Chapter 3 Lecture Concepts of Genetics Tenth Edition Mendelian Genetics.
General Genetics Assignment 3 Question 3
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Transcript of General Genetics Assignment 3 Question 3
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8/19/2019 General Genetics Assignment 3 Question 3
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3. A piece of DNA 900 bp long is cloned and then cut out of a vector for analysis.
Digestion of this linear piece of DNA with three different restriction enzymes singly
and in all possible pairs of enzymes gave the following restriction fragment size data.
Construct a restriction map from these data.
nzymes !estriction "ragment #izes Eco !$ %00 bp& '00 bp Hin d$$$ 300 bp& (00 bp Bam )$ *0 bp& 3*0 bp& *00 bp Eco !$ + Hin d$$$ ,00 bp& %00 bp& (00 bp Eco !$ + Bam )$ *0 bp& ,*0 bp& %00 bp& *00 bp Hin d$$$ + Bam )$ *0 bp& ,00 bp& %*0 bp& *00 bp
Eco !$ + Hind $$$
Eco !$
Hind $$$
)
%00bp ,00bp (00bp
EcoRI + BamHI
EcoRI
BamHI
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*0bp ,*0bp %00bp *00bp
Hind $$$ + Bam )$
Hind $$$
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8/19/2019 General Genetics Assignment 3 Question 3
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)
Bam )$
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*0bp %*0bp ,00bp *00bp
-y comparing all the restriction fragment sizes& we can construct a restriction map
from the data. he restriction map is shown as below.
/et -1 be Bam )$.
/et 1 be Eco !$.
/et )1 be Hind $$$.
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0 *0 %00 300 200 900 bp4