Hptlc presenation3

Click here to load reader

  • date post

  • Category


  • view

  • download


Embed Size (px)

Transcript of Hptlc presenation3

  • HPTLC is a well known and versatile separation method which shows a lot of advantages and options in comparison to other separation techniques.The method is fast and inexpensive and does not need time consuming pretreatments. The basic difference between conventional TLC and HPTLC is only in Particle and Pore size of the Sorbents.It is very useful in quantitative and qualitative analysis of pharmaceuticals.The new method can be used in clinical diagnostic systems easily; E.g. for blood and urine investigations.

  • The Principle of separation is adsorption. One or more compounds are spotted on a thin layer of adsorbent coated on a chromatographic plate. The mobile phase (developing solvent) flows through because of capillary action (against gravitational force). The compounds move according their affinities towards the adsorbent. The component with more affinity towards the stationary phase travels slower. The component with lesser affinity towards the stationary phase travels faster.

  • The glass plates to be coated are conveyed underneath a hopper filled with the adsorbent suspension.

    The layer thickness is determined by either a fixed gate with pre-set spacers or by a gate with adjustable distance.

    The plates are moved by a motorized conveying system at a uniform feeding rate of 10 cm/s, to ensure a uniform layer.

    The Automatic Plate Coater is available normally with a fixed gate for pre-set layers of 300 and 500 microns, an adjustable gate for layer thicknesses 02mm.

  • The manual plate coater functions in the same manner as the automatic coater, except with this model the plates are pushed through by hand, one after the other and lifted off on the other side.The TLC Plate Coater is also with a fixed gate for pre-set layers of 300 and 500 microns and an adjustable gate for layer thicknesses 02 mm

  • The Drying Rack consists of ten individual aluminum trays, 20 x 20 cm, which can be stacked quickly and conveniently.

    A tin box for storing the trays and two wire handles, to move the stack while hot, are supplied. The Drying Rack is convenient to use, particularly when TLC plates are prepared with the automatic plate coater in large runs.

    The Drying Rack also comes in handy for plates smaller than 20 x 20 cm.

  • Used to cut HPTLC plates easily and more precisely

    Cuts plates with a thickness up to 3 mm

    Does not damage the sensitive layer

    Easy to handle. Read the required size from the scale directly

    Helps saving costs on pre coated plates of high quality by preventing off cuts.

  • For proper execution of the dipping technique, the chromatogram must be immersed and withdrawn at a controlled uniform speed.

    Key features:Uniform vertical speed, freely selectable between 30 mm/s and 50 mm/s.

    Immersion time selectable between 1 and 8 seconds and indefinitely

    The device can be set to accommodate 10 cm and 20 cm plate height

    Battery operated, independent of power supply.

  • The TLC Plate Heater is designed for heating TLC plates to a given temperature, while ensuring homogenous heating across the plate.

    The TLC Plate Heater has a CERAN heating surface which is resistant to all common reagents and is easily cleaned.

    The 20 x 20 cm heating surface has a grid to facilitate correct positioning of the TLC plate.

    Programmed and actual temperature are digitally displayed.

    The temperature is selectable between 25 and 200 C. The plate heater is protected from overheating.

  • The Nanomat serves for easy application of samples in the form of spots onto TLC and HPTLC layers, precisely positioned and without damage to the layer. The actual sample dosage is performed with Disposable Capillary Pipettes, which are precisely guided by the Capillary Holder.

    The Nanomat is suitable forConventional TLC plates including self-coated plates up to 20 x 20 cmHPTLC plates 10 x 10 cm and 20 x 10 cmTLC and HPTLC sheets up to 20 x 20 cm

  • The instrument is suitable for routine use for medium sample throughput. In contrast to the Automatic TLC Sampler , changing the sample for the Linomat requires presence of an operator.

    With the Linomat samples are sprayed onto the chromatographic layer in the form of narrow bands.

    This technique allows larger volumes to be applied than by contact transfer (spotting).

    During the spraying the solvent of the sample evaporates almost entirely concentrating the sample into a narrow band of selectable length.

    Starting zones sprayed on as narrow bands ensure the highest resolution attainable with any given Thin-Layer Chromatographic system.

  • Automatic sample application is a key factor for productivity of the TLC laboratory. The requirements for an instrument serving this purpose, i.e. precision, robustness during routine use and convenient handling are fully met by the Automatic TLC Sampler .

    The ATS offers fully automatic sample application for qualitative and quantitative analyses as well as for preparative separations. It is suited for routine use and high sample throughput in mass analysis.

    Samples are either applied as spots through contact transfer (0.15 micro liter) or as bands or rectangles (0.5 to > 50 micro liter) using the spray-on technique.

  • Starting zones sprayed on as narrow bands offer the best separation attainable with a given chromatographic system. Application in the form of rectangles allows precise application of large volumes without damaging the layer. Prior to chromatography, these rectangles are focused into narrow bands with a solvent of high elution strength. The ATS allows over spotting, i.e. a sequential application from different vials onto the same position. This technique can be used e.g. in pre-chromatographic derivatization.

  • The Automatic Developing Chamber offers convenience, safety and reproducibility for developments of TLC/HPTLC plates and foils with the format up to 20 x 20 cm.

    Chromatogram development is the most critical step of Thin-Layer Chromatography. In the Automatic Developing Chamber ADC this step is fully automatic and independent of environmental effects.

    The activity and pre-conditioning of the layer, chamber saturation, developing distance and final drying can be pre-set and automatically monitored by the ADC .

    Two modes of operation are possible: stand-alone with input of parameters via keypad, or remote operation from win CATS with process monitoring, documentation of operating parameters and reporting.

    A conventional Twin Trough Chamber is used for development. With such chambers existing analytical procedures can be used without change, while at the same time, environmental effects and operator errors are eliminated.

  • Principle:The HPTLC plate is placed into the chamber with the layer facing down. The reservoir (3) is charged with developing solvent the plate can be developed horizontally either from one side only or from opposite sides simultaneously, this way doubling the number of samples per plate. Chromatography is started when the glass strip (4) is brought into a vertical position.

    Unsaturated configuration:The conditioning tray (6) is empty; the glass plate (2) is removed.

    Saturated configuration:The conditioning tray (6) contains developing solvent; the glass plate (2) is removed.

    Pre-conditioning:The conditioning tray (6) contains conditioning liquid; the glass plate (2) is removed. Development is started after pre-conditioning is completed.

    Sandwich configuration:The conditioning tray (6) is empty; the glass plate (2) is in place..

  • Changing non-absorbing substances into detectable derivativesImproving the detectability (lowering detection limits)Detecting all sample componentsSelectively detecting certain substancesInducing fluorescence

  • Derivatization through the gas phase offers rapid and uniform transfer of the reagent.

    It is unfortunate, that only few reagents are suitable. They include iodine, bromine and chlorine, as well as volatile acids, bases and some other gases like H2S, and NO.

    In practice gas phase derivatization can be easily accomplished in twin trough chambers where the reagent is placed or generated (e.g. chlorine from HCl and permanganate) in the rear trough, while the plate facing the inside of the chamber is positioned in the front trough.

  • This all glass reagent sprayer is a low cost yet efficient normally used in TLC alternative to the HPTLC Sprayer.

    It comes with a rubber pump but may also be operated from a compressed air or nitrogen supply.

    The HPTLC Sprayer consists of a charger and a pump unit with two kinds of spray heads.

    Spray head type A is for spray solutions of normal viscosity, e.g. lower alcohol solutions.

    spray head type B is for liquids of higher viscosity, e.g. sulfuric acid reagents.

  • For proper execution of the dipping technique, the chromatogram must be immersed and withdrawn at a controlled uniform speed. By maintaining a well defined vertical speed and immersion time, derivatization conditions can be standardized and tide marks, which can interfere with densitometric evaluation, are avoided .

    Key features:Uniform vertical speed, freely selectable between 30 mm/s and 50 mm/s.

    Immersion time selectable between 1 and 8 seconds and indefinitely

    The device can be set to accommodate 10 cm and 20 cm plate height

    Battery operated, independent of power supply.

  • The scanner is designed to handle objects up to 200 x 200 mm.As required, scanning can be performed in reflectance or transmission mode, by absorbance or by fluorescence.The scanner features three light sources, a deuterium lamp, a hal