Protein Engineering

BIT317 Class 2438

Lecture 7Synthesis and Semi-synthesis of Peptides

Strategy inSolid Phase Peptide Synthesis

Pioneered byMerrifield

Microwave Assisted Synthesis

Reduction in synthesis timeFor peptides that contain beta sheet structures / sterically hindered couplings

Major Limitation of SPPS

Feasibility for application for larger proteins

Higher probability of misfolding

Enhanced error rates

Levels of purity

Formation of disulfide bonds (disulfide scrambling)

Protein semisynthesis protein ligation

Chemoselective ligation for artificial backbone