User Bulletin Directions for the Use of the Sheath/Ethanol Filter
12/2018
Overview
These directions are for the use of the sheath/ethanol filter, part number 661744, for the following instruments: BD FACSAria™ II, BD FACSAria™ IIu, BD FACSAria™ III, BD FACSAria™ IIIu, Special Order Research Product (SORP) BD FACSAria™, SORP BD FACSAria™ IIu, BD FACSAria™ Fusion, SORP BD FACSAria™ Fusion, or a BD FACSAria™ system with a fluidics upgrade. This filter can be also found in the Convert Kit Sheath/EtOH Filter, part number 662915.
The sheath or ethanol shutdown filter can be used to filter either sheath fluid or ethanol shutdown fluid. You need to change the filter depending on the procedure you are performing and the indications in the software interface.
About this task
NOTE Before it is installed, the filter can be used as a sheath filter, or an ethanol filter. After the filter is installed for one of these purposes, you cannot use it for a different purpose. Because of this, you should label each filter so that you know which one to use for each purpose.
NOTE The same conversion tubing must be moved between the Sheath and Ethanol tank. Additional conversion tubing may be purchased so that each tank has its own designated tubing, part number 662915. When you use the filter as a sheath filter, you should change it every six months, or when increased debris in an FSC vs SSC plot indicates that the sheath filter needs to be replaced.
Warnings
1 DO NOT START INSTALLTION BEFORE RELEASING ALL THE PRESSURE FROM TANK. 2 DO NOT STRIKE THE FILTER AGAINST HARD SURFACES AS DAMAGE TO THE FILTER MAY RESULT. VISUALLY
INSPECT THE FILTER BODY FOR CRACKS BEFORE USE. 3 THE VENT CAP WILL COME OFF AFTER A ¼ OF A TURN. THE FILTER IS PRESSURIZED, SO AIM THE FILTER AWAY
FROM BODY OR INSTRUMENT AND INTO A SEPARATE CONTAINER BEFORE TURNING THE VENT CAP.
4 IF THE CONVERSION TUBING FILTER IS CONNECTED INCORRECTLY, IT MAY CAUSE DAMAGE TO THE
INSTRUMENT.
5 WEAR PROPER PERSONAL PROTECTION EQUIPMENT SUCH AS LAB COATS, SAFETY GLASSES OR SAFETY FACE
GUARD, AND GLOVES BEFORE HANDLING OF FILTERS.
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Sheath/Ethanol Filter User Bulletin
Filter Replacement OR Installation of the Conversion Tubing
1 Turn off the stream. 2 Disconnect the air line from the sheath tank. 3 Pull up on the ring of the pressure relief valve to release pressure from the tank. Verify that all of the pressure is
released by pulling it up a second time. 4 Disconnect the filter by pressing on the metal tabs on each end. 5 If converting, connect the conversion tubing to the tank using the “From Tank” end.
Figure 1: “From Tank” label on a conversion tubing connecting to the tank
6 Write the current date and the fluid type on the filter so that you know when to replace it.
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Sheath/Ethanol Filter User Bulletin
7 Use the arrows on the filter that indicate the direction of the flow through the filter and verify the flow direction
before inserting the line. Ensure that both vent caps are tightened before installing.
8 To connect the filter, press down on the quick disconnect metal tab on the filter until the pin pops up.
Figure 2: Pin and quick disconnect metal tab on the filter
9 Insert the conversion tubing end labeled “To Filter” and push in until you hear a Click.
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Sheath/Ethanol Filter User Bulletin
Figure 3: “To Filter” conversion tubing connection to Filter
10 Connect the supply line to the filter by repeating steps 8 and 9. Figure 4: Supply line connected to the other end of Filter
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Sheath/Ethanol Filter User Bulletin
11 Reconnect the air line to the tank and check for leaks when the pressure is turned on. Gently tap the filter to loosen air bubbles. DO NOT STRIKE THE FILTER AGAINST HARD SURFACES AS DAMAGE TO THE FILTER MAY RESULT. VISUALLY INSPECT FOR CRACKS BEFORE USE.
Figure 5: Air Line connected to the tank
12 Place a small container under the vent cap to catch any fluid.
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Sheath/Ethanol Filter User Bulletin
13 Cover the vent cap with a paper towel or a lab wipe and slowly loosen the vent cap to release the air. Aim the
filter away from body or instrument and into a separate container. The paper towel or wipe will prevent the
cap from popping off too quickly. If the cap is loosened too quickly, the cap will come off before any air is
released.
Figure 6: Opening vent cap with paper towel or lab wipe
WARNING: CAP WILL COME OFF AFTER A ¼ OF A TURN. THE FILTER IS PRESSURIZED, SO AIM THE FILTER AWAY
FROM BODY OR INSTRUMENT AND INTO A SEPARATE CONTAINER.
14 Close the vent cap. 15 Wipe up any excess fluid that might have dripped outside the container.
Purging the Fluid Filters
1. Place a small container under the vent cap to catch any fluid.
2. Cover the vent cap with a paper towel or a lab wipe and slowly loosen the vent cap to release the air. Aim
the filter away from body or instrument and into a separate container. The paper towel or wipe will
prevent the cap from popping off too quickly. If the cap is loosened too quickly, the cap will come off
before any air is released.
3. Close the vent cap.
Vent Cap
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Sheath/Ethanol Filter User Bulletin
4. Wipe up any excess fluid that might have dripped outside the container.
NOTE:
When switching the lines between the ethanol and sheath tank, verify that the lines are connected correctly
before attaching the air line. Incorrect connections may cause damage to the instrument.
Figure 7: Correct connection between the lines on Sheath Tank
Sheath tank (use a filter that is
dedicated to sheath):
1) “To Filter” end of
conversion tubing
connected to filter
2) “To Tank” end of
conversion tubing
connected to tank
3) Air line (clear tubing)
connected to inlet on
tank
4) Supply line connected to
filter
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Sheath/Ethanol Filter User Bulletin
Figure 8: Correct connection between the lines on Ethanol Tank
Ethanol Tank (Use a filter that is dedicated to
ethanol): Same connection as the sheath tank
1) “To Tank” end of conversion tubing
connected to the tank
2) “To Filter” end of conversion tubing
connected to filter
3) Air line (clear tubing) connected to inlet
on tank
4) Supply line connected to filter
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Sheath/Ethanol Filter User Bulletin
Figure 10: Example of incorrect connection of air line
Figure 11: Example of incorrect connection
DO NOT connect the air line to the “To
Filter” end of the conversion tubing.
DO NOT connect the two tanks with the
conversion tubing.
X
X
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Sheath/Ethanol Filter User Bulletin
Figure 12: Another example of incorrect connection
For customers who purchased an extra
conversion tubing, DO NOT connect the
two tanks.
X
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Sheath/Ethanol Filter User Bulletin
Copyrights © 2018, Becton, Dickinson and Company. All rights reserved. No part of this publication may be reproduced, transmitted, transcribed, stored in retrieval systems, or translated into any language or computer language, in any form or by any means: electronic, mechanical, magnetic, optical, chemical, manual, or otherwise, without prior written permission from BD Biosciences. The information in this guide is subject to change without notice. BD Biosciences reserves the right to change its products and services at any time to incorporate the latest technological developments. Although this guide has been prepared with every precaution to ensure accuracy, BD Biosciences assumes no liability for any errors or omissions, nor for any damages resulting from the application or use of this information. BD Biosciences welcomes customer input on corrections and suggestions for improvement. Trademarks © 2018 BD. BD, the BD Logo, and all other trademarks are property of Becton, Dickinson and Company. Regulatory information Class 1 Laser Product.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
23-21212-00
12/2018
Becton, Dickinson and Company BD Biosciences
2350 Qume Drive San Jose, CA 95131 USA bdbiosciences.com [email protected]
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Tel 32.2.400.98.95 Fax 32.2.401.70.94 [email protected]
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