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Case ReportMorphological and Molecular Characterization of HumanGingival Tissue Overlying Multiple Oral Exostoses
Luca Francetti ,1,2 Claudia Dellavia ,1 Stefano Corbella ,1,2 Nicolò Cavalli ,1,2
Claudia Moscheni ,3 Elena Canciani ,1 and Nicoletta Gagliano 4
1Department of Biomedical, Surgical and Dental Sciences, Università degli Studi di Milano, via L. Mangiagalli 31, 20133 Milan, Italy2IRCCS Istituto Ortopedico Galeazzi, Via R. Galeazzi, 4, 20161 Milan, Italy3Department of Biomedical and Clinical Sciences “L. Sacco”, Università degli Studi di Milano, via G.B. Grassi 74, 20157 Milan, Italy4Department of Biomedical Sciences for Health, Università degli Studi di Milano, via L. Mangiagalli 31, 20133 Milan, Italy
Correspondence should be addressed to Nicoletta Gagliano; [email protected]
Received 6 September 2018; Accepted 10 May 2019; Published 22 May 2019
Academic Editor: Jamil Awad Shibli
Copyright © 2019 Luca Francetti et al. This is an open access article distributed under the Creative Commons Attribution License,which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Gingival and osseous augmentations are reported as hypertrophic or hyperplastic reactions to different factors including chronictraumatisms and surgeries such as free gingival graft (FGG) that induce an abnormal growth of both hard and soft tissues ingenetically predisposed subjects. Since an imbalance in collagen turnover plays a key role in the development of gingivalovergrowth leading to an accumulation of collagen in gingival connective tissue, in this study we described the histological andmolecular features of three oral overgrowths obtained from a 34-year-old woman previously operated for FGG in order toevaluate a possible relationship between exostoses and overgrown tissue. Healthy and overgrown gingiva were analyzed byhistological methods, and the expression of genes and proteins involved in collagen synthesis, maturation, and degradation wasassessed in cultured fibroblasts obtained from gingival fragments at the molecular level. Our results show that generalmorphology and collagen content were similar in healthy and overgrown gingivae. However, fibroblasts obtained from theovergrown gingiva revealed an anabolic phenotype characterized by an increased collagen turnover and maturation. Thesefindings indicate that an exostosis could act as a mechanical stimulus stretching the overlying connective tissue and triggeringan anabolic phenotype of gingival fibroblasts and suggest to use minimally invasive surgical techniques to avoid traumatizingthe periosteal tissues for the eradication of the exostosis with minimal relapses.
1. Introduction
Exostoses are described in literature as benign localized over-growths of the bone of unknown etiology [1]. They mayappear as nodular, flat, or pedunculate protuberances andmay be found in different anatomical areas; the most fre-quent are palatal and mandibular tori, but vestibular exosto-ses can also be present. Vestibular exostoses are mostcommon in the maxilla and are less frequently found onthe mandibular surface. Multiple exostoses are present lessregularly [1, 2]. Exostoses may appear as a rare side effectof free gingival graft (FGG) resulting from periosteal trauma-tism during site preparation that induces the activation ofosteoprogenitor cells and of fibroblasts contained in the
connective tissue of the graft, leading to bone and soft tissueovergrowth [1, 3].
Several studies have tried to explain the etiology, and dif-ferent hypotheses have been proposed. Chambrone andChambrone evaluated nine exostoses where FGG surgerywas performed to augment the amount of keratinized gin-giva. The authors have observed that in each of the examinedcases, intentional or accidental periosteal fenestration duringthe surgical approach has occurred [1]. Considering thehypotheses of periosteal trauma acting as the main etiologicagent, frenectomies can also be involved in exostosis forma-tion. In fact, the linear micro-fenestrations that occur duringthe preparation of a receiving site can evoke bone stimulationleading to the development of exostoses [2]. In three cases
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described in literature, the exostoses appeared in graftedareas associated with teeth that had supported removable orfixed prostheses. It is possible that the combination of perios-teal fenestration and occlusal function as a continuous irrita-tion factor is responsible for this osseous proliferation [4–6].Pechenkina and coworkers in 2002 studied bone exostosisdevelopment in 66 well-preserved crania from NeolithicChina to establish a possible etiopathological association[7]. The role of ethnic and genetic predispositions seemedto be important in the occurrence of such exostoses. In thesame study, authors considered other factors as potentiallyrelated to exostosis formation such as masticatory hyper-function (clenching or bruxism), occlusal stress, a compensa-tory response of periodontal disease, and chemical agentsand concluded that every periodontal microdamage orinflammation can evolve in exostoses in genetically predis-posed subjects. Severe occlusal stress, associated with tempo-romandibular joint disorders, was hypothesized to be a majorenvironmental factor predisposing to exostosis development[7]. Echeverria and colleagues in 2001 presented a reportabout the formation of exostoses following FGG surgicalprocedure [8]. The authors postulated that surgical traumacombined with occlusal stress and genetic factors could causethe occurrence of exostoses. They also noticed that most ofthe post-FGG exostoses appeared in the premolar area, prob-ably due to excessive chewing forces. The histological exam-ination of hard and soft tissues of exostoses showed denseand mature lamellar bone surrounded by fibrous tissue withthe presence of acanthosis, pseudoepitheliomatous hyperpla-sia, parakeratosis, and reactive fibrosis [8]. Likewise, otherauthors proposed the same potential etiological explanation,suggesting that the periodontal trauma and surgical damageof soft tissue (plastic surgery of the vestibule, gingival graft,and, notably, fenestration occurred in the periosteum) canevolve in buccal bony exostoses in genetically predisposedpatients [3, 9–14].
Since the mechanisms leading to the overgrowth of gingi-val connective tissue overlying the exostosis are notcompletely known, the aim of this study was to characterizethe gingival connective tissue in the case of multiple exosto-ses following FGG by histological and molecular analysis.
2. Case Presentation
2.1. Patient. A female patient, 34 years old at the time of thefirst visit, was referred to us to correct her gingival estheticproblem caused by the formation of gingival/mucosal over-growths. Gingival outgrowths localized in the second, third,and fourth quadrants, corresponding to the position of pre-molars and canine roots, were identified during an oralexamination. Their consistency appeared hard and unmov-able, and they presented a normal gingival color. The lesionswere asymptomatic, and the morphology did not change overtime (Figure 1).
The anamnestic investigation revealed a history of FGGsurgery in 2004 (8 years before our first examination) in eachsite that was treated for gingival recessions; allergic reactionsto nonsteroidal anti-inflammatory drugs, paracetamol, ceph-alosporin, penicillin and its derivatives, and macrolide
antibiotics; absence of any systemic conditions affectingmetabolism; and altered wound healing (cutaneous keloidsand hypertrophic scars).
The hypothesis of exostosis formation was suggested bythe following clinical observations: benign aspect, nodularand hyperplastic tissue, hard and stable on palpation, notsymptomatic, and localized in canine and premolar areasinvolved in a surgical procedure that could have acted as apromoter event.
We decided to perform an excisional surgery, followed bya histologic and molecular evaluation. The Review Board ofthe Research Center in Oral Implantology of the Universitàdegli Studi di Milano in Milan, Italy, approved the treatmentand research protocol before the first surgery.
All procedures were in accordance with the ethical stan-dards of the institutional and/or national research commit-tee and with the 1964 Helsinki declaration and its lateramendments or comparable ethical standards. The patientwas informed about the formulated medical hypothesisand the need for surgical intervention to remove the lesionsand analyze the specimen. The patient agreed to the surgeryand signed a written informed consent form. The surgerywas planned after a complete evaluation of the patient’ssystemic conditions that confirmed an absence of clinicalcontraindications.
2.2. Surgical Procedures. All surgeries were performed inthree different sessions by the same operator, with more thanten years of experience in the field of oral surgery (LF), andfollowing the same protocol. The first surgical event involveda lesion localized in the second quadrant. Local anesthesiawas performed with articaine 4% + epinephrine 1 : 100.000,voiding injection in the lesion area. The lesion was first iso-lated by incisions with a microblade following its externalborder; after that, the incision was continued above the peri-osteum, removing the tissue covering the exostosis. Then,with a 15C blade, a full-thickness incision was performedcoronally to the lesion and the exostosis surface was exposedusing a periosteal elevator. The full-thickness incision wasextended in the mesiodistal direction in order to have a betterview of the surgical site. Gingival fragments obtained fromthe overgrown gingiva were collected for histological andmolecular characterization. A carbide bur, mounted on arotary instrument and under sterile saline solution irrigation,was used to remove the bone structure in order to recreate aplane surface. To obtain a primary closure of the flap after the
Figure 1: Clinical aspect at baseline.
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removal of the lesion, a partial thickness incision was per-formed in the free alveolar mucosa. The flap was then suturedwith a 6/0 nonresorbable suture (ETHILON™ Nylon Suture,Ethicon ®, Cincinnati, OH, United States). After flap clo-sure, an infiltration of 4 mg corticosteroid (BENTELAN4 mg/2 mL, Defiante Farmaceutica S.A., Madeira, Portugal)was performed, to prevent the recurrence of overgrowths inthe short term. The last surgical procedure was performedwithin 4 months of the first visit.
The follow-up visits revealed small relapses after 18months from the surgical intervention that were asymptom-atic and did not affect aesthetics and function. The recur-rences observed in the second and fourth quadrants werelinearly arranged along the incision of the periosteum thatoccurred during the surgery, while the lesion treated in thethird quadrant showed a more evident relapse.
2.3. Histological Analysis. One gingival fragment was proc-essed for histological analysis while another fragment wascultured for in vitro evaluations. Moreover, to characterizethe pathological gingiva, a fragment from the healthy papillawas obtained from the same patient for comparison.
Immediately after surgery, each tissue fragment was fixedin 4% formalin in 0.1 M phosphate-buffered saline (PBS), pH7.4, routinely dehydrated and paraffin-embedded. Serial sec-tions of 5 μm were obtained. Sections were stained withfreshly made hematoxylin-eosin (Sigma-Aldrich, St Louis,MO, United States) to evaluate cell and tissue morphology.
To evaluate fibrillary collagen, slides were deparaffinizedand immersed for 30 minutes in saturated aqueous picricacid containing 0.1% Sirius Red F3BA (Sigma-Aldrich, St.Louis, MO, United States), a staining method that specifi-cally marks collagen [15]. The sections were observedunder a light and polarized microscope (Nikon EclipseE600, Nikon, Tokyo, Japan) and photographed with a cal-ibrated digital camera (DXM1200, Nikon, Tokyo, Japan) ata 20x magnification.
2.4. Cell Cultures. Human gingival fibroblasts were obtainedfrom a gingival fragment covering the exostosis and from agingival fragment obtained from the patient’s healthy gin-giva. Tissue fragments were washed with sterile PBS, platedin T25 flasks, and incubated in DMEM containing 10%heat-inactivated fetal bovine serum (FBS) and antibiotics(100 U/mL penicillin, 0.1 mg/mL streptomycin) at 37°C ina humidified atmosphere with 5% CO2. Fibroblasts that grewout from the explant were trypsinized (0.025% trypsin-0.01%EDTA) for secondary cultures and plated in T75 flasks, aspreviously described [16, 17]. Human gingival fibroblastswere used between the fourth and fifth passages. Molecularevaluations were performed in samples cultured for 24, 48,and 72 h. All evaluations were performed on duplicate cul-tures for each sample.
2.5. Cell Morphology and Viability. Cell morphology ofcultured gingival fibroblasts was analyzed by phase-contrast microscopy using a Leica DM IL microscope. Cellviability was determined by Trypan blue exclusion. Growthcurves were obtained after plating cells in 6-well plates
(150,000 cells/well). After the attachment, the cells werecounted after 24, 48, and 72 h.
2.6. Real-Time RT-PCR. Total RNA was isolated from cul-tured fibroblasts as previously described [17]. Briefly, 1 μgof total RNA was reverse-transcribed in a 20 μL final volumeof reaction mix (Bio-Rad, Segrate, Milan, Italy). Gene expres-sion for the tissue inhibitor of matrix metalloproteinase 1(TIMP-1), lysyl oxidase (LOX), and focal adhesion kinase(FAK) was assessed. GAPDH was used as internal controlfor normalization of the differences in the amount of totalRNA in each sample. The following primers were used:GAPDH: sense CCCTTCATTGACCTCAACTACATG,antisense TGGGATTTCCATTGATGACAAGC, antisenseGGAACCAGGATGACCAGATGTACC; TIMP-1: senseGGCTTCTGGCATCCTGTTGTTG, antisense AAGGTGGTCTGGTTGACTTCTGG; LOX: sense GGATACGGCACTGGCTACTT, antisense GACGCCTGGATGTAGTAGGG;and FAK: sense GTCTGCCTTCGCTTCACG, antisenseGAATTTGTAACTGGAAGATGCAAG.
Amplification was performed in a 96-well plate in a finalvolume of 20 μL per well containing 10 μL of 1x SYBR GreenSupermix (Bio-Rad, Italy), 2 μL of template, and 300 pmolof each primer, and each sample was analyzed in triplicatein an iQ5 thermal cycler (Bio-Rad, Italy) after 40 cycles.After determining the cycle threshold (Ct), gene expres-sion levels relative to that of GAPDH were calculated bythe 2ΔCt method.
2.7. Slot Blot. Slot blot analysis was used to assess type I col-lagen (COL-I), type III collagen (COL-III), and matrixmetalloproteinase-1 (MMP-1) protein levels secreted by gin-gival fibroblasts in cell culture supernatants, as previouslydescribed [17]. Briefly, protein content in cell culture mediawas determined by a colorimetric assay (DC Protein Assay,Bio-Rad, Italy); 100 μg of total proteins per sample (final vol-ume of 200 μL of Tris-buffered saline (TBS)) was spottedonto a nitrocellulose membrane using a Bio-Dot SF appara-tus (Bio-Rad, Italy). After blocking for 1 h with 5% skimmedmilk in TBST (TBS containing 0.05% Tween-20), pH 8,membranes were incubated for 1 h at room temperature ina monoclonal antibody to COL-I (1:1000 in TBST) (Sigma,Italy), to COL-III (1:2000 in TBST) (Sigma, Italy) and toMMP-1 (1 μg/ml in TBST, Millipore). After washing withTBST, membranes were incubated in HRP-conjugated rabbitanti-mouse serum for 1 h (1 : 6000 in TBST to detect COL-Iand COL-III or 1 : 40,000 in TBST to detect MMP-1, respec-tively) (Sigma, Italy). Immunoreactive bands were revealedby the Opti-4CN substrate or Amplified Opti-4CN substrate(Bio-Rad, Italy) and quantified by densitometric scanning(UVBand, Eppendorf, Italy).
2.8. SDS-Zymography. Serum-free cell culture media weremixed 3 : 1 with sample buffer (containing 10% SDS), aspreviously described [16, 17]. Five μg of total protein persample was run under nonreducing conditions without heatdenaturation on 10% polyacrylamide gel (SDS-PAGE)copolymerized with 1 mg/mL of type I gelatin at 4°C. AfterSDS-PAGE, the gels were washed twice in a renaturing buffer
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(2.5% Triton X-100, Tris-HCl 50 mM) for 30 min each andincubated overnight in a substrate buffer at 37°C (Tris-HCl50 mM, CaCl2 5 mM, NaN3 0.02%, pH 7.5). After stainingthe gels with Coomassie Brilliant Blue R-250, MMP gelatino-lytic activity was detected as clear bands on a blue back-ground. Bands were quantified by densitometric scanning(UVBand, Eppendorf, Italy).
2.9. Statistical Analysis. Data, expressed by mean ± standarddeviation (SD), were analyzed by t-test. p values less than0.05 were considered significant.
3. Results
3.1. Clinical Outcomes. From a clinical point of view, in thelast follow-up visit, after 4 years from the last surgical inter-vention, no exostosis or gingival overgrowth formationscould be observed (Figure 2) and we recorded a high degreeof satisfaction with the treatment by the patient. However,scars, where surgeries were performed, were visible and
clearly distinguishable from the surrounding tissues. Palpa-tion did not reveal any overgrowth of soft or hard tissue.
3.2. Histological Analysis. Light microscopy analysis ofhematoxylin and eosin-stained samples revealed that tissueintegrity and structure were maintained in healthy andovergrown tissues (Figures 3(a) and 3(b)). The liningsquamous-stratified epithelium was orthokeratinized, dis-playing a normal structure and normal thickness of thestratum corneum in both healthy and overgrown tissues.The dense irregular connective tissue was characterized bywell-organized collagen fibers and an extracellular matrixwith blood vessels normally distributed and without anyinflammatory infiltrates. Sirius red staining specificallystained collagen fibers, densely packed in an irregular array,and revealed that the healthy and overgrown gingivae dis-played a similar collagen content and arrangement(Figures 3(c) and 3(d)). The presence of connective papillaryprojections throughout the epithelium was also evident. Theanalysis of Sirius Red-stained sections under polarized light
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Figure 2: Clinical situation 4 years after the last surgical intervention. In the absence of gingival overgrowth, formation of thin scars is visible.
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allowed detecting some qualitative differences in the epithe-lium and in the lamina propria of the healthy and overgrowngingivae. The healthy epithelium was totally lacking birefrin-gence, while the overgrown gingiva showed a thin and notuniform birefringent layer in the superficial portion. Theconnective tissue was also analyzed under polarized light.
According to Junqueira et al., under polarized light, the colla-gen matrix is stained from green to yellow to orange-reddepending on the orientation of the collagen bundles andthe maturation of collagen [15]. Specifically, the newlysecreted and less mature collagen is stained in green whilethe mature collagen is stained in orange-red. Healthy gingiva
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Figure 3: Micrographs showing the healthy and overgrown gingivae. (a-b) Hematoxylin and eosin staining reveals the morphologicalstructure of the epithelium and of the connective tissue. (c-d) Sirius Red staining stains in red the collagen fibers of the connective tissue.(e-f) Polarized light performed on Sirius Red-stained samples shows birefringence relative to collagen fiber arrangement and maturation.Original magnification: 20x.
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appeared red under polarized light (Figure 3(e)). By contrast,red birefringence was less evident in the overgrown gingivaand the connective tissue was stained green to yellow, sug-gesting that the collagen was less mature (Figure 3(f)).
3.3. Cell Viability. Growth curves have shown that fibroblastsfrom the healthy and overgrowth gingivae exhibited a differ-ent proliferation rate at the considered time points. Fibro-blasts from the overgrown gingiva tended to increaseproliferation after 24, 48, and 72 h (Figure 4).
3.4. Collagen Turnover. The histological findings were sup-ported by the analysis of collagen turnover. Collagen contentin gingival fibroblast supernatants revealed that fibroblastsfrom the overgrown gingiva synthesized and secreted higherCOL-I (p < 0, 01, p < 0, 05, and p = 0,066 vs. healthy gingivaafter 24, 48, and 72 h, respectively) and COL-III proteinlevels (p = 0, 08, p = 0, 09, and p ns vs. healthy gingiva after24, 48, and 72 h, respectively) (Figures 5(a) and 5(b)).
Gene expression for LOX resulted to be strongly upregu-lated in fibroblasts from the overgrown gingiva (p < 0 005 vs.healthy at 72 h) (Figure 5(c)).
Collagen degradation pathways were assayed by slot blot,real-time PCR, and SDS-zymography. MMP-1 levels tendedto increase in fibroblasts obtained from the overgrown gin-giva (p < 0, 05, p = 0, 06, and p = 0,073 vs. healthy gingivaafter 24, 48, and 72 h, respectively) (Figures 6(a) and 6(b)).The expression of TIMP-1 mRNA, the main inhibitor ofMMP-1, was unchanged after 24 and 48 h but stronglyincreased in fibroblasts from the overgrown gingiva after 72h (p < 0,001) (Figure 6(c)). MMP-2 activity was characterizedby a pattern concordant withMMP-1 protein levels (p < 0, 05,p < 0, 05, and p ns vs. healthy gingiva after 24, 48, and 72 h,respectively) (Figures 7(a) and 7(b)).
3.5. FAK Gene Expression. Gene expression analysis for FAKrevealed similar mRNA levels in both experimental condi-tions after 24 and 48 h and a tendency towards upregulationin fibroblasts from the overgrown gingiva after 72 h(p = 0 066) (Figure 8).
4. Discussion
Alteration of the oral mucosa wounding affecting this patientsuggests a possible relationship between keloid developmentand exostosis formation. To evaluate this association, wedecided to analyze the healthy and overgrown gingivae over-lying the exostosis of the same patient. According to the clin-ical observations, traumatism produced during the surgeryseems to be relevant to the occurrence of relapses [1, 3].
Histological analysis revealed that the epithelium of theovergrown gingiva exhibited a normal general structure butshowed an evident birefringence of the stratum corneum.We hypothesize that the mechanical stimulus exerted bythe exostosis on the overlying gingiva resulted in a morecompact stratum corneum, containing dense birefringentkeratin bundles.
Collagen is the main component of the gingival denseirregular connective tissue. The histological analysis of thehealthy and overgrown gingivae revealed a similar collagencontent, as shown by Sirius Red staining. Polarized lightmicroscopy revealed qualitative differences in collagen con-tent, as the overgrown gingiva presented green-yellow color-ation, while the healthy gingiva was stained in red, indicatingthat the collagen in the overgrown gingiva is less mature andtherefore more susceptible to degradation by MMPs.
In order to understand the mechanisms responsible forthis difference, we analyzed collagen turnover pathways incultured gingival fibroblasts from the healthy and overgrowngingivae at the molecular level.
Collagen content is determined by the finely regulateddynamic balance between its synthesis and degradation byMMPs. MMP-1 begins collagen degradation breaking downthe native triple helical region of interstitial collagens intocharacteristic 3/4- and 1/4-collagen degradation fragments,also known as gelatins [18], that can be further degraded tocomplete the digestion by less specific proteinases such asMMP-2 [19, 20]. MMP activity is regulated at the posttrans-lational level by TIMPs, inhibiting their activation and theiractivity [21]. Our results show that COL-I, the main compo-nent of gingival connective tissue, and COL-III protein levelswere increased in supernatants of gingival fibroblasts fromthe overgrown gingiva. Moreover, an increased proliferationof fibroblasts obtained from the overgrown gingiva wasobserved. The apparent inconsistency between the morpho-logical analysis, showing a similar collagen content, andincreased collagen protein levels secreted by cultured fibro-blasts obtained from the overgrown gingiva can be explainedby analyzing the collagen degradation pathways. In fact, theobserved concomitant increased MMP-1 and MMP-2protein levels suggest an increased collagen turnover rate infibroblasts from the overgrown gingiva. Consequently,increased collagen secretion in the overgrown gingiva isconcomitant and paralleled with the increase in its degra-dation, resulting in a similar collagen content at thesteady state in the connective tissue, as observed at thehistological level. According to this suggestion, we canhypothesize that the observed TIMP-1 upregulation canbe induced as a response to the MMP-1 overexpression,in order to balance it.
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Figure 4: Cell proliferation. Growth curves showing cell viabilityin fibroblasts from healthy and overgrown gingivae after 24, 48,and 72 h.
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Figure 5: Collagen expression and maturation. Bar graphs showing COL-I (a) and COL-III (b) protein levels assessed by slot blot in cellculture supernatants of fibroblasts from healthy and overgrown gingivae. (c) Bar graphs showing LOX mRNA levels in fibroblasts fromhealthy and overgrown gingivae. GAPDH gene expression was used as internal control for normalization. Data are mean ± SD for samplesrun in duplicate. ∗p < 0 01, ∗∗p < 0 05, and ∗∗∗p < 0 005.
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Figure 6: MMP-1 and TIMP-1 expression. (a) Representative slot blot showing MMP-1 levels in serum-free cell culture supernatants offibroblasts from healthy and overgrown gingivae. Immunoreactive bands were quantified by densitometric scanning as described inMaterials and Methods. (b) Bar graphs showing MMP-1 protein levels analyzed by slot blot. Data are expressed as densitometric units andare mean ± SD for samples run in duplicate. (c) Bar graphs showing TIMP-1 gene expression after normalization on GAPDH mRNAlevels. Data are expressed as mean ± SD for samples run in triplicate. ∗p < 0 05 and ∗∗p < 0 001.
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Figure 7: MMP-2 activity. (a) Representative SDS-zymography and (b) bar graphs showing MMP-2 activity in serum-free cell culturesupernatants of fibroblasts from healthy and overgrown gingivae after densitometric analysis of immunoreactive and lytic bands. Data areexpressed as densitometric units and are mean ± SD for samples run in duplicate. ∗p < 0 05.
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Newly synthesized collagen undergoes cross-linking, animportant requirement for collagen maturation that providescollagen fibril stabilization and an increased tensile strength[22, 23]. Here, we show that LOX is strongly and significantlyincreased in fibroblasts from the overgrown gingiva, pointingto an increased collagen cross-linking activity induced bythe exostosis.
The increased collagen turnover in fibroblasts from theovergrown gingiva could be therefore responsible for the dif-ferences revealed by the histological analysis. In fact, theexostosis could trigger a more rapid collagen turnover, con-sistent with the qualitative differences observed by polarizedmicroscopy. However, in fibroblasts from the overgrown gin-giva, LOX is significantly induced, suggesting that the newlysecreted collagen in overgrown gingiva could undergo anincreased cross-linking and maturation. Overexpression ofLOX is very likely an adaptive response to avoid an excesscollagen degradation in the gingiva, and it could favor col-lagen deposition in the gingival connective tissue coveringthe exostosis.
Collagen turnover rates can be affected by drugs as well asmechanical stimulation. Previous studies have shown thatmechanical loading stimulates extracellular matrix (ECM)protein production by promoting the release of growth fac-tors, such as TGF-β1, FGF, and PDGF [24]. Mechanical load-ing of cells has also been shown to modulate ECM turnoverby regulating the expression and activity of MMPs [25–27].Finally, mechanical loading interacts with growth factorsand cytokines to regulate ECM homeostasis in various tis-sues [27–31]. We feel that our hypothesis of the role ofexostosis as a triggering stimulus that influences gingivalfibroblast activity is strengthened by the observations ofgingival overgrowth exclusive to exostosis sites. Multipleexostoses were observed, each one covered by a gingivalovergrowth lesion.
These suggestions are consistent with the tensegritymodel described by Ingber, referring to a model of tensionalintegrity based on a network of structures that mechanicallystabilize themselves using tensile prestress [32, 33].According to this model, the whole structure is placed ina state of isometric tension that makes it strong, resilient,and immediately responsive to external mechanical stresses.
The tensegrity model, referred to cells, led to the concept thatmechanical forces would be sensed by cells using their cyto-skeleton. In fact, a connection between cytoskeleton in thecell cytoplasm and the surrounding ECM is provided by thetransmembrane proteins, integrins, working as mechanore-ceptors, and finds its most useful application in understand-ing cellular mechanotransduction [34]. Integrins, mediatingthe attachment of cells to the ECM, are transmembrane pro-teins that provide a bridge through which forces can be trans-mitted between the inside and the outside of the cells. In fact,their extracellular domain binds to ECM components,whereas its cytoplasmic domain links various intracellularproteins interposed between actin filaments and integrin,forming focal adhesion complexes [35]. Mechanotransduc-tion, the process that transforms mechanical stimuli intochemical signals, involves mechanosensory units integratedin the cell membrane that transduce mechanical signals intoa biochemical response through force-dependent changes,important for regulation of global functions of the cell. Ourdata, showing a tendency to an increased gene expression ofFAK, one of the components of the focal adhesions acting asa mechanoresponsive apparatus, is consistent with thishypothesis. In fact, an increase in focal adhesions as a responseto mechanical stimulation elicited by the exostoses could beconsidered an adaptive response of gingival fibroblasts.
According to this model, we can consider gingivalfibroblasts as cells using tensegrity architecture for theirorganization. This suggestion is supported by several stud-ies that investigated the expression of ECM proteins infibroblasts embedded in collagen gels under stretched andrelaxed conditions, showing that the fibroblasts in the colla-gen adopted a synthetic phenotype characterized by theirability to synthesize matrix proteins and inhibit matrixdegradation [36].
In this study, only one case of gingival overgrowth hasbeen examined and we are aware that additional cases couldadvance and confirm our hypotheses; unfortunately, cases ofmultiple exostoses are very infrequent and this is the onlycase that came to our attention to date. Nevertheless, theassociation between exostoses and gingival overgrowth isstrengthened by the presence of the overgrowths exclusivelyin the connective tissue covering each exostosis.
Our hypothesis is consistent with data previouslyreported [1, 3], showing a relationship between exostosisand FGG; these studies suggest that exostosis could be a rareside effect of FGG as a consequence of the periosteal trauma-tism during site preparation that induces the activation ofosteoprogenitor cells. The clinical history of the patientrevealed that the exostosis was formed after a surgical proce-dure that, very likely, triggered this mechanism inducing theexostosis, leading to the gingival overgrowth (Figure 9).
Our results suggest that exostosis could represent amechanical stimulus stretching the overlying connective tis-sue and triggering a synthetic phenotype in gingival fibro-blasts that, in turn, increase their activity in collagenturnover and maturation pathways. The effect of this stimu-lation seems responsible for the qualitative differencesobserved in the gingival connective tissue, thus affecting itsbiological and biomechanical properties. From a clinical
Rela
tive e
xpre
ssio
nFAK mRNA
0.000
0.002
0.006
0.009
0.012
24 h 48 h 72 h
HealthyOvergrowth
Figure 8: FAK mRNA levels. Bar graphs showing FAK mRNAlevels after normalization on GAPDH gene expression. Data areexpressed as mean ± SD for samples run in triplicate.
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point of view, the results of this study suggest that the surgicalremoval of such lesions, which is the only possible treatment,should be performed using minimally invasive surgicaltechniques to avoid traumatizing the periosteal tissues andtriggering the mechanisms that can lead to gingival over-growth. This procedure can be successful in the eradicationof the exostosis with minimal relapses. A cautious use ofdrugs interfering with gingival collagen turnovers, such ascyclosporine A, anticonvulsants, and calcium antagonists isalso advised.
Conflicts of Interest
The authors declare that there is no conflict of interestregarding the publication of this article.
Authors’ Contributions
Luca Francetti and Claudia Dellavia contributed equally tothis work.
Acknowledgments
We thank Nataliya Khomchyna for the English editing ofthe manuscript.
References
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