Directions for the Use of the Sheath/Ethanol Filter · 2019-11-22 · 2 Sheath/Ethanol Filter User...

User Bulletin Directions for the Use of the Sheath/Ethanol Filter

12/2018

Overview

These directions are for the use of the sheath/ethanol filter, part number 661744, for the following instruments: BD FACSAria™ II, BD FACSAria™ IIu, BD FACSAria™ III, BD FACSAria™ IIIu, Special Order Research Product (SORP) BD FACSAria™, SORP BD FACSAria™ IIu, BD FACSAria™ Fusion, SORP BD FACSAria™ Fusion, or a BD FACSAria™ system with a fluidics upgrade. This filter can be also found in the Convert Kit Sheath/EtOH Filter, part number 662915.

The sheath or ethanol shutdown filter can be used to filter either sheath fluid or ethanol shutdown fluid. You need to change the filter depending on the procedure you are performing and the indications in the software interface.

About this task

NOTE Before it is installed, the filter can be used as a sheath filter, or an ethanol filter. After the filter is installed for one of these purposes, you cannot use it for a different purpose. Because of this, you should label each filter so that you know which one to use for each purpose.

NOTE The same conversion tubing must be moved between the Sheath and Ethanol tank. Additional conversion tubing may be purchased so that each tank has its own designated tubing, part number 662915. When you use the filter as a sheath filter, you should change it every six months, or when increased debris in an FSC vs SSC plot indicates that the sheath filter needs to be replaced.

Warnings

1 DO NOT START INSTALLTION BEFORE RELEASING ALL THE PRESSURE FROM TANK. 2 DO NOT STRIKE THE FILTER AGAINST HARD SURFACES AS DAMAGE TO THE FILTER MAY RESULT. VISUALLY

INSPECT THE FILTER BODY FOR CRACKS BEFORE USE. 3 THE VENT CAP WILL COME OFF AFTER A ¼ OF A TURN. THE FILTER IS PRESSURIZED, SO AIM THE FILTER AWAY

FROM BODY OR INSTRUMENT AND INTO A SEPARATE CONTAINER BEFORE TURNING THE VENT CAP.

4 IF THE CONVERSION TUBING FILTER IS CONNECTED INCORRECTLY, IT MAY CAUSE DAMAGE TO THE

INSTRUMENT.

5 WEAR PROPER PERSONAL PROTECTION EQUIPMENT SUCH AS LAB COATS, SAFETY GLASSES OR SAFETY FACE

GUARD, AND GLOVES BEFORE HANDLING OF FILTERS.

2

Sheath/Ethanol Filter User Bulletin

Filter Replacement OR Installation of the Conversion Tubing

1 Turn off the stream. 2 Disconnect the air line from the sheath tank. 3 Pull up on the ring of the pressure relief valve to release pressure from the tank. Verify that all of the pressure is

released by pulling it up a second time. 4 Disconnect the filter by pressing on the metal tabs on each end. 5 If converting, connect the conversion tubing to the tank using the “From Tank” end.

Figure 1: “From Tank” label on a conversion tubing connecting to the tank

6 Write the current date and the fluid type on the filter so that you know when to replace it.

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7 Use the arrows on the filter that indicate the direction of the flow through the filter and verify the flow direction

before inserting the line. Ensure that both vent caps are tightened before installing.

8 To connect the filter, press down on the quick disconnect metal tab on the filter until the pin pops up.

Figure 2: Pin and quick disconnect metal tab on the filter

9 Insert the conversion tubing end labeled “To Filter” and push in until you hear a Click.

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Figure 3: “To Filter” conversion tubing connection to Filter

10 Connect the supply line to the filter by repeating steps 8 and 9. Figure 4: Supply line connected to the other end of Filter

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11 Reconnect the air line to the tank and check for leaks when the pressure is turned on. Gently tap the filter to loosen air bubbles. DO NOT STRIKE THE FILTER AGAINST HARD SURFACES AS DAMAGE TO THE FILTER MAY RESULT. VISUALLY INSPECT FOR CRACKS BEFORE USE.

Figure 5: Air Line connected to the tank

12 Place a small container under the vent cap to catch any fluid.

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13 Cover the vent cap with a paper towel or a lab wipe and slowly loosen the vent cap to release the air. Aim the

filter away from body or instrument and into a separate container. The paper towel or wipe will prevent the

cap from popping off too quickly. If the cap is loosened too quickly, the cap will come off before any air is

released.

Figure 6: Opening vent cap with paper towel or lab wipe

WARNING: CAP WILL COME OFF AFTER A ¼ OF A TURN. THE FILTER IS PRESSURIZED, SO AIM THE FILTER AWAY

FROM BODY OR INSTRUMENT AND INTO A SEPARATE CONTAINER.

14 Close the vent cap. 15 Wipe up any excess fluid that might have dripped outside the container.

Purging the Fluid Filters

1. Place a small container under the vent cap to catch any fluid.

2. Cover the vent cap with a paper towel or a lab wipe and slowly loosen the vent cap to release the air. Aim

the filter away from body or instrument and into a separate container. The paper towel or wipe will

prevent the cap from popping off too quickly. If the cap is loosened too quickly, the cap will come off

before any air is released.

3. Close the vent cap.

Vent Cap

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4. Wipe up any excess fluid that might have dripped outside the container.

NOTE:

When switching the lines between the ethanol and sheath tank, verify that the lines are connected correctly

before attaching the air line. Incorrect connections may cause damage to the instrument.

Figure 7: Correct connection between the lines on Sheath Tank

Sheath tank (use a filter that is

dedicated to sheath):

1) “To Filter” end of

conversion tubing

connected to filter

2) “To Tank” end of

conversion tubing

connected to tank

3) Air line (clear tubing)

connected to inlet on

tank

4) Supply line connected to

filter

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Figure 8: Correct connection between the lines on Ethanol Tank

Ethanol Tank (Use a filter that is dedicated to

ethanol): Same connection as the sheath tank

1) “To Tank” end of conversion tubing

connected to the tank

2) “To Filter” end of conversion tubing

connected to filter

3) Air line (clear tubing) connected to inlet

on tank

4) Supply line connected to filter

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Figure 10: Example of incorrect connection of air line

Figure 11: Example of incorrect connection

DO NOT connect the air line to the “To

Filter” end of the conversion tubing.

DO NOT connect the two tanks with the

conversion tubing.

X

X

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Figure 12: Another example of incorrect connection

For customers who purchased an extra

conversion tubing, DO NOT connect the

two tanks.

X

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Copyrights © 2018, Becton, Dickinson and Company. All rights reserved. No part of this publication may be reproduced, transmitted, transcribed, stored in retrieval systems, or translated into any language or computer language, in any form or by any means: electronic, mechanical, magnetic, optical, chemical, manual, or otherwise, without prior written permission from BD Biosciences. The information in this guide is subject to change without notice. BD Biosciences reserves the right to change its products and services at any time to incorporate the latest technological developments. Although this guide has been prepared with every precaution to ensure accuracy, BD Biosciences assumes no liability for any errors or omissions, nor for any damages resulting from the application or use of this information. BD Biosciences welcomes customer input on corrections and suggestions for improvement. Trademarks © 2018 BD. BD, the BD Logo, and all other trademarks are property of Becton, Dickinson and Company. Regulatory information Class 1 Laser Product.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

23-21212-00

12/2018

Becton, Dickinson and Company BD Biosciences

2350 Qume Drive San Jose, CA 95131 USA bdbiosciences.com [email protected]

BD Biosciences European Customer Support

Tel 32.2.400.98.95 Fax 32.2.401.70.94 [email protected]

Directions for the Use of the Sheath/Ethanol Filter · 2019-11-22 · 2 Sheath/Ethanol Filter User...

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