CRYOSTAT - Tanner Scientific · in this user manual. Tanner Scientific ... This cryostat may only...

User Manual

TN9000CRYOSTATSemi-Automatic Cryostat

Read Prior to Operation!Always Keep This Manual Near the Unit!

57515 07.21.16

InformationThe information, notes, and figures that appear in this user manual represent the present state of knowledge as comprehended. Due to continuous improvements in technology and manufacturing techniques, specific and manufacturing procedures may change.

For inaccurate statements, drawings, and technical illustrations in this user manual, we exclude liability as far as permissible. No liability is accepted for any monetary loss or consequential damage caused by or related to compliance with statements or other information in this user manual. Tanner Scientific® will update the manual as needed according to the latest technological developments.

This document is protected under copyright laws. Any reproduction of illustrations or text requires prompt documented consent by Tanner Scientific®.

Statements, drawings, illustrations and other information regarding content or technical details of the present user manual are not to be considered as warranted characteristics of our products. These are determined only by the contract provisions agreed between us and our customers.

Refer to the name plate on the back of the unit for the serial number and manufacturing date of this instrument.

Manufactured byTanner Scientific1107 Tallevast RdSarasota, Florida 34243

[email protected]

Tanner Scientific® TN9000 • Semi-Automatic Cryostat 1

1 | General Summary...................................................................................................4

2 | Safety Precautions................................................................................................5

3 | Technical Parameters.........................................................................................6

4 | Installation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7

5 | Operation...........................................................................................................8

6 | Cleaning & Maintenance..............................................................................23

7 | Warranty & Service............................................................................................25

8 | Troubleshooting...........................................................................................26

Contents

5.1 | Blade Holder.....................................................................................................................8

5.2 | Specimen Disc Installation.................................................................................11

5.3 | Freezing Specimen...................................................................................................12

5.4 | Defrost . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13

5.5 | Vertical View.................................................................................................................14

5.6 | Touch Screen Display............................................................................................14

5.7 | Auxiliary Function Setting.................................................................................17

5.8 | Time Setting.................................................................................................................18

5.9 | Course Feed Control Panel...............................................................................20

5.10 | Maintenance...........................................................................................................22

4 Tanner Scientific® TN9000 • Semi-Automatic Cryostat

1 | General Summary

The TN9000 Semi-Automatic Cryostat Microtome is a medical device used for rapid freezing of pathological sections of human and animal tissue. It can be widely used for pathological diagnosis, analysis and research in hospitals, medical colleges, and by legal medical experts and institutes.

Instrument Consists of 4 Major Parts:• The touch screen display is located on the upper part of the instrument.

This area displays the general working conditions and the temperature of the instrument.

• The chamber area is used to rapidly freeze the tissue and to conduct cutting. The microtome assembly is located inside the chamber area.

• The compressor, cooling condenser and evaporator are located in the lower part of the instrument.

• Behind the chamber is the mechanical transmission and motor drive.

Hand Wheel

Power Switch

Touch Screen Display

Control Panel

Glass Window

Stabilizing Feet

Liquid Waste Bottle


2 | Safety Precautions

This user manual includes important instructions and data related to the operating safety and maintenance of the instrument. The user manual is an important part of the product. It should be read carefully before using the instrument for the first time and must always be kept with the instrument.This cryostat may only be operated by qualified personnel. In case of malfunction, contact a qualified service technician. Never try and solvethe problem yourself.

Power Supply• A professional electrical engineer should inspect the electrical circuit before

installation to ensure smooth operation of the instrument.

• The instrument uses a single phase AC power supply: the voltage is110V±10%. If the power does not meet this requirement, then a2000-3000VA AC voltage regulator shall be connected for usage.

• A dedicated 20AMP circuit is required.

• Confirm the power supply is well grounded (triple socket) before connecting the instrument with the main power socket. Turn on the power switch in the back of the instrument to start the machine.

Temperature • The cryostat’s working (environment) temperature is 5°C ~ 23°C (40°F ~ 73°F).

Operating• Exercise extreme caution when handling blades. Always remove when

cleaning the inside of the chamber. Never place a blade face up as the sharp surface can lead to serious injury.

• NEVER TRY TO CATCH A FALLING BLADE!

• Before changing specimens, always lock the hand wheel and cover the blade edge with the blade guard. Make sure the hand wheel is in the locked position when the instrument is not in use.

High temperature or humidity will impact the chamber temperature and shorten compressor life.


3 | Technical Parameters

Section Thickness Range: 1-90µm Adjustable, 1-20µm, 1µm increments 20-40µm, 2µm increments 40-90µm, 5µm increments

Trimming Thickness Range: 10-400µm Adjustable, 10-50µm, 5µm increments 50-100µm, 10µm increments 100-400µm, 50µm increments

Specimen Retraction: to 100µm Adjustable

Horizontal Stroke: 1”

Vertical Stroke: 1.75”

Specimen Orientation: 8°C (X, Y, X Three Directions)

Voltage: 110V±10%, 20AMP

Frequency: 50Hz

Power: 850VA

Largest Start-Up Current: (5 sec) 45A

Chamber Temperature: -10°C ~ -35°C Adjustable

Freezing Shelf Temperature: -45°C

Number of Freezing Stations: 18

Peltier Number: 2

Refrigerant: R404a, 300g±10g

Compressor Oil: 0.6L EMKARATE RL-22S, ICI

DimensionsWidth (w/o Hand Wheel): 25.5”

Width (w/Hand Wheel): 30.5”

Depth: 27.6”

Height: 50”

Weight: 390lbs


4 | Installation

Transportation/Unpacking• Unit must be shipped in the upright position. If the unit was shipped or stored

lying down, it must stand upright for 36 hours before use so all lubricating oils enter the compressor. It should only be used after 4 hours of standing upright to ensure proper lubricant distribution.

• Inspect the package for damage. If damaged, do not open and immediately contact Tanner Scientific® and the transportation carrier.

Site Requirements• Do not operate in rooms with or near flammable chemicals.

• Place instrument at least 10” from other instruments. Areas on both sides of the instrument should be clear to allow proper air flow to the cooling unit.

• Do not place in rooms above 75°F or in a humid environment directly in sunshine, OR NEAR AC VENTS OR CEILING FANS. The environment’s relative humidity should be no more than 80%. Failure to follow this procedure could create frost and icing in the chamber.

Placement • The cryostat is a movable floor model with 4 caster wheels and 2

stabilizing legs in the front that are adjustable to level the instrument.

Hand Wheel Assembly• Insert the pin of the hand wheel shaft into the hole of the hand wheel.

• Mount the spring washer on the screw (M10x15).

• Tighten the screw with a #6 Allen key.

Freezing Chamber Assembly • Place the waste tray in the freezing chamber.

• Install blade holder on the microtome base and lock.

• Place all tools needed for specimen preparation in the chamber.

• Close glass window.

• Connect power.

Check your power socket to ensure it is well grounded and that the power supply is stable, constant and accurate.


5.1 | Blade Holder

5.1.1 | Blade Holder Adjustment

Turn the blade holder lock handle clockwise to release it. Manually move back and forth and lock in the desired position. When the holder moves to the terminal point, the entire blade holder can be taken out and put back by reversing the procedure. Turn (counter clockwise) the blade holder lock handle. Manually move left and right and lock in the desired position.

Notice: Remember to lock the blade holder in the correct position after moving it forwards or backwards.

Lock Anti-Roll Plate Knobs

Clamping LeverFor Locking

Clamping LeverLocks the Blade Holder(Moving Left and Right)

Clamping Leverfor Adjusting Angle

Clamping Leverfor Anti-Roll Plate

Knob Adjusts Heightof Anti-Roll Plate

Clamping LeverLocks the Blade Holder

(Moving Back and Forth)

5 | Operation


Tissue

Side Face of Blade

Anti-Roll Plate

Adjustable Angle

Angle of Blade Edge

5.1.2 | Changing the Blade

5.1.3 | Adjusting the Angle of the Blade

Release the clamping lever on the upper right to open the blade holder.Insert blade from left to right and then lock it in place.

Loosen the lowest adjustment handle on the right side of the blade holder. Refer to the reference scale to turn the rotator in order to choose the best cutting angle of the blade. The angle should be no more than 10°. Retighten the adjustable handle.

Note: Blade is extremely sharp. Use caution when handling or replacing blades.

30°

10°


5.1.4 | Adjust Anti-Roll Plate Angle with Blade

1) Make the edge of the anti-roll plate parallel to the edge of the blade.• Insert blade and lock the clamping lever.

• Lock two knobs.

• Use hexagonal wrench to make blade and anti-roll plate parallel.

• Fasten hexagonal screw.

• Turn knob to adjust height of the anti-roll plate.

2) Make the edge of anti-roll plate and the edge of the blade flush.• Turn height turning knob to adjust height of the anti-roll plate.

• Adjust accordingly until you get quality sections.

1) The upper part of the anti-roll plate should not touch the specimen.

2) When the quality of the tissue section is lacking, check the edge of the blade for foreign matter.

3) Avoid touching the front of the plate.

Edge of Anti-Roll Plate and Blade Flush

Anti-Roll Plate Height Turning Knob

Adjust with Hex Wrench.Make the Edge of Anti-Roll Plate and Edge of Blade Flush.

Edge of Anti-Roll Plate

Edge of Blade


5.2 | Specimen Disc Installation

• Adjust the specimen clamp accordingly.

• After the specimen has been frozen on the specimen disc, insert specimen disc shaft into the hole in the center of the clamp. Join the front surface ofthe clamp with the rear surface of the specimen disc. Rotate the screw to lock the front of the clamp.

• Loosen adjustable clamp screw, turn the lever to adjust the direction of the specimen disc surface and lock.

Specimen DiscLock Screw

Adjust & LockClamp Screw

Specimen Disc

Clamp Adjust Lever


5.3 | Freezing Specimen

The specimen freezing station is located at the left side of the chamber. There are 18 holes to place the specimen disc, 2 of which are semiconductor refrigeration holes. The temperature of the freezing station is always colder than the freezing chamber’s temperature.

• At room temperature, cut the specimen to an appropriate size. Place on disc and fix with tissue freezing medium.

• Insert specimen disc with specimen into the freezing hole.

• Hold the freezing clamp on the specimen for about 30 seconds or until specimen is completely frozen.

• After specimen is frozen, insert specimen disc into the specimen clamp, lock, and begin sectioning process.

1) Movable Support

2) Heat Extractor

3) Specimen Hole

4) Specimen Disc


5.4 | Defrost

In some situations, it makes sense to allow the machine to defrost and eliminate excess ice buildup. When the defrost cycle runs, ice will melt and drain into the waste container.

• The defrost cycle runs for almost 15 minutes.

• Upon completion of the defrost cycle, the machine will automatically return to its original cold state.

Automatic Defrost TimerSet the automatic defrosting time accordingly. When the chamber’s temperature reaches -5°C, defrost automatically terminates.

Instant Manual DefrostSet the manual defrost at any time. It can be turned off if needed.


5.5 | Vertical View

5.6 | Touch Screen Display (Work Area)

Touch ScreenDisplay

Hand Wheel

Power Button

Lighting& UV LampTouch Pad


5.6.1 | Temperature Setting

Delay Startup ModeWhen the compressor’s downtime is less than three minutes, and is started again, the compressor will enter delay startup mode. The compressor will become fully operational after 3 minutes.

1) Actual Temperature (Reads the Chamber’s Current Temperature)

2) Set Temperature (Use +/- to Increase or Decrease Temperature)

3) Compressor “On” (Indicates if the Compressor is Running or Not)

5.6.2 | Section & Trimming Section

1) Current Section Thickness (5µm) (Use +/- to Increase or Decrease)

2) Current Trimming Thickness (10µm) (Use +/- to Increase or Decrease)

3) Switch Key (Toggles Between Section and Trimming)

4) Thickness Setting Key (Adjusts Thickness)

5) Total Section Thickness (Calculates Total Amount of Section Cut)


5.6.3 | Start Freezing Stage

5.6.4 | Lighting and UV Lamp

Freezing will stop when countdown reaches 0.Press Freezing Time button at any time to stop freezing.

Working Conditions1) Freezing Stage Temperature < -10°C

2) Working Compressor On

If these two conditions are not satisfied, electronic cooling automatically stops. Countdown will automatically restart when conditions are met.

Note: UV disinfection will only activate when the glass door is closed, which means the UV lamp can not work when the glass door is open. When the lamp is on, opening the door causes the light to automatically turn off. Close the glass door and press the UV button, the lamp will light up again.

LIGHT ON

LIGHT OFF

UV ON

UV OFF

1) Temperature of Freezing Stage (Specimen Chuck Freezing Stage)

2) Freezing Timer (Reads How Much Time is Left in the Freezing Cycle)

3) Freezing Time (900 Seconds: 900S)


Set the Time of Day the Cryostat Enters Defrost Mode and Sleep Mode.

1) Manual Defrost

2) Automatic Defrost Settings from Sunday to Saturday.Press square below each day to activate. Selected Unselected

3) Timer. Press the black square to display timer.Choose hour and minutes.

Press to increase or decrease time.

Click SAVE or CANCEL to save or cancel the time setting. Each defrost time takes 10 minutes.

5.7 | Auxiliary Function Setting

5.7.1 | Defrost Setting


1) Manual Sleeping Mode. Chamber should keep running at -5°C while the display light dims. To deactivate sleep mode, press the button at any time.

2) Automatic Sleeping Mode. Without any activity after two hours, the machine automatically enters sleep mode.

3) Automatic Sleeping Mode Start Time and End Time.

Note: After setting up the time, you need to press the SAVE button.Otherwise, the setting will not enter sleep mode.

5.7.2 | Sleeping Mode Setting

5.8 | Time Setting


5.8.1 | System Time Setting

5.8.2 | Setting Retraction Value of Section

Select (+/-) to increase or decrease the retraction value. The range of retraction value is 0-100µm with 5µm increments. REMEMBER TO SAVE.

1) Select the box under the workday you want to set a time. refers to the corresponding workday you selected.

2) Select the black square to display time. Choose hour and minutes.

Select to increase or decrease time.

3: Press SAVE to save the setting. At the same time, the gray bar in the lower right corner of the screen will display your set time.


5.9 | Course Feed Control Panel

Located to the left of the main chamber, the course feed control panel allows you to advance or retract the specimen. Additionally, you can control the thickness by using the +/- buttons.

You can increase the speed at which the specimen advances or retracts by holding down the “F” button at the same time pressing the or buttons.

TIP: Before adjusting any specimen, make sure the hand wheel is locked.

5.9.1 | Trimming

Trimming can be controlled on the display screen and the touch pad located to the left of the chamber. Use the +/- buttons to increase or decrease the trimming thickness with each turn of the hand wheel.

Trimming allows you to cut away unwanted portions of the specimen.

• Place the blade into the holder and lock. Adjust the anti-roll plate.

• Adjust the blade holder’s angle 1°~2°, or to the angle that works best.

• Adjust holder and sample, but keep enough space for depth of cutting.

• Open the anti-roll plate.

• Unlock the hand wheel.

• Move the sample close to blade. Turn the handwheel to section.Thickness can be set to 20µm. Reduce gradually to the desired thickness.

• Close the anti-roll plate.

• Turn hand wheel to trim excess specimen away.


5.9.2 | Sectioning Mode

5.9.3 | Sectioning Considerations

• Press the “Switch” button to enter sectioning mode.

• Turn the hand wheel one rotation. The instrument will cut one piece of section at a time.

• It’s best to turn the hand wheel at uniform speed.

• Adjust speed until you receive quality sections.

1) When using the cryostat, controlling the blade’s speed and correctly adjusting the anti-roll plate are important factors in cutting high quality sections. The proper speed of the blade can only be acquired by practice and experience, along with skillful control of the hand wheel. The proper adjustment of the anti-roll plate requires even more practice. Sometimes they interfere with each other and require careful adjustment.

2) When freezing tissue, the water within the tissue will condense into ice and the tissue will harden. The hardness changes with the temperature: the lower temperature, the harder the tissue becomes. Each tissue reacts differently under various temperatures. Achieving high quality sections can only be obtained through practice. Cutting temperatures of the specimen tissue without fat and formalin should be between -13°C ~ -25°C.

3) For high quality sectioning, pay attention to the following: • Proper working temperature in the chamber • Correct hand wheel speed • Fine adjustment of the anti-roll plate • Sharp blade edge and correct blade angle 4) Frozen sections should not be cut in wide sections. Cutting thinner sections, so that the line of contact is shorter, is ideal.


5.10 | Maintenance

5.10.1 | Daily Finishing Work• Lock the hand wheel.

• Remove the blade from the holder accordingly.

• Remove waste from the chamber.

• Clean blade holder and brush holder.Tip: General cleaning can be used with 95% ~ 98% alcohol.

• Close the glass window.

• Turn off the lights.

• Do not turn off the main switch. Do not stop the cooling system when the compressor is running.

5.10.2 | Power Up/Down

If you go several weeks or longer without using a machine, you can turn off the main power switch.

• Turn off the machine’s power switch when compressor is not running.

• Open the glass window, ventilate the freezing chamber and let dry.

• Remove all specimens from the freezing shelf.

• Lock the hand wheel.

• Remove the blade from the blade holder.

• Remove waste.

• Remove the dust pan and brush to clean.

Before restarting the machine, make sure all parts in the freezing chamber are thoroughly dry or icing could occur.


6 | Cleaning & Maintenance

6.1 | Daily Cleaning• Dispose of any waste daily.

• Clean the waste pan.

• Clean the brush holder.

• Waste water treatment must comply with local environmental regulations.

• Observe the water level in the bucket and discard accordingly.

6.2 | Maintenance• Clean the machine daily.

• Add droplets of low temperature lubricating oil on the plastic coupling interface every week.

• Lubricate the Specimen Feed Mechanism Weekly: Click the fast forward button to make the clamp move to the front position, drip a drop of the low temperature lubricating oil on the copper bushing, then click the fast rewind button to make the clamp move to the back position.

• Lubricate the Eyelet Cover As Needed: Turning the hand wheel, drip some drops of the low temperature lubricating oil on the decorative sheeting when the specimen clamp on the highest and lowest position, then turn the hand wheel again to make the oil distribute evenly.

• Clean the Condenser As Needed: Open the vent panel at right of the machine, clean the dust on the cooling fin of the condenser with brush,or jet it with high-pressure airflow.

Since these machines are precision instruments, it’s important to pay attention to cleanliness, maintenance and repair work. Do NOT use organic solvents or any aggressive substances to clean and sterilize the chamber! Only use the cleaning agents specified in this manual. If necessary, the external surface may be cleaned with light-duty commercial household cleaner. Use a wet cloth and rub until dry. Do NOT apply xylene or glass cleaner.


6.4 | Replacing Fuses

• Turn off the machine and unplug the power cord.

• Replace the Fuse: There are 5 fuse boxes in the back of the instrument. Open the cap and remove the broken fuse tube. Insert a new fuse into the lid. Close the lid on the fuse box and tighten.Note: You can only use the same type of fuse!

6.5 | Waste Bottle

The waste bottle is used to collect the liquid from defrosting tissue, washing fluid and waste.

Pour 200mL of 10% formalin solution or other disinfectant into the water bucket in order to prevent waste damage on personnel and environment. Waste discharge should be in accordance with local regulations. Use correct methods to treat the waste water and waste.

1) Light 1.5A

2) Defrost 3A

3) Anti-Fog 3A

4) Compressor Overcurrent ProtectorThe button bounces when there is too much electric current. Press button to restore.

5) UV Lamp 1.5A

6) Thermoelectric Cooler 5A

Note: The standard fuse is 6*30mm.

1

4

2

5

3

6


7 | Warranty & Service

Tanner Scientific® warrantees this product for a period of one year from the date of purchase, provided that the customer complies with the rules of this user manual. Extended warranties are available. Contact Tanner Scientific® at 888.708.5233 for more information. Tanner Scientific® is not responsible for any damage or other issues caused by abuse and misuse of the instrument.

Service Information If you require any service or parts during the warranty period, please contact Tanner Scientific® at 888.708.5233, or the sales agent who sold you the unit. Prior to calling, please have the instrument model and serial number ready.

AmendmentTanner Scientific® reserves the right to change the technical parametersof any model for improving the function of the instruments.

Quality Guarantee • Tanner Scientific® will ensure that every instrument sold has been strictly

examined to ensure it meets the stringent quality and technical standards.

• Service terms are only provided for those who regularly use the instrument and operate the instrument according to its instructions.

Disposal of Unwanted InstrumentsDiscarding the instrument and its parts should be done according to current laws and regulations.


8 | Troubleshooting

PROBLEMS C AUSE S ELIMINATION

• Section melts• Temperature of the specimen,

blade, anti-roll plate or blade holder is not low enough

• Set lower temperature

• Section splinters• Temperature of the specimen is

not low enough• Set higher temperature

• Section uneven

• Temperature of the specimen is not low enough

• Set lower temperature

• Specimen is too big• Trim the plane of the specimen

and increase the thickness• Relative position of the anti-roll

plate’s edge and the edge of the blade is not correct

• Adjust the anti-roll plate

• Cutting angle is not correct • Adjust the angle of the blade

• Blunt blade• Use other part of the blade to

cut or replace with new blade• Although the temperature and

the position of the anti-roll plate are correct, the sectionis uneven.

• Residue on the blade or theanti-roll plate

• Clean

• Section curls• The clearance between the edge

of the anti-roll plate and the cutting edge is too small

• Increase the clearance

• Odd sounds when slicing

• The clearance between the edgeof the anti-roll plate and the cutting edge is too small or the edge of the anti-roll plate has touched the specimen

• Adjust the anti-roll plate

• Damaged blade• Use other part of the blade to

cut or replace with new blade• The edge of the anti-roll plate

has been damaged• Use new anti-roll plate

• Tissue section is sticking or broken on the anti-roll plate

• The temperature of theanti-roll plate is too highor its position is not correct

• Reduce the temperature andadjust the position of theanti-roll plate

• Anti-roll plate is electrostatic • Remove electrostatic• Residue on the anti-roll plate • Clean• Rusty blade • Replace with new blade


PROBLEMS C AUSE S ELIMINATION

• Section elevates

• The freezing temperature of the specimen is not adequate

• Continue to freeze

• Specimen disc is not fastening • Review and lock in place• The knuckle of the specimen

clamp is not fastening• Review and lock in place

• The blade is not fastening • Review and lock in place• The specimen is loose from the

disc after slicing• Retighten the specimen

• Section is uneven and too hard• Increase the thickness and

reduce the area


cut or replace with a new blade• Wrong type of blade • Use correct type of blade• Wrong cutting angle • Adjust the angle of cutting

• Tremor when slicing

• Wrong temperature • Adjust the temperature• Wrong type of blade • Use correct type of blade• The rotation speed of the hand

wheel is wrong• Adjust the speed and rotation

• Loose blade • Review and lock in place• The specimen is loose from the

disc after slicing• Retighten the specimen


cut or replace with a new blade• Wrong cutting angle • Adjust the angle of cutting• The machine is not dry thoroughly

before turning power on• Make sure the machine is dry

• Specimen is too dry • Use new specimen

• Section curls on theanti-roll plate

• Influenced by the air or static • Remove static• Temperature of anti-roll

plate is too high• Reduce the temperature

• Broken tissue section

• Temperature section is too low • Increase the temperature


cut or replace with a new blade• Residue on the blade or the

anti-roll plate• Clean

• The edge of the anti-roll platehas been damaged

• Use new anti-roll plate

Manufactured byTanner Scientific1107 Tallevast Rd

Sarasota, Florida 34243

[email protected]

CRYOSTAT - Tanner Scientific · in this user manual. Tanner Scientific ... This cryostat may only...

Documents

Transcript of CRYOSTAT - Tanner Scientific · in this user manual. Tanner Scientific ... This cryostat may only...