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84 BIBLIOGRAPHY Books Guiron, F. J. (2000), Methods of Research and Thesis Writing, Mandaluyong City: National Bookstore Otto, J. H. et al., (1970), Modern Biology, Golden Art Printing Corporation, Diliman Quezon, City Unpublished Books Israila, R. B. (2012). Hydrophonics System for the Growth Characteristics of Okra (Abelmoschus esculentus L.) Plant , A Baby Thesis Presented to theFaculty of the College of Teacher Education Laguna State Polytechnic University, Sta. Cruz, Campus Ching, G. O. (2009). In vitro Cultivation of Jatropha curcas (Tubang- bakod: A Biodiesel Plant), An Experimental Thesis for the Degree of Master of Arts in Science and Technology, Laguna State Polytechnic University, Sta. Cruz, Campus Internet Articles Agud, E. et al. (2010). In Vitro Regeneration and Multiplication of the Apical and Nodal Meristem Derived from Some Potato Cultivars, Retrieved April 30, 2013 from http://science-on-

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BIBLIOGRAPHY

Books

Guiron, F. J. (2000), Methods of Research and Thesis Writing, Mandaluyong City: National Bookstore

Otto, J. H. et al., (1970), Modern Biology, Golden Art Printing Corporation, Diliman Quezon, City

Unpublished Books

Israila, R. B. (2012). Hydrophonics System for the Growth Characteristics of Okra (Abelmoschus esculentus L.) Plant, A Baby Thesis Presented to theFaculty of the College of Teacher Education Laguna State Polytechnic University, Sta. Cruz, Campus

Ching, G. O. (2009). In vitro Cultivation of Jatropha curcas (Tubang-bakod: A Biodiesel Plant), An Experimental Thesis for the Degree of Master of Arts in Science and Technology, Laguna State Polytechnic University, Sta. Cruz, Campus

Internet Articles

Agud, E. et al. (2010). In Vitro Regeneration and Multiplication of the Apical and Nodal Meristem Derived from Some Potato Cultivars, Retrieved April 30, 2013 from http://science-on-stage.web.cern.ch/Bulletin/UASVM/AnimalScienceandBiotechnologies, 67(1-2)/2010

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Al-amin, M. D. (2009). In vitro Micropropagation of Banana (Musa spp.),

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Anand, S. P. (2004). In vitro Multiple Shoot Regeneration from NodalExplants of Zehneria scabra (L.f.) Sonder – An Important Medicinal Climber Retrieved December 3, 2012 from http://docserver.esa.catchword.org/deliver/cw/pdf/esa/freepdfs/00222585/v42n4s19.pdf

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Bailey, R. V. et al. (2001). A Critical Test of the Two Prevailing Theories of Plant Response to Nutrient Availability, Retrieved April 5, 2013 from http://www.amjbot.org/content/90/1/143.full

Barth, F. et al.(2001).Factors Controlling High Efficiency of Adventitious Bud Formation and Plant Regeneration from In vitro Leaf Explants of Roses (Rosa hybrid, Retrieved March 8, 2013 from http://www.growingthehomegarden.com/2009/04/forest-pansy-redbud-cercis-canadensis.html

Bhosale, U. P. et al. (2011). In vitro Shoot Multiplication in Different Species

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Henry, D. (2010). Effect of BAP and IBA on In Vitro Regeneration, Shoot Multiplication and Rooting of Four Cultivars of Banana, Retrieved March 10, 2013 from http://www.springerlink.com/content/u48883n10578718v/

Hiremath, R. C. (2006). Micropropagation of Ginger (Zingiber officinale Rosc.), Retrieved November 12, 2012 fromhttp://www.exp-systems.com/2012/11/micro-prorogation-tissue-culture.pdf

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Lyam, P. T. et al., (2012). The Science of Plant Tissue Culture as a Catalyst for Agricultural and Industrial Development in an Emerging Economy, Retrieved November 14, 2012 fromhttp://www.exp-systems.com/2012/11/science-of-plant-tissue-culture.pdf

Malcom, G. F. (2013). In vitro Shoot Multiplication in Different Species of Banana, Retrieved August 12, 2012 from http://www.pelagiaresearchlibrary.com/Asian+Journal+of+Plant+Science+and+Research/2011/1/(3):23-27

Maloof, B. et al.(2001). Analyses of Essential and Edible oils and Constitu-ents therein, as Candidate Repellents for the Yellow Fever Mosquito Aedes aegypti l. (Diptera: culicidae), Retrieved September 7, 2012 fromhttp://www.hort.purdue.edu/newcrop/proceedings1990/V1-527.html

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Rabani, M. G. et al. (2011). Influence of Culturing Conditions on Growth and Sporulation of Drechslera hawaiiensis, the Foliar Blight Pathogen of Marsilea minuta L., Retrieved March 15, 2013 from http://thesissamples.blogspot.com/search/label/Chapter%202%20Review%20of%20Related%20Literature

Rai, R. (2007), Genetics and Plant Breeding: Introduction to Plant Biotechno-logy,Retrieved March 14, 2013 from http://www.google.com/revised+introduction+to+plant+biotechnology.pdf

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Sikder, M. et al. (2006). Macronutrient Content of Plant-based Food AffectsGrowth of a Carnivorous Arthropod, Retrieved May 27, 2013 fromhttp://theses.gla.ac.uk/5387/Journal of clinical microbiology 24 (15): 8732.pdfs

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APPENDIX A

PDA (Potato-dextrose-agar) MediumType of Medium

Explants in Vessel

Leaf Number

Average

Shoot Number

Averageper

Explantper

ExplantPDA-A Apical-A1 6

4.67

4

2.33PDA-A Apical-A2 4 2

PDA-A Apical-A3 4 1TOTAL 14 7

Table 15. Raw Data after Two (2) Weeks on PDA-Apical

Table 16. Raw Data after Two (2) Weeks on PDA- Nodal

Type of Medium

Explants in Vessel

Leaf Number

Average Shoot Number

Average

per Explant

per Explant

PDA-B Nodal-B1 1

6.33

2

2.00PDA-B Nodal-B2 14 2

PDA-B Nodal-B3 4 2

TOTAL 19 6

Table 17. Raw Data after Two (2) Weeks on CDA- Apical

CDA (Coconut-dextrose-agar) MediumType of Medium

Explants in Vessel

Leaf Number

Average Shoot Number

Average

per Explant

per Explant

CDA-A Apical-A1 5 5.00 1 1.00

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CDA-A Apical-A2 4 1

CDA-A Apical-A3 6 1TOTAL 15 3

Table 18. Raw Data after Two (2) Weeks on CDA- Nodal

Type of Medium

Explants in Vessel

Leaf Number

Average Shoot Number

Average

per Explant

per Explant

CDA-B Nodal-B1 4

4.00

2

1.67CDA-B Nodal-B2 4 1

CDA-B Nodal-B3 4 2TOTAL 12 5

Table 19. Raw Data after Two (2) Weeks on SDA- Apical

SDA (Saboraud-dextrose-agar) MediumType of Medium

Explants in Vessel

Leaf Number

Average Shoot Number

Average

per Explant

per Explant

SDA-A Apical-A1 6

5.33

1

1.67SDA-A Apical-A2 6 1

SDA-A Apical-A3 4 3TOTAL 16 5

Table 20. Raw Data after Two (2) Weeks on SDA- Nodal

Type of Medium

Explants in Vessel

Leaf Number Average Shoot Number Average

per Explant per ExplantSDA-B Nodal-B1 2

2.671

1.33

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SDA-B Nodal-B2 4 2

SDA-B Nodal-B3 2 1TOTAL 8 4

Table 21. Summary on Number of Leaves-Apical

Table 22. Summary on Number of Leaves-Nodal

SUMMARY

Groups Count Sum Average VariancePDA 3 19 6.33333 46.33333CDA 3 12 4 0SDA 3 8 2.66667 1.333333

Table 23. Summary on Number of Shoots-Apical

SUMMARYGroups Count Sum Average Variance

PDA 3 7 2.333333 2.333333CDA 3 3 1 0SDA 3 5 1.666667 1.333333

Table 24. Summary on Number of Shoots-Nodal

SUMMARY

Groups Count Sum Average Variance

PDA 3 6 2 0

CDA 3 5 1.666667 0.333333

SDA 3 5 1.666667 0.333333

SUMMARY

Groups Count Sum Average Variance

PDA 3 14 4.66667 1.333333

CDA 3 15 5 1

SDA 3 16 5.33333 1.333333

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B1 B2 B3

C1 C2 C3

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APPENDIX B

Figure 42. Proliferation of Leaves in PDA Apical Explants. (A1) PDA-A1; (A2) PDA-A2; (A3) PDA-A3

Figure 43. Proliferation of Leaves in CDA Apical Explants. (B1) CDA-A1; (B2) CDA-A2; (B3) CDA-A3

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E1 E2 E3

F1 F2 F3

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Figure 44. Proliferation of Leaves in SDA Apical Explants. (C1) SDA-A1; (C2) SDA-A2; (C3) SDA-A3

Figure 45. Proliferation of Shoots in PDA Nodal Explants. (D1) PDA-N1; (D2) PDA-N2; (D3) PDA-N3

Figure 46. Proliferation of Shoots in CDA Nodal Explants. (E1) CDA-N1; (E2) CDA-N2; (E3) CDA-N3

Figure 47. Proliferation of Shoots in SDA Nodal Explants. (F1) SDA-N1; (F2) SDA-N2; (F3) SDA-N3

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Figure 48. Well-grown Catnip (Nepeta cataria) herbs in two (2) weeks after transplantation. (A) PDA-Apical and Nodal; (B) CDA- Apical and Nodal; (C) SDA- Apical and Nodal