RNA secondary structure is the possible explanation, because the structural stability difference among alternative splice sites, constitutive splice sites, and skipped splice sites can be explained by the GC content difference

We also found that the GC-content effect is much stronger than the nucleotide-order effect to form stable secondary structures

STUDENT OBSERVATION

This research allows thedevelopment of knowledgeabout the synthesis of proteins in all cellularprocesses and can be usedfor the development of knowledge of DNA.

.

MEDICAL UTIILITY

This allows to promote the research in different fields of medicine.

In the first case can be found as is possible new markers that enable the discovery of carcinomas of various types

In the first case can be found as is possible new markers that enable the discovery of carcinomas of various types, In the first case can be found as is possible new markers that enable the discovery of carcinomas of various types, In the first case can be found as is possible new markers that enable the discovery of carcinomas of various types, In the first case can be found as is possible new markers that enable the discovery of carcinomas of various types, In the first case can be found as is possible new markers that enable the discovery of carcinomas of various types, In the first case can be found as is possible new markers that enable the discovery of carcinomas of various types, In the first case can be found as is possible new markers that enable the discovery of carcinomas of various types, In the first case can be found as is possible new markers that enable the discovery of carcinomas of various types,

in the second case shows the great importance of the processes occurring at the cellular level.

in the second case shows the great importance of the processes occurring at the cellular level.

This show that peoplewants to improve thetecnology to help thedoctor to make a betterdiagnosis

MARTINEZ S, Lina María. Biología molecular. 6. ed. Medellín: UPB. Fac. de

Medicina.

· Carboxypeptidase E SpliceVariant Predicts MetastasesJournal of Clinical Invest.

· GC content around splice sites affectssplicing through pre-mRNA secondaryStructures.

BILIOGRAPHY