ABSTRAK

TRI YULIANTI. Deteksi Keberadaan Antibodi Anti Diare (Escherichia coli dan Salmonella Enteritidis) dan Anti Flu Burung (H5N1) pada Kuning Telur Ayam Isa Brown yang Diberi Perlakuan Pemanasan Bertingkat. Dibimbing oleh AGUSTIN INDRAWATI dan RETNO DAMAYANTI SOEJOEDONO.

Penelitian ini bertujuan untuk mengetahui keberadaan antibodi (imunoglobulin Y) spesifik Escherichia coli, Salmonella Enteritidis, dan flu burung (H5N1) pada telur setelah proses pemanasan/perebusan. Pemanasan dilakukan dengan merebus telur selama 5 menit pada suhu 60C, 70C, 80C, 90C, dan 100C. Telur yang diujikan merupakan telur koleksi positif mengandung antibodi anti diare dan anti flu burung. Digunakan metode Agar Gel Precipitation Test (AGPT) untuk mendeteksi antibodi anti diare dan uji Hemaglutinasi Inhibisi (HI) untuk mendeteksi antibodi terhadap flu burung. Berdasarkan penelitian ini didapatkan hasil bahwa pada telur yang dipanaskan suhu 60C, 70C, dan 80C selama 5 menit keberadaan antibodi (IgY) masih dapat terdeteksi dengan menggunakan metode AGPT dan HI. Antibodi spesifik untuk Escherichia coli, Salmonella Enteritidis, dan flu burung (H5N1) bertahanan pada pemanasan telur dikisaran suhu 6080C. Sedangkan pemanasan telur selama 5 menit pada suhu 90C dan 100C sudah tidak terdeteksi adanya antibodi spesifik karena antibodi (IgY) yang terdiri dari protein sudah terdenaturasi. Dapat disimpulkan bahwa semakin tinggi suhu pemanasan telur aktivitas biologi IgY semakin menurun.

ABSTRACT

TRI YULIANTI. Detection of existance Anti-diarrheals (Escherichia coli and Salmonella Enteritidis) and Anti-Avian Influenza (H5N1) Antibody at Isa Brown Egg Yolk Chicken with warming various level treatment. Supervised by AGUSTIN INDRAWATI and RETNO DAMAYANTI SOEJOEDONO.

This study was aimed to detect the existence of antibodies (immunoglobulin Y) specific to Escherichia coli, Salmonella Enteritidis, and Avian influenza (H5N1) in eggs after heating/boiling process. The treatment is by heating or boiling the whole eggs complete with shells at a temperature of 60C, 70C, 80C, 90C and 100C for 5 minutes. The Eggs were tested that containing antibodies of anti-diarrhea and anti-avian influenza. This research used Agar Gel Precipitation Test (AGPT) method to detect antibodies of anti-diarrhea and hemagglutination inhibition (HI) test to detect antibodies of avian influenza. The results showed that the presence of antibodies (IgY) can still be detected by using AGPT and HI method in the eggs which be heated at 60C, 70C and 80C for 5 minutes. Antibodies specific for Escherichia coli, Salmonella Enteritidis, and avian influenza (H5N1) could survive in the eggs complete with eggshells which be heated in the range of temperature 6080C. While on heating 90C and 100C has not detected the presence of antibodies specific because antibodies which is consist of protein was denatured. It could be concluded that the higher of egg heating temperature, IgY biological activity were decrease.