¨±±³ ¯³¯§‡‡§ Photometry

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بررسي دستگاههاي Photometry. Spectrophotometers Flame Photometers. دستگاه Spectrophotometer :. شامل دو بخش اصلي است: اسپكتروسكوپ فوتومتر. دستگاه Spectrophotometer :. امواج الكترومغناطيس:. Electomagnetic Spectrum: max frequency (Hz ) wavelength 10 3 RF10 6> 1 km - PowerPoint PPT Presentation

Transcript of ¨±±³ ¯³¯§‡‡§ Photometry

  • PhotometrySpectrophotometers Flame Photometers

  • Spectrophotometer : :

  • Spectrophotometer :

  • :Electomagnetic Spectrum:max frequency (Hz) wavelength 103RF106> 1 km 107AM107500 m - 50 m 107TV,FM10910 m - 1 m 1010microwave101210 cm - 1mm 1012infra-red3.9x10141 mm - 720 nm 3.9x1014visible7.5x1014720 nm - 390 nm 7.5x1014ultra-violet1017390 nm - 1 nm 1016x-rays 102010 nm - .5 pm 1019gamma-rays< 10 pm

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  • : IR Bands:visibleWavelength (l)

  • : Wavelength (l)UV is energetic enough to break covalent bonds. Causes sunburn.

  • : V = Wave Number (cm-1)l = Wave LengthC = Velocity of Radiation (constant) = 3 x 1010 cm/sec. u = Frequency of Radiation (cycles/sec)

    The energy of photon: h (Planck's constant) = 6.62 x 10-27 (Ergsec)C = u

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  • Beer-Lambert : Beer-Lambert .

    P0 : P : a : ( ) L : C : (a) .

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  • : : 200 360 .90 . : 360 800 . .

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  • :UV Spectrophotometer1.Hydrogen Gas Lamp2.Mercury LampVisible Spectrophotometer1.Tungsten LampIR Spectrophotometer1.Carborundum (SIC)

  • : . ( 10% - H-B-W = 50 nm ) ( 40-60% - H-B-W = 10-15 nm ) ( 90% - H-B-W = 4 nm )

  • : ( Monochromators ) : ( H-B-W= 0.5 nm) ( 350 ) .. . ( H-B-W= 0.5 nm) . .. .

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  • : (Voltaic Photo Cell) Selenium Silicon 400-800 nm . ( Photo Multiplier Tube )

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  • :UV SpectrophotometerQuartz (crystalline silica)Visible SpectrophotometerGlassIR SpectrophotometerNaCl

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  • : Holder : : .

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  • : Beer-Lambert ( ) . .

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  • Flame Photometer : : . . ( Ground State ) . . .

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  • ( aerosol) .

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