Museum_Meeting_April_2013
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John James Wilson Museum of Zoology, Institute of Biological Sciences, Faculty of Science, University of Malaya [email protected]
description
My talk on Museum of Zoology, University of Malaya, Outputs on April 5th 2013
Transcript of Museum_Meeting_April_2013
John James WilsonMuseum of Zoology, Institute of Biological Sciences,Faculty of Science, University of Malaya
museumzoology.um.edu.my
Bulletin of the Museum of
Zoology
Next issue end of April 2013
Biodiversity seminars
Special exhibition
Workshop
Hunt for Vampire Moth
Biodiversity Genomics Lab
With capacity for routine DNA barcoding of all animal groups
DNA barcoding of bats
Funded by UMRG158/12SUS: Butterflies of Peninsula Malaysia Project
Testing the utility of DNA barcoding for rapid and accurate assessment of
bat diversity in Malaysia in the absence of formally described species
John James Wilson*, Sing Kong Wah*, Muhammad Rasul Abdullah Halim, Rosli bin Ramli, Rosli bin Hashim, & Mohd Sofian-Azirun
Genetics and Molecular Research, in press
How to Rapidly Accelerate Biodiversity Inventory in Regions where Most of the Species are Unknown?
Malaysia’s First DNA Barcoding BioblitzJohn James Wilson, Kong Wah Sing,
Khairunnisa Syaripuddin…..in preparation
Funded by UMRG158/12SUS: Butterflies of Peninsula Malaysia Project
Time taken for completion of DNA barcoding Bioblitz
Step Time taken (hrs)Specimen collection 12Specimen sorting 2DNA extraction 0.5PCR set-up 2PCR 3PCR check 1.52nd PCR set-up 2PCR 3PCR check 1.5DNA sequencing 48Sequence editing, alignment, upload 1Analysis of DNA barcodes 1TOTAL 77.5 (3 days)
Cost of DNA barcoding Bioblitz
Step Details of consumables/reagents
Cost of consumables/reagents/service per specimen in MYR
Specimen collection 1.5ml microcentrifuge tubes 0.07Specimen sorting 96-well plate 0.14DNA extraction Xytogen kits (100 preps),
Pipette tips2.33
PCR PCR Master mix, Primers, ddH20, 0.2ml PCR tube, Pipette
tips
0.95 (0.95)
PCR check Agarose, TAE buffer, DNA ladder (0.5μl), GelRed DNA stain
1.00 (1.00)*
DNA sequencing 21.00TOTAL 24.49 (26.49)
Building a DNA barcode reference library for the true butterflies
(Lepidoptera) of peninsula Malaysia: What about the subspecies?
John-James Wilson1,2, Kong-Wah Sing1, Mohd Sofian-Azirun2
PLoS ONE, in review
Funded by UMRG158/12SUS: Butterflies of Peninsula Malaysia Project
Fieldwork
Fieldwork assistance: Minmay Wong, Mary Wilson, Thary Goh, Sasekumar, Evelyn Leung, Nisa, LindaFunding support: LADA Field study Grant, Asian Biodiversity Centre & LESTARI
Mining collections
Global Malaise Program
Field assistance: Ahmed A/L Uda, Sasekumar, Gary Sing, Minmay Wong, Thary Goh, Aaron Teo
Thankyou
museumzoology.um.edu.my
