Genalice B.V. the Netherlands confiden'al  

NO  INFINITE  PROBABILITY  DRIVE  INSIDE   UP  TO  200X  FASTER  THAN  CONVENTIONAL  TOOLS  

WE  HAVE  ALWAYS  THOUGHT  THERE  WAS  SOMETHING  FUNDAMENTALLY  WRONG  WITH  DNA  MAPPING  

THE  END  OF  THE  BIG  BAM  THEORY  

GENALICE  Building  the  “Google  DNA”  company    

ICT  &  MedTech:    new  business  opportuni'es  

Jos  Lunenberg  Chief  Business  Officer  

Today’s  Journey  

▲  the  drive  

▲  the  opportunity  

▲  the  status  

▲  the  environment  

▲  the  route    

▲  the  hurdles  

 

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THE  DRIVE  

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One  Size  Does  Not  Fit  All  

The  drive  

DNA    3.2  billion  base  pairs  

Organized  in  23  chromosome  pairs  

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The  cause  

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The  soluIon  

Targeted  treatment    based  on  molecular  profiling  is  the  solu'on  

PaIents  with  same  condiIon  DNA  Profiling  PaIents  with  same  condiIon  

Good  responders  

No  responders  

Bad  side  effects  

Personalized  Medicine  

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The  soluIon  

Biomarkers  and  the  diagnosIcs  process  

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The  soluIon  

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THE  OPPORTUNITY  

At  the  brink  of  the  BIGGEST  change  in  medicine    Enabled  through  advances  in  DNA  digitaliza'on  and  by  powerful    

data  analysis  

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The  vision  

Adapted  from  “The  Crea've  Destruc'on  of  Medicine”  by  Eric  Topol,  MD    

Cell  Phone  

Personal  Computer  

Internet  

Digital  Devices  

Sequencing  

Social    Networks  

The  Great  InflecIon  of  Medicine  

Accelera.on  of  Technology  

                       

1970                              1980                          1990                    2000                                  2010                                  2020  

Main  sequencing  methods  &  cost  development  

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Company   Method  Sanger  Ins'tute   Sanger  Roche   454  pyro  Illumina   Solexa    Applied  Biosystems  (LT)   SOLiD    Life  Technologies   Ion  Torrent  semiconductor  Complete  Genomics   DNA  nanoball    Helicos  Biosciences   Heliscope  single  molecule  Pacific  Biosciences   Single  molecule  real  'me  (SMRT)  Oxford  Nanopore   Nanopore  DNA    

Illumina’s introduction of the HiSeq X Ten

The  market  developments  

DNA  data  digitalizaIon  is  skyrockeIng  Number  of  sequenced  full  human  genomes  is  exploding  

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The  momentum  

Adapted from Technology Review, May/June 2012, ‘Bases to Bytes’ by Mike Orcutt

Hans  Karten  (1961)  

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The  wizkid  

Chief  ExecuIve  &  Technology  Officer    

▲  BSc  Electrical  Engineering  

▲  14  years  career  at  Oracle  Corpora'on,  patentee  of  11  patents  

▲  7  years  pro-­‐deo  “Chairman  of  the  Bap'st  Church”  in  Harderwijk  

▲  3  year  technical  due-­‐diligences  for  a  Venture  Capitalist  

▲  Co-­‐founder  of  GENALICE  

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The  mission  

Data  processing  and  analysis  soluIon    for  sequencing  speed  increases  

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THE  STATUS  

DNA  data  processing  &  analysis  workflow  GENALICE  product  proposi'ons  

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GENALICE MAP

GENALICE LINK

GENALICE CHECK

The  product  porcolio  

Downstream  analysis  

Individual NGS data analyses

Clinical  Decision    Support  

Preprocessing  Next  

GeneraIon    Sequencing  

 

Correlation with other domains

&  

Next-­‐GeneraIon  Sequencing  data  processing    Preprocessing  components  

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MAP  in  overview  

Components Alignment Variant calling Annotation

Description Mapping individual reads on right position

Statistical correction of detected variants

Variant qualification and association with existing knowledge

Preprocessing  

Up  to  100x  faster  -­‐  from  FASTQ  to  VCF  in  30  minutes*  using  a  small  commodity  hardware  server  with  a  dual  Intel  Xeon  E5  processor  

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IntergalacIc  speed  

*NGS data preprocessing for one full human genome (37x coverage)

Sto

rage

redu

ctio

n (fo

ld c

hang

e)

Human1000 genomes

Cultivatedtomato

Wildtomato

0

25

50

75

100

100x

71x

15x

Taking  control  of  the  NGS  big  data  deluge    Up  to  100x  smaller  alignment  output  file  

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Size  magers  

400  GB  

4  GB  

CONVENTIONAL  TOOLS:  

GENALICE  MAP:  

ALIGNMENT OUTPUT PER HUMAN GENOME

GENALICE  MAP  product  proposiIon  Delivered  in  an  all-­‐in-­‐one  appliance:  the  GENALICE  VAULT  

Product  components:  ▲  MAP  nodes  (alignment  &  variant  calling)  

▲  CONTROL  node  (workflow  management  &  real-­‐'me  monitoring)  

▲  GAR  toolkit  (incl.  SAM/BAM/FASTQ  converter  and  IGV  viewer)    

Product  features:  ▲  Storage  on  board  (incl.  SSDs)  

▲  Embedded  Oracle  database    

▲  Graphical  User  Interface  

▲  High  level  security  

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Scalable  soluIon  

* HGE37 = Human Genome Equivalents with an average of 37x coverage sequenced # Each MAP server only contains a dual standard INTEL XEON Processor E5-2620

2 4

XS   S   M   L   XL  Storage   Maximum  number  of  HGE37*           500   1,000   2,500   4,000   10,000  

Performance    align  &  call  HGE37/day       average     48   144   240   336   624  

minutes  to  align  &  call  a  HGE37       average     30   10   6   4   2  

Average  total  realignment  days  for  fully  loaded  appliance     10   7   10   12   16  

THE  ENVIRONMENT  

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CompeIIve  environment  MAP  A  crowded  &  scagered  market  space…  

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▲  Open  source  tools  

Software Hardware

Open Source

Commercial

Complementary

▲  Commercial  specific  hardware  soluIons  

▲  Solware  soluIons/suites  ▲  Consultancy  services  ▲  Turnkey  appliances  

▲  Solware  suites  included  in  NGS  machines  by  its  vendors  

Cloud enabled

FPGA accelerated

▲  Cloud  services  ▲  Cloud  placorm  

▲  Descrip'on:  Processing  of  raw  NGS  data  in  prepara'on  of  analysis  

Market  Environment  

CompeIIve  environment  MAP  …with  many  players  

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Software Hardware

Open Source

Commercial

Complementary

Cloud enabled

FPGA accelerated

BowTie2, BWA

Market  Environment  

CompeIIve  environment  MAP  …but  also  one  with  significant  room  for  improvement  

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Speed  

Format  (C/E)  Accuracy  

Market  Environment  

THE  ROUTE  

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Business  Strategy  

▲  Key  /  Social  Opinion  Leader  management  

▲  Focus  on  high  volume  centers/ini'a'ves  

▲  Focus  high  complexity  data  opportuni'es  

▲  Disrup've  market  penetra'on  (costly)  

▲  Partnerships  with  global  top  ins'tutes  

▲  Partnerships  with  key  market  players  (e.g.  Intel,  Illumina)  

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THE  PLAN  

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Global  Launch  

42andMoore  –  seong  new  unofficial  WR  

THE  HURDLES  

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THE  HURDLES  

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▲  Team  

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The  team  

THE  HURDLES  

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▲  Team  

▲  Money  

THE  HURDLES  

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▲  Team  

▲  Money  

▲  Market  dynamics  

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The  market  

THE  HURDLES  

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▲  Team  

▲  Money  

▲  Market  dynamics  

▲  Support  

Excellent  team  and  network  Our  board  of  directors  

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Former  posiIons:  CEO  of  Crucell  (sold  to  J&J  for  2.4B)    Board  member  Galapagos  (listed  AMEX)  

Professor  of  entrepreneurship  at    IESE  Business  School  in  Barcelona    Former  posiIons:  Group  Management  Royal  Philips    Chairman  of  the  Board  at  Crucell  

Serial  Enterpreneur  Former  posiIons:  Founder  and  Managing  Director    Tango    and  RouteMobiel  

Excellent  team  and  network  Our  scien'fic  advisory  board  

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Prof.  Edwin  Cuppen  UMC  Utrecht  

Head  of  Research  Medical  Gene'cs  

 

Amsterdam  

Prof.  Gerrit  Meijer  VUmc  Amsterdam  

Principal  Inves'gator  TraIT  (CTMM)  

 

THE  HURDLES  

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▲  Team  

▲  Money  

▲  Market  dynamics  

▲  Support  

▲  Credibility  

Credibility  

▲  Live  up  to  claims  &  promises  

▲  KOL  support  

▲  Give  opportunity  to  test  drive  

▲  Push  new  format  

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                                                                           Low  cost    

                               High  speed    

High  quality    

the  

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company