Genetics and Breeding for ALS resistance in common...
Transcript of Genetics and Breeding for ALS resistance in common...
Genetics and Breeding for ALS resistance in common bean
E.A. Souza A.F.B. Abreu
M.A.P. Ramalho R. Pereira
S. Librelon
Common Bean Disease Workshop on Angular Leaf Spot and Root Rots
UFLA MG
6347
Lavras
INTRODUCTION
Common bean is grown in three different seasons in Brazil:
Dry season (February-March)
Winter season (May-July)
Wet season (October-November)
Area: 3.990.000 ha
Source: Conab/IBGE
Common bean production in 2013/2014
INTRODUCTION • Common bean breeding program of UFLA
– Yield and grain type – Plant architecture – Nutrition quality – Efficiency in water use – Resistance to diseases: ALS, anthracnose and white
mold
Magno A.P. Ramalho Ângela F.B.Abreu João B. Santos
Experimental field of UFLA, Lavras, MG, Brazil
INTRODUCTION
• Angular leaf spot– Pseudocercospora griseola – System of race identification (Pastor-Corrales e Jara 1995)
– Most frequent race– 63-63 (Damasceno-Silva et al. 2008, Balbi et al. 2009, Pereira et al. 2015)
– Variability within race 63-63 (Pereira et al. , 2015)
Wide variability has been observed in populations of P. griseola from Brazil by molecular markers, hyphal anastomosis and virulence.
Silva et al. 2015, Genet. Mol. Res.
Methodology for inoculation of P. griseola in primary leaves of common bean (Pereira et al. 2011)
Resistant- score ≤ 3 Susceptible – scores ≥ 3
Leaf-dextrose-agar media 7 days at 24 °C in the dark
2 x 104 spores/ml
9 seeds/line and two replications (trays)
PDA Media
15 dai – evaluation of ALS severity Scale 1 to 9
Rosinha – susceptible control
Greenhouse with 24 °C and 90% humidity
Librelon et al. 2015, Australasian Plant Pathol.
Diagrammatic scale for evaluation of ALS severity in primary leaves of common bean
A-285 CNF 243 ESAL 501 ESAL 553 ESAL 620 ESAL 683 Jalo-6 MAV-3-36 TU X CARIOCA
A-767 CNF 246 ESAL 502 ESAL 562 ESAL 630 FEIJAO BAGAJO LP 806 MAVI-21* UTF 0037
AN 910522 CNF 252 ESAL 503 ESAL 565 ESAL 631 H-4-12 LP 98-31 MAVI-39
VERME LHO G-2
ANPAT 8.12 CNF 255 ESAL 505 ESAL 574 ESAL 632 H-4-18 LP-808
MULATI NHO 106 VP-20
AROANA CNFC 10764 ESAL 512 ESAL 590 ESAL 635 HULK
LR 720982 CP NEGRITO VP-23
BOLINHA CNFP 7726 ESAL 516 ESAL 593 ESAL 640 IAPAR 20
LR 9115302
OPNS/VC3-177
BP-24 CNFP 7966 ESAL 521 ESAL 595 ESAL 648 ICA PIJAO
LR 9115315 P5-9
BP-28 CORNELL ESAL 523 ESAL 606 ESAL 651 JAIME 155 LR 9115332 PARANA
CARIOCA TU
COSTA RICA 1031 ESAL 533 ESAL 608 ESAL 652 JAIME 182 MAII-10
PF 9029984
CI-107-6 CV-55 ESAL 537 ESAL 609 ESAL 657 JAIME 196 MAII-14 R-29
CI-257-1 CVII-45.5 ESAL 543 ESAL 612 ESAL 660 JALO MAIV-18-264
RIO TIBAGI
CIII-H-4-12
ERIPAR SA 1 ESAL 550 ESAL 613 ESAL 664 JALO EEP MAV-1-7 TO
Resistant common bean lines to race 63-63* of P. griseola from Germplasm Bank of the UFLA * one isolate, unpublished data (467 common bean lines evaluated and 101 resistant)
* Common bean lines from ALS recurrent selection program UFLA/Embrapa
Evaluation of ALS severity in common bean lines from UFLA Germoplasm Bank
Symptom of ALS in primary leaves of common bean
Scores of ALS severity of common bean cultivars and compatibility reaction when inoculated with isolates P.griseola.
Isolates Cultivars
Ouro Negro Pérola MA III Madrepérola 1 3,3S* 3,4S 2,3R 4,6S 2 7,2S 7,7S 2,6R 7,1S 3 5,5S 6,8S 2,9R 5,9S 4 3,4S 3,8S 4,4S 4,3S 5 6,6S 8,1S 4,1S 7,3S 6 5,5S 6,4S 2,1R 5,3S 7 5,0S 5,1S 3,1S 4,2S 8 4,4S 5,1S 1,4R 4,5S 9 5,4S 6,3S 2,9R 5,5S
10 6,8S 7,3S 2,0R 6,3S 11 4,5S 6,0S 1,9R 4,7S 12 5,9S 6,7S 2,7R 5,7S
•Scores ≤ 3 – resistant and ≥3 susceptible •Inoculation in V2 stage
Recurrent Selection for Resistance to ALS
Lines with grain type Carioca X
Resistant lines 1999
↓ F1 ↓
Base Population (F2=S0 C0) Phenotypic seletion of plants ↓
Intercrossing of more resistant plants for obtention CI
↓ S0 of CI Phenotypic seletion of plants
S0:1 Intercrossing of more resistant plants for obtention
CII
2000
↓ 2000 F1of CII
↓ Lines S0 of CII Phenotypic seletion of
plants 2001
Repeat the process
S0 of CXIII Phenotypic seletion of plants
2013
S1 do CXIII
Intercrossing of more plants for obtention CXIV
2013
↓ ↓ S2 of CXIII
F1of CXIV 2013
↓ ↓ S3 of CXIII
S0 of CXIV Phenotypic seletion of plants
2014
↓ Seletion of progenies for evaluation in generations S3:4, S3:5 and S3:6
Repeat the process
Recurrent selection for ALS using artificial inoculation
(1) Inoculation in primary leaves in greenhouse
(2) Selection and transplant of
resistant plants for pots
(3) Intercrossing of resistant
plants (4)
Sowing and inoculation of F1 plants
It is possible to carry out three cicles for year!
Humidity and temperature control
Analysis of resistance and aggressiveness in common bean-P. griseola pathosystem
Pereira et al. 2015, Genet. Mol. Res.
Pereira et al. 2015, Genet. Mol. Res.
Association mapping of loci related to angular leaf spot resistance
• Phenotypic evaluation -greenhouse and field • An association panel comprising 144 common bean lines
Genotypic evaluation -250 SNPs Project UFLA/Embrapa
Rafael Pereira
Samira Librelon
Kaesel Jackson Damasceno e Silva
Paula Furtado de Pádua
Luanna B. W. Gomes
Cassius N. Souza Preire
Quélen de Lima Barcelos
Thank you!