Post on 27-Jan-2021
Introduction to
BioLumix
MIKROBIOLOGIE
BRAUEREITECHNOLOGIE
ZELLBIOLOGIE
BIOTECHNOLOGIE
I&L BIOSYSTEMS GmbH (D)
1991
The I&L Group
LA BIOSYSTEMS BV (NL)
2007
I&L BIOSYSTEMS FRANCE SAS (F)
2015
I&L BIOSYSTEMS UK Ltd. (UK)
2016
What is BioLumix?
rapid microbiological testing system
growth based – photometric measurement
ready-to-use vials with prepared media
CO2 as universal metabolite
Sensor detects CO2 produced by microorganisms
Separation of incubation and detection
Principle of Detection
Measurement of light intensity
Color change of indicator
Detection by Color Change
Measurement every 6 minutes
Light intensity increases with color change
Increase triggers detection event
Incubation and Monitoring
BioLumix is … simpler
almost no maintenance
intuitive sample preparation
easy to operate
1 min 8h 22 h
Faster results…
…for faster release
Sensitivity = one viable cell per vial
single bacterial cell detects in 8-18 h
single yeast cell detects in 20-30 h
mould cells detect in 35-48 h
Sensitivity
Organism Incubationtemp.
Colony count (CFU)
1,00E+00 1,00E+01 1,00E+02 1,00E+03 1,00E+04 1,00E+05
A. brasiliensis¹ 28 ˚C 32,37 28,12 23,86 19,61 15,35 11,10
A. brasiliensis² 28 ˚C 42,76 37,69 32,61 27,53 22,46 17,38
C. albicans¹ 28 ˚C 26,02 22,54 19,05 15,57 12,08 8,60
C. albicans² 28 ˚C 27,75 23,44 19,13 14,82 10,51 6,20
E. coli¹ 35 ˚C 10,19 9,18 8,18 7,17 6,16 5,16
E. coli² 35 ˚C 12,15 11,04 9,93 8,81 7,70 6,59
P. aeruginosa¹ 35 ˚C 16,51 14,81 13,11 11,40 9,70 8,00
P. aeruginosa² 35 ˚C 17,83 16,08 14,34 12,59 10,84 9,10
S. aureus¹ 35 ˚C 16,55 14,97 13,38 11,79 10,20 8,61
S. aureus² 35 ˚C 16,98 15,32 13,66 12,00 10,34 8,68
Detection times in hours of some selected strains in relation to CFU
1 = pure culture2 = pure culture damaged by product (preservatives)
Detection Times with BioLumix
quadruplicate tests with pure cultures
standard deviation approx. 0.3 h
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High Reproducibility
BioLumix is … convincing
comprehensive validation (USP)
versatile application
practice-approved
hands-on:
1 min / sample
Example:
spec. level
Liquid culture can be used for further tests
Automated monitoring & evaluation
Significant reduction of hands-on time
Semiquantitative Results
White = no growth = compliance with limits
Red = growth = out of specification
Clearance Demand for action
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Test for pathogenic organisms
Examples of neutralizers:
Dilute product 1:10 with neutralizer
Add 0.1-1.0 ml to Biolumix vial and incubate
Use vials with pre-added neutralizers
D/E Neutralizer
Letheen broth
TAT
TSB + Tween und Lecithin
Neutralizing Preservatives
Sample Preparation
Tests for effective neutralization of
preservatives
Control sample and spiked product
must yield similar detection times
Suitability test prevents false
negative tests due to incomplete
neutralizationFigure 4: TAC controls; blue, B.subtilis (10.0h); red, sample
1 spiked (12.1h); cyan, sample 6 spiked (17h); green,
negative control (TSB)
Liquid soap - spiked
Suitability Test
swab the surface
cut of the swab and directly incubate in the Biolumix vial
alternatively: dilute in buffer
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Environmental Monitoring
Total Aerobic Count
Yeast & Mould
Coliforms
Enterobacteriaceae
Heterotrophs (for water)
E. coli
Pseudomonas
Staphylococcus
Salmonella
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Available Assays