化脓菌分离鉴定过程(二) 肠道菌分离鉴定过程(二)
-
Upload
akeem-hicks -
Category
Documents
-
view
301 -
download
0
description
Transcript of 化脓菌分离鉴定过程(二) 肠道菌分离鉴定过程(二)
![Page 1: 化脓菌分离鉴定过程(二) 肠道菌分离鉴定过程(二)](https://reader030.fdocuments.net/reader030/viewer/2022033006/56813361550346895d9a77af/html5/thumbnails/1.jpg)
化脓菌分离鉴定过程(二)肠道菌分离鉴定过程(二)
Experiment Two
![Page 2: 化脓菌分离鉴定过程(二) 肠道菌分离鉴定过程(二)](https://reader030.fdocuments.net/reader030/viewer/2022033006/56813361550346895d9a77af/html5/thumbnails/2.jpg)
化脓菌分离鉴定过程Objective掌握从临床标本中分离和鉴定化脓性球菌的原则和方法。辅助临床诊断及指导临床用药。
![Page 3: 化脓菌分离鉴定过程(二) 肠道菌分离鉴定过程(二)](https://reader030.fdocuments.net/reader030/viewer/2022033006/56813361550346895d9a77af/html5/thumbnails/3.jpg)
![Page 4: 化脓菌分离鉴定过程(二) 肠道菌分离鉴定过程(二)](https://reader030.fdocuments.net/reader030/viewer/2022033006/56813361550346895d9a77af/html5/thumbnails/4.jpg)
![Page 5: 化脓菌分离鉴定过程(二) 肠道菌分离鉴定过程(二)](https://reader030.fdocuments.net/reader030/viewer/2022033006/56813361550346895d9a77af/html5/thumbnails/5.jpg)
![Page 6: 化脓菌分离鉴定过程(二) 肠道菌分离鉴定过程(二)](https://reader030.fdocuments.net/reader030/viewer/2022033006/56813361550346895d9a77af/html5/thumbnails/6.jpg)
![Page 7: 化脓菌分离鉴定过程(二) 肠道菌分离鉴定过程(二)](https://reader030.fdocuments.net/reader030/viewer/2022033006/56813361550346895d9a77af/html5/thumbnails/7.jpg)
Identified method for pyogenic cocci
Gram-stainIsolation and culturePure culture Direct identification The antibiotic susceptibility test
![Page 8: 化脓菌分离鉴定过程(二) 肠道菌分离鉴定过程(二)](https://reader030.fdocuments.net/reader030/viewer/2022033006/56813361550346895d9a77af/html5/thumbnails/8.jpg)
Gram-stain
Morphological characteristics 葡萄球菌-球形,呈葡萄串状排列链球菌-链状排列肺炎链球菌-矛头状,成双排列,尖端向外
![Page 9: 化脓菌分离鉴定过程(二) 肠道菌分离鉴定过程(二)](https://reader030.fdocuments.net/reader030/viewer/2022033006/56813361550346895d9a77af/html5/thumbnails/9.jpg)
Staphylococci are Gram-positive cocci, typically arranged in clumps or Grape-like clusters
![Page 10: 化脓菌分离鉴定过程(二) 肠道菌分离鉴定过程(二)](https://reader030.fdocuments.net/reader030/viewer/2022033006/56813361550346895d9a77af/html5/thumbnails/10.jpg)
![Page 11: 化脓菌分离鉴定过程(二) 肠道菌分离鉴定过程(二)](https://reader030.fdocuments.net/reader030/viewer/2022033006/56813361550346895d9a77af/html5/thumbnails/11.jpg)
Isolation and culture
Cultural• Specimens planted on blood agar plates
give rise to typical colonies in 18h at 37℃
![Page 12: 化脓菌分离鉴定过程(二) 肠道菌分离鉴定过程(二)](https://reader030.fdocuments.net/reader030/viewer/2022033006/56813361550346895d9a77af/html5/thumbnails/12.jpg)
Isolation and culture colonial characteristics
• 致病性葡萄球菌• 中等大小( 1 ~ 2mm)、表面光滑、边缘整齐、不透明、有溶血环、产生金黄色色素或白色色素
• 乙型溶血性链球菌• 菌落较小( 0.5 ~ 0.75mm)、灰白色、表面光滑、有光泽、有清晰透明溶血环
• 甲型溶血性链球菌 /肺炎链球菌• 细小菌落周围产生草绿色溶血环。
![Page 13: 化脓菌分离鉴定过程(二) 肠道菌分离鉴定过程(二)](https://reader030.fdocuments.net/reader030/viewer/2022033006/56813361550346895d9a77af/html5/thumbnails/13.jpg)
纯培养( Pure culture)Materials
• Agar slope culture• Colonies on agar plate
![Page 14: 化脓菌分离鉴定过程(二) 肠道菌分离鉴定过程(二)](https://reader030.fdocuments.net/reader030/viewer/2022033006/56813361550346895d9a77af/html5/thumbnails/14.jpg)
![Page 15: 化脓菌分离鉴定过程(二) 肠道菌分离鉴定过程(二)](https://reader030.fdocuments.net/reader030/viewer/2022033006/56813361550346895d9a77af/html5/thumbnails/15.jpg)
![Page 16: 化脓菌分离鉴定过程(二) 肠道菌分离鉴定过程(二)](https://reader030.fdocuments.net/reader030/viewer/2022033006/56813361550346895d9a77af/html5/thumbnails/16.jpg)
Specimens Different specimens should be taken depending
on the kind and the process of the disease.
![Page 17: 化脓菌分离鉴定过程(二) 肠道菌分离鉴定过程(二)](https://reader030.fdocuments.net/reader030/viewer/2022033006/56813361550346895d9a77af/html5/thumbnails/17.jpg)
革兰染色
![Page 18: 化脓菌分离鉴定过程(二) 肠道菌分离鉴定过程(二)](https://reader030.fdocuments.net/reader030/viewer/2022033006/56813361550346895d9a77af/html5/thumbnails/18.jpg)
Isolation and culture
Culture medium: S.S agar Method: streak plate
![Page 19: 化脓菌分离鉴定过程(二) 肠道菌分离鉴定过程(二)](https://reader030.fdocuments.net/reader030/viewer/2022033006/56813361550346895d9a77af/html5/thumbnails/19.jpg)
Results: unpathogenetic: middle size, red, opaque Suspect colonies: small, colorless, semiopaque
![Page 20: 化脓菌分离鉴定过程(二) 肠道菌分离鉴定过程(二)](https://reader030.fdocuments.net/reader030/viewer/2022033006/56813361550346895d9a77af/html5/thumbnails/20.jpg)
Primary identification
双糖铁培养
![Page 21: 化脓菌分离鉴定过程(二) 肠道菌分离鉴定过程(二)](https://reader030.fdocuments.net/reader030/viewer/2022033006/56813361550346895d9a77af/html5/thumbnails/21.jpg)
![Page 22: 化脓菌分离鉴定过程(二) 肠道菌分离鉴定过程(二)](https://reader030.fdocuments.net/reader030/viewer/2022033006/56813361550346895d9a77af/html5/thumbnails/22.jpg)
细菌基本形态观察葡萄球菌链球菌淋病奈瑟菌大肠杆菌霍乱弧菌