Genalice B.V. the Netherlands confiden'al
NO INFINITE PROBABILITY DRIVE INSIDE UP TO 200X FASTER THAN CONVENTIONAL TOOLS
WE HAVE ALWAYS THOUGHT THERE WAS SOMETHING FUNDAMENTALLY WRONG WITH DNA MAPPING
THE END OF THE BIG BAM THEORY
GENALICE Building the “Google DNA” company
ICT & MedTech: new business opportuni'es
Jos Lunenberg Chief Business Officer
Today’s Journey
▲ the drive
▲ the opportunity
▲ the status
▲ the environment
▲ the route
▲ the hurdles
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THE DRIVE
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One Size Does Not Fit All
The drive
DNA 3.2 billion base pairs
Organized in 23 chromosome pairs
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The cause
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The soluIon
Targeted treatment based on molecular profiling is the solu'on
PaIents with same condiIon DNA Profiling PaIents with same condiIon
Good responders
No responders
Bad side effects
Personalized Medicine
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The soluIon
Biomarkers and the diagnosIcs process
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The soluIon
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THE OPPORTUNITY
At the brink of the BIGGEST change in medicine Enabled through advances in DNA digitaliza'on and by powerful
data analysis
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The vision
Adapted from “The Crea've Destruc'on of Medicine” by Eric Topol, MD
Cell Phone
Personal Computer
Internet
Digital Devices
Sequencing
Social Networks
The Great InflecIon of Medicine
Accelera.on of Technology
1970 1980 1990 2000 2010 2020
Main sequencing methods & cost development
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Company Method Sanger Ins'tute Sanger Roche 454 pyro Illumina Solexa Applied Biosystems (LT) SOLiD Life Technologies Ion Torrent semiconductor Complete Genomics DNA nanoball Helicos Biosciences Heliscope single molecule Pacific Biosciences Single molecule real 'me (SMRT) Oxford Nanopore Nanopore DNA
Illumina’s introduction of the HiSeq X Ten
The market developments
DNA data digitalizaIon is skyrockeIng Number of sequenced full human genomes is exploding
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The momentum
Adapted from Technology Review, May/June 2012, ‘Bases to Bytes’ by Mike Orcutt
Hans Karten (1961)
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The wizkid
Chief ExecuIve & Technology Officer
▲ BSc Electrical Engineering
▲ 14 years career at Oracle Corpora'on, patentee of 11 patents
▲ 7 years pro-‐deo “Chairman of the Bap'st Church” in Harderwijk
▲ 3 year technical due-‐diligences for a Venture Capitalist
▲ Co-‐founder of GENALICE
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The mission
Data processing and analysis soluIon for sequencing speed increases
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THE STATUS
DNA data processing & analysis workflow GENALICE product proposi'ons
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GENALICE MAP
GENALICE LINK
GENALICE CHECK
The product porcolio
Downstream analysis
Individual NGS data analyses
Clinical Decision Support
Preprocessing Next
GeneraIon Sequencing
Correlation with other domains
&
Next-‐GeneraIon Sequencing data processing Preprocessing components
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MAP in overview
Components Alignment Variant calling Annotation
Description Mapping individual reads on right position
Statistical correction of detected variants
Variant qualification and association with existing knowledge
Preprocessing
Up to 100x faster -‐ from FASTQ to VCF in 30 minutes* using a small commodity hardware server with a dual Intel Xeon E5 processor
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IntergalacIc speed
*NGS data preprocessing for one full human genome (37x coverage)
Sto
rage
redu
ctio
n (fo
ld c
hang
e)
Human1000 genomes
Cultivatedtomato
Wildtomato
0
25
50
75
100
100x
71x
15x
Taking control of the NGS big data deluge Up to 100x smaller alignment output file
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Size magers
400 GB
4 GB
CONVENTIONAL TOOLS:
GENALICE MAP:
ALIGNMENT OUTPUT PER HUMAN GENOME
GENALICE MAP product proposiIon Delivered in an all-‐in-‐one appliance: the GENALICE VAULT
Product components: ▲ MAP nodes (alignment & variant calling)
▲ CONTROL node (workflow management & real-‐'me monitoring)
▲ GAR toolkit (incl. SAM/BAM/FASTQ converter and IGV viewer)
Product features: ▲ Storage on board (incl. SSDs)
▲ Embedded Oracle database
▲ Graphical User Interface
▲ High level security
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Scalable soluIon
* HGE37 = Human Genome Equivalents with an average of 37x coverage sequenced # Each MAP server only contains a dual standard INTEL XEON Processor E5-2620
2 4
XS S M L XL Storage Maximum number of HGE37* 500 1,000 2,500 4,000 10,000
Performance align & call HGE37/day average 48 144 240 336 624
minutes to align & call a HGE37 average 30 10 6 4 2
Average total realignment days for fully loaded appliance 10 7 10 12 16
THE ENVIRONMENT
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CompeIIve environment MAP A crowded & scagered market space…
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▲ Open source tools
Software Hardware
Open Source
Commercial
Complementary
▲ Commercial specific hardware soluIons
▲ Solware soluIons/suites ▲ Consultancy services ▲ Turnkey appliances
▲ Solware suites included in NGS machines by its vendors
Cloud enabled
FPGA accelerated
▲ Cloud services ▲ Cloud placorm
▲ Descrip'on: Processing of raw NGS data in prepara'on of analysis
Market Environment
CompeIIve environment MAP …with many players
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Software Hardware
Open Source
Commercial
Complementary
Cloud enabled
FPGA accelerated
BowTie2, BWA
Market Environment
CompeIIve environment MAP …but also one with significant room for improvement
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Speed
Format (C/E) Accuracy
Market Environment
THE ROUTE
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Business Strategy
▲ Key / Social Opinion Leader management
▲ Focus on high volume centers/ini'a'ves
▲ Focus high complexity data opportuni'es
▲ Disrup've market penetra'on (costly)
▲ Partnerships with global top ins'tutes
▲ Partnerships with key market players (e.g. Intel, Illumina)
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THE PLAN
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Global Launch
42andMoore – seong new unofficial WR
THE HURDLES
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THE HURDLES
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▲ Team
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The team
THE HURDLES
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▲ Team
▲ Money
THE HURDLES
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▲ Team
▲ Money
▲ Market dynamics
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The market
THE HURDLES
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▲ Team
▲ Money
▲ Market dynamics
▲ Support
Excellent team and network Our board of directors
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Former posiIons: CEO of Crucell (sold to J&J for 2.4B) Board member Galapagos (listed AMEX)
Professor of entrepreneurship at IESE Business School in Barcelona Former posiIons: Group Management Royal Philips Chairman of the Board at Crucell
Serial Enterpreneur Former posiIons: Founder and Managing Director Tango and RouteMobiel
Excellent team and network Our scien'fic advisory board
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Prof. Edwin Cuppen UMC Utrecht
Head of Research Medical Gene'cs
Amsterdam
Prof. Gerrit Meijer VUmc Amsterdam
Principal Inves'gator TraIT (CTMM)
THE HURDLES
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▲ Team
▲ Money
▲ Market dynamics
▲ Support
▲ Credibility
Credibility
▲ Live up to claims & promises
▲ KOL support
▲ Give opportunity to test drive
▲ Push new format
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Low cost
High speed
High quality
the
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company
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