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CSH

, , , Bonnie A. Alden2, Kerri M.Smith2, Paul D. Rainville2, Thomas H.Walter2, Martin Gilar2

, Waters Corporation

CSH MS

BEHFigure 1

CSH C18 CSH Phenyl-Hexyl CSH Fluoro-Phenyl Figure 2 Table 1

UV pH

CSH

TCA

References 1. P. C. Iraneta, K. D. Wyndham, D. R. McCabe, and T. H. Walter, “A Review of Waters Hybrid Particle Technology. Part 3.

Charged Surface Hybrid (CSH) Technology and Its Use in Liquid Chromatography”, Waters white paper 720003929EN, June 2010 2. N. Lucie, V. Hana and P. Solich, Talanta 93 (2012), 99 3. U. Neue, P. Iraneta, F. Gritti and G. Guiochon, J. Chrom. A 1318 (2013), 72 4. F. Gritti and G. Guiochon, J. Chrom. A 1282 (2013), 46, 58 and 113 5. J. Fiori, E. Amadesi, F. Fanelli, C. V. Tropeano, M. Rugolo and R. Gotti, J. Pharm. Biomed. Analysis 150 (2018), 33

ACQUITY UPLC PDA10 mM pH3-4

0.3 mL/min 300.25 mg/mL

1 μL 70/30220 nm 2.1×50 mm

TCAACQUITY UPLC I-Class Xevo TQ-S TCA

A = 0.1% B = 0.1%B 3 0% 30% B95%

1 7 0.4 mL/min50 100 μM 3 μL

Multiple Reaction Monitoring MRM2 kV 600 1000 L/hr

3 CSH BEHC18 2.4 μmol/m2

pH 3 - 8.5 Figure 3 2

3 CSHCSH C18 CSH Phenyl-Hexyl CSH Fluoro-Phenyl3 CSH

CSH pH 3 pHpH 6

pKa 5 CSH Fluoro-PhenylpH 6

pH 6

CSH pH 5 pH

CSH C18 CSH Phenyl-Hexyl CSH Fluoro- PhenylCSH TCA

TCATCA 2 3

Figure 4 pKa 2.8 - 3.80.1%

CSHACQUITY CSH Phenyl-Hexyl 1.7μm 2.1×100 mm

Figure 5 CSH Phenyl-Hexyl

% C pH CSH C18 2.3 yes 15.5 1 - 11

CSH Phenyl-Hexyl 2.3 yes 13.5 1 - 11 CSH Fluoro-Phenyl 2.3 no 10.2 1 - 8

Figure 3 pH

Figure 4: TCA

Figure 5: BEH C18 CSH C18 CSH Phenyl-Hexyl 1.7μm 2.1×100 mm TCA

Figure 1: CSH

Table 1: CSH

Figure 2: CSH