Intravascular nevus cell protrusion

and aggregates in otherwise

common melanocytic nevi

Georgiana-Irina Tudor 1 Oana Stefan1, Victor Nica1, RalucaTanasa1, Virginia Chitu1,2, Constantin Caruntu2, Cristiana Popp1,

Mirela Cioplea1 and Sabina Zurac1,2

1 Colentina University Hospital 2 “Carol Davila” University of Medicine and Pharmacy

Bucharest, Romania

♀, 36 yrs

abdomen

Intradermal nevus H&E, 100x

Intravascular nevus cell protrusion (IVNcP)

H&E, 400x CD 34, 400x

Intravascular nevus cell protrusion (IVNcP)

D2-40, 100x D2-40, 400x

Intravascular nevus cell

aggregates (IVNcA)

H&E, 200x CD 34, 400x

Study methods

Cross-sectional study

1154 benign cutaneous compound and intradermal nevi

January 2017- December 2018

Colentina University Hospital, Pathology Department

Study methods

Inclusion criteria:

-presence of intravascular nevus cell protrusions & intravascular nevus

cell aggregates (H&E, 10x objective), confirmed with

immunohistochemical stainings with S100, CD34, D2-40

Exclusion criteria-presence of the pseudovascular spaces

Study methods

80 nevi with vascular affinity

Demographic data

Anatomical region

Diameter, maximum thickness, dermal level of vascular interest

Results

78; 97%

2; 3%

Intravascular nevus cell protrusion and aggregates distribution

IVNcP

IVNcA+IVNcP

100% with

intravascular

nevus cell

protrusion!

Results

23; 29%

57; 71%

Gender ratio

Male

Female

Female:Male=2.5:1

Results

0

5

10

15

20

25

30

≤20 21-30 31-40 41-50 51-60 61-70 71-80

8

2623

16

2 3 2

Age interval

Age distribution

Median age 32 yrs (95% CI 16-80)

Results

30%

10%

1%

59%

Anatomical location

head and neck

unknown

limbs

trunk

Median diameter 0.7 cm

(95% CI 0.2-2.3)

Median of maximum tumor

thickness 0.31 cm (95% CI

0.07-0.9)

Level of dermal nevus cell

protrusion and aggregates Results

Upper dermis

Central/later part of the nevi

Discussions

Intravascular nevus cell protrusion and aggregates: ◦ young female

◦ small, thin lesion

◦ trunk/head and neck region

Discussions

Neural crest migration and mechanical transport theories

IVNcA IVNcP

(HE) (IHC)

Nodal melanocytic nevi

IVNcA (H&E, 400x) IVNcP (S100/CD34, 200x)

Limitations Additional sections

Incomplete clinical data (congenital/acquired)

Mechanisms that explain nevus cell vascular affinity?

This work was supported by a grant of Romanian-

Ministry-of-Research-and-Innovation, CCCDI-

UEFISCDI, project number 61PCCDI⁄2018PN-III-

P1-1.2-PCCDI-2017-0341.

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Thank you!