Acetic Acid-Induced Writhing Test. This test was conducted

according to the description published by Koster et

al. [24]. The presence of abdominal contortions induced by

an intraperitoneal injection of acetic acid was considered

nociceptive behavior, characterized by abdominal muscle

contractions followed by stretching of the hind limbs [25,

26]. For this experiment, groups of 8 mice received the

following types of pretreatment: vehicle, phytol at the doses

of 25, 50, 100, and 200mg/kg, indomethacin (10mg/kg),

or morphine (10mg/kg). Thirty minutes after pretreatment,

the animals received intraperitoneal injections of a 0.85%

solution of acetic acid (0.1 mL