Evan H. DeLuciaFebruary 2018GI-CoRE

Approximate Geographic Distribution of Potential Dedicated Biomass Crops

Paterson et al. (2010) Annu. Rev. Plant Biol. 61: 349

chromosomes number: sorghum 10, Miscanthus 19, Saccharums at least 40

FEEDSTOCK ICON HERE

Producing oil in the plant factory

Zale et al. 2016. Plant Biotech. J.

Producing oil in the plant factory

GCB BioenergyVolume 10, Issue 2, pages 92-107, 7 SEP 2017 DOI: 10.1111/gcbb.12478http://onlinelibrary.wiley.com/doi/10.1111/gcbb.12478/full#gcbb12478-fig-0001

BIOREFINERY FOR COMBINED PRODUCTION OF JET FUEL AND ETHANOL FROM LIPID-PRODUCING SUGARCANE: A TECHNO-ECONOMIC EVALUATION

Biorefinery for combined production of jet fuel and ethanol from lipid-producing sugarcane: a techno-economic evaluation

GCB BioenergyVolume 10, Issue 2, pages 92-107, 7 SEP 2017 DOI: 10.1111/gcbb.12478http://onlinelibrary.wiley.com/doi/10.1111/gcbb.12478/full#gcbb12478-fig-0010

Kromdijk et al. Science 2016;354:857-861

https://www.agry.purdue.edu/ext/corn/news/timeless/yieldtrends.html

Illinois Biological Foundry for Advanced Biomanufacturing (iBioFAB)

iBioFAB: A scalable and automated platform for metabolic engineering

Ethanol fermentation with 1.1% (v/v) acetic acid by the wild type and a mutant yeast strain isolated using iBioFAB.

Si et al. Nature Communications DOI: 10.1038/ncomms15187

• fully automated workflow for yeast strain engineering

• CRISPR-assisted δ-integration to enable automation

• diverse genome-scale mutations (over-expression and down-regulation)

• a yeast strain with the highest reported acetic acid resistance was generated within a month, which harbors 68 kb mutations targeting 26 different genetic targets

• Platform can be used to rapidly engineer model and non-model yeasts for industrial applications

3.8 ha

0.7 ha

Flux measurements

NEP = GPP - ReNPP + Ra

Ra + Rh

Ecosystem Carbon Flux 2008 - 2013

-600

-400

-200

0

200

400

600

2008 2009 2010 2011 2012 2013

NE

CB

[g

C m

-2]

Year

Net ecosystem carbon balance (NECB)

Maize/Soybean

Miscanthus

Switchgrass

Prairie

Hudiburg et al. 2016. Nature Energy

Hudiburg et al. 2016. Nature Energy

Hudiburg et al. 2016. Nature Energy

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