BIO2135_Lab 1_2012
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Transcript of BIO2135_Lab 1_2012
Université d’Ottawa / University of Ottawa
BIO2135 Animal Form and Function1
Lab orientation, magnification and Lab orientation, magnification and surface to volume ratio in animalssurface to volume ratio in animalsLab orientation, magnification and Lab orientation, magnification and surface to volume ratio in animalssurface to volume ratio in animals
BIO2135 Animal Form and Function
Université d’Ottawa / University of OttawaUniversité d’Ottawa / University of Ottawa
BIO2135 Animal Form and Function2
Today’s objectivesToday’s objectivesToday’s objectivesToday’s objectives Logging on and off the computers BIOLabo– Setting up bright field alignment
(Compound microscope)– Setting up the stereoscope
Image preview and capture (Image J software)
Magnification– Fields of view
Logging on and off the computers BIOLabo– Setting up bright field alignment
(Compound microscope)– Setting up the stereoscope
Image preview and capture (Image J software)
Magnification– Fields of view
BIO2135 Animal Form and Function
Université d’Ottawa / University of OttawaUniversité d’Ottawa / University of Ottawa
BIO2135 Animal Form and Function3
Objectives (Cont.)Objectives (Cont.)Objectives (Cont.)Objectives (Cont.) Understand factors affecting diffusion
rates. Understand how surface to volume ratio
is affected by the size of the animal Present quantitative data with a graph
and caption Understand the role of data
transformation, display error in measurements
Gather and report the information
Understand factors affecting diffusion rates.
Understand how surface to volume ratio is affected by the size of the animal
Present quantitative data with a graph and caption
Understand the role of data transformation, display error in measurements
Gather and report the information
BIO2135 Animal Form and Function
Université d’Ottawa / University of OttawaUniversité d’Ottawa / University of Ottawa
BIO2135 Animal Form and Function4
Administrative informationAdministrative informationAdministrative informationAdministrative information
Contact information Care and proper storage of
microscopes
Contact information Care and proper storage of
microscopes
BIO2135 Animal Form and Function
Université d’Ottawa / University of OttawaUniversité d’Ottawa / University of Ottawa
BIO2135 Animal Form and Function5
Science networkScience networkScience networkScience network
Digital Diversity – open your copy Applications Launcher–BIOLabo
–Digital Zoology for when needed
–BIO2135 Website– ImageJ
Digital Diversity – open your copy Applications Launcher–BIOLabo
–Digital Zoology for when needed
–BIO2135 Website– ImageJ
BIO2135 Animal Form and Function
Université d’Ottawa / University of OttawaUniversité d’Ottawa / University of Ottawa
BIO2135 Animal Form and Function6
MagnificationMagnificationMagnificationMagnification
BIO2135 Animal Form and Function
Université d’Ottawa / University of OttawaUniversité d’Ottawa / University of Ottawa
BIO2135 Animal Form and Function7
Magnification Magnification specimensspecimensMagnification Magnification specimensspecimens
Tetrahymena Spirostomum Planaria Liver fluke
(Fasciola hepatica) Earthworm Sea urchin Perch Turtle Yourself
Tetrahymena Spirostomum Planaria Liver fluke
(Fasciola hepatica) Earthworm Sea urchin Perch Turtle Yourself
Measurement Measurement specimensspecimens
Measurement Measurement specimensspecimens
Taenia pisiformis Amphioxus Blepharisma Aurelia ephyra Crayfish Planaria Liver fluke (Fasciola hepatica) Paramecium
Taenia pisiformis Amphioxus Blepharisma Aurelia ephyra Crayfish Planaria Liver fluke (Fasciola hepatica) Paramecium
BIO2135 Animal Form and Function
Université d’Ottawa / University of OttawaUniversité d’Ottawa / University of Ottawa
BIO2135 Animal Form and Function8
Saving a Picture: Dos and Don’tsSaving a Picture: Dos and Don’tsSaving a Picture: Dos and Don’tsSaving a Picture: Dos and Don’ts Save file to K: drive in folder for Bio2135A2
My Documents on ‘sci.uottawa.ca\homes\Undergrads\bio312d4’(K:)
Save only as .jpgOther file formats will not be transferred
DON’T create subfolders in the Bio2135A2 foldere.g. contents Bio2135A2\Lab 1 will NOT be transferred!
Save file to K: drive in folder for Bio2135A2 My Documents on ‘sci.uottawa.ca\homes\Undergrads\bio312d4’(K:)
Save only as .jpgOther file formats will not be transferred
DON’T create subfolders in the Bio2135A2 foldere.g. contents Bio2135A2\Lab 1 will NOT be transferred!
BIO2135 Animal Form and Function
Université d’Ottawa / University of OttawaUniversité d’Ottawa / University of Ottawa
BIO2135 Animal Form and Function9
Measuring surface Measuring surface to volume ratiosto volume ratiosMeasuring surface Measuring surface to volume ratiosto volume ratios
All measurements in mm!Data recorded in spreadsheeton the designated computers
BIO2135 Animal Form and Function
Université d’Ottawa / University of OttawaUniversité d’Ottawa / University of Ottawa
BIO2135 Animal Form and Function10
Microscopy measurementMicroscopy measurementMicroscopy measurementMicroscopy measurement Snap and save your picture using image J.
Open ruler in imageJ. Be sure both are at the same zoom.
Snap and save your picture using image J. Open ruler in imageJ. Be sure both are at the same zoom.
40x
BIO2135 Animal Form and Function
Université d’Ottawa / University of OttawaUniversité d’Ottawa / University of Ottawa
BIO2135 Animal Form and Function11
Thought provoking questions for Thought provoking questions for discussiondiscussionThought provoking questions for Thought provoking questions for discussiondiscussion
What things are moved (exchanged) across the surfaces of organisms?
Where are the gas, nitrogenous waste, and nutrient exchange surfaces in each organism?
Gas exchange requires a moist permeable surface how do organisms balance this requirement with water loss and how does where they live affect this?
What prevents stagnation at the exchange surface? Are there ways to increase the concentration of the material
being exchanged either inside or outside the animal?
What things are moved (exchanged) across the surfaces of organisms?
Where are the gas, nitrogenous waste, and nutrient exchange surfaces in each organism?
Gas exchange requires a moist permeable surface how do organisms balance this requirement with water loss and how does where they live affect this?
What prevents stagnation at the exchange surface? Are there ways to increase the concentration of the material
being exchanged either inside or outside the animal?
BIO2135 Animal Form and Function
Université d’Ottawa / University of OttawaUniversité d’Ottawa / University of Ottawa
BIO2135 Animal Form and Function12
TimelineTimelineTimelineTimeline
Dr. Houseman’s Prelab (10 min) Administrative Prelab (15 min) TA Prelab (15 min) Biolabo and magnification (50 min) Surface to volume (30 min) Group discussion (10 min) Class discussion (30 min)
Dr. Houseman’s Prelab (10 min) Administrative Prelab (15 min) TA Prelab (15 min) Biolabo and magnification (50 min) Surface to volume (30 min) Group discussion (10 min) Class discussion (30 min)
BIO2135 Animal Form and Function
Université d’Ottawa / University of OttawaUniversité d’Ottawa / University of Ottawa
BIO2135 Animal Form and Function13
Graph Assignment using class dataGraph Assignment using class data(You plot all individual data points– each student submits a (You plot all individual data points– each student submits a report, this is not a team assignment)report, this is not a team assignment)
Graph Assignment using class dataGraph Assignment using class data(You plot all individual data points– each student submits a (You plot all individual data points– each student submits a report, this is not a team assignment)report, this is not a team assignment) Graph #1- Surface Area as a function of volume
Graph #2 - Log10 (Surface Area) as a function of Log10 (volume). Slope, standard error, 95% confidence calculations and conclusion.
Graph #3 - Log10 (Surface Area:Volume) as a function of Log10 (volume). Slope, standard error, 95% confidence calculations and conclusions.
Graph #1- Surface Area as a function of volume
Graph #2 - Log10 (Surface Area) as a function of Log10 (volume). Slope, standard error, 95% confidence calculations and conclusion.
Graph #3 - Log10 (Surface Area:Volume) as a function of Log10 (volume). Slope, standard error, 95% confidence calculations and conclusions.
BIO2135 Animal Form and Function
Université d’Ottawa / University of OttawaUniversité d’Ottawa / University of Ottawa
BIO2135 Animal Form and Function14
ReportReportReportReport Data and instructions will be
provided January 23, 2012 on the Bio2135 web site (Excel and pdf files).
Due date will be January 30, 2012 before 4:00 pm in the document drop-off room box for your lab section.
Data and instructions will be provided January 23, 2012 on the Bio2135 web site (Excel and pdf files).
Due date will be January 30, 2012 before 4:00 pm in the document drop-off room box for your lab section.