Download - EVOS Light Cube selection guide EVOS Light Cube selection ... · EVOS® Light Cube selection guide EVOS® Light Cubes integrate LEDs and high-performance fi lters in a novel illumination

For Research Use Only. Not for use in diagnostic procedures. © Thermo Fisher Scientifi c Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientifi c and its subsidiaries unless otherwise specifi ed. Cy is a trademark of GE Healthcare. Magic Red is a trademark of ImmunoChemistry Technologies, LLC. Hilyte Fluor is a trademark of Anaspec, Inc. Thiolyte is a trademark of Calbiochem-Behring Corp. Fluoro-Jade is a trademark of Histo-Chem, Inc. DyLight is a trademark of Pierce Biotechnology, Inc. CO011553 0814

Find out more at lifetechnologies.com/evos

Light cube Dye Abs

peakEm

peakSpectral match

Cy®5

(628

/40

ex; 6

92/4

0 em

)

Alexa Fluor® 633 631 650 GoodAlexa Fluor® 635 634 647 FairAlexa Fluor® 647 650 670 GoodAlexa Fluor® 660 663 691 ExcellentAlexa Fluor® 680 679 704 GoodAPC (Allophycocyanin) 651 660 GoodBODIPY® 630/650-X 626 641 FairBODIPY® 650/665 647 665 GoodCarboxynaphthofl uorescein 600 674 ExcellentCellMask™ Deep Red plasma membrane stain 658 676 GoodCellRox® Deep Red 640 665 GoodClick-iT® Plus Alexa Fluor® 647 650 670 GoodCy®5 648 666 GoodDDAO 648 658 GoodDiD (Vybrant® DiD) 645 670 ExcellentDRAQ5® 650 680 ExcellentDyLight 633 638 658 GoodDyLight 649 654 673 GoodFluoSpheres® crimson microspheres 621 646 GoodFluoSpheres® dark red microspheres 657 683 GoodFxCycle™ Far Red 641 657 GoodHCS CellMask™ Deep Red stain 649 670 ExcellentHCS NuclearMask™ Deep Red 635 687 ExcellentLIVE/DEAD® Fixable Far Red 650 665 ExcellentLysoTracker® Deep Red 647 668 GoodMitoTracker® Deep Red FM 641 661 GoodNucRed® Dead 647 642 661 GoodNucRed® Live 647 638 686 ExcellentSYTO® 17 619 638 FairSYTO® 59 621 644 GoodSYTO® 60 649 681 ExcellentSYTO® 62 650 681 ExcellentSYTOX® Red 640 658 GoodTO-PRO®-3 642 657 GoodTOTO®-3 642 661 GoodVybrant® DyeCycle™ Ruby 638 686 Excellent

Cy®5.

5 (6

55/4

6 ex

; 794

/160

em

)

Alexa Fluor® 680 679 702 GoodAlexa Fluor® 700 696 719 GoodATTO® 635 635 658 FairATTO® 647 644 670 FairATTO® 655 663 683 FairATTO® 680 680 700 GoodATTO® 700 699 719 FairCy®5.5 672 690 GoodDY-675 675 699 GoodDyLight® 680 676 705 GoodHiLyte Fluor™ 680 688 702 GoodIRDye® 680 NHS Ester 683 710 FairIRDye® 700 Phosphoramidite 691 699 FairQdot® 705 Nanocrystals 300 702 FairQuasar® 705 688 706 FairSYTO® 60 650 681 Fair

Light cube Dye Abs

peakEm

peakSpectral match

Texa

s R

ed®

(585

/29

ex; 6

24/4

0 em

)

2-Dodecylresorufi n 582 595 Good5-ROX 577 603 Excellent7-AAD 549 644 GoodalamarBlue® 570 584 FairAlexa Fluor® 568 578 603 GoodAlexa Fluor® 594 590 618 ExcellentAlexa Fluor® 610 610 629 FairAmplex® Red 570 583 FairAmplex® Ultra Red 567 582 FairBOBO™-3 571 606 GoodBODIPY® 630/650-X 625 641 FairBODIPY® TR-X 588 621 ExcellentCalcium Crimson™ 589 608 ExcellentCellTrace™ BODIPY® TR methyl esther 597 625 ExcellentCellTrace™ calcein red-orange 575 592 GoodCellTracker™ Red CMTPX 84 613 ExcellentClick-iT® Alexa Fluor® 594 590 618 ExcellentCy®3.5 581 597 GoodDsRed2 563 588 FairDyLight® 594 593 618 ExcellentFluoSpheres® Crimson microspheres 620 646 GoodFluoSpheres® Red microspheres 577 608 ExcellentHCS CellMask™ Red cytoplasmic/nuclear 621 644 FairHCS CellMask™ Red stain 587 614 ExcellentHCS LipidTOX™ Red 582 615 ExcellentHCS NuclearMask™ Red 624 644 FairHcRed 594 649 FairKatushka (Turbo FP635) 588 635 ExcellentLIVE/DEAD® Fixable Red Dead Cell stain 595 613 ExcellentLOLO™-1 568 580 FairLysoTracker Red 573 592 GoodMagic Red® 570 630 GoodMitoTracker® Red CMXRos 578 597 ExcellentmCherry 587 615 ExcellentmKate 588 635 ExcellentmPlum™ 590 649 FairmRaspberry 598 625 GoodNile Red phospholipid 551 638 ExcellentpHrodo® Red 559 587 FairReAsH (TC-ReAsH™) 593 608 ExcellentResorufi n 570 583 FairRhodamine phalloidin 558 577 FairRhodamine Red-X 572 591 GoodSulforhodamine 101 577 593 GoodSYTO® 17 620 635 GoodSYTO® 59 622 645 FairSYTO® 61 619 649 FairSYTOX® AADvanced™ dead cell stain 546 647 GoodTexas Red® 592 615 ExcellentX-rhod-1 579 602 ExcellentYO-PRO®-3 612 628 GoodYOYO®-3 612 631 Good

EVOS® Light Cube selection guide

EVOS® Light Cubes integrate LEDs and high-performance fi lters in a novel illumination system that offers precise control, minimal maintenance, and exceptional reliability. Use this selection guide to choose the light cube that best matches your dye. Dyes indicated in bold have excellent spectral match.

For a complete list of light cubes, go to lifetechnologies.com/evoslightcubes

Light cube Dye Abs

peakEm

peakSpectral match

RFP

(531

/40

ex; 5

93/4

0 em

)

ActinRed™ 555 (rhodamine phalloidin) 540 565 ExcellentAlexa Fluor® 532 532 554 FairAlexa Fluor® 546 561 572 GoodAlexa Fluor® 555 555 565 ExcellentAmplex® Red 571 583 ExcellentAmplex® UltraRed 568 583 ExcellentBOBO™-3 570 602 GoodBODIPY® TMR 544 569 ExcellentCalcium Orange™ 549 575 ExcellentCarboxy SNARF®-1 548 587 ExcellentCellLights® RFP 552 585 ExcellentCellMask™ Orange 556 571 ExcellentCellROX® Orange 545 565 ExcellentCellTracker™ Orange 547 575 ExcellentCellTracker™ Red 585 612 GoodCy®3 552 570 ExcellentCy®3.5 579 597 GoodDiI (CellTracker™ DiI) 551 568 ExcellentDsRed Monomer 556 586 GoodDsRed2 563 582 FairDsRed-Express 557 579 GooddTomato 554 581 GoodDyLight® 594 562 576 GoodEthidium bromide 521 602 GoodEthidium homodimer-1 (EthD-1) 530 618 ExcellentFluoSpheres® Orange 539 560 GoodHCS LipidTOX™ Red 582 615 GoodHEX™ 533 558 ExcellentMitoTracker® Orange CMTMRos 551 576 ExcellentMitoTracker® Orange CM-H2TMRos 551 576 ExcellentmOrange 548 568 GoodmTangerine 568 585 GoodNile Red 551 636 GoodOFP 546 568 ExcellentpHrodo® Red 559 586 ExcellentPhycoerythrin (PE, R-phycoerythrin) 567 576 ExcellentPOPO™-3 532 573 ExcellentPO-PRO™-3 539 570 ExcellentPremo™ FUCCI Cell Cycle Sensor (G1 phase) 555 584 ExcellentPropidium iodide (PI) 530 618 GoodResorufi n 570 585 ExcellentRFP 552 585 ExcellentRhod™-2 551 576 ExcellentRhodamine Red 560 580 GoodROX™ (6-ROX) 568 595 GoodSpectrumOrange™ 559 588 ExcellentSYTOX® Orange 567 570 ExcellentTagRFP 555 584 ExcellentTAMRA™ 565 580 ExcellentTetramethylrhodamine (TRITC) 555 580 ExcellentTurboRFP 553 574 ExcellentVybrant® DyeCycle™ Orange 518 562 Excellent

Light cube Dye Abs

peakEm

peakSpectral match

GFP

(470

/22

ex; 5

10/4

2 em

)

LysoTracker® Green 504 511 GoodmHoneydew 478 561 ExcellentMitoTracker® Green FM 490 512 ExcellentNBD amine 450 530 ExcellentNBD-X 467 538 ExcellentNeurotrace® 500/525 Green 497 524 ExcellentNitrobenzoxadiazole 465 510 ExcellentNucGreen™ Dead Cell Stain 504 523 GoodOregon Green® 488 498 524 GoodOregon Green® 514 512 532 FairpHrodo® Green 509 533 GoodPremo™ FUCCI Cell Cycle Sensor (S/G2/M phases) 474 509 ExcellentQdot® 525 UV 525 FairQdot® 545 UV 543 FairQuant-iT™ PicoGreen® 502 522 GoodRhodamine 110 496 520 GoodRhodamine 123 507 529 FairRhodamine Green™ 497 523 GoodRhodol Green™ 496 523 GoodrsGFP (red-shifted GFP, S65T) 498 516 GoodS65C 479 507 GoodS65L 484 510 GoodS65T 488 511 GoodsgGFP™ (Super Glow GFP) 474 509 ExcellentSodium Green™ 506 532 GoodSpectrumGreen™ 498 522 GoodSurf Green EX 469 526 ExcellentSYBR® Gold 495 539 GoodSYBR® Green I 497 521 GoodSYBR® Safe 507 524 GoodSYTO® 11 506 525 FairSYTO® 13 487 505 GoodSYTO® 16 490 520 ExcellentSYTO® 45 452 484 GoodSYTO® 9 482 500 ExcellentSYTO® RNASelect™ 503 527 GoodSYTOX® Green 504 523 GoodTET™ 520 541 FairTO-PRO®-1 515 530 FairTOTO®-1 513 531 FairVybrant® DyeCycle™ Green 505 532 GoodwtGFP (wild type GFP, non-UV excitation) 475 509 ExcellentYFP 512 529 FairYO-PRO®-1 491 507 ExcellentYOYO®-1 490 509 Excellent


Light cube Dye Abs

peakEm

peakSpectral match

DAP

I (35

7/44

ex;

447

/60

em)

1,8-ANS 372 480 Excellent4-Methylumbelliferone 360 440 Excellent7-Amino-4-methylcoumarin (AMC) 351 430 Excellent7-Hydroxy-4-methylcoumarin 360 449 ExcellentABQ 344 445 ExcellentAcridine 362 462 ExcellentAlexa Fluor® 350 346 442 ExcellentAlexa Fluor® 405 401 421 FairAMCA 345 425 ExcellentAMCA-X 353 442 ExcellentBFP 382 448 ExcellentCellTrace™ Calcein Blue 373 440 ExcellentCellTrace™ Calcein Violet 401 451 GoodCalcofl uor White 350 440 ExcellentCascade Blue® 377 420 GoodDansyl 335 525 FairDAPI 345 525 ExcellentDiFMU (pH 9.0) 357 450 ExcellentDyLight® 350 353 432 ExcellentER-Tracker™ Blue-White DPX 371 554 FairFast Blue 360 440 ExcellentFluoSpheres® Blue 356 412 ExcellentHoechst 33258 345 487 ExcellentHoechst 33342 347 483 ExcellentLIVE/DEAD® Fixable Blue Dead Cell Stain 344 442 ExcellentLysoSensor™ Blue 374 424 ExcellentLysoTracker® Blue 373 422 ExcellentMarina Blue® 365 460 ExcellentMethoxycoumarin 360 410 GoodMonochlorobimane (mBCI) 380 461 GoodNucBlue® Fixed Cell Stain 345 455 ExcellentNucBlue® Live Cell Stain 347 483 ExcellentPacifi c Blue™ 405 455 ExcellentPBFI 360 420 GoodPhorwite® AR 360 430 GoodQuinine sulfate 349 461 ExcellentsgBFP™ (Super Glow BFP) 387 450 GoodSITS 365 460 GoodSpectrumBlue® 405 449 ExcellentThiofl avin TCN 350 460 ExcellentThiolyte® 378 483 GoodTrue Blue 365 425 ExcellentUvitex® SFC 365 435 ExcellentY66H 360 442 Excellent

Light cube Dye Abs

peakEm

peakSpectral match

GFP

(470

/22

ex; 5

10/4

2 em

)

5-Carboxyfl uorescein (5-FAM) 492 518 GoodAcridine orange + DNA 500 26 GoodAcridine yellow 470 550 ExcellentActinGreen™ 488 (Alexa Fluor® 488 phalloidin) 496 518 ExcellentAlexa Fluor® 430 432 540 GoodAlexa Fluor® 488 496 518 ExcellentATTO® 465 453 507 ExcellentAuramine 460 540 ExcellentAurophosphine 470 540 ExcellentBCECF 499 540 GoodBODIPY® 492/515 490 540 GoodBODIPY® 505/515 502 540 GoodBODIPY® FL 502 540 GoodBTC 464 540 ExcellentCalcein 494 540 GoodCalcium Green™-1 506 540 FairCatskill Green 540 482 540 ExcellentCellLights® GFP 488 507 ExcellentCellEvent™ Caspase-3/7 Green 488 507 ExcellentCellTracker™ Green 490 540 ExcellentCellROX® Green 485 520 ExcellentCFDA 494 540 GoodCFP 434 540 FairClick-iT® Plus EdU Alexa Fluor® 488 496 518 ExcellentCy®2 492 540 ExcellentCyQUANT® Direct (CyQUANT® GR) 500 540 ExcellentDAF-FM 493 540 GoodDiO (Vybrant® DiO) 489 506 ExcellentDiversa Green-FP 484 507 ExcellentDyLight® 488 493 518 ExcellentEmerald Green 490 540 GoodEvans Blue 460 515 GoodFITC (fl uorescein) 492 520 GoodFluo-3 506 540 GoodFluo-4 494 540 GoodFluoro-Emerald 494 518 GoodFluoro-Jade® B 480 525 ExcellentFluor-X 494 520 GoodFluoSpheres® Yellow-Green 501 513 GoodGFP (EGFP) 488 507 ExcellentH2DCFDA (H2-DCF, DCFR) 504 540 GoodHCS LipidTOX™ Green 498 506 FairHPTS (Solvent Green 7) 455 510 ExcellentLIVE/DEAD® Fixable Green 4 498 526 GoodLysoSensor™ Green 448 503 Excellent


Simply stunningSee beautiful science with EVOS® imaging systems and Molecular Probes® reagents.

CO011553 EVOS-Reference-Guide (Aug 2014)_v4.indd 1 09/09/2014 15:28

Transmitted light system

EVOS® XL Core Cell Imaging SystemCell culture and routine cell maintenance• Ergonomic design• Fits and operates inside cell culture hoods• Easy installation: no maintenance, assembly, alignment or calibration• All-in-one design: digital camera, precision optics, LCD screen and

USB storage

System highlightsHardware

Illumination LED for transmitted light

Contrast methods Transmitted light (bright field and phase contrast)

Objective turret 4-position (manual control)

Condenser working distance 60 mm

Stage Choice of fixed or mechanical stageMechanical stage has X/Y axis controls and vessel holder framework

LCD screen 12.1 in. high-resolution colour monitor with adjustable tilt

Camera High-sensitivity CMOS colour camera

Output ports 2 USB ports

Power supply AC adaptor

Dimensions Height: 553 mm (21.0 in.)Depth: 406 mm (16.0 in.)Width: 318 mm (12.5 in.)

Weight With fixed stage: 9.1 kg (20.1 lb)With mechanical stage: 10.0 kg (22.0 lb)

Transmitted light system

EVOS® XL Cell Imaging SystemColorimetric cell imaging and cell passaging• Easy installation: no maintenance, assembly, alignment or calibration• Five-position objective turret• Onboard software• All-in-one design: digital camera, precision optics, LCD screen and

USB storage


Illumination LED for transmitted light

Contrast methods Transmitted light (bright field and phase contrast)

Objective turret 5-position (front-mounted control)


Stage Mechanical “glide” stage with X/Y axis fine-positioning controlsInterchangeable vessel holders available

LCD screen 15 in. high-resolution colour monitor with adjustable tilt

Camera High-sensitivity interline CMOS colour camera

Output ports 3 USB ports, 1 DVI port (supports direct output to USB and networked storage)



Weight 15.3 kg (33.7 lb)

EVOS® FLoid® Cell Imaging StationRoutine fluorescence imaging and teaching• Simplicity—fully integrated system with intuitive, multilingual

user interface• Speed—quickly get results without warm-up, cool-down or

filter changes• Convenience—capture and print images on your bench rather

than in the darkroom• Information—find out about the most popular Molecular Probes®

reagents and protocols via the user interface• Robustness—no moving parts and long-life LEDs for reliable

day-to-day use

Epifluorescence solutions


Illumination Adjustable Intensity LED (50,000+ hour life)

Contrast methods Epifluorescence and transmitted light

Objective 20x fixed fluorite objective

Fluorescence channels DAPI (blue), FITC (green), and Texas Red® (red)

Working distance 5.9 mm

Stage Mechanical “glide” stage with fine range-of-motion control (4 mm movement in X/Y dimensions)Universal format, compatible with all vessel types

LCD screen 15 in. high-resolution colour monitor with adjustable tilt (1,366 x 768 pixels)

Camera Monochrome; high-sensitivity interline CCD camera

Output ports 4 USB ports (3 on side for accessories; 1 in front for data storage)



Weight 11.8 kg (26 lb)

EVOS® FL / EVOS® FL Colour Cell Imaging System Multicolour complex fluorescence imaging • Easy installation: no maintenance, assembly, alignment or calibration• Four-colour fluorescence and transmitted light applications• Five-position objective turret• Onboard software• All-in-one design: digital camera, precision optics, LCD screen and

USB storage



Illumination Adjustable intensity LED (>50,000-hour life per light cube)

Contrast methods Epifluorescence and transmitted light (bright field and phase contrast)

Objective turret 5-position

Fluorescence channels Simultaneously accommodates up to 4 fluorescent light cubes


Stage Mechanical “glide” stage with X-Y axis fine-positioning controlsInterchangeable vessel holders available

LCD screen 15 in. high-resolution colour monitor with adjustable tilt

Camera High-sensitivity interline CCD camera (choice of monochrome or colour)

Output ports 3 USB ports, 1 DVI port (supports direct output to USB and networked storage)





EVOS® FL Auto Cell Imaging SystemMultidimensional imaging, time-lapse stitching and Z-stack automation• Exceptional ease-of-use• Fully automated and motorised X/Y scanning stage, autofocus,

and flat-focus Z-stack• Advanced software featuring high-resolution mosaic tiling, multi-

position well scanning, object counting and time-lapse imaging• Outstanding versatility with a 5-position objective turret,

4-colour fluorescence, plus colour and black and white cameras• Touch-screen software control

EVOS® Onstage Incubator specifications

Compatible vessels Multiwell plates, 35 mm dishes, 60 mm petri dishes, T-25 flasks

Temperature range Ambient to 40ºC

C02 range 0-20%

02 range 0% to ambient

Humidity range >80% relative humidity at 37ºC

Dimensions Environment chamber:Height: 250 mm (9.8 in.) Depth:190 mm (7.5 in.) Width: 370 mm (14.6 in.)

Weight Environment chamber: 1.5 kg (3.3 lb) Control unit: 10 kg (22.0 lb)


Illumination Adjustable intensity LED (>50,000-hour life per light cube)

Contrast methods Epifluorescence and transmitted light (bright field and phase contrast)

Objective turret 5-position


Stage Automated X/Y scanning stage; interchangeable vessel holders available

LCD screen 22 in. high-resolution touch-screen colour monitor

Cameras Dual (monochrome and colour cameras)• Monochrome: high-sensitivity interline CCD camera• Colour: high-sensitivity CMOS; 1/2-inch 2,048 x 1,536,

3.1 megapixels

Captured images Monochrome camera:16-bit monochrome TIFF or PNG (12-bitdynamic range); 24-bit colour TIFF or PNG;JPEG, BMP (1,280 x 960 pixels); Video: WMV files

Colour camera:16-bit colour TIFF or PNG (12-bit dynamic range); 24-bit colour TIFF or PNG;JPEG, BMP (1,360 x 1,024 pixels)

Output ports Multiple USB ports, 1 display output with DVI adaptor (supports direct output to USB and networked storage)


Dimensions Height: 322 mm (12.7 in.) Depth: 472 mm (18.6 in.)Width: 343 mm (13.5 in.)


FLUORESCENCE

FLUORESCENCE

PHASE CONTRAST

PHASE CONTRAST

PHASE CONTRAST

OPTIONALONSTAGE

INCUBATOR

BRIGHT FIELD

BRIGHT FIELD

BRIGHT FIELDBRIGHT FIELD

DVI PORT OUTPUT

DVI PORT OUTPUT USB PORTSUSB PORTSUSB PORTSUSB PORTS SUPPORTS NETWORKED

STORAGE

SUPPORTS NETWORKED

STORAGE

SUPPORTS NETWORKED

STORAGE

MECHANICAL STAGE

MECHANICAL STAGE

MECHANICAL STAGE

MONOCHROMECAMERA

COLOURCAMERA

COLOURCAMERA

OPTIONAL MECHANICAL

STAGE

RELIEF PHASE MONOCHROME OR COLOUR

CAMERA

FLUORESCENCEPHASE CONTRAST

OPTIONALONSTAGE

INCUBATOR

BRIGHT FIELD DVI PORT OUTPUT

USB PORTS SUPPORTS NETWORKED

STORAGE

MONOCHROME AND COLOUR

CAMERAS

FULLYAUTOMATED

Control unit:Height: 370 mm (14.6 in.)Depth: 160 mm (6.3 in.)Width: 200 mm (7.9 in.)

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