HPTLC is a well known and versati le separation method which shows a lot of advantages and options in comparison to other separation techniques.

The method is fast and inexpensive and does not need time consuming pretreatments.

The basic dif ference between conventional TLC and HPTLC is only in Par ticle and Pore size of the Sorbents.

It is very useful in quantitative and qualitative analysis of pharmaceuticals.

The new method can be used in cl inical diagnostic systems easily; E.g. for blood and urine investigations.

The Principle of separation is adsorption. One or more compounds are spotted on a thin layer of adsorbent coated on a chromatographic plate. The mobile phase (developing solvent) flows through because of capillary action (against gravitational force). The compounds move according their affinities towards the adsorbent. The component with more affinity towards the stationary phase travels slower. The component with lesser affinity towards the stationary phase travels faster.

The glass plates to be coated are conveyed underneath a hopper f i l led with the adsorbent suspension.

The layer thickness is determined by either a f ixed gate with pre-set spacers or by a gate with adjustable distance.

The plates are moved by a motorized conveying system at a uniform feeding rate of 10 cm/s, to ensure a uniform layer.

The Automatic Plate Coater is available normally with a f ixed gate for pre-set layers of 300 and 500 microns, an adjustable gate for layer thicknesses 0–2mm.

The manual plate coater functions in the same manner as the automatic coater, except with this model the plates are pushed through by hand, one af ter the other and l i f ted of f on the other side.

The TLC Plate Coater is also with a f ixed gate for pre-set layers of 300 and 500 microns and an adjustable gate for layer thicknesses 0–2 mm

•The Drying Rack consists of ten individual aluminum trays, 20 x 20 cm, which can be stacked quickly and conveniently.

•A tin box for storing the trays and two wire handles, to move the stack while hot, are supplied.• •The Drying Rack is convenient to use, particularly when TLC plates are prepared with the automatic plate coater in large runs.

•The Drying Rack also comes in handy for plates smaller than 20 x 20 cm.

Used to cut HPTLC plates easily and more precisely

Cuts plates with a thickness up to 3 mm

Does not damage the sensit ive layer

Easy to handle. Read the required size from the scale directly

Helps saving costs on pre coated plates of high quality by preventing off cuts.

For proper execution of the dipping technique, the

chromatogram must be immersed and withdrawn

at a control led uniform speed.

Key features: Uniform ver tical speed, freely selectable between

30 mm/s and 50 mm/s.

Immersion time selectable between 1 and 8 seconds and indefinitely

The device can be set to accommodate 10 cm and 20 cm plate height

Battery operated, independent of power supply.

The TLC Plate Heater is designed for heating TLC plates to a given temperature, while ensuring homogenous heating across the plate.

The TLC Plate Heater has a CERAN® heating sur face which is resistant to al l common reagents and is easily cleaned.

The 20 x 20 cm heating sur face has a grid to faci l i tate correct posit ioning of the TLC plate.

Programmed and actual temperature are digital ly displayed.

The temperature is selectable between 25 and 200 °C. The plate heater is protected from overheating.

The Nanomat serves for easy application of samples in the form of spots onto TLC and HPTLC layers, precisely posit ioned and without damage to the layer.

The actual sample dosage is per formed with Disposable Capillary Pipettes, which are precisely guided by the Capil lary Holder.

The Nanomat is suitable for Conventional TLC plates including self-coated

plates up to 20 x 20 cm HPTLC plates 10 x 10 cm and 20 x 10 cm TLC and HPTLC sheets up to 20 x 20 cm

The instrument is suitable for routine use for medium sample throughput. In contrast to the Automatic TLC Sampler , changing the sample for the Linomat requires presence of an operator.

With the Linomat samples are sprayed onto the chromatographic layer in the form of narrow bands.

This technique allows larger volumes to be applied than by contact transfer (spotting).

During the spraying the solvent of the sample evaporates almost entirely concentrating the sample into a narrow band of selectable length.

Star ting zones sprayed on as narrow bands ensure the highest resolution attainable with any given Thin-Layer Chromatographic system.

Automatic sample application is a key factor for productiv ity of the TLC laboratory.

The requirements for an instrument serving

this purpose, i .e. precision, robustness during routine use and convenient handl ing are ful ly met by the Automatic TLC Sampler .

The ATS of fers ful ly automatic sample appl ication for qual itative and quantitative analyses as wel l as for preparative separations. I t is suited for routine use and high sample throughput in mass analysis.

Samples are either appl ied as spots through contact transfer (0.1–5 micro l iter) or as bands or rectangles (0.5 to > 50 micro l i ter) using the spray-on technique.

Star ting zones sprayed on as narrow bands of fer the best separation attainable with a given chromatographic system.

Appl ication in the form of rectangles al lows precise appl ication of large volumes without damaging the layer.

Prior to chromatography, these rectangles are focused into narrow bands with a solvent of high elution strength.

The ATS al lows »over spotting«, i .e. a sequential appl ication from dif ferent vials onto the same posit ion.

This technique can be used e.g. in pre-chromatographic derivatization.

The Automatic Developing Chamber of fers convenience, safety and reproducibil ity for developments of TLC/HPTLC plates and foils with the format up to 20 x 20 cm.

Chromatogram development is the most critical step of Thin-Layer Chromatography. In the Automatic Developing Chamber ADC this step is fully automatic and independent of environmental ef fects.

The activity and pre-conditioning of the layer, chamber saturation, developing distance and f inal drying can be pre-set and automatically monitored by the ADC .

Two modes of operation are possible: stand-alone with input of parameters via keypad, or remote operation from win CATS with process monitoring, documentation of operating parameters and repor ting.

A conventional Twin Trough Chamber is used for development. With such chambers existing analytical procedures can be used without change, while at the same time, environmental ef fects and operator errors are eliminated.

Principle: The HPTLC plate is placed into the chamber with the layer

facing down. The reservoir (3) is charged with developing solvent – the plate can be developed horizontally either from one side only or from opposite sides simultaneously, this way doubling the number of samples per plate. Chromatography is star ted when the glass strip (4) is brought into a ver tical position.

Unsaturated configuration: The conditioning tray (6) is empty; the glass plate (2) is

removed.

Saturated configuration: The conditioning tray (6) contains developing solvent; the

glass plate (2) is removed.

Pre-conditioning: The conditioning tray (6) contains conditioning l iquid; the

glass plate (2) is removed. Development is star ted af ter pre-conditioning is completed.

Sandwich configuration: The conditioning tray (6) is empty; the glass plate (2) is in

place..

Changing non-absorbing substances into detectable derivatives

Improving the detectability (lowering detection limits)

Detecting all sample components Selectively detecting certain substances Inducing fluorescence

Derivatization through the gas phase of fers rapid and uniform transfer of the reagent.

It is unfor tunate, that only few reagents are suitable. They include iodine, bromine and chlorine, as well as volatile acids, bases and some other gases l ike H2S, and NO.

In practice gas phase derivatization can be easily accomplished in twin trough chambers where the reagent is placed or generated (e.g. chlorine from HCl and permanganate) in the rear trough, while the plate facing the inside of the chamber is positioned in the front trough.

This al l glass reagent sprayer is a low cost yet ef f icient normally used in TLC alternative to the HPTLC Sprayer.

I t comes with a rubber pump but may also be operated from a compressed air or nitrogen supply.

The HPTLC Sprayer consists of a charger and a pump unit with two kinds of spray heads.

Spray head type A is for spray solutions of normal viscosity, e.g. lower alcohol solutions.

spray head type B is for l iquids of higher viscosity, e.g. sulfuric acid reagents.

For proper execution of the dipping technique, the

chromatogram must be immersed and withdrawn at a

controlled uniform speed.

By maintaining a wel l def ined ver tical speed and

immersion time, derivatization conditions can be

standardized and »t ide marks«, which can inter fere with

densitometric evaluation, are avoided .

Key features: Uniform ver tical speed, freely selectable between 30

mm/s and 50 mm/s.

Immersion time selectable between 1 and 8 seconds and indefinitely

The device can be set to accommodate 10 cm and 20 cm plate height

Battery operated, independent of power supply.

The scanner is designed to handle objects up to 200 x 200 mm.

As required, scanning can be per formed in reflectance or transmission mode, by absorbance or by f luorescence.

The scanner features three l ight sources, a deuterium lamp, a halogen-tungsten lamp and a high-pressure mercury lamp.

The scanning speed is selectable between 1 and 100 mm/s.

The spectral range is 190 to 800 nm. The entire spectral range can be used for spectra

recording; i f the emission range of one lamp is exceeded the scanner automatical ly switches to the next lamp.

Spectra recording is per formed with up to 100 nm/s at an oversampling rate of 40/nm.

The scanner is connected to a computer which controls al l act ions, processes data and generates the repor t.

Higher resolution of zones due to higher number of theoretical plates

Shor ter developing times Less solvent consumption Less background noise due to narrow size distribution of

par ticles Enormous flexibil ity Parallel separation of many samples with minimal t ime

requirement Unsurpassed clarity and simultaneous visual evaluation of

al l samples and sample components Simplif ied sample preparation due to single use of the

stationary phase Possibil ity of multiple evaluations of the plate with

dif ferent parameters because all fractions of the sample are stored

on the plate.