20131127 Taller H2020_Proyecto BEADS-ANFACO-CECOPESCA
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Transcript of 20131127 Taller H2020_Proyecto BEADS-ANFACO-CECOPESCA
Seventh Framework Programme
Research for SMEs 2 years: 2011-2013 Budget: 1,112.388.65 €
SEMs RTDs
North Bay Shellfish Ltd
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4.000
6.000
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12.000
14.000
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2.000.000
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6.000.000
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1990 1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001 2002 2003 2004 2005 2006 2007 2008 2009 2010
Valu
e (m
illio
n U
SD)
Prod
uctio
n (t
)
Aquaculture
Capture
Aquaculture, value
Global Bivalve Production
1. TOXIC EPISODES
In 2010 the costs of HABs in Galicia were estimated in 40,000,000 € (Research project purgademar) http://www.purgademar.com
In Europe, estimation of the economic impact of HABs on farmed shellfish was $ 754 Million from 2000 to 2009 which was an annual value of $ 75.4 Million (FAO)
2. MICROBIAL AND PARASITIC CONTAMINATION
Bacteria: E coli, Salmonella spp, L monocytogenes, Vibrio sp Virus: HAV , MNV (winter vomiting bug) Parasites: Bonamia ostreae
List of Work packages (WPs)
WP1: Investigate the use of detoxifying bacteria delivered by microencapsulation to sites of toxins in shellfish WP2: Investigate the use of administered microcapsules as an aid to effective and efficient depuration of pathogens WP3: Investigate the use of immunostimulants or delivery of direct anti-Bonamia agents WP4: Demonstration of results of detoxification/depuration at commercial depuration plants WP5: Evaluation. Assessment of IPR, Dissemination activities and Training WP6: Project Management
WP1: Investigate the use of detoxifying bacteria delivered by microencapsulation to sites of toxins in shellfish
Objectives: 1. To characterize and identify toxin
degrading bacteria
2. To determine properties that may significantly influence degradation of algal toxins by bacteria
3. To develop a “probiotic” diet of toxin degrading bacteria and a microencapsulation delivery system for shellfish consumption with targeted release in the DG to degrade toxins
Objectives
1. To design protocols using fluorescent microbeads to approve the suitability of shellfish purification systems
2. To define a panel of bacteria with potential activity against pathogens
3. To determine the best method for microencapsulation of selected bacteria
4. To investigate viability of applying microencapsulated diets to increase the depuration process of shellfish from pathogens
WP2: Investigate the use of administered micro-capsules as an aid to effective and efficient depuration of pathogens
WP3: Investigate the use of immunostimulants or delivery of direct anti-Bonamia agents
Objectives
1. Determine the size and types of microencapsulated
beads to use in oysters tissues
2. Optimize the delivery of microencapsulated beads
or immunostimulats to oysters infected with B ostreae
3. To determine if those enhance the immune
response of the infected oyster
4. To prove that there is a reduction in the prevalence
or intensity of infection
WP 4: Demonstration of results of detoxification/depuration at commercial depuration plants
Objectives
• To determine the potential of probiotic diets to detoxify shellfish in a commercial setting.
• To design “alternative” protocols using fluorescent microbeads (as a proxy for bacteria/virus) for shellfish depuration system approval.
• To determine the increase of the efficiency of shellfish depuration of bacteria and viruses using microencapsulated probiotics.
WP5: Evaluation. Assessment of IPR, Dissemination activities and Training WP6: Project Management
Objectives
1. To evaluate the results of the project
2. Dissemination and Exploitation of the results
3. To train key personnel in the SMEs and others
4. To manage the project: meetings, tasks, financial administration
Main Common Tasks WP1 and WP2 1. Isolation and characterization of toxin or pathogens degrading bacteria focusing on degradation of: ASP and DSP toxins Bacteria and Virus 2. Define the best method for construction of microcapsules-containing bacteria 3. Delivery of microencapsulated “probiotic” diets in the DG of shellfish to improve depuration and toxin degradation
MAIN ACHIEVMENTS OF THE BEADS PROJECT A protocol using fluorescent microbeads was designed to substitute the microbiological challenge test Almost 1000 bacteria isolated from shellfish and screened for activity against pathogens or toxins Some isolates, as bacteriocin-like substances show activity against L monocytogenes or E coli Compounds as zymosan microencapsulated present an inmunoestimulant effect on oysters
MAIN ACHIEVMENTS OF THE BEADS PROJECT
Some bacteria have potential to degrade toxins: OA and DA A method for microencapsulation and assessment of incorporation of bacteria or isolates into the DT of shellfish was developed Alginate capsules are a useful tool to transport probiotic bacteria and release them into de DG During passage through the intestine, the alginate beads are broken, so bacteria are released into the DT
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MAIN ACHIEVMENTS OF THE BEADS PROJECT
Alginate capsules can be used for fluorescent beads transport to the DG. These can be
monitored during their incorporation and later release in the DG of mussels and oysters and
then used in challenge tests.
Challenge test
Alginate capsules containing non degradable fluorescent dye microbeads in faeces of mussels.
MAIN ACHIEVMENTS OF THE BEADS PROJECT
Selection and microencapsulation of suitable probiotic bacteria or extracts
1. Encapsulation of 3M.21 In vitro activity:
2. Encapsulation of 3M18 Anti MNV-1 in vitro
3. Encapsulation of 4O11 Anti MNV-1 in vitro
Anti Listeria
Anti E. faecalis Anti Hepatitis A Anti MNV-1 activity
MAIN ACHIEVMENTS OF THE BEADS PROJECT
Delivery of beads containing probiotics to the target shellfish
• Alginate capsules transport probiotic bacteria and release them into DGs since they have been observed by light microscopy as intact in the DG of shellfish during feeding period. • Broken alginate beads were found in faeces, so during passage through the intestine bacteria embedded by alginate capsules are released.
•Probiotic diets embedded by alginate capsules can be released to improve depuration
Alginate capsules, empty and with a probiotic bacteria.
WP4. Demonstration of results of detoxification/depuration
at commercial depuration plants
Objectives
• To determine the potential of probiotic diets to detoxify shellfish in a commercial setting.
• To design “alternative” protocols using fluorescent microbeads (as a proxy for bacteria/virus) for shellfish depuration system approval.
• To determine the increase of the efficiency of shellfish depuration of bacteria and viruses using microencapsulated probiotics.
Months 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
WP4. Demonstration of results of detoxification/depuration at commercial depuration plants
Degradation of microalgal toxins
Depuration of bacteria/viruses
Challenge test
Assessment of: Toxin concentrations Microbeads clearance rates Bacterial and viral clearance rates
Microbeads Demonstration at commercial depuration plants
Report on demonstration of the challenge test
WP4. Demonstration of results of detoxification/depuration
at commercial depuration plants
Report on demonstration of the challenge test
WP4. Demonstration of results of detoxification/depuration
at commercial depuration plants
Conclusion: Microencapsulated probiotic bacteria can reach and colonize the DG of shellfish and then improve shellfish depuration Outcomes of this project belong to the industries of the consortium. They decided to put together some 'heads of terms' which is the basis for a joint venture or commercial collaboration in order to exploit the results reached in the project
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North Bay Shellfish Ltd
Thanks for your atention