YMC GC Vol12 12 · 2016. 9. 12. · Preparative device designed for both High-/Low- pressure...

Preparative Systems

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Lineup of preparative device ---------- 160, 161

Guide for selection devices and columns -- 162

BioStream----------------------------------------- 163

YMC Pilot columns ----------------------------- 164

ECO PLUS---------------------------------------- 165

LC-Forte/R ---------------------------------- 166, 167

K-Prep LAB--------------------------------------- 168

K-Prep FC ---------------------------------------- 168

K-Prep EX----------------------------------------- 169

K series-------------------------------------------- 169

DAD/DAU series ------------------------- 170~172

SB columns --------------------------------------- 173

Software -------------------------------------------- 174

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Lineup of preparative device

NameBiochromatography Devices

Multiple PreparativeHPLC Device

BioStream LC-Forte/R

Model BSTP-800BSTP-03KBSTS-03K

BSTS-10K BSTS-30K LC-Forte/R

Appearance

Flow rate range (mL/min)

1 - 800 1 - 3000 1 - 10000 1 - 30000 0.1 - 50.0

Device pressure limit (MPa)

0.5 (Max. 0.6) 30

Dimension (W X D X H mm)

800 X 900 X 1360 900 X 1100 X 1800 1200 X 1200 X 1800 2000 X 1500 X 1800 500 X 500 X 400

Control software BioStream SoftwareLC-Forte/R Software

Forte SeparaⅡ (Optional)

GMP/CSV compliance

Yes No

Explosion proof type

No No

FeatureSanitary design

3 wavelengths measurable at a time

Recycle function3 wavelengths

measurable at a time

See page 163 166, 167

NameBiochromatography Columns

Dynamic Axial Compression Columns

YMC Pilot Columns

ModelPI100/500PI100/850

PI140/500PI140/850

PI200/500PI200/850

PI300/500PI300/850

DAD-50-850SDAU-50-700S

DAD-100-850DAU-100-700S

Appearance

Inner diameter (φ , mm)

100 140 200 300 50, 100

Column length (mm)

500, 850 500, 850 850, 700

Pressure limit (MPa)

1.0 0.7 0.5 0.3 10

See page 164, 165 170 - 172

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Preparative HPLC Devices Explosion Proof Preparative HPLC Devices HPLC Pumps

K-Prep LAB K-Prep FC K-Prep EX K Series

K-Prep LAB100SK-Prep LAB100G

K-Prep LAB300SK-Prep LAB300G

K-Prep FC750SK-Prep FC750G

K-Prep FC1500SK-Prep FC1500G

K-Prep EX03KSK-Prep EX03KG




K-100 K-300 K-500 K-1000

1 - 100 1 - 300 1 - 750 1 - 1500 30 - 3000 60 - 6000 125 - 12500 250 - 25000 1 - 100 1 - 300 1 - 500 1 - 1000

15 10 10 10 10 15 10 10 10

800 X 600 X 650 700 X 750 X 1250 1100 X 1100 X 1300 Please contact us. 225 X 485 X 205 380 X 550 X 220

K-Prep Software LCD panel, Microcomputer control

Yes Yes Yes Yes Yes No

No No No Yes Yes No

Equipped with injectorsfor preparative separation

Equipped with HPLC pumpsto load samples

Custom made devices are available upon request. Standalone operation

168 168 169 169


Self-PackedAxially CompressiblePreparative Columns

SB Columns

DAD-150-850DAU-150-700

DAD-200-850DAU-200-700

DAD-300-850DAU-300-700

DAD-450-850DAU-450-700

DAD-600-850DAU-600-700

Please seepage 173.

150 - 600 (800 and 1000 are available upon request.) 50, 70, 100, 150, 200

850, 700 250, 500, 1000

10 7 10 - 2

170 -172 173

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Guide for selection devices and columns

Target component

Low molecular weightorganic compounds

Pharmaceutical products

Agricultural chemicals

Food additives

Natural products

Optical isomers

Biomolecules

Proteins

Peptides

Nucleic acids

Synthetic high-polymer

Macromolecule polymer

Separationmode

Reversed-phase

Normal-phase

Reversed-phase

Normal-phase

Reversed-phase

Normal-phase

Size exclusion

Ion exchange

Size exclusion

Size exclusion

Hydrophobic interaction

Affinity

Recommended columnRecommendedchromatography device

LC-Forte/R YMC-DispoPackAT P.126

YMC-GPC P.124, 125

Recommended column size :φ10 - φ30 mm

P.166,167

P.166,167

P.168

P.168

P.169

P.163

P.168

P.168

P.169

P.166,167

P.166,167

ECO PLUS P.165

YMC-Actus Series P.116~120

Preparative Columns P.121~

Recommended column size :φ10 - φ30 mmECO PLUS P.165



Recommended column size :φ50 - φ150 mmDAD/DAU Series P.170~172


Recommended column size : more thanφ100 mmDAD/DAU Series P.170~172

Recommended column size :φ50 - φ150 mm DAD/DAU Series P.170~172


Recommended column size : more thanφ100 mm DAD/DAU Series P.170~172

YMC Pilot Columns P.164


ECO PLUS P.165




ECO PLUS P.165 YMC-Actus Series P.116~120


LC-Forte/R

K-Prep LAB

K-Prep FC

K-Prep EX

LC-Forte/R

LC-Forte/R

K-Prep LAB

K-Prep FC

K-Prep EX

BioStream

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Specifications

Model BSTP-800 BSTP-03K BSTS-03K BSTS-10K BSTS-30K

Max. flow rate (mL/min) 800 3000 3000 10000 30000

Device pressure limit (MPa) 0.5 (Max. 0.6)

Ambient temperature (°C) 5 - 30

Wetted material PFA, PTFE, Quartz, Glass, EPDM SUS316L, PTFE, Quartz, Glass, EPDM

Sensor pH sensor, Conductivity sensor, Pressure sensor, Flowmeter sensor, UV sensor (3 variable-wavelengths measurable)

Other function Air trap, Air sensor, Column bypass and Column switching

Control software BioStream Software

Dimension (W X D X H mm) 800 X 900 X 1360 900 X 1100 X 1800 900 X 1100 X 1800 1200 X 1200 X 1800 2000 X 1500 X 1800

Weight (kg) 200 250 300 400 600

UtilitySingle –phase 100 V (15 A) Three-phase 200 V (20 A) Three-phase 200 V (30 A) Three-phase 200 V (40 A)

Instrument air, Dry air

BioStream is a biochromatography device and has been developed with YMC’s experience and technology. This system is superior

in operability, performance and quality and it achieves hygiene, speed and high purification which are required by the separation and

purification of biopharmaceuticals.

Biochromatography Devices

BioStream

FeaturesSuitable for downstream processing for biopharmaceutical manufacturing

Compliance with cGMP

Sanitary design superior in cleaning

Excellent operability provided by the largest 21.5-inch touch panel screen in this industry

Low flow pumping provided by the quintuplex diaphragm pump *

Compliance with IQ/OQ validation and CSV

* The pump for BSTP-800 is a triple diaphragm pump.

Advanced flow control

Flow diagram

BioStream is equipped with quintuplex diaphragm pumps and can pump at a low flow rate. The pumps are composed of oil-free materials. It is safe

to use BioStream at production lines. A high-accuracy servo motor and a flowmeter feedback system are combined to achieve extremely high flow-

rate precision.

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YMC Pilot columns are biochromatography columns designed for use in pilot and production scale. All wetted parts are made of

nonmetals. Column design and care in construction avoids causing extra dead volumes and serves to allow the column to be cleaned

easily. YMC Pilot columns are available in AB type, which is suitable for applications with aqueous buffer and in SR type, which are

designed for applications requiring solvent resistant. YMC Pilot columns are widely used from standard normal-phase/reversed-phase

chromatography to use with resins designed for biochromatography.

Biochromatography Columns

YMC Pilot columns

Features�Biocompatible and ideal for use in purification of

biopharmaceuticals such as proteins and peptides, etc.

�Unique frit design enables reduced losses in diffusion and uniform

performance

�Easy scale-up, having the same structure and operability across

different column sizes

Packing bed height easily adjustable by hand wheeled adjusters

Compliance with IQ/OQ validation and FDA regulations

Various options available

Specifications

Model PI100/500 PI100/850 PI140/500 PI140/850 PI200/500 PI200/850

Inner diameter (mm) 100 100 140 140 200 200

Packing bed height (mm) 50 - 430 400 - 780 55 - 420 405 - 770 70 - 435 420 - 785

Volume (L)min 0.39 3.14 0.85 6.23 2.20 13.2

max 3.38 6.13 6.47 11.9 13.7 24.7

Cross-section (cm2) 78.5 78.5 154 154 314 314

Pressure limit (MPa) 1.0 1.0 0.7 0.7 0.5 0.5

Other sizes (more than 300 mmI.D.) are available upon request.

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ECO PLUS glass columns are multi-purpose columns for almost all types of liquid chromatography applications (pressure limit 2.5 to 8.0

MPa). They are available in two forms; AB (Aqueous Buffer) versions for aqueous buffer and cold room applications and SR (Solvent

Resistant) versions for separations involving organic solvents. With a choice of adjustable length plungers at each ends and a totally

inert triple chevron sealing system the SR versions support the complete spectrum of applications from classical normal phase and

reversed phase chromatography through to biochromatography. The “Quick-Lock” connection system makes it possible to open and

seal the column simply and quickly.

Glass Columns

ECO PLUS

Features�Biocompatible

�Universal application

�Solvent resistant version

�Low temperature version available with polyethylene plunger and EPDM sealing ring

�Height adjustable plunger at both ends

�Easy to use

�Compatible with any LC system

Specifications

Model TAC05 TAC10 TAC15 TAC25 TAC35 TAC50

Inner diameter (mm) 5 10 15 25 35 50

Pressure limit (MPa)AB 8.0 8.0 7.0 5.0 4.0 3.0

SR 8.0 5.0 5.0 5.0 4.0 2.5

Column length (mm) 125, 250, 500

Usable temperature range(°C)

AB 4 - 40

SR 16 - 40

Connection1/4" - 28G fittings

(1/16" tubing)1/4" - 28G fittings

(1/8" tubing)

OptionalAB type / SR type

short plunger, short/long plunger, long plunger

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LC-Forte/R, a next-generation device, is designed for both high-/low-pressure chromatography. Key features include recycling mode

and automatic programming. LC-Forte/R is equipped with 3-wavelength UV detector, and ideal for purification of chiral compounds,

functional molecules, and natural products.

Multiple Preparative HPLC Device

LC-Forte/R

Features�Preparative device designed for both High-/Low- pressure chromatography

Ideal for purification in the crude stage through to the final stage

�3 variable-wavelengths UV detector available as a standard feature

�User friendly operation provided by touch input on graphic screen

�Compact design but equipped with multiple functions such as recycling

function, automatic programming function, etc.

�Easy maintenance

Specification

Model LC-Forte/R

Flow rate range (mL/min) 0.1 - 50.0

Device pressure limit (MPa) 30

Dimension (W X D X H mm) 500 X 500 X 400

Control software LC-Forte/R Software

Optional

Available detectors (UV+RI, RI, etc.)

Fraction collector

Software for PC

Inside the device Tubing connections

Max. flow rate

50mL/min

Max. flow rate

50mL/min

Devicepressure limit

30MPa


30MPa

3 variable-wavelengths200-400 nm

3 variable-wavelengths200-400 nm

Low-pressuregradient

Low-pressuregradient

RecyclingRecycling

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Application for recycling chromatographic separation

LC-Forte/R is equipped with recycling function. By using recycling chromatography separation method, higher resolution can be achieved on a sample

that is hardly separated, even without changing mobile phase conditions or columns. Furthermore, no solvent is consumed during recycling. It greatly

contributes to reduction of solvent consumption on purification.

0

100

200

300

400

500

mAU

0 20 40 60 min

1st

2nd

3rd4th

5th

G130904B

Column : YMC-GPC T4000 (10 μm, 10 nm) 600 X 20 mmI.D.

Eluent : chloroformFlow rate : 10 mL/minDetection : UV at 254 nmSample : polystyrene oligomers (50 mg/mL)Injection : 1.4 mLSystem : LC-Forte/R

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K-Prep LAB is an easy to use preparative HPLC member of the K-Prep series prep HPLC’s developed to achieve seamless scale-up.

K-Prep LAB is an easy to learn tabletop preparative system designed for usage at laboratory scale.

Preparative HPLC Devices

K-Prep LAB

Features�Automatic preparative purification device usable at laboratory scale provides

strong support for exploratory research.

�All-in-one device equipped with preparative injectors and fraction collectors

�Fully-automatic operation by PC

�Easy maintenance provided by superior internal structure

�Seamless scaling-up to industrial scale

Max. flow rate

100mL/min

Max. flow rate

mL/min100Devicepressure limit

15MPa


15MPa

UV/VIS195-600

nm

UV/VIS195-600

nm

Gradientmodeselectable


K-Prep LAB100

Max. flow rate

300mL/min

Max. flow rate


10MPa


10MPa

UV/VIS195-600

nm

UV/VIS195-600

nm



K-Prep LAB300

Specification

Mode LAB100S LAB100G LAB300S LAB300G

Gradient No Yes No Yes

Flow rate range (mL/min) 1 - 100 1 - 300

Device pressure limit (MPa) 15 10

Fraction collector 20-channel drip method or 5-channel switching valve method

Sample injection Preparative autoinjector

Detector UV/VIS (195 - 600 nm)

Control / Display Notebook computer / Programmable logic controller

Dimension (W X D X H mm) 800 X 600 X 650

Control software K-Prep Software

K-Prep FC is a preparative HPLC device that allows seamless scale-up from small scale production up to industrial scaling-up

processes.

Preparative HPLC Devices

K-Prep FC

Features�Smooth transition from laboratory scale to industrial scale

�Usable for production purpose

�All-in-one device equipped with HPLC pumps to load samples and fraction collectors

�Strong support for preparative purifications provided by fully-automatic operation by PC

�Compliance with IQ/OQ validation and CSV

Max. flow rate

750mL/min

Max. flow rate


10MPa


10MPa

UV/VIS195-600

nm

UV/VIS195-600

nm



CSVcomplianceCSVcompliance

K-Prep FC750

Max. flow rate

1500mL/min

Max. flow rate


10MPa


10MPa

UV/VIS195-600

nm

UV/VIS195-600

nm




K-Prep FC1500

Specification

Model FC750S FC750G FC1500S FC1500G

Gradient No Yes No Yes

Flow rate range (mL/min) 1 - 750 1 - 1500

Device pressure limit (MPa) 10

Fraction collector 5-channel switching valve method

Sample injection HPLC pump to load samples (1 - 300 mL/min)

Detector UV/VIS (195 - 600 nm)

Control / Display Notebook computer / Programmable logic controller

Control software K-Prep Software

Utility AC100V, 0.5 MPa dry air

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K-Prep EX is an explosion proof HPLC preparative device designed for GMP manufacturing plants.

Explosion Proof Preparative HPLC Devices

K-Prep EX

K series pumps are designed for preparative chromatography employed worldwide for simple and demanding applications. K-Prep

dual reciprocating plungers and the high precision servo motor enable highly-accurate pumping with less pulsation.

HPLC Pumps

K series

Features�A wide range of flow rates and range allow a minimum flow rate 1/1000 of the maximum.

�Usable as a high pressure mixing gradient system by adding on a sub-pump

�Gradient and flow rate programmable provided by microcomputer control

�Max. pressure limit can be set enabling column overpressure protection.

�Plunger wash ports (optional) to prevent salt deposition and premature seal failure

�Explosion proof pumps (optional) available

Features�Explosion proof device

�This explosion proof HPLC unit may be installed in a hazardous area

and controlled from a safe area

�Operable at hazardous area by operation station (optional)

�Custom-made device available upon request

�Compliance with IQ/OQ validation and CSV

�Documentation maintenance feature allows for adjusting recordkeeping

to meet your needs

Explosionproof

Explosionproof

UV/VIS195-600

nm

UV/VIS195-600

nm




Specification

Model K-100 K-300 K-500 K-1000

Flow rate range (mL/min) 1 - 100 1 - 300 1 - 500 1 - 1000

Device pressure limit (MPa) 15 10 10 5

Pumping mechanism Dual plunger linear cam driven

Pumping method Constant flow

Wetted material SUS316, Zirconia, Reinforced Teflon, PEEK

Dimension (W X D X H mm) 225 X 485 X 205 380 X 550 X 220

Flow diagram

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The DAD series are an automatic self-packing type of dynamic axial compression column that allows for an easy, cost-effective

refill of packing material that results in a column bed yielding superior durability and reproducibility. The DAD series are available in

columns for laboratory, pilot, and industrial scale.


DAD series: Driving direction of cylinder is downward

Features�Suitable for high purification in various fields such as pharmaceuticals, fine chemicals and functional foods

�Cost-effective self-packing dynamic axial compression columns

�Optional slurry container permits automated packing procedures.

�Superior column performance, durability and reproducibility provided by usage at constant pressure

�Easy unpacking operation with downward cylinder driving direction

�No need to prepare lifting equipment after installation

�Compliance with IQ/OQ validation

Specification

Model DAD-50 DAD-100 DAD-150 DAD-200 DAD-300 DAD-450 DAD-600 DAD-800 DAD-1000

Inner diameter (φ, mm) 50 100 150 200 300 450 600 800 1000

Pressure limit (MPa) 10 7

Column length (mm) 850

Column bed length (mm) 100 - 500

Slurry container volume (L)* 2 10 22 30 80 160 300 600 1200

* Slurry containers are optional.

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The DAU series are an automatic self-packing type of dynamic axial compression column that allows for an easy, cost-effective

refill of packing material that results in a column bed yielding superior durability and reproducibility. DAU columns have been selling

more than 10 years and have a good reputation of maintaining stable performance over years after installation. The DAU series are

available in columns for laboratory, pilot, and industrial scale.


DAU series: Driving direction of cylinder is upward

Features�Suitable for high purification in various fields such as pharmaceuticals, fine chemicals and functional foods

�Cost-effective self-packing dynamic axial compression columns

�Optional slurry container permits automated packing procedures

�Superior column performance, durability and reproducibility provided by usage at constant pressure

�Compact and sophisticated design based on our abundant experience

�Compliance with IQ/OQ validation

Specification

Model DAU-50 DAU-100 DAU-150 DAU-200 DAU-300 DAU-450 DAU-600

Inner diameter (φ, mm) 50 100 150 200 300 450 600

Pressure limit (MPa) 10 7

Column length (mm) 700

Column bed length (mm) 100 - 400

Slurry container volume (L)* 2 10 22 30 60 140 250

* Slurry containers are optional.

Slurry container

The slurry container provides excellent

distribution of packing material and allows for a

“homogeneous” slurry that can be automatically

loaded into the DAU/DAD column by simply

switching a valve. Throughout the operation

from slurry preparation to filling slurry, safety and

hygiene can be achieved.

P

Circulation

Column

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Packing procedure

Column performance

The data on the right hand side shows repacking data obtained by

using a 50 mmI.D. DAU column(DAU-50). After 7 times repacking,

plates/m and Tf are still as good as the initial state.

plat

es/m

Tf

Change in chromatogram Change in column performance

Seamless scale-up

The chromatograms on the right hand side are a scale-up example

from an analytical 4.6 mmI.D. column to a 50 mmI.D. DAU column.

This indicates that the very similar separation pattern of analytical

scale is also reproducible at the preparative scale.

analytical

Scale up

preparative

Column : YMC*GEL C8 (S-10 μm, 20 nm) 150 X 50 mmI.D.

Eluent : A) water/TFA (100/0.1) B) acetonitrile/TFA (100/0.1) 25-60%B (0-20min)

Flow rate : 100 mL/minTemperature : 37°CDetection : UV at 220 nm

Column : YMC-Pack C8 (S-5 μm, 20 nm) 150 X 4.6 mmI.D.

Flow rate : 0.8 mL/min

1.Cytchrome c MW 124002.Insulin MW 57003.Lysozyme MW 144004.α-Lactalbumin MW 142005.Myoglobin MW 17000


Ease of use and outstanding column performance in a single system

Fill slurrySlurry solventevacuation

Compression Completion of a column

Slurrysupport

Fill packing materialthrough slurry pump.

Perform chromatography.Compress the columnand push out extra slurrysolvent.

Slurry

Support

Packing/compression Operation

DAU Series : Refer to the drawings.

DAD Series : Compressive direction is reversed.

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SB products are statically compressed cost effective preparative columns which enable convenient self-packing. Tightening screw

bolts of the upper flange yields a compressed packing bed. Not only dry packing but also wet (slurry) packing is possible by attaching

a column packing extender (optional).

Self-Packed Axially Compressible Preparative Columns

SB columns

Features�Superior column performance and reproducibility provided by high density packing

Maintains great durability utilizing manual pressurization using screw bolts

Eliminate voids (occurred by long-term usage) by re-tightening screw bolts

Cost effective provided by self-repacking

Packing service (optional) available with YMC’s preparative packing materials

Column packing extender (optional) and column stand (optional) available

Specification

Model SB-50 SB-70 SB-100 SB-150 SB-200

Inner diameter (φ, mm) 50 70 100 150 200

Pressure limit (MPa) 10 7 7 5 5

Column length (mm) 250 / 500 / 1000

Column bed length (mm) 200 - 300 / 450 - 550 / 900 - 1100

OptionalColumn packing extender

Column stand

Please contact us for SB Columns with 300 mmI.D. or larger.

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Software allowing seamless scale-up from laboratory scale to process scale

User friendly, flexible and intuitive programming and operation

Compliance with FDA 21 CFR Part 11, cGMP and CSV

Software (for Chromatography Devices)

K-Prep, BioStream

Liquid crystal touch panel allows comfortable visual touch.

Standard features include software for displaying chromatograms on the touchscreen.

Various functions are available such as recycling, sample injection (stacking), and automated cleaning.

�When operated in conjunction with optional external PC control software quantitative (area%) and GPC measurement may be

employed.

Software (for Chromatography Devices)

LC-Forte/R

CSVCSVcGMPcGMPFDA21 CFR Part 11

FDA21 CFR Part 11

Software for K-Prep and BioStream are the

latest chromatography device control software.

The software has been developed to be intuitive

based on the operator’s reaction to visual

operation. The software enables complicated

preparative purifications to be performed easily

and automatically.

The dedicated software for LC-Forte/R has been developed to put the full power of the instrument within your grasp.

YMC GC Vol12 12 · 2016. 9. 12. · Preparative device designed for both High-/Low- pressure...

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Transcript of YMC GC Vol12 12 · 2016. 9. 12. · Preparative device designed for both High-/Low- pressure...