Gas Chromatography. Gas Chromatography Basics Gas Liquid Chromatography (GLC) Gas Solid...

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Transcript of Gas Chromatography. Gas Chromatography Basics Gas Liquid Chromatography (GLC) Gas Solid...

  • Slide 1
  • Gas Chromatography
  • Slide 2
  • Gas Chromatography Basics Gas Liquid Chromatography (GLC) Gas Solid Chromatography (GSC) Mobile phase does not interact with analyte Separation occurs by interaction of analyte differentially w/liquid stationary phase and temperature GC preferred method, only applicable to volatile substances derivitiazation
  • Slide 3
  • Carrier Gas Supply Must be at a constant flow rate so that retention times & retention volumes may be equated Choice of detector will often Dictate the gases that are used, need to be inert Ex. FID used H2, air, and He
  • Slide 4
  • Injection Port Heated so sample virtually instantly turns into a vapor as sample plug goes onto column Septum Split/ SplitlessSplitSplitless Want to reproducibly get in & get out Sample focusSample focus
  • Slide 5
  • Flow rate Measurement Why is a known flow rate critical?
  • Slide 6
  • GC Columns Capillary: - have i.d.