MicroRNA-613 represses lipogenesis in HepG2 cellsby downregulating LXRalpha

ANDThe specifity of urinary aquaporin 1 and perilipin 2

to screen for renal cell carcinoma

MATEO ZAPATA NARANJO

UNIVERSIDAD PONTIFICIA BOLIVARIANA

INTRODUCTION

In these times when medicine has been evolving and every day shows the importance of research, molecular biology techniques and especially their transfer of genetic material have reached places as accurate and enabled evaluate everything. Most of the techniques used have shown an essence on the basics, as many people had forgotten that today are solving the major problems of medicine

FIRST NEW

MicroRNA-613 represseslipogenesis in HepG2 cells by

downregulating LXRalpha

The main objective was to obtain a technique which could regulate lipid homeostasis by expression of certain genes associated with specific receptors tied to microRNA's

FIRST NEW

The report showed that the microRNA miR-613 manages generally negatively regulate the liver X receptor alpha, which is activated by a ligandand is vital in the metabolism of lipids

FIRST NEW

This was performed in HepG2 cells, and all was performed by real-time PCR, western blot and luciferase. It was possible to demonstrate that miR-613 induces formation of lipid droplets in HepG2 cells and thereby down-regulating receptor expression LXRalpha

FIRST NEW

In conclusion, was developed and obtained a technique which is achieved by mediating the metabolism of lipids, which is very important from now on to treat all kinds of diseases covering these compounds

FIRST NEW

My personal opinion on this article is that it is very important what has been developed. Given the conditions that cause lipid disorders, will be very important in some way to correct these issues in a manner based on the molecular. Each day shows the importance of molecular biology major alternatives pathologies.

SECOND NEW

The specifity of urinaryaquaporin 1 and perilipin 2 toscreen for renal cell carcinoma

This new shows us the importance of diagnosing quickly and easily renal carcinoma. Have determined concentrations of certain metabolites in urine that have come to be associated with this disease.

SECOND NEW

Rapid diagnosis of renal cell carcinoma has not been established by simple techniques. By AQP1 concentrations and PLIN2 that serve as biomarkers in urine tests generate a light that is associated with a rapid and simple diagnostic allowing adequate and timely treatment

SECOND NEW

To reach such conclusions were made several tests in people with renal carcinoma and several similar urinary infections. AQP1 levels in urine and PLIN2 were determined by Western blotting. Very high concentrations were found in patients suffering from renal carcinoma compared with other diseases

SECOND NEW

In conclusion, the findings of these metabolites help diagnose renal carcinoma and differentiate it from other diseases that can cover something more serious such as carcinoma. There will be further assessed more results and comparing them to establish the rapid diagnosis of renal carcinoma.

SECOND NEW

In my opinion the development of the mentioned mechanism is quite important in the search for a diagnosis quickly above that allows the doctor to easily interpret and act in an appropriate manner. It was a first step but I think quite appropriate to do a study of more participants who are able to unify the concept being treated and reach what has been proposed

MEDICAL UTILITY

First New

The main medical benefit of this news is in force that have taken the disease etiology in lipid disorders and as mentioned technique THROUGH modulate the effects are achieved lipid own. It is important today for the diet that currently contains the general population to modify lipid metabolism and improve many boxes each day disease claims the lives of many people.

MEDICAL UTILITY

It is a situation, is a public health problem that is truly useful to develop this technique.

Molecular biology greatly aid and generates large amounts of expectations for development that can result I think will be pretty good

MEDICAL UTILITY

Second New

The main use of this article focuses on the speed and ease with which the doctor can diagnose a renal carcinoma compared with other procedures that generally do not allow the diagnosis or that take too long to adapt clinical diagnosis for the real.

MEDICAL UTILITY

It is useful if this is accomplished medical check since it would be a breakthrough in issues like this that have lately been so unemployed and revolutionize the approach to a simple urinary tract infection.

The great use is accompanied by the ease and results are obtained using a urine test that is fairly common and that will bring the doctor to the pathology

BIBLIOGRAPHY

• Martinez S. Lina María. Biología Molecular, 5 ed. Medellín, UPB, Facultad de Medicina

• MicroRNA-613 represses lipogenesis in HepG2 cells bydownregulating LXRalpha, 7th Space interactive, March8/2013,http://7thspace.com/headlines/433798/microrna_613_represses_lipogenesis_in_hepg2_cells_by_downregulating_lxralpha.html

• The specifity of urinary aquaporin 1 and perilipin 2 to screenfor renal cell carcinoma, Uro Today, March 11/2013

http://www.urotoday.com/Renal-Cancer/the-specificity-of-urinary-aquaporin-1-and-perilipin-2-to-screen-for-renal-cell-carcinoma-abstract.html