Confocal Laser Microscopy at the MHH

Confocal Laser Microscopy at the MHH

Laser Microscopy Facility

Zentrale Forschungseinrichtung Lasermikroskopie

Location:

Building I4, Level 01

Contact:

Rudolf Bauerfeind

Wolfgang Posselt

konfokal@mh-hannover.de

Tel.: -3914

Confocal Laser Microscopy at the MHH

Please,

visit the web page!

http://www.mh-hannover.de/lasermicroscopy.html

Confocal Laser Microscopy at the MHH

How does a confocal microscope work?

focal plane

confocal pinhole

confocalwide field

Confocal Laser Microscopy at the MHH

What is the advantage of using a confocal laser scanning microscope?

• optical sectioning

• 3D-imageing

• x-z sectioning

• better resolution

• easy multi colour fluorescence

• advantages of laser scanning

• bleaching (FRAP, …)

• ROI scan (photoactivatable

fluorescent proteins)

Katinka Döhner, Virology, MHH

Confocal Laser Microscopy at the MHH

Which fluorescent dyes can I use? Available laser lines [nm]

405

442

458

476

488

496

514

543

594

633

MP 710-920

The choice is only limited by the excitation wave length

Confocal Laser Microscopy at the MHH

Live cell imaging

• Incubation chambers with control of

temperature, humidity, and CO2

for cells grown on glass

• In situ microscopy using water immersion

long distance objectivesChristian Hähnel, Cell Biology

Confocal Laser Microscopy at the MHH

Microscopes and applications

Zeiss META

Leica inverted-2

Leica inverted-

MP

Leica Upright-

MP

Leica Upright-2

Olympus FV1000

Image Xpress

fixed samples

yes yes yes low

resolution yes yes yes

live cell imaging

37°C, CO2 37°C, CO2 37°C, CO2 low

resolution no 37°C, CO2 no

UV no yes no no no yes -

multi-photon

no no yes yes yes no -

good for fast live

cell blue DNA

stains FLIM, FCS

in situ: organs – animals

orange-red dyes (561nm)

slow live cell (hours – days); FRAP

high through

put screening

• The laser microscopy facility is a S2 laboratory

• A cell culture with 2 safety hoods and 2 incubators

can be used to prepare your experiments.

• Computer with full versions of the microscope software

and 3d-imaging software are available

Confocal Laser Microscopy at the MHH

Additional facilities

Confocal Laser Microscopy at the MHH

How can I use the facility?1. Send an e-mail: konfokal@mh-hannover.

2. We will discuss your project.

3. A training session will instruct you

in microscope operation and laser safety.

Bring your own samples!

4. You can use the microscope on your own.

Reservation are made through WWW.

The facility is accessible 24 hr.

5. User will be charged with a user fee:

3-5 Euros per hr for MHH scientists.