Arun_Kallur Purushothaman_visualcv_resume2016
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Bangalore KA, INDIA +91 809-502-4477 [email protected] in.linkedin.com/in/arunpurushothamank scholar.google.co.in/citations?user=_NT2OCMAAAAJ&hl=en researchgate.net/profile/Dr_Arun_Kp Senior Research Scientist ✉ Summary and Research Interest Summary and Research Interest Organized Researcher with Ph.D., specialized in Bioanalytical chemistry, biochemistry, pharmacology, immunology assay development, small molecule characterization, cell-based assays, material characterization, phytochemistry and genomics with overall 10 years of laboratory research experience. Accomplished in generating and applying new protocols and technologies. Well experienced in managing teams comprised of scientists and research technicians working to advance industry knowledge. Adept and successful in writing grant proposals and manuscripts. Research Experience Research Experience Traditional Anti-cancer drug source" Key working area: Cancer research, Inflammation and Immunomodulation, Bio-analytical. Characterization of Plant secondary metabolites using UHPLC-MSMS (micrOTOF-Q II, Bruker) LC-MS/MS method development for small molecule and proteomics analysis Proteomic studies through 2D electrophoresis, trypsin in-gel digestion followed by identification of protein using LC-MSMS and MALDI In silico based binding affinity simulation and QSAR studies using Schrodinger Suite Studying anticancer, immunomodulatory and anti-inflammatory efficacy of various plant drug on cancerous mice/rat model Maintenance of various cell lines (EAC, HUVEC, Vero, A431 & CaCO2) Cell Based Bio-Assay (Human / Mice) to determine the efficacy of phytomolecules Western Blotting Cell Lysate and Cytokine profiling – ELISA for protein expression studies Also gained hands on experience in characterization techniques such as FT-IR, CHNO analyzer, HPTLC, NMR, AAS, ICP-MS Industrial Research Experience Industrial Research Experience Bangalore, KA, INDIA Key Research area: Cancer Biology. Bangalore, KA, INDIA Key working area: Cancer (Breast) research, Genomics, Biosimilars, Clinical trial database 1. Establishment of Genomic laboratory: PCR-SSP, RFLP-PCR and gene sequencer 2. LC-MSMS analysis of metabolites in serum sample 3. Cell culture facility infrastructure establishment 1. Designed and directed experiments using multiple cell based and molecular techniques. 2. Processing of Breast cancer FFPE block samples for RNA/DNA extraction. 3. Performing cDNA conversion and qRT-PCR using SYBR Green and TaqMan assay. 4. qRT-PCR assay development and validation. Arun KP Ph.D. Doctorate SASTRA University, TN, INDIA http://sastra.edu/ "Standardization and Biochemical evaluation studies on Jatropha tanjorensis J.L. Ellis & Saroja - A Senior Research Scientist OncoStem Diagnostics Pvt Ltd Scientist-I Avesthagen Pvt Ltd Jul 2015 - Present Nov 2014 - Jun 2015
Transcript of Arun_Kallur Purushothaman_visualcv_resume2016
Bangalore KA, INDIA +91 809-502-4477 [email protected]
in.linkedin.com/in/arunpurushothamank scholar.google.co.in/citations?user=_NT2OCMAAAAJ&hl=en researchgate.net/profile/Dr_Arun_Kp
Senior Research Scientist
✉
Summary and Research InterestSummary and Research Interest
Organized Researcher with Ph.D., specialized in Bioanalytical chemistry, biochemistry, pharmacology,immunology assay development, small molecule characterization, cell-based assays, materialcharacterization, phytochemistry and genomics with overall 10 years of laboratory researchexperience. Accomplished in generating and applying new protocols and technologies. Well experiencedin managing teams comprised of scientists and research technicians working to advance industryknowledge. Adept and successful in writing grant proposals and manuscripts.
Research ExperienceResearch Experience
Traditional Anti-cancer drug source"
Key working area: Cancer research, Inflammation and Immunomodulation, Bio-analytical.
Characterization of Plant secondary metabolites using UHPLC-MSMS (micrOTOF-Q II, Bruker)LC-MS/MS method development for small molecule and proteomics analysisProteomic studies through 2D electrophoresis, trypsin in-gel digestion followed by identification ofprotein using LC-MSMS and MALDIIn silico based binding affinity simulation and QSAR studies using Schrodinger SuiteStudying anticancer, immunomodulatory and anti-inflammatory efficacy of various plant drug oncancerous mice/rat modelMaintenance of various cell lines (EAC, HUVEC, Vero, A431 & CaCO2)Cell Based Bio-Assay (Human / Mice) to determine the efficacy of phytomoleculesWestern Blotting Cell Lysate and Cytokine profiling – ELISA for protein expression studiesAlso gained hands on experience in characterization techniques such as FT-IR, CHNO analyzer, HPTLC,NMR, AAS, ICP-MS
Industrial Research ExperienceIndustrial Research Experience
Bangalore, KA, INDIA
Key Research area: Cancer Biology.
Bangalore, KA, INDIA
Key working area: Cancer (Breast) research, Genomics, Biosimilars, Clinical trial database
1. Establishment of Genomic laboratory: PCR-SSP, RFLP-PCR and gene sequencer2. LC-MSMS analysis of metabolites in serum sample3. Cell culture facility infrastructure establishment
1. Designed and directed experiments using multiple cell based and molecular techniques.2. Processing of Breast cancer FFPE block samples for RNA/DNA extraction.3. Performing cDNA conversion and qRT-PCR using SYBR Green and TaqMan assay.4. qRT-PCR assay development and validation.
Arun KP
Ph.D. DoctorateSASTRA University, TN, INDIA http://sastra.edu/
"Standardization and Biochemical evaluation studies on Jatropha tanjorensis J.L. Ellis & Saroja - A
Senior Research ScientistOncoStem Diagnostics Pvt Ltd
Scientist-IAvesthagen Pvt Ltd
Jul 2015 - Present
Nov 2014 - Jun 2015
1. Separation of Lymphocytes from patients with Autoimmune Disorders specially RheumatoidArthritis (RA), Psoriasis (PSO)
2. Cell surface antigen expression studies (FACS- Beckman Coulter Gallios)3. Cell culture maintenance (HUVEC, Melanocyte, Nb2, HUT78, Daudi, SKBr-3, JURKAT, A431 & CHO)4. Cell Based Bio-Assay (Human / Mice) / MLR / ADCC / 7ADD, CFSE5. Antibody Binding Assay6. Neutralizing antibody Assay7. Western Blotting (phospho tyrosine) / Cell Lysate8. Cytokine profiling – CBA Flow Cytometer based assay9. Anti-lymphocyte antibody Assay (Rosette inhibition/compliment lysis)
Puducherry, INDIA
Project worked:
2. ICMR-INSERM funded Research Project “Studies on T cell vβ repertoire in autoimmuneRheumaticdisorder: Correlation with HLA profile”
Trained in Foreign Lab (FRANCE): Spectratyping Gene Length Analysis Technique: Dr. Srini V Kaveri’s, Lab,UMRS 872 (ex 681), INSERM, Paris.
Role Description:-
Separation of Lymphocytes from patients with Autoimmune Disorders (Systemic Lupus Erythromatosis(SLE), Rheumatoid Arthritis (RA), MAS, GBS, Vasculitis, TB).
Performing HLA typing.Performing Spectratyping / Immunoscope (Paris, FRANCE).Carrying out Cell culture work.DNA / RNA extraction and cDNA synthesis.Study of Oxidative Stress in autoimmune diseases
Vellore, TN, INDIA
Role Description:-
1. Class I (A&B) and Class II (DR & DQ) typing. About 2500 typing a year using Microlymphocytoxicityand PCR-SSP (Low & High Resolution) method
Vellore, TN, INDIA
Course subject handled: Molecular Biology & Immunology.
Worked as Lab. Instructor and helped students in their academic research projects.
Education
1. CCRH-MOHWF funded Research Project “Immunological studies in Rheumatoid Arthritis treated withHomeopathic drugs”.
2. Lymphocyte separation and DNA Isolation3. HLA B27 correlation studies with autoimmune diseases4. Cross matches – Auto, Routine, Extended, Split T & B cell, Monocyte CxM.5. Detection of Cold Antibodies.6. Coaching students in Various Modern Biochemical and Microbiological Techniques.
Christian Medical College and Hospital
ScientistBiocon Pvt Ltd
Senior Research FellowJIPMER
Senior Demonstrator
Guest LecturerAdhipara Sakthi College of Arts and Science
Bangalore, KA, INDIA
Key working area: Cancer (Head & Neck/Blood cell/Breast) research, Autoimmunity, Biosimilars.
Apr 2009 - Aug 2010
Jul 2007 - Mar 2009
Aug 2006 - Jun 2007
Jul 2006 - Jul 2006
EducationEducation
Ph.D. DoctorateSASTRA UniversityThanjavur, TN, INDIA
Key working area: Cancer research, Inflammation and Immunomodulation, Bio-analytical.
Worked in NABL accredited Laboratory.
M.Sc. Biochemistry, University Rank holder – Silver MedalAdhipara Sakthi College of Arts and Science, Thiruvalluvar UniversityVellore, TN, INDIA
B.Sc. Biochemistry, 68%Islamiah College University of MadrasVellore, TN, INDIA
PublicationsPublications
3. Arun, K.P., Rajesh, S.S. Sankaranarayanan, M.S., Sivaraman, T., Brindha, P. Structuralcharacterizations of lead anti-cancer compounds from the methanolic extract of Jatrophatanjorensis. Bangladesh Journal of Pharmacology. 2014; 9: 452-65
4. Hasthi, V. Annapurna., Babu, Apoorvaa., Ravichandran, N., Arun, K.P., Brindha, P., Swaminathan, S.,Vijayalakshmia, M., Nagarajan, A. Isolation and in silico evaluation of antidiabetic molecules ofCynodon dactylon (L.). Journal of Molecular Graphics and Modelling. 39 (2013) 87–9
6. Arun, K.P., Balaji, P., Sivashankari, P.R., David Raj, C., Brindha, P. Proteomics and its RelatedBiological Activity of Jatropha tanjorensis Ellis & Saroja: An Ethnomedicinal Plant. Asian Journal ofChemistry, Vol. 26, No. 9 (2014).
7. Balaji, K., Arun, K.P., Brindha, P., Sridharan, K., UmaMaheswari, K., Swaminathan, S., and Rajan, K.S.Bhanupaka: A Green Process in the Preparation of an Indian Ayurvedic Medicine, Lauha Bhasma.Journal of Chemistry. 2013 1-8
8. Surya, N., Ravichandran, N., Arun, K.P., Brindha, P., Sridharan, K., Rajan, K.S., Uma, K andSwaminathan, S. Botanical standardization studies on Tamarindus indica-bark. Int J Pharm PharmSci. Suppl 1, 6 (2014) 70-72
9. Bino, K., Arun, K.P., Stalin, S., Brindha, P. Stereospermum tetragonam (DL) as an anti-diabetic agentby activating PPARγ and GLUT4. Bangladesh Journal of Pharmacology. 2014; 9: 250-256
10. Krithika, S.S., Shabir, A.G., Arun, K.P., Vijayalakshmi M. In silico and In vitro evaluation of the anti-inflammatory potential of Centratherum punctatum Cass-A. Journal of biomolecular Structure &Dynamics. 2016
11. Arun, K.P., Metabolomics – A new approach in plant biotechnology. Research on Biotechnology inIndia: Some Initiatives and Accomplishments. Oxford & IBH Publishing Co. Pvt. Ltd. InternationalBook Distributing Co. Scientific Publishers (India). ACCEPTED – BOOK ARTICLE
Link to Complete list of Articles published: 20 https://scholar.google.co.in/citations?user=_NT2OCMAAAAJ&hl=en
5. Arun, K.P. and Brindha, P. Investigations into the Phenolic and Alkaloid Constituents of Jatrophatanjorensis by LC-MS/MS and Evaluating its Bioactive Property. Asian Journal of Chemistry. 2015;27(9): 3249-3253
2. Arun, K.P. and Brindha, P. UHPLC–UV–ESI-micrOTOF-Q II analysis of flavonoid fractions fromJatropha tanjorensis. Pharmacognosy Magazine. 10 (39) 472-479 2014
1. Arun, K.P. and Brindha, P. Jatropha tanjorensis extract induces apoptosis and inhibits tumor in vivo. Journal of Pharmacy and Pharmacology. UNDER REVIEW
2010 - 2015
2004 - 2006
2001 - 2004
