Gas Chromatography (GC). Instruments for gas-liquid chromatography

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Transcript of Gas Chromatography (GC). Instruments for gas-liquid chromatography

  • Gas Chromatography

    (GC)

  • Instruments for gas-liquid chromatography

  • 1- Carrier gas supply2- Sample injection system3- Column configurationand column ovens4- Detection systems

  • 2- Sample injection system

  • 3- Column configurations and ovens3-1 Open tubular columns3-2 Packed columns3-3 Temperature programming

  • 3-1 Open tubular columnsWall-coated open tubular (WCOT)Support-coated open tubular (SCOT)Fused-silica open tubular (FSOT)

  • 3-2 Packed columnsSolid support materialsParticle size of supports

  • 3-3 Temperature programming

  • 4- Detection systemsFlame Ionization detectors (FID)Thermal conductivity detectors (TCD)Electron-capture detectors (ECD)Flame photometric detector (FPD)

  • Flame Ionization detectors (FID)

  • Thermal conductivity detectors (TCD)

  • Electron-capture detectors (ECD)

  • Flame photometric detector (FPD)

  • The stationary phasePolydimethyl siloxane [OV-1,SE-30]Poly(phenylmethyldimethyl)siloxane (10% phenyl) [OV-3,SE-52]Poly(trifluoropropyldimethyl)siloxan [OV-210]Poly(phenylmethyl)siloxane(50% phenyl) [OV-17]Polyethylene glycol [Carbowax 20M]Poly(dicyanoallyldimethyl)siloxane

  • Typical chromatograms with different stationary phases

  • Application of GCQualitative analysisQuantitative analysis

  • Stir-Bar Sorptive Extractionof Trace Organic CompoundsStir-bar sorptive extraction is a new solventless sample preparation method for the extraction and enrichment of organic compounds from aqueous matrices.

  • Stir bar sorptive

  • SBSE-GC-ECD/FPD in the Analysis of Pesticide Residues in Passiflora alata Dryander Herbal TeasSBSE in combination with GC-ECD/FPD analysis is here applied to the determination of 11 pesticides (hexachlorobenzene,lindane,chlorothalonil,parathion methyl,parathion ethyl,fenitrothion,malathion,dieldrin,- and -endosulfan,and tetradifon) in herbal teas prepared with Passiflora alata Dryander spicked leaves.

  • Experimental procedureMaterials and reagentsSample preparationCapillary GC-ECD/FPD analysisThermal desorption-capillary GC/MS analysis

  • Results and discussionSBSE-GC-ECD/FPD analysis of spiked waterSBSE-GC-ECD/FPD analysis of spiked P. alata Herbal teasSBSE-GC-ECD/FPD analysis of P. alata Herbal teas prepared with spiked plant material

  • Fig 1. GC-ECD & GC-FPD profiles of the SBSE extract from a water sample spiked with 0.5 pg/l of OC pesticides & 1.5 pg/l of OP pesticides:(1)hexachlorobenzene;(2)lindane;(3)chlorothalonil;(4)parathion methyl;(5)fenitorthion;(6)malathion;(7)parathion ethyl;(8)-endosulfan;(9)dieldrin;(10)-endosulfan;(11)tetradifon.

  • Fig 2.GC-ECD & GC-FPD profiles of the SBSE extract from a P.alata herbal tea spiked with 0.5 pg/l of OC pesticides & 1.5 pg/l of OP pesticides.

  • Table 3.Octanol/Water Partitioning Coefficients(Ko/w),Water Solubility,Recoveries(R%),RSD% from Spiked Herbal Teas and from Herbal Teas Made from Spiked Plant Material for the OC and OP Pesticides Investigated.

  • Fig 3.GC-ECD & GC-FPD profiles of the SBSE extract from a P.alata herbal tea prepared with plant material spiked with 50 ng/g of OC pesticides & 150 ng/g of OP pesticides.

  • Table 5.Intermediate Precision Expressed as RSD(%),LOD,LOQ for Each OC & OP Pesticide Investigated.

  • ReferencesSKOOG,HOLLER,NIEMAN; PRINCIPLES OF INSTRUMENTAL ANALYSIS ;FIFTH EDITION,(701-718),1999J. Agric. Food Chem.,51,27-33,2003

  • Thanks for your attention