MACS Technology Flyer

8/6/2019 MACS Technology Flyer

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Consistent, high-quality separations

Easy handling

Compatible with downstream applications

Innovative products

From lab bench to clinical research applications

MACS TechnologyGold standard in cell separation


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CliniMACS Plus InstrumentThe CliniMACS Systema closed and sterilesystem for separation of large cell samples

From lab bench to clinical research applications

From small samples to samples ranging up to 1011 cells by

using columns with dierent cell loading capacity and their

suitable separators

Automated cell separation: The autoMACS Pro Separator

allows standardization o requent cell separations.

Sterile separations in a closed system: The CliniMACSPlus

Instrument in combination with GMP reagents opens new

opportunities or clinical research.

CD133+ cellsisolated withMACS Technology becameadherent and CD133during cultivation butgave rise to non-adherentCD133+ cells buddingrom the adherent cellsurace by asymmetriccell division

Compatible with downstream applications

Gentle on cells: small MicroBeads in combination

with MACS Column Technology allow the purication

o viable and unctionally active cells.

No inuence on analysis: small MicroBeads do not inuence

ow cytometric or microscopic analysis.

Suitable or cell culture and in vivo experiments: MicroBeads

are biodegradable, the short procedure and gentle

column technology avoid mechanical stress.

Molecular biology experiments: limited amount o small

MicroBeads avoid any inuence on urther experiments.

Innovative products

Continuous product development with ocus on current

research and clinical research needs

T cells: isolation o regulatory T cells, naive and memory/eectorcells, Th2 cells, or even antigen-specic T cells according to their

secretion o cytokines

Stem cells: CD133the marker or isolation o hematopoietic

and nonhematopoietic stem cells

Dendritic cells: unique kits or isolation o myeloid and

plasmacytoid human dendritic cells and mouse dendritic

cell subsets

Spleen cells beore separation Isolated CD4+CD25+ T cells

Plasmacytoid blooddendritic cellsisolated with MACSTechnologyandstained

with May-Grnwald-Giemsa

CD4+CD25+ regulatory T cells isolated rom mouse spleen usingMACS Technology

autoMACS Pro SeparatorWalk-away cell sorting of multiple samples


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Consistent, high quality separations

Optimal purity and recovery due to highly specic reagents

combined with efcient MACS Column Technology

Frequent cells such as T, B, NK cells and monocytes,

but also rare cells like stem cells, antigen-specic T cells,

and dendritic cell subsets

For any cell type: over 200 reagents or the direct isolation

o human, mouse, rat, and non-human primate cells;

any other cell type can be isolated using indirect labeling

with MicroBeads

Reliable and consistent results: based on extensive

experience in cell separation systems and rigorous

quality control

Easy handling

Convenient procedure:

easy-to-use system, applicable in every lab

Fast results: cells can be puried in up to 30 minutes

or positive selection or depletion due to short incubation

time and efcient MACS Column Technology;

no time-consuming bead detachment necessary

For positive selection and depletion strategies:

both labeled and unlabeled ractions can be obtained

with excellent purity and recovery

Prepackaged starting kits available:

consisting o a magnet and stand, columns and reagents

CD4+ T cells isolated using an LSColumn and a MidiMACS Separator

CD4+ T cells isolated usingthe autoMACS Separator

Bone marrow cellsbeore separation Isolated CD34+ cells

Mouse spleen

Human bone marrowMiniMACS SeparatorBasic tool for the lab:MiniMACS Separator with an MS Column

QuadroMACS SeparatorFor the simultaneous separation of 4 samples

The advantages


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Magnetic separation

MACS Column Technologyprovides a high-gradient

magnetic ield.

Gentletocells

Thoroughrinsing

procedure

Highrecovery

Untouched isolation by

depletion o speciic cells

Magnetic labeling

Gentle to cells;

minimal inluence

on downstream

experiments

Elution of the labeled cell fraction

Optimal resultseven or rare cells

by using positive selection

N S

Electron micrographs on this page with courtesy o Pro. Peter Groscurth, Institute o Anatomy, University o Zrich, Switzerland.


MACS Technology

MACS Technology has become the standard

method or cell separation. Numerous

publications have proven its versatility ormultiple applications: cell separations with

consistent high-quality results rom lab bench

to clinical applications, rom small to large

scale, rom requently occurring cells to rare

cells and sophisticated subsets. Miltenyi Biotec

provides researchers worldwide with the tools

or high-quality separations.

The principle

MACS Technology is based on MACS

MicroBeads, MACS Separators, and

MACS Columns. MACS MicroBeads aresuperparamagnetic particles o approximately

50 nanometers in diameter. They are

composed o a biodegradable matrix, and it is

thereore not necessary to remove them rom

cells ater the separation process.

Usually, MACS MicroBeads do not alter

structure, unction, or activity status o labeled

cells and are not known to interere with

subsequent experiments.

MACS Technology takes place within MACS

Columns. When a MACS Column is placed in a

MACS Separator, a strong permanent magnet,

a high-gradient magnetic eld is induced on

the column matrixstrong enough to retain

cells labeled with minimal amounts o MACS

MicroBeads. Unlabeled cells pass through and

can be collected; labeled cells are released

ater removal o the column rom the magnet.

Thus, with MACS Technology both labeled and

unlabeled cell ractions can easily be isolated

with high purity. The entire procedure o

positive selection or depletion takes less than

30 minutes, and cells can immediately be used

or urther experiments.

MACS Cell Separation is based on the use o MACS

MicroBeads, MACS Columns, and MACS Separators.

Cells can easily be purifed or depleted in less than30 minutes.

Anti-Biotin-PE

CD4-FITC

CD34-FITC

CD1

33/2-PE


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Direct labeling is the astest way o magnetic labeling. It

requires only one labeling step since the specic antibody

is directly coupled to the magnetic particle. Direct labelingreduces the number o washing steps and, thereore, avoids

unnecessary cell loss. Miltenyi Biotec provides highly specic

monoclonal antibodies or low background and optimal

separation, targeted against surace markers o cells rom

various species such as human, mouse, rat, and non-human

primates. Fluorochrome-conjugated MACS Antibodies can be

added or the purpose o analyzing magnetically separated cell

ractions by ow cytometry or uorescence microscopy.

Direct magnetic cell labeling Indirect magnetic cell labeling

Indirect labeling is perormed i no direct MicroBeads or

the cell type o interest are available. Almost any monoclonal

or polyclonal antibody targeting any cell type rom anyspecies can be used or indirect labeling. Cells are labeled

with a primary antibody that is unconjugated, biotinylated, or

uorochrome-conjugated. In a second step, magnetic labeling

is perormed by using Anti-Immunoglobulin, Anti-Biotin,

Streptavidin, or Anti-Fluorochrome MicroBeads, respectively.

A cocktail o antibodies can also be used to isolate or deplete a

number o cell types concurrently.

Since indirect labeling amplies the magnetic label, it may be

the method o choice or magnetic separation according to

dimly expressed antigens.

MACS Magnetic Labeling

Antibody MicroBead

Streptavidin MicroBead

Biotinylated antibody

Fluorochrome-conjugated antibody

Anti-Immunoglobulin MicroBead

Streptavidin MicroBead

Anti-Fluorochrome MicroBead

Anti-Biotin MicroBead


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Positive selection strategy Untouched isolation

Positive selection means that the desired target cells are

magnetically labeled and isolated as the magnetically retained

cell raction.

A positive selection strategy should be considered or:

excellent purity, especially or rare cell enrichment,

excellent recovery, and

ast results.

Positive selection is the most direct and specic way to isolate

the target cells rom a heterogenous cell suspension. Binding

o antibodies (which are part o the MicroBeads) to the cell

surace does not aect viability or unction o the cells. Both

ractions, labeled and unlabeled, can be recovered and used.

Due to their composition o iron oxide and polysaccharide,

MicroBeads are biodegradable and typically disappear ater a

ew days in culture.

MACS Separation Strategies

Untouched isolation is perormed by depletion o undesired

cells. Non-target cells are magnetically labeled and eliminated

rom the cell mixture. The non-magnetic, untouched cell ractioncontains the target cells.

Untouched isolation should be considered:

or removal o unwanted cells,

i no specic antibody is available or target cells,

i binding o the antibody to the target cells is not desired, or

or subsequent isolation o a cell subset by means o

positive selection.

For many dierent cell types Miltenyi Biotec oers optimized

MACS Cell Isolation Kits containing pre-titrated cocktails o

antibodies directed against non-target cells.

Magnetic separationUndesired cells are retained in a

MACS Column placed in a MACS

Separator.

The target cells pass through the

column and are collected as the

enriched, unlabeled cell raction,

depleted o non-target cells.

Magnetic labeling

Non-target cells are magnetically

labeled with a biotinylated antibody

cocktail and Anti-Biotin MicroBeads.

Magnetic separationCells are separated in a

MACS Column placed in a

MACS Separator.

The low-through raction can

be collected as negative raction

depleted o the labeled cells.

Magnetic labeling

Cells o interest are magnetically

labeled with MACS MicroBeads.

Elution of the labeled cell fraction

The column is removed rom the

separator. The retained cells are

eluted as the enriched, positively

selected cell raction.


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Sequential sorting (1):Depletion followed by positive selection

Sequential sorting (2):MultiSort strategy

Cell subsets can be isolated by rst depleting the non-target

cells and then positively selecting the cell subsets o interest.

This strategy is useul i undesired cells in the cell suspensionexpress the same antigen which is used or positive selection o

the target cells. For isolation o extremely rare cells, it also can

be useul rst to deplete non-target cells rom the suspension.

Positive selection can then be carried out with the pre-enriched

raction to obtain very pure cells.

With MACS MultiSort Kits high numbers o cells, characterized

by multiple cell surace markers, can be sorted easily. Even rare

cells can be enriched very efciently.Multi-parameter sorting with MACS MultiSort Kits allows

sequential positive selections o cells. The target cells are

rst labeled with MACS MultiSort MicroBeads and positively

selected or the rst parameter. Then the cells are incubated

with the MultiSort Release Reagent which enzymatically

removes the MicroBeads rom the antibodies. In the next

step, the target cells are magnetically labeled with MACS

MicroBeads directed against a subset marker to be positively

selected a second time.

MACS Separation Strategies

1st magnetic labeling

Non-target cells are

magnetically labeled with a

biotinylated antibody

cocktail and Anti-Biotin

MicroBeads.

1st magnetic separation

Undesired cells are

retained in a MACS

Column placed in a

MACS Separator while

the unlabeled cells

pass through.

2nd magnetic labeling

Target cells are magnetically

labeled with MicroBeads

according to a subset marker.

2nd magnetic separation

Target cells are

retained in the column

while unlabeled cellspass through.

Ater the column is removed

rom the separator, the

target cells are eluted as the

enriched, positively selected

cell raction.

1st magnetic labeling

Cells o interest are

magnetically labeled with

MultiSort MicroBeads.

1st magnetic separation

Target cells are magnetically

isolated by positive

selection.

Release of magnetic

particles

MultiSort MicroBeads are

enzymatically released.

2nd magnetic labeling

Cell subset o interest is

labeled with MACS

MicroBeads according to asecond marker.

2nd magnetic separation

Target cells are positively

selected a second time.


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Miltenyi Biotec provides products and services worldwide. Visit www.miltenyibiotec.com/local to nd your nearest Miltenyi Biotec contact.

The CliniMACS System components: Instruments, Reagents, Tubing Sets, and PBS/EDTA Buer are manuactured and controlled under an ISO 13485 certied quality system. In Europe, the CliniMACS Systemcomponents areavailable as CE-marked medical devices. In the USA, the CliniMACS System components including t he CliniMACS Reagents are available or use only un der an approved Investigational New Drug(IND) application or Investigational Device Exemption (IDE). CliniMACS MicroBeads are or research use on ly and not or use in humans. autoMACS and CliniMACS are registered trademarks o Miltenyi Biotec

GmbH. MiniMACS and QuadroMACS are trademarks o Miltenyi Biotec GmbH. Unless otherwise specically indicated, Miltenyi Biotec products and services are or research use only and not or therapeuticor diagnostic use. Copyright 2010 Miltenyi Biotec GmbH. All rights reserved.

130-091-618.0

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Miltenyi Biotec oers a wide variety o products or magnetic

cell isolation in basic research as well as or clinical-grade cellseparationsproviding the perect tools or translational

research. The continuously growing portolio also includes

instrumentation and numerous antibodies or ow cytometry,

as well as products or sample preparation, cell activation and

expansion, and molecular analysis.

To nd out more, visit our homepage at

www.miltenyibiotec.com

Germany/Austria/SwitzerlandMiltenyi Biotec GmbHFriedrich-Ebert-Strae6851429 Bergisch GladbachGermanyPhone +49 2204 8306-0

Fax +49 2204 [email protected]

USA/CanadaMiltenyi Biotec Inc.2303 Lindbergh StreetAuburn, CA 95602, USAPhone 800 FOR MACSPhone +1 530 888 8871Fax +1 530 888 [email protected]

www.miltenyibiotec.com

AustraliaMiltenyi BiotecAustralia Pty. Ltd.Unit 16A, 2 Eden Park DriveNorth Ryde, NSW 2113AustraliaPhone +61 2 8877 7400

Fax +61 2 9889 [email protected]

BeneluxMiltenyi Biotec B.V.Postbus 85183, 3508 AD UtrechtThe [email protected]

Customer service NetherlandsPhone 0800 4020120Fax 0800 4020100Customer service BelgiumPhone 0800 94016Fax 0800 99626Customer service LuxembourgPhone 800 24971Fax 800 24984

ChinaMiltenyi Biotec Trading(Shanghai) Co., Ltd.Shanghai OfceFareast International Plaza ARm. 2301, No. 319 Xianxia Rd.Shanghai 200051, P.R. China

Phone +86 21 62351005Fax +86 21 [email protected]

FranceMiltenyi Biotec SAS10 rue Mercoeur75011 Paris, FrancePhone +33 1 56 98 16 16Fax +33 1 56 98 16 [email protected]

ItalyMiltenyi Biotec S.r.l.Via Persicetana, 2/D40012 Calderara di Reno (BO)ItalyPhone +39 051 6 460 411Fax +39 051 6 460 499

[email protected]

JapanMiltenyi Biotec K.K.Nittsu-Eitai Building 5F16-10 Fuyuki, Koto-ku, Tokyo135-0041, JapanPhone +81 3 5646 8910Fax +81 3 5646 8911

[email protected]

SingaporeMiltenyi BiotecAsia Pacic Pte Ltd.100 Beach Road#28-06 to 28-08 Shaw TowerSingapore 189702Phone +65 6238 8183Fax +65 6238 [email protected]

SpainMiltenyi Biotec S.L.C/Luis Buuel 2Ciudad de la Imagen28223 Pozuelo de Alarcn(Madrid), SpainPhone +34 91 512 12 90

Fax +34 91 512 12 [email protected]

United KingdomMiltenyi Biotec Ltd.Almac House, Church LaneBisleySurrey GU24 9DR, UKPhone +44 1483 799 800Fax +44 1483 799 [email protected]

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