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Consistent, high-quality separations
Easy handling
Compatible with downstream applications
Innovative products
From lab bench to clinical research applications
MACS TechnologyGold standard in cell separation
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CliniMACS Plus InstrumentThe CliniMACS Systema closed and sterilesystem for separation of large cell samples
From lab bench to clinical research applications
From small samples to samples ranging up to 1011 cells by
using columns with dierent cell loading capacity and their
suitable separators
Automated cell separation: The autoMACS Pro Separator
allows standardization o requent cell separations.
Sterile separations in a closed system: The CliniMACSPlus
Instrument in combination with GMP reagents opens new
opportunities or clinical research.
CD133+ cellsisolated withMACS Technology becameadherent and CD133during cultivation butgave rise to non-adherentCD133+ cells buddingrom the adherent cellsurace by asymmetriccell division
Compatible with downstream applications
Gentle on cells: small MicroBeads in combination
with MACS Column Technology allow the purication
o viable and unctionally active cells.
No inuence on analysis: small MicroBeads do not inuence
ow cytometric or microscopic analysis.
Suitable or cell culture and in vivo experiments: MicroBeads
are biodegradable, the short procedure and gentle
column technology avoid mechanical stress.
Molecular biology experiments: limited amount o small
MicroBeads avoid any inuence on urther experiments.
Innovative products
Continuous product development with ocus on current
research and clinical research needs
T cells: isolation o regulatory T cells, naive and memory/eectorcells, Th2 cells, or even antigen-specic T cells according to their
secretion o cytokines
Stem cells: CD133the marker or isolation o hematopoietic
and nonhematopoietic stem cells
Dendritic cells: unique kits or isolation o myeloid and
plasmacytoid human dendritic cells and mouse dendritic
cell subsets
Spleen cells beore separation Isolated CD4+CD25+ T cells
Plasmacytoid blooddendritic cellsisolated with MACSTechnologyandstained
with May-Grnwald-Giemsa
CD4+CD25+ regulatory T cells isolated rom mouse spleen usingMACS Technology
autoMACS Pro SeparatorWalk-away cell sorting of multiple samples
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MACS TechnologyGold standard in cell separation
Consistent, high quality separations
Optimal purity and recovery due to highly specic reagents
combined with efcient MACS Column Technology
Frequent cells such as T, B, NK cells and monocytes,
but also rare cells like stem cells, antigen-specic T cells,
and dendritic cell subsets
For any cell type: over 200 reagents or the direct isolation
o human, mouse, rat, and non-human primate cells;
any other cell type can be isolated using indirect labeling
with MicroBeads
Reliable and consistent results: based on extensive
experience in cell separation systems and rigorous
quality control
Easy handling
Convenient procedure:
easy-to-use system, applicable in every lab
Fast results: cells can be puried in up to 30 minutes
or positive selection or depletion due to short incubation
time and efcient MACS Column Technology;
no time-consuming bead detachment necessary
For positive selection and depletion strategies:
both labeled and unlabeled ractions can be obtained
with excellent purity and recovery
Prepackaged starting kits available:
consisting o a magnet and stand, columns and reagents
CD4+ T cells isolated using an LSColumn and a MidiMACS Separator
CD4+ T cells isolated usingthe autoMACS Separator
Bone marrow cellsbeore separation Isolated CD34+ cells
Mouse spleen
Human bone marrowMiniMACS SeparatorBasic tool for the lab:MiniMACS Separator with an MS Column
QuadroMACS SeparatorFor the simultaneous separation of 4 samples
The advantages
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Magnetic separation
MACS Column Technologyprovides a high-gradient
magnetic ield.
Gentletocells
Thoroughrinsing
procedure
Highrecovery
Untouched isolation by
depletion o speciic cells
Magnetic labeling
Gentle to cells;
minimal inluence
on downstream
experiments
Elution of the labeled cell fraction
Optimal resultseven or rare cells
by using positive selection
N S
Electron micrographs on this page with courtesy o Pro. Peter Groscurth, Institute o Anatomy, University o Zrich, Switzerland.
MACS TechnologyGold standard in cell separation
MACS Technology
MACS Technology has become the standard
method or cell separation. Numerous
publications have proven its versatility ormultiple applications: cell separations with
consistent high-quality results rom lab bench
to clinical applications, rom small to large
scale, rom requently occurring cells to rare
cells and sophisticated subsets. Miltenyi Biotec
provides researchers worldwide with the tools
or high-quality separations.
The principle
MACS Technology is based on MACS
MicroBeads, MACS Separators, and
MACS Columns. MACS MicroBeads aresuperparamagnetic particles o approximately
50 nanometers in diameter. They are
composed o a biodegradable matrix, and it is
thereore not necessary to remove them rom
cells ater the separation process.
Usually, MACS MicroBeads do not alter
structure, unction, or activity status o labeled
cells and are not known to interere with
subsequent experiments.
MACS Technology takes place within MACS
Columns. When a MACS Column is placed in a
MACS Separator, a strong permanent magnet,
a high-gradient magnetic eld is induced on
the column matrixstrong enough to retain
cells labeled with minimal amounts o MACS
MicroBeads. Unlabeled cells pass through and
can be collected; labeled cells are released
ater removal o the column rom the magnet.
Thus, with MACS Technology both labeled and
unlabeled cell ractions can easily be isolated
with high purity. The entire procedure o
positive selection or depletion takes less than
30 minutes, and cells can immediately be used
or urther experiments.
MACS Cell Separation is based on the use o MACS
MicroBeads, MACS Columns, and MACS Separators.
Cells can easily be purifed or depleted in less than30 minutes.
Anti-Biotin-PE
CD4-FITC
CD34-FITC
CD1
33/2-PE
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Direct labeling is the astest way o magnetic labeling. It
requires only one labeling step since the specic antibody
is directly coupled to the magnetic particle. Direct labelingreduces the number o washing steps and, thereore, avoids
unnecessary cell loss. Miltenyi Biotec provides highly specic
monoclonal antibodies or low background and optimal
separation, targeted against surace markers o cells rom
various species such as human, mouse, rat, and non-human
primates. Fluorochrome-conjugated MACS Antibodies can be
added or the purpose o analyzing magnetically separated cell
ractions by ow cytometry or uorescence microscopy.
Direct magnetic cell labeling Indirect magnetic cell labeling
Indirect labeling is perormed i no direct MicroBeads or
the cell type o interest are available. Almost any monoclonal
or polyclonal antibody targeting any cell type rom anyspecies can be used or indirect labeling. Cells are labeled
with a primary antibody that is unconjugated, biotinylated, or
uorochrome-conjugated. In a second step, magnetic labeling
is perormed by using Anti-Immunoglobulin, Anti-Biotin,
Streptavidin, or Anti-Fluorochrome MicroBeads, respectively.
A cocktail o antibodies can also be used to isolate or deplete a
number o cell types concurrently.
Since indirect labeling amplies the magnetic label, it may be
the method o choice or magnetic separation according to
dimly expressed antigens.
MACS Magnetic Labeling
Antibody MicroBead
Streptavidin MicroBead
Biotinylated antibody
Fluorochrome-conjugated antibody
Anti-Immunoglobulin MicroBead
Streptavidin MicroBead
Anti-Fluorochrome MicroBead
Anti-Biotin MicroBead
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Positive selection strategy Untouched isolation
Positive selection means that the desired target cells are
magnetically labeled and isolated as the magnetically retained
cell raction.
A positive selection strategy should be considered or:
excellent purity, especially or rare cell enrichment,
excellent recovery, and
ast results.
Positive selection is the most direct and specic way to isolate
the target cells rom a heterogenous cell suspension. Binding
o antibodies (which are part o the MicroBeads) to the cell
surace does not aect viability or unction o the cells. Both
ractions, labeled and unlabeled, can be recovered and used.
Due to their composition o iron oxide and polysaccharide,
MicroBeads are biodegradable and typically disappear ater a
ew days in culture.
MACS Separation Strategies
Untouched isolation is perormed by depletion o undesired
cells. Non-target cells are magnetically labeled and eliminated
rom the cell mixture. The non-magnetic, untouched cell ractioncontains the target cells.
Untouched isolation should be considered:
or removal o unwanted cells,
i no specic antibody is available or target cells,
i binding o the antibody to the target cells is not desired, or
or subsequent isolation o a cell subset by means o
positive selection.
For many dierent cell types Miltenyi Biotec oers optimized
MACS Cell Isolation Kits containing pre-titrated cocktails o
antibodies directed against non-target cells.
Magnetic separationUndesired cells are retained in a
MACS Column placed in a MACS
Separator.
The target cells pass through the
column and are collected as the
enriched, unlabeled cell raction,
depleted o non-target cells.
Magnetic labeling
Non-target cells are magnetically
labeled with a biotinylated antibody
cocktail and Anti-Biotin MicroBeads.
Magnetic separationCells are separated in a
MACS Column placed in a
MACS Separator.
The low-through raction can
be collected as negative raction
depleted o the labeled cells.
Magnetic labeling
Cells o interest are magnetically
labeled with MACS MicroBeads.
Elution of the labeled cell fraction
The column is removed rom the
separator. The retained cells are
eluted as the enriched, positively
selected cell raction.
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Sequential sorting (1):Depletion followed by positive selection
Sequential sorting (2):MultiSort strategy
Cell subsets can be isolated by rst depleting the non-target
cells and then positively selecting the cell subsets o interest.
This strategy is useul i undesired cells in the cell suspensionexpress the same antigen which is used or positive selection o
the target cells. For isolation o extremely rare cells, it also can
be useul rst to deplete non-target cells rom the suspension.
Positive selection can then be carried out with the pre-enriched
raction to obtain very pure cells.
With MACS MultiSort Kits high numbers o cells, characterized
by multiple cell surace markers, can be sorted easily. Even rare
cells can be enriched very efciently.Multi-parameter sorting with MACS MultiSort Kits allows
sequential positive selections o cells. The target cells are
rst labeled with MACS MultiSort MicroBeads and positively
selected or the rst parameter. Then the cells are incubated
with the MultiSort Release Reagent which enzymatically
removes the MicroBeads rom the antibodies. In the next
step, the target cells are magnetically labeled with MACS
MicroBeads directed against a subset marker to be positively
selected a second time.
MACS Separation Strategies
1st magnetic labeling
Non-target cells are
magnetically labeled with a
biotinylated antibody
cocktail and Anti-Biotin
MicroBeads.
1st magnetic separation
Undesired cells are
retained in a MACS
Column placed in a
MACS Separator while
the unlabeled cells
pass through.
2nd magnetic labeling
Target cells are magnetically
labeled with MicroBeads
according to a subset marker.
2nd magnetic separation
Target cells are
retained in the column
while unlabeled cellspass through.
Ater the column is removed
rom the separator, the
target cells are eluted as the
enriched, positively selected
cell raction.
1st magnetic labeling
Cells o interest are
magnetically labeled with
MultiSort MicroBeads.
1st magnetic separation
Target cells are magnetically
isolated by positive
selection.
Release of magnetic
particles
MultiSort MicroBeads are
enzymatically released.
2nd magnetic labeling
Cell subset o interest is
labeled with MACS
MicroBeads according to asecond marker.
2nd magnetic separation
Target cells are positively
selected a second time.
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Miltenyi Biotec provides products and services worldwide. Visit www.miltenyibiotec.com/local to nd your nearest Miltenyi Biotec contact.
The CliniMACS System components: Instruments, Reagents, Tubing Sets, and PBS/EDTA Buer are manuactured and controlled under an ISO 13485 certied quality system. In Europe, the CliniMACS Systemcomponents areavailable as CE-marked medical devices. In the USA, the CliniMACS System components including t he CliniMACS Reagents are available or use only un der an approved Investigational New Drug(IND) application or Investigational Device Exemption (IDE). CliniMACS MicroBeads are or research use on ly and not or use in humans. autoMACS and CliniMACS are registered trademarks o Miltenyi Biotec
GmbH. MiniMACS and QuadroMACS are trademarks o Miltenyi Biotec GmbH. Unless otherwise specically indicated, Miltenyi Biotec products and services are or research use only and not or therapeuticor diagnostic use. Copyright 2010 Miltenyi Biotec GmbH. All rights reserved.
130-091-618.0
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Miltenyi Biotec oers a wide variety o products or magnetic
cell isolation in basic research as well as or clinical-grade cellseparationsproviding the perect tools or translational
research. The continuously growing portolio also includes
instrumentation and numerous antibodies or ow cytometry,
as well as products or sample preparation, cell activation and
expansion, and molecular analysis.
To nd out more, visit our homepage at
www.miltenyibiotec.com
Germany/Austria/SwitzerlandMiltenyi Biotec GmbHFriedrich-Ebert-Strae6851429 Bergisch GladbachGermanyPhone +49 2204 8306-0
Fax +49 2204 [email protected]
USA/CanadaMiltenyi Biotec Inc.2303 Lindbergh StreetAuburn, CA 95602, USAPhone 800 FOR MACSPhone +1 530 888 8871Fax +1 530 888 [email protected]
www.miltenyibiotec.com
AustraliaMiltenyi BiotecAustralia Pty. Ltd.Unit 16A, 2 Eden Park DriveNorth Ryde, NSW 2113AustraliaPhone +61 2 8877 7400
Fax +61 2 9889 [email protected]
BeneluxMiltenyi Biotec B.V.Postbus 85183, 3508 AD UtrechtThe [email protected]
Customer service NetherlandsPhone 0800 4020120Fax 0800 4020100Customer service BelgiumPhone 0800 94016Fax 0800 99626Customer service LuxembourgPhone 800 24971Fax 800 24984
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Phone +86 21 62351005Fax +86 21 [email protected]
FranceMiltenyi Biotec SAS10 rue Mercoeur75011 Paris, FrancePhone +33 1 56 98 16 16Fax +33 1 56 98 16 [email protected]
ItalyMiltenyi Biotec S.r.l.Via Persicetana, 2/D40012 Calderara di Reno (BO)ItalyPhone +39 051 6 460 411Fax +39 051 6 460 499
JapanMiltenyi Biotec K.K.Nittsu-Eitai Building 5F16-10 Fuyuki, Koto-ku, Tokyo135-0041, JapanPhone +81 3 5646 8910Fax +81 3 5646 8911
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SpainMiltenyi Biotec S.L.C/Luis Buuel 2Ciudad de la Imagen28223 Pozuelo de Alarcn(Madrid), SpainPhone +34 91 512 12 90
Fax +34 91 512 12 [email protected]
United KingdomMiltenyi Biotec Ltd.Almac House, Church LaneBisleySurrey GU24 9DR, UKPhone +44 1483 799 800Fax +44 1483 799 [email protected]
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