PARTICIPANTS AT THE MEETING

A.A. ABDEL-LATIF - Department of Cell and Molecular Biolo­gy, Medical College of Georgia, Augusta, Georgia 30902, U.S.A.

D. ALLAN - Department of Biochemistry, University of Bir­mingham, P.O. Box 363, Birmingham B15 2TT, Fhgland.

L. AMADUCCI - Department of Neurology, University of Flo­rence, Florence, Italy.

G.B. ANSELL - Department of Phannacology, The Medical School, Birmingham B15 2Tj, England.

G. ARIENTI - Department of Biochemistry, The Medical School, P.O. Box 3, University of Perugia, Perugia, Italy.

L. AUSTIN - Department of Biochemistry, Monash University, Clayton, Victoria, 3168, Australia.

G.F. AZZONE - C.N.R. Unit for the Study of Physiology of Mitochondria and Institute of General Pathology, University of Padua, Padua, Italy.

L. BARTOLINI - Department of Neurology, University of Flo­rence, Florence, Italy.

N.G. BAzAN - Instituto de Investigaciones Bioquimicas, Universidad Nacional del Sur, Bahla Blanca, Argentina. ,

D. BENVEGNU - F.I.D.I.A. Research Laboratories, Abano Ter-me, Padua, Italy.

L. BINAGLIA - Institut fUr Physiologische Chemie der Uni­versit[t K6ln, D-5 K6ln 41, joseph Stelzmann Strasse 52, West Germany.

j.E. BLEASDALE - Department of Biochemistry, University Hospital and Medical School, Nottingham NG7 2UH, England.

E. BOARATO - F.I.D.I.A. Research Laboratories, Abano Terme, Padua, Italy.

M. BRAGADIN - C.N.R. Unit for the Study of Physiology of Mitochondria and Institute of General Pathology, University of Padua, Padua, Italy.

M. BRUNETTI - Department of Biochemistry, The Medical School, P.O. Box 3, University of Perugia, Perugia, Italy.

373

374 PARTICIPANTS

A. BRUNI - Department of Pharmacology, University of Padua, Largo E. Meneghetti 2, 35100 Padua, Italy.

R.l. CENEDELLA - Department of Pharmacology, West Virginia University, Morgantown, West Virginia 26506, U.S.A.

F. CERRITO - Department of Pharmacology, University "S. Cuore", Rome, Italy.

1.Y. CHIN - Department of Physiological Chemistry, The Ohio State University, Columbus, Ohio 43210, U.S.A.

F. CLEMENTI - Department of Pharmacology, The Medical School, University of Milan, Milan, Italy.

M. COMPORTI - Department of General Pathology, The Univer­sity of Siena, Siena, Italy.

R.M.C. DAWSON - Biochemistry Department, A.R.C. Institute of Animal Physiology, Babraham, Cambridge CB2 4AT, England.

F. DELLA VALLE - F.I.D.I.A. Research Laboratories, Abano Terme, Padua, Italy.

G.E. DE MEDIO - Department of Biochemistry, The Medical School, P.O Box 3, University of Perugia, Perugia, Italy.

1. EICHBERG - Department of Biophysical Sciences, Univer­sity of Houston, Houston, Texas 77004, U.S.A.

1.B. FINEAN - Department of Biochemistry, University of Birmingham, P.O. Box 363, Birmingham B15 2TT, England.

S. FLEISCHER - Department of Molecular Biology, Vanderbilt University, Nashville, 37235 Tennessee, U.S.A.

E. FRANCESCANGELI - Department of Biochemistry, The Medical School, P.O. Box 3, University of Perugia, Perugia, Italy.

A. GAITI - Department of Biochemistry, The Medical School, P.O. Box 3, University of Perugia, Perugia, Italy.

C. GALLI - Department of Pharmacology and Pharmacognosy, University of Milan, 20129 Milan, Italy.

C. GIAMBALVO - Neurology Research, The Children's Hospital Medical Centre, Boston, Mass., U.S.A.

N.M. GIUSTO - Instituto de Investigaciones Bioquimicas, Universidad Nacional del Sur, Bahia Blanca, Argentina.

P. GONZATO - F.I.D.I.A. Research Laboratories, Abano Terme, Padua, Italy.

G. GORACCI - Department of Biochemistry, The Medical School, P.O. Box 3, University of Perugia, Perugia, Italy.

R.M. GOULD - Neurochemistry Department, Institute for Basic Research in Mental Retardation, 1050 Forest Hill Road, Staten Island 10314, New York, U.S.A.

R.l. GULLIS- Department of Biochemistry, University of Birmingham, P.O. Box 363, Birmingham B15 2TT, England.

PARTICIPANTS 375

G. HAUSER - Research Laboratory, McLean Hospital, Belmont, Mass. 02178, U.S.A.

J.N. HAWTHORNE - Department of Biochemistry, University Hospital and Medical School, Nottingham NG7 2UH, England.

L.A. HORROCKS - Department of Physiological Chemistry, The Ohio State University, Columbus, Ohio 43210, U.S.A.

S. HUTERER - Department of Neurology and the Institute of Medical Science, University of Toronto, Toronto, Canada.

M.G. ILINCHETA DE BOSCHERO - Instituto de Investigaciones Bioqufmicas, Universidad Nacional del Sur, Bahia Blanca, Argentina.

D. INZITARI - Department of Neurology, University of Flo­rence, Florence, Italy.

E.G. LAPETINA - Instituto de Biolog1a Celular, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires 53, Argen­tina.

A. LEON - F.I.D.I.A. Research Laboratories, Abano Terme, Padua, Italy.

G. LENAZ - Department of Biochemistry, The Medical School, University of Ancona, Ancona, Italy.

K. KARAGEOSIAN - Armenian Academy of Sciences, Erewan, U.S.S.R.

A.D. KUFFER - Department of Biochemistry, Monash Universi­ty, Clayton, Victoria, 3168, Australia.

C.T. KWOK - Deparment of Biochemistry, Monash University, Clayton, Victoria, 3168, Australia.

P. MANTOVANI - Department of Pharmacology, The Medical School, University of Florence, Florence, Italy.

R.H. MICHELL - Department of Biochemistry, University of Birmingham, P.O. Box 363, Birmingham B15 2TT, England.

W. J. MOORE - School of Chemistry, University of Sydney, N.S.W., Australia.

R. MOZZI - Department of Biochemistry, The Medical School, p.O. Box 3, University of Perugia, Perugia, Italy.

p. ORLANDO - Department of Pharmacology, University "S. Cuore", Rome, Italy.

H.E. PASCAUL DE BAzAN - Instituto de Investigaciones Bio­qufmicas, Universidad Nacional del Sur, Bahia Blanca, Argentina.

G. PEPEU - Department of Pharmacology, The Medical School, University of Florence, Florence, Italy.

M. PHILIPPART - U.C.L.A. Department of Psychiatry, 760 Westwood Plaza, Los Angeles,90024 California, U.S.A.

376 PARTICIPANTS

M.R. PICKARD - Department of Biochemistry, University Hospital and Medical School, Notthingham NG7 2UH, England.

G. PORCELLATI - Department of Biochemistry, The Medical School, P.O.Box 3, University of Perugia, Perugia, Italy.

H. RAHMANN - Abteilung Allgemeine and spezielle zoologie, 7000 Stuttgart 70, Hohenheim, Germany.

M. RODRIGUEZ - Escuela Bioquimica Farmaceutica, Facultad Ciencias, Universidad de Habana, Cuba.

PH. ROSENBERG - Section of Pharmacology and Toxicology, University of Connecticut, Storrs, Connecticut 06268, U.S.A.

C.E. ROWE - Department of Biochemistry, University of Birmingham, P.O. Box 363, Birmingham B15 2TT, England.

B. SHAPIRO - Department of Biochemistry, The Hebrew Uni­versity, Hadassah Medical School, Jerusalem, Israel.

C. SPAGNUOLO - Department of Pharmacology and Pharmaco­gnosy, University of Milan, 20129 Milan, Italy.

S. SPANNER - Department of Pharmacology, The Medical School, Birmingham B15 2TJ, England.

W. STOFFEL - Institut fUr Physiologische Chemie der Uni­versit[t KBln, D-5 KBln 41, Joseph Stelzmann Strasse 52, West Germany.

E. STRINNA - C.N.R. Unit for the Study of Physiology of Mitochondria and Institute of General Pathology, University of Padua, Padua, Italy.

A.Y. SUN - Sinclair Comparative Medicine Research Farm, University of Missouri, Columbia, Missouri 65201, U.S.A.

G.Y. SUN - Sinclair Comparative Medicine Research Farm, University of Missouri, Columbia, Missouri 65201, U.S.A.

B.Y. TANG - Department of Biochemistry, Monash University, Clayton, Victoria, 3168, Australia.

D.K. THOMPSON - Department of Physiological Chemistry, The Ohio State University, Columbus, Ohio 43210, U.S.A.

W. THOMPSON - Department of Biochemistry, University of Toronto, Toronto, M5S 1A8, Canada.

A.D. TOEWS - Department of Physiological Chemistry, The Ohio State University, Columbus, Ohio 43210, U.S.A.

G. TOFFANO - F.I.D.I.A. Research Laboratories, Abano Terme, Padua, Italy.

E. VIOLA - C.N.R. Unit for the Study of Physiology of Mi­tochondria and Institute of General Pathology, University of Padua, Padua, Italy.

PARTICIPANTS 377

J.R. WHERRETT - Department of Neurology and the Institute of Medical Science, University of Toronto, Toronto, Canada.

H. WOELK - Neurochemische Abteilung, Universit~ts-Nerven klinik, Homburg/Saar, West Germany. • -

E. YAVIN - Weizmann Institute of Science, Neurobiology Unit, Rehovot, Israel.

L.M. ZIEHER - Instituto de Biolog{a Celular, Facultad de Medicina, Uniyersidad de Buenos Aires, Buenos Aires 53, Argentina.

SUBJECT INDEX

Acetylcholine and phosphatidic acid breakdown in synaptosomes, 232 and "phospholipid effect" in iris muscle after ganglionecto­my, 245,248

and "phospholipid effect" in pancreas, 232 decrease of 32pi incorporation into acidic phospholipids in iris muscle after ganglionectomy, by, 249

effect on phosphatidylinositol and phosphatidate metab6lism, 207

effects on 32Pi incorporation into phospholipids of iris mu-scle, 227,229,249

output from cerebral cortex, 285 pool of, in brain, 162 stimulation of cyclic-GMP formation, by, 217 stimulation of phospholipase A2 in synaptic membranes,212,227 stimulation of 32pi incorporation into phospholipids of cor-tex slices, 227

Acetylcholinesterase in cerebellum, 165 in cortex,16S in synaptosomal plasma membranes, 170

Acridine orange response and phospholipid hydrolysis, 16

Acylation of choline glycerophospholipid and cyclic-AMP, 220 of choline glycerophospholipid, cyclic-AMP-mediated, and

Ca2+, 220 processes in synaptosomes and phospholipase A2, 193 stimulation of, by NaF, 219

Acylation-deacylation cycle in synaptosomes, and neurotransmitters, 194

Acyl-CoA formation, and noradrenaline, 222 formation, and cyclic-AMP, 222

379

380 SUBJECT INDEX

Acyl-CoA: lysophospholipid acyl transferase, stimulation of, 222

Acyl groups (see also fatty acids) modification by diets, 169,179 phosphatase activity on, 174 recycling of, 186 (n~9) in essential fatty acid deficiency, 187 turnover in membrane phosphoglycerides, 169,185,186

Acyl transferases effect of ethanol on, 191

Adenyl cyclase activation by phospholipids, 93 changes after brain cortex phospholipid administration, 308 dopamine-sensitive, 6,143 effect of propranolol and phentolamine on, 143

Adrenalin effect on brain glucose, 272,273

Adrenergic agonists and antagonists, effect on labelling of phospholipid in iris muscle, 231

~ blockers, stimulation of 32pi incorporation in pineal pho­spholipids, 237

blocking agents and 32pi incorporation into iris muscle pho­spholipids, 227,230

a receptors and "phospholipid effect" in iris muscle, 230 in rat brain, 230 in rat parotid, 230 in rat vas deferens, 230

stimulation and generation of 1,2-diacylglycerol, 10 Adrenoreceptors

antagonists, effect on lipid metabolism in retina, 143,145 antagonists, effect on phosphatidate phosphohydrolase, 146 effect on phosphatidylinositol turnover, 258 effect on phospholipid metabolism in the pineal gland, 149

Ageing effect on "phospholipid effect" in iris muscle, 234

Alcohol (see also ethanol) effect on membranes, 175

Alkenylacyl-glycerophosphoryl-ethanolamine in myelin, 172 in synaptosomal plasma membranes, 172

SUBJECT INDEX

Alkylacyl-GPE and ethanolaminephosphotransferase, 38 conversion to plasmalogen in neurinoma, 134 from alkylacyl glycerols, 38 synthesis by base-exchange, 67 to alkenylacyl-GPE, 38

Aminoacids in brain cortex phospholipid mixture, 274

Amobarbital effect on brain glucose, 273

Amphetamine effect on brain glucose,273

Anaesthetics and phosphatidylinositol labelling, 5

Anionic lipids and cationic drugs, 6

Arachidonic acid after electroshock, 317-335 in nervous system during early ischemia, 317-335 intracerebral injection, 186 labelled, incorporation in diacyl-GPI, 186,190 release during ischemia, 337,339,343,344

Aspartic acid stimulation of cyclic-GMP formation, by, 217 stimulation of phospholipase A2 in synaptic membranes, by,

212 Aspirine

on free arachidonate production in brain, 322 Atherogenic diet

ATP effect on brain phospholipids, 294

and cyclic-AMP-stimulated hydrolysis of phosphatidylcholine, 219

+ + ATPase (see also Na /K ATPase) and incorporation of oleate in membrane phospholipids, 216 and membrane polarization, 174 changes in brain of atherogenic-diet-fed animals, 296 effect of fatty acid deficiency on, 179 inactivation by lipoxydation of polyunsaturated fatty aCids,

178 in plasma membrane,4,6 in synaptosomes, 169,174 role of phospholipids on, 169,174

381

382

ATP-ADP exchange and ATPase, 174

ATP-depleted cells, 7

SUBJECT INDEX

Atropine 32 effect on Pi incorporation into iris muscle phospholipids, 229

Autoradiography electron microscopic autoradiography of nerve, 105,109 in the study of phospholipid exchange, 95-96,101 of sciatic nerve, 104 Schwann cells, in, 105

Axolemma changes in, 21

Axoplasmic transport of lipids, 101

Bacillus cereus phospholipase C from, 15

Base-exchange mechanism and choline incorporation into phosphatidylcholine, 164 in membranes, 164 reaction in iris muscle, 239

effect of DL-propranolol on, 237,239 effect of Ca2+ on, 239

Base~exchange reaction in phospholipid metabolism homologous and heterologous, 68,69 in brain subcellular fractions, 66 in cultured brain cells, 118,121 in neurons and glia, 66 in synaptosomes, 66 occurrence in vivo, possible functions, presence in brain, relation with Ca2+, relation with pH, 65

Behavioural changes

73-76 73,76 66,118 65

in atherogenic diet fed-animals, 301 in atherogenic diet fed-animals treated with phospholipids,

301 Bovine brain cortex phospholipid mixture

composition, 274 effect on acetylcholine output from cerebral cortex,286,287

SUBJECT INDEX 383

Brain

effect on atherogenic diet-fed animals, 295 effect on brain free glucose, 272

cultured cells, 115-122 incorporation of choline, ethanolamine and serine into lipids of cultured cells, 119-122

phospholipid, specific activity in dystrophic mice, 369

affinity with phosphatidylserine, 291 and elevation of cyclic-GMP, 235 and exocytosis, 235 and [+ efflux from parotid, 235 and "phospholipid effect" in rat diaphragms, 235 and "phospholipid effect" in the thyroid, 235 and stimulation of phospholipase C, 8 effect on cyclic-AMP-stimulated acylation of choline glyce­rophospholipid, 220

effect on cyclic-AMP-stimulated hydrolysis of phosphatidyl­choline, 219

effect on phospholipid labeling in iris muscle, 234,235 effect on the base-exchange reaction, 64,65

in brain, 241 in iris muscle, 239

effect on the incorporation of [14cJ_serine into phospholipids of the iris muscle, 239

in erythrocytes, and lipid modifications, 7 inhibition of incorporation of fatty acids in synaptosomal phospholipids by, 190

ionophore, 7 mediated exchange mechanism, 163 stimulation of incorporation of oleate into phospholipids, 216

stimulation of phosphatidylinositol phosphohydrolase, 234 transport in synaptosomes, 169,184,193 uptake

in mitochondria, effect of oligomycin on, 184 ouabain on, 184

in synaptosomes, effect of ethanol on, 185

384 SUBJECT INDEX

oligomycin on, 184-ouabain on, 184-phospholipase Con, 184 phospholipase Don, 184

Carbamylcholine and brain unesterified fatty acids, 211 and breakdown of phosphatidylinositol, 5 and incorporation of 32Pi into iris muscle phospholipids, 229 effect on phosphatidylinositol metabolism, 206 stimulation -of phospholipase A2 in plasma membranes, 212

Cardiolipin fatty acid composition in brain, 33 fatty acid composit~on in liver, 33

Catecholamine release from adrenal medulla, 278

Cationic amphiphilic drugs and anionic lipids, 6 and phosphatidic acid-phosphatase, 244

Caudate nucleus CDP-choline, in, 164 phosphatidylcholine, in, 164 phosphorylcholine, in, 164

CDP-choline and synthesis of phosphatidylcholine, 162 diacylglycerol, 5 in caudate nucleus, 164

CDP-diglyceride accumulation in iris muscle stimulated by DL-propranolol,

244 accumulation in pineal gland stimulated by DL-propranolol,237 and serine incorporation into phosphatidylserine, 237 and synaptic transmission, 199 chemical characterization, 28 fatty acid composition, 32,33 in brain, 27 in liver, 27 metabolism in the pineal gland, 149-158 molecular species in brain, 32,34,35 occurrence in the pineal gland, 150 positional distribution of fatty acids in brain, 34 synthetic, utilization for phosphatidylinositol synthesis, 32

SUBJECT INDEX 385

Cell culture studies of glycospbingolipid metabolism in brain, 131-137 studies of phospholipid metabolism in brain, 115-122,131-137

Ceramide in erythrocyte membranes, 8

Cerebellum acetylcholinesterase, in, 165 choline acetyltransferase, in, 165 choline kinase, in, 165 synaptosomes from and choline metabolism, 166 uptake of choline, in, 163

Channels ionic, 22

Charges negative, in inner membrane surface, 21 surface, in neuronal membranes, 21

Cholesterol in brain cortex phospholipid mixture, 274 in diet, 293 molar ratio to phospholipids,

in myelin, 172 in sYnaptosomal plasma membranes, 172

Cholesterol ester formation enzymatic activities, 348 in brain. in demyelinating diseases, 347 in brain with brain-blood barrier changes, 349

Choline glycerophospholipid acylation of, and cyclic-AMP, 220 incorporation in myelin, 98 incorporation into lipid by base-exchange reaction, 64 incorporation into lipid of brain cultured cells, 118-120 incorporation into phospholipid of iris muscle microsomes, 239

incorporation in vivo into phosphoglycerides of neurons and glia, 56,123

intracerebral injection, 163 metabolism

in cerebellum, 165 in cortex, 165

phosphate cytidyltranferase in cytosol, 162 phosphotransferase, in endoplasmic reticulum, 162 uptake in cerebellum, 163

386

in cortex, 163 in hypothalamus, 163 in mid-brain, 163 in striatum, 163

Choline kinase and Mg 2+ , 161 in cerebellum, 165 in cortex, 165 in synaptosomes, 164 role of, in brain, 161

Choline plasmalogens content in neurons and glia,126 formation in brain, 162 synthesis in neurinoma, 134 turnover in neurons and glia,123,124,127

Choline phospho glycerides content in brain cultured cells, 117 movement of, in myelin, 110 turnover in neurons and glia, 123,127

Cholinergic fibres stimulation from phospholipids, 290

SUBJECT INDEX

Cholinergic-link theory and 32pi labelling of phospholipids, 250, 253

Cholinergic stimulation and phosphatidylinositol,9

Clostridium perfringens phospholipase C from, 8

Conductance + + transmembrane, of Na and K , 21

Convulsive drugs and unesterified fatty acids in brain, 211

Cortex acetylcholinesterase in, 165 choline acetyltransferase in, 165 choline kinase in, 165 effect of hemicholinium on choline-uptake, 164 uptake of choline, in, 163

Cyanide sensitive factor for plasmalogen synthesis, 60

Cyclic-AMP activation of phospholipase A, 93,217 changes after brain cortex phospholipid administration, 308 changes in brain of atherogenic diet-fed animals, 301

SUBJECT INDEX 387

effect of phospholipids upon levels in brain, 93 stimulation of acyl-CoA formation, 222 stimulation of acylation of choline glycerophospholipids by, 219

stimulation of hydrolysis of phosphatidylcholine, 217,220,222 stimulation of uptake of unesterified fatty acids in synaptic membranes, by, 218

Cyclic-CMP stimulation of hydrolysis of phosphatidylcholine, 220

Cyclic-dMP 2 elevation by Ca +, 235 stimulation of hydrolysis of phosphatidylcholine, 220 stimulation of production of, by neurotransmitters, 217

Cyclic nucleotides effects on phospholipid metabolism in synaptic membranes, 211,217

stimulation of the phospholipase A2 -acylation system, 217 Cyclic-liMP

stimulation of hydrolysis of phosphatidylcholine, 220 Cytidine, incorporation into cytidine diphosphate diglyceride,

150,151 Cytidine, incorporation into phospholipids in iris muscle, 238

Cytidine nucleotides effect of, on the incorporation of serine into phospholipids, 239

Cytidine pathway in iris muscle, 244 in phosphatidylcholine synthesis, 161,163

Cytosol stimulation of fatty acid incorporation in synaptosomal pho­spholipids, by, 190

Cytidylyltransferases activation by phosphoglycerides in vivo, 89-91 activation by phosphoglycerides in vitro, 81-85

Decarboxylase converting membrane phosphatidylserine to phosphatidyletha­nolamine, 4

Denervation and phospholipid labelling in iris muscle, 245-253

388 SUBJECT INDEX

Deoxycytidine diphosphate diglyceride, synthesis in the pineal gland, 151,152

Deoxycytidine, incorporation into deoxycytidine diphosphate digly­ceride in the pineal gland, 151,152

Detergents and lipid environment, 3 effects on membranes, 175

Diacylglycerols . 2+

activation of productlon by Ca , 9 and synaptic transmission, 199 effect of myoinositol 1,2-cyclic phosphate 257-263

on production of,

formation in plasma membranes and "phospholipid effect", 227,233

increase in pancreas, stimulated by acetylcholine, 232 in erythrocyte membranes, 7,8 kinase,

activation of, by norepinephrine, 230 and "phospholipid effect", 230,232 and propranolol in iris muscle, 244 in erythrocytes, 7

metabolism in erythrocyte membranes, 7 pools in retina, 141 production, 257 production from phosphatidate in retina, 141,143 synthesis in retina, 140,141

Diacyl-GPC incorporation of labelled oleic acid into, 186,190 molecular species, labelling in brain by base-exchange, 68 pools in brain, 37 turnover in brain, 37,47 turnover in neurons and glia, 124,125,127

Diacyl-GPE molecular species, labelling in brain by base-exchange, 68 pools in brain, 37 specific radioactivity of, after intracerebral injection of labelled oleic acid, 188

turnover in brain, 37,47 turnover in neurons and glia, 125,127

Diacyl-GPI incorporation of labelled arachidonic acid into, 186,190

Dietary effects on membrane acyl groups, 169

SUBJECT INDEX 389

on synaptosomal phospholipid metabolism, 169 Diglyceride lipase

stimulation of, in synaptosomes, 211 Dinitrophenol

inhibition of incorporation of fatty acid into synaptosomal phospholipids, by, 190

Diphosphatidyl glycerol in olfactory nerve of garfish, 23

Dopamine fall in brain after brain cortex phospholipid administration, 308

stimulation of phospholipase A2 in synaptic membranes,by, 212

Dose-effect relationship in phospholipid acetylcholine output from cerebral cortex, 288,289

Drugs cationic amphiphilic, 5 cationic amphiphilic and phosphatidic acid phosphatase, 244 convulsive, and unesterified fatty acids in brain, 211

Dystrophic nerve phospholipid metabolism, 369

Echinocyte, 7

E.E.G. activation during acetylcholine output, 286

Electrical stimulation phosphatidate metabolism in synaptosomes, 199-209 phosphatidylinositol metabolism in synaptosomes, 199-209

Electroshock effect on brain unesterified fatty acids, 211 effect on brain lipids and free fatty acids, 322

Endocytosis and membrane fusion, 9

Endoplasmic reticulum synthesis of phospholipids in, 95

Enzyme activation by lipid, 175 lipid depletion of, 175

Erythrocytes ageing~ 7 and Ca +, 7 and diacylglycerol, 7,8

390

ghosts, 7 membrane in diseases and lipid metabolism, 7 membrane shape and lipid composition, 7 phosphatidylinositol in, 4

SUBJECT INDEX

phospholipids in normal and dystrophic mice, 370 Eserine

intraventricular injection of, and unesterified fatty acids, 211

ESR spectrum in mitochondria, 17

Essential fatty acid deficiency, 169,187 reversal of, 169,187 effect on incorporation of fatty acids into synaptosomal phospholipids, 190

Ethanol (see also alcoho.1) effect

on acyltransferases, 191 on ATPase, 180 on incorporation of fatty acids in synaptosomal phospho-lipids, 190,191

on ionic conductance, 180 on norepinephrine uptake, 184 on phosphoenzyme intermediate of ATPase, 181 on synaptosomal phospholipids, 169

Ethanolamine and base exchange, 38 effect of intracerebral injection in brain, 37,38 incorporation in vivo into phosphoglycerides of neurons and glia, 56,123

incorporation into brain lipid by base-exchange, 64 incorporation into ethanolamine phospho glycerides of brain subcellular fraction, 43

incorporation into lipid of brain cultured cells, 118-119 incorporation into myelin ethanolamine phosphoglycerides,43 incorporation into phosphoglycerides, 42 kinase in brain, 166 phosphorylation of, in brain, 166

Ethanolamine plasmalogens content in brain cultured cells, 117 content in neurons and glia, 126 requirement of microsomal electron transport system for its synthesis, 58,59

SUBJECT INDEX 391

synthesis by base-exchange, 67 synthesis from alkylacyl-GPE,38,56 synthesis from alkylacyl-GPE in neurons and glia, 56 synthesis in neurinoma, 134 turnover in brain, 38,55 turnover in glia, 55,125,127 turnover in neurons, 55,125,127

Ethanolamine phospho glycerides content in brain cultured cells, 117 requirement of cytidine nucleotides for their synthesis, 38 requirement of cytidine nucleotides in neurons and glia, 56 synthesis in brain, 38 turnover in neurons and glia, 123,127

Exchange 2 mechanism, Ca + mediated, 163 of lipids in myelin, 101

Exocytosis and membrane fusion, 9

Fatty acids (see also acyl groups) changes in brain of dietary treated animals, 294 conversion to lipid in retina, 142 conversion to phospholipid in brain cultured cells, 116 conversion to triglycerides in retina, 142 deficiency and ATPase, 179 effect by phospholipase A2 treatment, in synaptosomes, 175 free, in brain, 339 free, in phospholipase-treated synaptic membranes, 177 in brain cortex phospholipid mixture, 274 in essential fatty acid deficiency, 338,342 incorporation into synaptosomal phospholipids,

effect of ouabain on, 190 effect of temperature on, 190 effects of essential fatty acid deficiency, 190 inhibition by Ca2+, 190 inhibition by dinitrophenol (DNP), 190 inhibition by para-chloro-mercuri-benzoic acid (PCMB),

190 stimulation by cytosol, 190 stimulation by glucose, 190

labelled, incorporation in phosphoglycerides, 186 metabolism in brain cultured cells, 116 of lysophosphoglycerides in synaptosomes, 175

392

FCCP

polynsaturated, formation of lipoperoxide from, 178 in brain, 337 in brain of dietary induced animals, 337,338 in myelin glycerophospholipids, 172 in synaptosomal plasma membranes, 172

spin label, in mitochondria, 17

SUBJECT INDEX

turnover in phosphoglycerides of brain, 47,48 turnover of (n-9) in membrane phosphoglycerides, 189

in synaptosomal alkenyl acyl-GPE, 189 in synaptosomal diacyl-GPE, 189

(s) unesterified, in brain, 211 effects of convulsive drugs, on, 211 effects of electroshock, on, 211 effects of intraventricularly injected carbamoylcholine,

211

effects of intraventricularly injected eserine, on, 211 in incubated synaptosomes, and iproniazide phosphate,

211

in synaptosomes, effect of norepinephrine on, 211 release and uptake of, by synaptic membranes, 211

uncoupling action of, 16 Fluoride (NaF)

effects on fatty acid incorporation into phospholipids, 219 Free Fatty acids

Gab a

content in brain, 318 content in brain during ischemia, 319,342 content in retina, 325 content in retina during ischemia, 325 in cerebrospinal fluid and neurological diseases, 332

effect of phospholipase A and C on the binding, 267,268 effect of phospholipids on gaba receptor, 265-268 effect of phospholipids on the binding,267,268 stimulation of cyclic-AMP formation, 217 stimulation of phospholipase A2 in sub synaptosomal membra­nes, by, 212

Ganglia, superior cervical 1 . 1 . 1· f d 32p . . t . . t h e ectrlca stlmu atlon 0 , an 1 lncorpora lon ln 0 p 0-

sphatidylinositol, 252

SUBJECT INDEX

Ganglionectomy, superior cervical and 32pi incorporation into iris muscle phospholipids,245 and 32pi incorporation into iris muscle phospholipids in vivo, 249

Gangliosides, 6

Glia and negative charges in axons, 23

choline plasmalogen turnover, 123,124,127 diacyl-GPC turnover, 124,125,127 diacyl-GPE turnover, 125,127 ethanolamine plasmalogen turnover, 125,127 formation of lipids in, 112 plasmalogen synthesis, 55,123 phosphoglyceride synthesis, 55,123 separation from cell types, 131 synthesis of phosphatidylcholine in, 162

Glicerophosphinicocholine action of enzymes on, 162

Glucose and "phospholipid effect" in iris muscle, 228 content in brain tissue, 271 effect of drugs on free cerebral glucose, 272 effect of polar lipids on blood glucose, 274

393

effect of polar lipids on free cerebral glucose content,271 stimulation of incorporation of fatty acids into synaptosomal phospholipids, 190

Glutamic acid stimulation of cyclic-GMP formation, by, 217 stimulation of phospholipase A2 in sub synaptosomal membra­nes, by, 212

Glycerol effect of intracerebral injection in brain, 37 effect of propranolol and phentolamine on incorporation in-to retina lipid, 143-145

incorporation into brain diacyl-GPE and diacyl-GPC, 46 incorporation into retina glycerolipids,139-148 oxidation in retina, 141 turnover rate in brain lipids, 44,45

Glycerolipids in membranes, 3,4,7 synthesis in the retina, 139-148

Glycolipid biosynthesis,6 in myelin, 172

394

Glycolipids in brain cortex phospholipid mixture, 274

Glycoprotein biosynthesis and polyprenol phosphate derivatives,6

Glycosphingolipid synthesis in neurinoma, 131-137, 134 turnover in neurinoma, 131-137

Gouy-Chapman theory and membrane potential, 21

Hematoside synthesis in neurinoma, 136

Hemicholinium-3 inhibition of choline kinase, 164

SUBJECT INDEX

stimulation of choline uptake in phospholipids, 164 Hereditary spherocytosis

and erythrocyte membranes, 7 Histamine

stimulation of phospholipase A2 in synaptic membranes, by, 212

Homogenization effect of lipids on glucose distribution in mice, 275

Homovanillic acid change in brain after brain cortex phospholipids administra tion, 308

Hydergine abolishing hyperglicemia, 276

5-Hydroxytryptamine effect on unesterified fatty acids in synaptosomes, 211 stimulation of lipases in synaptosomes, by, 211 stimulation of phospholipase A2 in synaptic membranes, by, 212

Hyperglycemia lipid-induced, 276

Hypophysectomized mices effect of lipid-induced brain glucose changes, 278

Hypothalamus uptake of choline, 163

Indomethacin on free arachidonate production in brain, 322

SUBJECT INDEX

Inositol metabolism in the pineal gland, 152-157

Inositol phosphoglycerides incorporation of arachidonic acid into, 186

Insulin effect on brain glucose, 272,273

Ionophore treatment of cells with,9

Iproniazide phosphate

395

effect of, on unesterified fatty acids in incubated synapto­somes, 211

Iris muscle, rabbit cholinergic and adrenergic innervation, in, 227 denervation of, and "phospholipid effect", in, 245 phospholipid metabolism, in, 227

Isoproterenol and the "phospholipid effect" in iris muscle, 230

Junctional complexes effect of phospholipases A and C on Gaba binding, 267,268 Gaba binding, 267

+ K conductance of squid axon, 21

K+ efflux from parotid and Ca2+, 235

Lactate in lipid-induced hyperglycemia, 278

Lactosyl ceramide synthesis in neurinoma, 136

Lateral movement of phosphatidylcholine in myelin, 111-112

Layers inner and outer, in myelin, 111

Lecithin (see phosphatidylcholine)

Lecithin-cholesterol acyl transferase activity (LCAT) in brain damage, 349 in brain homogenate,351 in normal brain, 349

396

in spinal fluid, 349 in plasma, 348

Lidocaine effect on brain glucose, 273

Linoleic acid in diet, 338

Linolenic acid in diet, 338

Lipid addition, 3,6

Lipid environment, 6 and detergents,3

Lipid metabolism in neurinoma, 131-137 stimulation of enzymes by phospholipids, 79

Lipid removal, 3,6

Liposomes penetration in eNS, 282

Lipoxidation effect on synaptosomes, 169 of polyunsaturated fatty acids

and ATPase inactivation, 178 in synaptic membranes, 178

Lymphocyte phosphatidylinositol in, 6

"Lyso-bis-phosphatidic acid" metabolism, 359 occurrence in mammal tissues, 357,358

Lysolecithin

SUBJECT INDEX

and incorporation of oleate into phospholipids, 177,216 Lysophosphatidylethanolamine

in brain cortex phospholipid mixture, 274 Lysophosphoglycerides

acyl groups of, in synaptosomes, 175 formation in synaptosomes, 177

Lysophospholipase in brain, 42,212 in synaptosomes, 212

Lysosomes and phospholipid, 360,361 in cell storage phenomena, 360,361

SUBJECT INDEX

Malarian infestation and erythrocyte membranes, 7

Membranes acyl groups, modification by diet, 169 effect of

alcohol on, 175 detergents on, 175 diet on acyl groups in, 179

397

enzymatic activities in, and phosphatidilinositol and serine, 4

external base-exchange in, 164 fusion, 10 glycerolipids in, 3 in dietary induced changes of phospholipids, 338 inner and external leaflet, 8 internal membrane lipids, 4 Na+/0" -ATPase in, 4 neuronal, and surface charges, 21 permeability, and phospholipid hydrolysis, 17

2+ polarization of, and ATPase, 174 Mg

and choline kinase activity, 161

Microsomes 14 14 from iris muscle, incorporation of[ C] serine and[ 9 choli­ne into phospholipids, 239

incorporation of fatty acids in phosphoglycerides, 189 transfer of phospholipids from, to myelin, 95

Microwave irradiation in sacrifyinganimals, 340

Mid-brain uptake of choline in, 163

Mitochondria effect of phospholipase Con, 15 fluorescence, in tightly coupled state, 15 NADH in, 15 particles, 15 sonication and lipid-protein interactions, 19 swelling of, and permeability, 16 uncoupling of, 16

Monoacylglyceryl (bis)-phosphate accumulation in lysosomes, 350,361 and lysosomes, 362

398

in liver of triton-treated rats, 361 in thesaurismosis, 360 involvement in storage phenomena, 360 structure, 358,359

Monoacylphosphoglycerides in brain ischemia, 320

Monoamine oxidase inhibitors

SUBJECT INDEX

and unesterified fatty acids in incubated synaptosomes, 211 Monohexosyl ceramide synthesis in neurinoma, 134-136

Muscular dystrophy murine, phospholipid metabolism, 367

Myelin alkenylacyl-glycerophosphorylethanolamine, in, 172 CNS, from optic nerve, morphology of, 97 exchange of lipids in, 101 glycolipids in, 172 incorporation of label in, 107 lipids, 170 molar ratio of cholesterol to phospholipids, 172 phospholipid composition of, 173 polyunsaturated fatty acids in, 172-173 PNS, from trigeminal nerve, morphology of, 97 protein profiles, 174 sheath, renewal of phospholipid in,95 turnover of constituents, 98,112

Myoinositol

NADH

cyclic, metabolism in smooth muscle,257-263 cyclic, production,257 metabolism in the pineal gland, 152-157

+ +fluorescence of, 15 Na /K ATPase

and ATP-ADP exchange, 174 inhibition by ethanol, 180 inhibition by phospholipase C and D, 177 in plasma membranes, 4 in synaptosomal plasma membranes, 169,174 membrane-bound phosphoenzyme intermediate, 181

Nerve-conduction in squid giant axon, 21

SUBJECT INDEX

Nerve-endings and choline uptake, 163 phoshoryl choline in, 164

Neurinoma glycolipid content, 133 lipid labelling, 133 plasmalogen synthesis, 134 phospholipid content, 133

Neuroblastoma glycolipid metabolism, 131-137 glycosphingolipid metabolism, 131-137 phospholipid metabolism, 131-137

Neurons choline plasmalogen turnover, 123,124 diacyl-GPC turnover, 124,125,127 diacyl-GPE turnover, 125,127 ethanolamine plasmalogen turnover, 125,127 plasmalogen synthesis, 55,123 phosphoglyceride synthesis, 55,123 separation from other cell types, 131 synthesis of phosphatidylcholine in, 162

Neuropharmacological agents effect on phospholipid metabolism in iris muscle, 227

Neurotransmitters effect on

phosphatidylinositol turnover, 258 phospholipid metabolism in the pineal gland, 149 phospholipid metabolism in various tissues, 257

effects on acylation-de acylation in brain synaptosomes, 194 effects on the metabolism of phospholipids in synaptic mem­branes, 211

399

effects on phospholipid metabolism in rabbit iris muscle, 227 production in cultured brain cells, 115

Noradrenaline (see norepinephrine)

Norepinephrine and increased incorporation of 32pi into phosphatidic acid in denervated iris muscle, 247,250

and unesterified fatty acids in synaptosomes, 211 and "phospholipid effect" in iris muscle after ganglionecto­my, 245

and stimulation of acyl-CoA formation, 222 and stimulation of hydrolysis of phosphatidylcholine in sy-

400 SUBJECT INDEX

naptic membranes, 215 and stimulation of hydrolysis of phosphatidylinositol and phosphatidic acid in iris muscle, 233

and stimulation of uptake of oleate by synaptic membranes, 215

changes in brain after cerebral phospholipid administration,

308 32 effect on Pi incorporation in iris muscle phospholipids, 227

stimulation of incorporation of oleate into glyceropqospho-lipids, 215

stimulation of lipases in synaptosomes, 211,212,213 supersensitivity to, in denervated eyes, 245 uptake of

effect of phospholipases. on, 183,194 in synaptosomes, 183

Oleic acid incorporation into glycerophospholipids, 221

stimulation by CaC12' 220 stimulation by cyclic nucleotides, 220 stimulation by norepinephrine, 215

labelled, incorporation into diacyl-GPC, 186 Olfactory nerve

of garfish, 23 Oligodendroglia

synthesis of ethanolamine plasmalogens, 56,57 turnover of phosphoglycerides, 50

Palmitate effect of propranolol and phentolamine on incorporation into retina lipid, 143,145

incorporation into brain glyceride, 212 incorporation into retina glycerolipid, 139-148

[1- 14C] , intracerebral injection, 186 Para-chloro-mercuri-benzoic acid (PCMB)

inhibition of incorporation of fatty acids into synaptoso­mal phospholipids by, 190

Penthylentetrazol effect on brain glucose, 275

SUBJECT INDEX

pH

effect on base-exchange reactions, 65 Phenol red

and uncoupling of mitochondria, 16 Phenothiazine derivatives, 5

and phosphatidylinositol labelling, 5 Phentolamine

effect on phosphatidate phosphohydrolase, 148 phospholipid synthesis in pineal gland, 151 phospholipid synthesis in retina, 143-145

Phenylephrine and "phospholipid effect" in iris muscle, 230

Phosphatidate (see also phosphatidic acid) increase, stimulated by acetylcholine, in pancreas, 232 membrane-bound, 6 metabolism in erythrocytes, 7

Phosphatidic acid and synaptic transmission, 199,200 atypical pool in brain, 36 breakdown in synaptosomes, and acetylcholine, 232 fatty acid composition in brain, 32,33 hydrolysis, effect of propranolol and phentolamine, 146 hydrolysis in synaptosomes, 146 in brain, 29,30 in brain cortex phospholipid mixture, 274 incorporation of

oleate into, 216 32pi into, 227

401

32pi into, in denervated iris muscle, and norepinephri­ne, 247

32pi into, stimulated by acetylcholine, 229 increase, in iris muscle, by norepinephrine, 232 in liver, 29,30 in olfactory nerve of garfish, 23 labelling in denervated iris muscle, 242,247

and supersensitivity, 251 molecular species of, 32 synthesis in retina, 140,141

Phosphatidic acid phosphatase and cationic amphiphilic drugs, 244 and "phospholipid effect", 230

402

and DL-propranolol, 244 inhibition by drugs, 5,6 regulation by, 146

SUBJECT INDEX

Phosphatidylcholine action of phospholipase Con, 162

2+ acylation of, cyclic-AMP-stimulated, and Ca , 220 effect on brain cortex acetylcholine output, 288 hydrolysis of, cyclic AMP-stimulated, and ATP, 220 hydrolysis of, stimulated by cyclic nucleotides, 220 in brain cortex phospholipid mixture, 274 in erythrocytes, 8 in olfactory nerve of garfish, 23 incorporation of choline into, 161 incorporation of[3I!l choline in, of PNS myelin, 102 inhibition of labelling by propranolol, 237

Phosphatidylethanolamine effect on brain cortex acetylcholine output, 288 in brain cortex phospholipid mixture,·274 in olfactory nerve of garfish, 23

Phosphatidylglycerol in mammalian tissue, 357,358 in pineal gland, metabolism, 152-157

Phosphatidylinositol, 5 and membrane enzymatic acti vi ties, 4 and synaptic transmission, 199,265 breakdown, 232,257,258 decrease, in iris muscle, and norepinephrine, 232 fatty acid composition in brain, 33 fatty acid composition in liver, 33 in brain, 27,29,30 incorporation of oleate into, 216 incorporation of 32pi into, 227,229 in erythrocytes, 4,8 in olfactory nerve of garfish, 23 inositol phosphohydrolase and "phospholipid effect", 230, 234

labelling and drugs, 5 metabolism in smooth muscle, 257-263 metabolism in the pineal gland, 152-157 molecular species in brain, 27 specific phospholipase C, 4 synthesis from cytidine diphosphate diglyceride, 27,31 turnover and receptors, 5,232

SUBJECT INDEX

turnover in ganglia and d-tubocurarine, 245 Phosphatidylserine

and membrane enzymatic activities, 4 effect on brain cortex acetylcholine output, 288 effect on brain free glucose, 280 in brain cortex phospholipid mixture, 274 in olfactory nerve of garfish, 23 purification, 278

Phosphoglycerides (see also phospholipids) alternate pathways of synthesis in retina, 147 and lysosomal damage, 360,361 catabolism in brain, 37 exchange between membranes, 50 exchange between organelles, 51 in liver of triton-treated rats, 361 metabolism in

brain, regulation, 79-94 cell storage phenomena, 357-365 cultured brain cells, 115-122

pools in brain, 48,49 pools, labelling by base-exchange, 70-72 rate-limiting reaction in biosynthesis, 85,86 regulation of synthesis in retina, 146 requirement for base-exchange reactions, 67 role in neurotransmission, 199-209,266 synthesis by base-exchange, 63,67 synthesis in brain, 37

rate of enzymic reactions, 80 synthesis in neurinoma, 131-137 transfer from blood to brain, 86-88 turnover

in brain, 37 in glia, 55 in neurons, 55 in specific cell types of brain, 49,50,55 of acyl groups in membranes, 169

Phospholipase A in brain damage by ischemia, 331 regulation in excitable membranes, 328

Phospholipase Al in brain, 211 in synaptosomes, 212

403

404

Phospholipase A2 and hydrolysis of phosphatidylcholine, 222 and synaptosomal acylations, 193 effect on fatty acids insynaptosomes, 176 from snake (Crotalus Adamanteus) venom, 223 in brain, 211 in synaptosomes, 212 net activity, in synaptosomes, 212

SUBJECT INDEX

stimulation in synaptic membranes by neurotransmitters, 212

treatment of synaptosomal membranes by, 175 Phospholipase A1 and A2

effect on cholinergic receptors, 265 Gaba binding, 267,268 neurotransmission, 265,268

in brain, 40,211 in glia, 40,55 in neurons, 40,55

Phospholipase C action on phosphatidylcholine, 162 activity on phospholipids, 147,257 effect on

cholinergic receptors, 265 Gaba binding, 267,268 mitochondria, 15

of Clostridium perfrigens, 8 phosphatidylethanolamine-specific, 11 phosphatidy linosi tol-specific, 4 stimulated by Ca2+, 8 treatment of synaptosomal membranes, 173

, and inhibition of ATPase, 175 Phospholipase D

and membrane fusion, 11 in rat· brain, 64 treatment of synaptosomal membranes, 175

, and inhibition of ATPase, 177 Phospholipases, 3

effect on norepinephrine uptake, 183 treatment of cells, 9

of synaptosomes, 169,175 Phospholipid biosynthesis

major pathways, 244

SUBJECT INDEX

"Phospholipid effect" in adrenal medulla, 235 in ganglia, and d-tubocurarine, 245 in iris muscle, 227

and adrenergic agonists and antagonists, 231 and adrenergic blockers, 230 and diglyceride kinase, 230 and isoproterenol, 230 and phenylephrine, 230 and phosphatidic acid-phosphatase, 230 and phosphatidylinositol-phosphohydrolase, 230 and preincubation with 32pi, 228 after superior cervical ganglionectomy, 245 effect of

ageing on, 234 Ca2+ on, 234,235 glucose on, 228 temperature on, 228

post junctional location of, 248 in pancreas, 235 in thyroid, 235

Phospholipid labelling in iris muscle and cholinergic-link theory, 250

Phospholipid metabolism and DL-propranolol, in iris muscle, 235 hydrolysis and acridine orange response, 16 in murine muscolar dystrophy, 369, in rabbit iris muscle,

effect of neurotransmitters on, 227 effect of propranolol on, 228

in synaptic membranes, effect of neurotransmitters on, 211 in vivo, in synaptosomes, 189

Phospholipids and brain acetylcholine, 285 exchange in subcellular fractions, 95 exchange proteins, 5 from soya beans, 287 half-lives in normal and dystrophic mice, 370 hydrolysis, and mitochondrial membranes, 16 in myelin, 95 lateral mobility, 22 transfer from microsomes to myelin, 95

405

SUBJECT INDEX

Phosphomonoesterase, 6

Phosphorylcholine and low affinity uptake of choline, 166 conversion to lipid, 73-76,120 cytidylyltransferase, activation by lysophospholipids, 83-85

cytidylyltransferase, activation by phospholipids, 81-85 in brain tissue, 161,162 in caudate nucleus, 164 in cortex, 164 in nerve endings, 164 in vivo, metabolism in brain, 73-76 stimulation of hydrolysis by norepinephrine, 214 synthesis in brain cultured cells, 119-121

Phosphorylethanolamine conversion to lipid, 73-76,120 cytidylyltransferase, 86 in vivo, metabolism in brain, 73-76 synthesis in brain cultured cells, 119-121

Physical status of lipid dispersion, 274

Pineal gland effect of adrenoreceptors on phospholipid synthesis, 149 effect of neurotransmitters on phospholipid synthesis, 149 incorporation of 32pi into phospholipids, in, 237 metabolism of

cytidine-diphosphate diglyceride, 149-158 phosphatidylglycerol, 152-157

32 phosphatidylinositol, 152-157 P -phosphate

incorporation and a adrenergic receptors, 230 in atherogenic diet-fed animals, 298,300 in brain phospholipids, stimulated by acetylcholine, 227

in iris muscle phospholipids after superior cervical ganglionectomy, 245,247 after superior cervical ganglionectomy in vivo, 249 and cholinergic-link theory, 250 effect of

acetylcholine on, 249 adrenergic agonists and antagonists on, 230

406

SUBJECT INDEX

neurotransmitters and pharmacological agents on, 227

norepinephrine on, 233 propranolol on, 241,243

in myelin, 98 in nervous tissue phospholipids of dystrophic mice, 368

in phosphatidic acid and phosphatidylinositol in iris muscle

effect of acetylcholine after denervation on, 248

effect of acetylcholine on, 229,248 into phosphatidylinositol in stimulated ganglia, 252

into phosphatidic acid in denervated iris muscle, and norepinephrine, 247

stimulation by ~-blockers, 230 Plasma membrane +

efflux of N; , 175 influx of K , 175 Na+-I0- ATPase in, 4 of synaptosomes, 170

Plasmalogenase in brain, 40 properties, 40

Plasmalogen synthesis in neurinoma, 134

Polar lipids, 3 and free cerebral glucose content, 271

Polyprenol phosphate derivatives and glycoprotein biosynthesis, 6

Potential Galvani, in membranes, 22 zeta, in membranes, 22

Propranolol accumulation of CDP-diglyceride in lrlS muscle, by, 244 accumulation of CDP-diglyceride, in rat pineal, by, 237 and diglyceride-kinase, 244 and phosphatidic acid-phosphatase, 244 and phospholipid metabolism in iris muscle, 235 effect on

base-exchange reactions in iris muscle, 237,239

407

408 SUBJECT INDEX

C14e]-choline incorporation in iris muscle phospholipids,

[ !fOe] .. .. .. 1 h h 1· ·d serlne lncorporatlon ln lrlS musc e p osp 0 lpl s, 239 incorporation of 32pi into phospholipids, 242 phosphatidate phosphohydrolase, 146 phospholipid synthesis in pineal gland, 149,150 phospholipid synthesis in retina, 143-145

inhibition of labelling of phosphatidylcholine in iris mu­scle, 237,242

stimulation of labelling of phosphatidic acid and phosphat! dylinositol in iris muscle, 242

stimulation of labelling of phospholipids in iris muscle, 237

Protein phosphate phosphatase, inhibition of, by ions, 215

Pyridoxal-kinase, activation by phospholipids, 92

Ranvier nodes of, 22

Receptors

Retina

a adrenergic, 5 a adrenergic, and lIphospholipid effect ll in iris muscle, 230

in other tissues, 230 muscarinic cholinergic,S

effect of propranolol and phentolamine on glycerolipid synthesis, 143-145 for studies on membrane lipids, 325 glycerolipid synthesis

in cattle, 139-148 in the toad, 139-148

Safranine response and coupling of mitochondria, 16

Schwann cells uptake of label in autoradiography, 105-108

Sciatic nerve autoradiography of, 104 lipids in, 103 phospholipid specific activity in dystrophic mice, 369

SUBJECT INDEX 409

Scopolamine effect on acetylcholine release from cerebral cortex,288-289

"Semi-lyso-bis-phosphatidic acid"

Serine occurrence in mammal tissue, 357,358

incorporation into brain lipid by base-exchange, 64 lipid of brain cultured cells, 120-121 phospholipid of the iris muscle, 239

plasmalogen synthesis in neurinoma, 134 Smooth muscle

and cyclic -1,2-my.oinositol phosphate, 257-263 effect of neurotransmitters on phospholipid metabolism, 257-263

Sonication effect of lipids on glucose distribution in mice, 275 of mitochondria, and lipid-protein interactions, 19

Sphingomyelin action of sphingomyelinase on, 162 in brain cortex phospholipid mixture, 274 in olfactory nerve of garfish, 23 in PNS myelin, 102

Sphingomyelinase action on sphingomyelin, 162

Spinal cord phospholipid specific activity in dystrophic mice, 369

Squid axon conductance of ions, 21

Staphylococcus aureu~ 4 phosphatidylinositol-specific-phospholipase C, 4

Stimulation, electrical f 0 0 1 1 0 d 32p o 0 0 0 o superl0r cervlca gang la an 1 lncorporatlon lnto phosphatidylinositol, 252

Stomacyte, 8

Storage phenomena monoacylglyceryl (bis)-phosphate accumulation, 360 phospholipid accumulation, 360

Striatum choline acetyltransferase in, 163 uptake of choline in, 163

Supersensitivity in denervated iris muscle and phosphatidic acid labelling, 251

410 SUBJECT INDEX

Synaptic transmission 32pi incorporation into phosphatidylinositol, 199 phosphatidate synthesis, 200

Synaptosomes acetylcholinesterase in, 170 alkenylacyl glycerophosphorylethanolamine,172-173 anionic phospholipids in, 23 ATPase in, 169,174 breakdown of phosphatidic acid, stimulated by acetylcholi-ne, in, 232

calcium transport in, 169 cerebellar, 166 choline kinase in, 164 effect of

ethanol on, 169 iproniazide phosphate on unesterified fatty acids, in, 211

lipoxidation, 169 norepinephrine on hydrolysis of phosphatidylcholine, in, 214,215

norepinephrine on phospholipase A2, in synaptosomal fractions, 212

norepinephrine on unesterified fatty acids, in, 211 norepinephrine on uptake of oleate, in, 214,215 neurotransmitters on acylation-de acylation cycle, in, 194

hypo-osmotic shock and choline kinase activity, 166 incorporation of fatty acids into phospholipids, in, 190

effect of essential fatty acid deficiency on, 190 effect of ethanol on, 190,191

lipoxidation of polyunsaturated fatty acids of, 178 membranes and phospholipids, 169 membranes of, effects of neurotransmitters on phospholipid metabolism, in, 211

effects of cyclic nucleotides, 211 metabolism of phospholipids "in vitro" in, 189 molar ratio of cholesterol to phospholipids in, 172 on phospholipid metabolism, in, 211 phospholipase treatment of, 169,174 phospholipid composition of, 173 phospholipid metabolism during electrical stimulation, 199-209

phospholipid metabolism in, 169

SUBJECT INDEX

dietary effect on, 169 plasma membranes of, 170 polyunsaturated fatty acids in, 172-173 preparation of, 170-171 protein profiles of, 172-173

in guinea pig, 172 in human, 172 in mouse, 172 in rhesus monkey, 172 in squirrel monkey, 172

uptake of choline in, 163 of norepinephrine in, 169,183,194

Temperature effect on incorporation of fatty acids into synaptosomal phospholipids, 190

Theophylline and phospholipase A2-acylation system in synaptic membra­nes, 217

Thesaurismosis monoacylglyceryl (bis)-phosphate accumulation,36 phospholipid accumulation, 36

Transferases effects of serum transferases on brain phospholipid meta­bolism,347

Trienoic fatty acids accumulation in brain, during EFA deficiencY,338

Triglycerides

411

cerebral concentration and production of unesterified fatty acids, 211

conversion of fatty acids to phospholipids,116-118 formation from fatty acids in retina, 142 in brain during ischemia, 320 lipase, in synaptosomes, stimulation of, 211 metabolism in cultured brain cells, 116-118 synthesis in retina, 140,141

Triphosphoinositide hydrolysis of, 6

D-Tubocurarine, and "phospholipid effect" in ganglia, 245

412

Uptake of Gaba, 267,268 high affinity, of choline, 163

, Na+ dependent, 163 low affinity, of choline, 163

inhibition by hemicolinium, 164 of noradrenaline, 262 of choline, 161-163 of norepinephrine

by synaptosomes, 183

SUBJECT INDEX