Microbiology Overview

KWWOA

April 09, 2014

Department for Environmental ProtectionEnergy & Environment Cabinet

Topics

Topics

o Proficiency Tests

o Chain of Custody

o Methods

o Membrane Filtration

Proficiency Tests

Proficiency Tests

o Drinking Water: WS StudySW sample for MPN

o Wastewater: DMR-QA WP Study

o KY Approved PT Providers List

Proficiency Tests

Proficiency Tests

Proficiency Tests

Proficiency Tests

Revised reports will not be accepted.

Photo: peoplesdaily.com

Chain of Custody

Chain of Custody

• Client name & address

• Sampler name

• Sample location

• Collection date & time

• Sample type

(grab/composite)

• Matrix (DW/WW)

• Field sample ID

• Laboratory sample ID

• Container type, & size/volume

• Number of containers

• Preservative (ice, etc.)

• Field data (pH, TRC, etc.)

• Requested analysis

• Reason for rejection or

special remarks

• Signature, date & time for

each transaction

(relinquishing & receiving)

• Sample temperature

upon arrival at lab

BARF is NOT a COC

Methods

Microbiology Methods

Method Drinking Water Wastewater

Colilert E. coli

Colilert-18 E. coliFecal – ATP @ 44.5oC

Colisure

Coliscan

mFC NA-MUG Fecal

mEndo EC-MUG

mColiBlue-24 E. coli

Modified mTEC E. coli

17

Colilert-18 P/A

Colilert-18 pre-warming

18

Colilert-18 MPN

Membrane Filtration

Membrane Filtration

Dilutions:

Select appropriate volumes

Fecal Coliform or E. coli colonies: 20-60*

Total colonies: 200*Use range as specified in particular method.

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Membrane Filtration

Blanks:• Beginning of series

• End of series

• RECORD results of blanks

Membrane Filtration

Calculating Results:• Use dilution that is in range to

determine result.

• If multiple plates within range, average those plate counts.

• If zero colonies on all plates, calculate as if 1 colony on filter from largest volume processed. Include remark “<“ in comments.

Photos: www.avocetlab.com

Membrane Filtration

Calculating Results:

• If all plates are above range, calculate using filter from smallest volume processed. Include remark “Estimated“ in comments.

• WW TNTC = 60,000

Contact Information

Tiffany Nolan

Kentucky Department for Environmental ProtectionDivision of Water

200 Fair Oaks Lane; 4th FloorFrankfort, KY 40601

Phone: (502) 564-3410

Email: Tiffany.Nolan@ky.gov