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Transcript of Cell Structure and Function 1. Agre and cells in the news 2.
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Cell Structure and Function
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Agre and cellsin the news
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Cells
• Smallest living unit• Most are microscopic
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Discovery of Cells
• Robert Hooke (mid-1600s)– Observed sliver of cork– Saw “row of empty boxes”– Coined the term cell
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Cell theory
• (1839)Theodor Schwann & Matthias Schleiden
“ all living things are made of cells”
• (50 yrs. later) Rudolf Virchow
“all cells come from cells”
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Principles of Cell Theory
• All living things are made of cells
• Smallest living unit of structure and function of all organisms is the cell
• All cells arise from preexisting cells
(this principle discarded the idea of
spontaneous generation)6
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Cell Size
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Cells Have Large Surface Area-to-Volume Ratio
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Characteristics of All Cells
• A surrounding membrane
• Protoplasm – cell contents in thick fluid
• Organelles – structures for cell function
• Control center with DNA
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Cell Types
• Prokaryotic
• Eukaryotic
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Prokaryotic Cells
• First cell type on earth
• Cell type of Bacteria and Archaea
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Prokaryotic Cells
• No membrane bound nucleus
• Nucleoid = region of DNA concentration
• Organelles not bound by membranes
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Eukaryotic Cells• Nucleus bound by membrane
• Include fungi, protists, plant, and animal cells
• Possess many organelles
Protozoan 13
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Representative Animal Cell
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Representative Plant Cell
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Organelles
• Cellular machinery
• Two general kinds– Derived from membranes– Bacteria-like organelles
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Bacteria-Like Organelles
• Derived from symbiotic bacteria
• Ancient association
• Endosymbiotic theory– Evolution of modern cells from
cells & symbiotic bacteria
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Plasma Membrane
• Contains cell contents
• Double layer of phospholipids & proteins
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Phospholipids
• Polar– Hydrophylic head– Hydrophobic tail
• Interacts with water
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Movement Across the Plasma Membrane
• A few molecules move freely– Water, Carbon dioxide, Ammonia, Oxygen
• Carrier proteins transport some molecules– Proteins embedded in lipid bilayer– Fluid mosaic model – describes fluid nature of
a lipid bilayer with proteins
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Membrane Proteins
1. Channels or transporters– Move molecules in one direction
2. Receptors – Recognize certain chemicals
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Membrane Proteins
3. Glycoproteins – Identify cell type
4. Enzymes – Catalyze production of substances
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Cell Walls
• Found in plants, fungi, & many protists
• Surrounds plasma membrane
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Cell Wall Differences
• Plants – mostly cellulose
• Fungi – contain chitin
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Cytoplasm• Viscous fluid containing organelles• components of cytoplasm
– Interconnected filaments & fibers – Fluid = cytosol– Organelles (not nucleus)– storage substances
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Cytoskeleton• Filaments & fibers
• Made of 3 fiber types– Microfilaments– Microtubules– Intermediate filaments
• 3 functions:– mechanical support– anchor organelles– help move
substances
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Actin
• Found inside of filaments
• Actin is a globular multi-functional protein that forms microfilaments. It is found in all eukaryotic cells.
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A = actin, IF = intermediate filament, MT = microtubule
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Cilia & Flagella
• Provide motility• Cilia
– Short– Used to move substances
outside human cells
• Flagella – Whip-like extensions– Found on sperm cells
• Basal bodies like centrioles
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Cilia & Flagella Structure
• Bundles of microtubules
• With plasma membrane
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Centrioles
• Pairs of microtubular structures
• Play a role in cell division
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Membranous Organelles
• Functional components within cytoplasm
• Bound by membranes
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Nucleus
• Control center of cell
• Double membrane
• Contains – Chromosomes– Nucleolus
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Nuclear Envelope
• Separates nucleus from rest of cell
• Double membrane
• Has pores
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DNA
• Hereditary material
• Chromosomes– DNA– Protiens– Form for cell division
• Chromatin
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Nucleolus
• Most cells have 2 or more
• Directs synthesis of RNA
• Forms ribosomes
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Endoplasmic Reticulum
• Helps move substances within cells
• Network of interconnected membranes
• Two types– Rough endoplasmic reticulum– Smooth endoplasmic reticulum
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Rough Endoplasmic Reticulum• Ribosomes attached to surface
– Has NON-FREE RIBOSOMES– Manufacture protiens– Not all ribosomes attached to rough ER
• May modify proteins from ribosomes
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Smooth Endoplasmic Reticulum
• No attached ribosomes
• Has enzymes that help build molecules– Carbohydrates– Lipids
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Golgi Apparatus
• Involved in synthesis of plant cell wall
• Packaging & shipping station of cell
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Golgi Apparatus Function
1. Molecules come in vesicles
2. Vesicles fuse with Golgi membrane
3. Molecules may be modified by Golgi
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Golgi Apparatus Function (Continued)
4. Molecules pinched-off in separate vesicle
5. Vesicle leaves Golgi apparatus
6. Vesicles may combine with plasma membrane to secrete contents
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Lysosomes
• Contain digestive enzymes• Functions
– Aid in cell renewal– Break down old cell parts – Digests invaders
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Vacuoles
• Membrane bound storage sacs
• More common in plants than animals
• Contents – Water– Food– wastes
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Bacteria-Like Organelles
• Release & store energy
• Types – Mitochondria
(release energy) – Chloroplasts
(store energy)
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Mitochondria
• Have their own DNA
• Bound by double membrane
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Mitochondria
• Break down fuel molecules (cellular respiration)
– Glucose– Fatty acids
• Release energy– ATP
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Chloroplasts
• Derived form photosynthetic bacteria
• Solar energy capturing organelle
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Photosynthesis
• Takes place in the chloroplast
• Makes cellular food – glucose
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Review of Eukaryotic Cells
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Review of Eukaryotic Cells
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Molecule Movement & Cells
• Passive Transport
• Active Transport
• Endocytosis
(phagocytosis & pinocytosis)
• Exocytosis
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Passive Transport
• No energy required
• Move due to gradient– differences in concentration, pressure, charge
• Move to equalize gradient– High moves toward low
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Types of Passive Transport
1. Diffusion
2. Osmosis
3. Facilitated diffusion
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Diffusion
• Molecules move to equalize concentration
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Osmosis
• Special form of diffusion
• Fluid flows from lower solute concentration
• Often involves movement of water– Into cell– Out of cell
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Solution Differences & Cells• solvent + solute = solution
• Hypotonic– Solutes in cell more than outside– Outside solvent will flow into cell
• Isotonic– Solutes equal inside & out of cell
• Hypertonic– Solutes greater outside cell– Fluid will flow out of cell
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Facilitated Diffusion
• Differentially permeable membrane
• Channels (are specific) help molecule or ions enter or leave the cell
• Channels usually are transport proteins (aquaporins facilitate the movement of
water)• No energy is used
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Process of Facilitated Transport
• Protein binds with molecule
• Shape of protein changes
• Molecule moves across membrane
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Active Transport
• Molecular movement
• Requires energy (against gradient)
• Example is sodium-potassium pump
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Cell Permeability
• Passive transport is carrier mediated– Facilitated diffusion– Solute molecule combines with a “carrier” or
transporter– Electrochemical gradients determines the
direction– Integral membrane proteins form channels
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Crossing the Membrane
• Simple or passive diffusion
• Passive transport– Channels or pores
• Facilitated transport– Assisted by membrane-floating proteins
• Active transport pumps and carriers– ATP is required– Enzymes and reactions may be required
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Modes of Transport
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Carrier-Mediated Transport• Integral protein binds to the solute and undergo
a conformational change to transport the solute across the membrane
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Channel Mediated Transport• Proteins form aqueous pores allowing specific solutes to pass across the membrane• Allow much faster transport than carrier proteins
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Coupled Transport
• Some solutes “go along for the ride” with a carrier protien or an ionophore
Can also be a Channel coupled transport
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• Three main mechanisms:– coupled carriers: a solute is
driven uphill compensated by a different solute being transported downhill (secondary)
– ATP-driven pump: uphill transport is powered by ATP hydrolysis (primary)
– Light-driven pump: uphill transport is powered by energy from photons (bacteriorhodopsin)
Active Transport
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Active Transport• Energy is required
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Endocytosis
• Movement of large material– Particles– Organisms – Large molecules
• Movement is into cells
• Types of endocytosis– bulk-phase (nonspecific)– receptor-mediated (specific)
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Process of Endocytosis
• Plasma membrane surrounds material
• Edges of membrane meet
• Membranes fuse to form vesicle
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Forms of Endocytosis
• Phagocytosis – cell eating
• Pinocytosis – cell drinking
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Exocytosis
• Reverse of endocytosis
• Cell discharges material
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Exocytosis
• Vesicle moves to cell surface
• Membrane of vesicle fuses
• Materials expelled
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Endocytosis and Exocytosis
• Exocytosis
- membrane vesicle fuses with cell membrane, releases enclosed material to extracellular space.
• Endocytosis
- cell membrane invaginates, pinches in, creates vesicle enclosing contents
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End Chapter 5
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Types of Proteins
1) Enzymes – catalyzes covalent bond breakage or formation
2) Structural – collagen, elastin, keratin, etc.
3) Motility – actin, myosin, tubulin, etc.
4) Regulatory – bind to DNA to switch genes on or off
5) Storage – ovalbumin, casein, etc.
6) Hormonal – insulin, nerve growth factor (NGF), etc.
7) Receptors – hormone and neurotransmitter receptors
8) Transport – carries small molecules or irons
9) Special purpose proteins – green fluorescent protein, etc.
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Peroxisome 1
• a small organelle that is present in the cytoplasm of many cells and that contains the reducing enzyme catalase and usually some oxidases.
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Peroxisome 2
• A major function of the peroxisome is the breakdown of very long chain fatty acids through beta-oxidation. In animal cells, the very long fatty acids are converted to medium chain fatty acids, which are subsequently shuttled to mitochondria where they are eventually broken down to carbon dioxide and water. In yeast and plant cells, this process is exclusive for the peroxisomes.
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THE END
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