WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two...
Transcript of WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two...
![Page 1: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/1.jpg)
Roberto Soares, DVM, MAM, ACPV Regional Technical Manager - Poultry
Ceva Animal Health – APAC
Malaysia
WPSA & WVPA
Scientific Conference 2013
![Page 2: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/2.jpg)
Outlook of the presentation
Major changes in the Asia Poultry Industry
Fast growth of poultry industry
Disease Challenge
Role of New technology vaccine for Newcastle
Disease control
Vector vaccines
Evaluation of Efficacy
Compatibility
Conclusions
![Page 3: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/3.jpg)
Source: National geographic archive
Population growth
![Page 4: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/4.jpg)
Source: National geographic archive
By 2050
> 9 billion people
will be living on Earth
![Page 7: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/7.jpg)
7
Main Source of
Protein
![Page 8: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/8.jpg)
2011
8
80.8 (million tones) E 140 (million tones)
2050
![Page 9: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/9.jpg)
Growth of broiler meat production (1,000 MT)
Country 2003 2012 Growth (%)
China 9,898 13,700 + 38.4 India 1,500 3,160 + 110.7 Thailand 1,340 1,550 + 15.7 Indonesia 1,118 1,540 + 37.7 Malaysia 835 955 + 14.4 Philippines 635 790 + 24.4 Vietnam 373 750 + 101.1
Total 15,699 22,445 + 43.0
Source: USDA; http://www.indexmundi.com
![Page 10: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/10.jpg)
Changes in the Asia Poultry Industry
Larger production units
High stocking density
Increased farm density
Marginally efficient respiratory system
Complicated automation equipment
Farms with different biosecurity standards closely located
Reduced downtime period
Poorly qualified labour
Pressure to reduce the use of anti-infectives
Strong disease
pressure
![Page 11: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/11.jpg)
12
Disease Challenge
![Page 12: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/12.jpg)
Newcastle Disease Challenge
13
![Page 13: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/13.jpg)
14
![Page 14: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/14.jpg)
rHVT-ND
![Page 15: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/15.jpg)
Recombinant ND vaccines
NDV
Donor = NDV
NDV Genome
F gene
F protein
HVT
Vector = HVT virus
Insertion site (nonessential sites on
HVT genome)
rHVT - ND
F protein
F = Fusion
![Page 16: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/16.jpg)
How to evaluate a vaccine?
17
Level of
protection
Onset
of
immunity
Type of
immune
response
Duration
of
immunity
Spectrum
of
immunity
Reduce
re-excretion
Ability of
evade MDA
Compatibility
![Page 17: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/17.jpg)
How to evaluate a vaccine?
18
Level of
protection
Onset
of
immunity
Type of
immune
response
Duration
of
immunity
Spectrum
of
immunity
Reduce
re-excretion
Ability of
evade MDA
Compatibility
![Page 18: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/18.jpg)
Active Immune Response
HI test is the most sensitive and specific method for the reliable detection
of the immune response induced by rHVT-NDV vaccine.
Source: Palya et al. Monitoring the vaccination with a rHVT-NDV vectored vaccine in commercial broilers through serological
methods. Poster at AAAP Meeting, San Diego – CA, 2012.
![Page 19: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/19.jpg)
20
NDV specific cell mediated response
Active Immune Response
Cell-mediated immunity.
![Page 20: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/20.jpg)
How to evaluate a vaccine?
21
Level of
protection
Onset
of
immunity
Type of
immune
response
Duration
of
immunity
Spectrum
of
immunity
Reduce
re-excretion
Ability of
evade MDA
Compatibility
![Page 21: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/21.jpg)
Onset of immunity
Source: Extrated from Palya et al. Compatibility of Vectormune® ND with Cevac® Transmune in day-old commercial broilers.
SIS # 02/2012/P/SSIU CP, October 26, 2012.
0
20
40
60
80
100
W 2 W 3 W 4 W 6
% p
rote
cti
on
Age of challenge
Protection against vvNDV challenge (Clinical protection)
rHVT-NDV Control
Broiler day-old chicks vaccinated with one full dose of rHVT-NDV vaccine.
NDV challenges: Malaysian isolate ref. D1524/1/1,2/MY/10, viscerotropic velogenic NDV
strain, genotype VII, 5 log10 EID50/bird, nasal route (200 µl).
![Page 22: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/22.jpg)
Onset of immunity
Source: Satra et al. Efficacy of several vaccination programs against ND challenge. - WVPA Congress, Cancun , 2011.
0
20
40
60
80
100
rHVT-NDV + live ND vaccine Control
% p
rote
cti
on
Protection after ND challenge
Week 2
Week 3
Week 4
Layer day-old chicks vaccinated with one full dose of rHVT-NDV & live ND vaccines.
NDV challenges done at 14, 21 and 28 days of age with a velogenic ND virus (Thai Lopburi
strain – ICPI 1.86) at a dosage of 105 EID50 per bird by intramuscular injection.
In high ND challenge areas, it is recommended to associate the rHVT-NDV with a live ND
vaccine at day of age. In some cases, a booster in the farms should be added.
![Page 23: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/23.jpg)
Broiler day-old chicks were vaccinated subcutaneously with
comparable doses of the two rHVT-ND vaccines.
A recent velogenic NDV isolate belonging to genotype VII
(D1524/1/1,2/MY/10) was used for challenges.
Clinical protection against NDV challenge was tested
weekly from 3 to 6 weeks of age.
rHVT-NDV vs rHVT-NDV
Source: Palya et al. Markedly different onset of protection elicited by two HVT-based recombinat ND vaccines. Proceedings of the
WVPA Congress - Nantes, p. 448, August 2013.
![Page 24: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/24.jpg)
Clinical protection
Results
Source: Palya et al. Markedly different onset of protection elicited by two HVT-based recombinat ND vaccines. Proceedings of the
WVPA Congress - Nantes, p. 448, August 2013.
0
20
40
60
80
100
W 3 W 4 W 5 W 6
% p
rote
cti
on
Age of challenge
Protection against vvNDV challenge
rHVT-NDV 1 rHVT-NDV 2 Control
![Page 25: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/25.jpg)
How to evaluate a vaccine?
26
Level of
protection
Onset
of
immunity
Type of
immune
response
Duration
of
immunity
Spectrum
of
immunity
Reduce
re-excretion
Ability of
evade MDA
Compatibility
![Page 26: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/26.jpg)
Material & Methods
Commercial laying pullets.
0 …. 3 4 … 6 ….. 10 ….. 15 ….….. 25 …..… 33 ….. 40 …..…. 55 …………. 72
Weeks of age
rHVT-NDV
rHVT-NDV +
Live ND vacc ND Killed
Group 1
Group 2
Groups 4 Negative & Positive Control groups - No vaccination
rHVT-NDV +
Live ND vacc ND Killed Groups 3
Monitoring parameters (during 14 days):
Daily observations for NDV specific clinical signs, mortality and egg production (33 wk
challenge)
LaSota
* Effect of NDV challenge on the egg production
*
Challenge with a Genotype VII strain (Malaysian isolate) 5.0 log10 EID50/bird by intranasal route (0.1 ml)
![Page 27: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/27.jpg)
NB: VTM-ND & Vitapest & LaSota & killed group was challenged only 4 weeks after LaSota booster (before vaccination with the
killed ND vaccine) and at 55 wks of age.
Clinical protection
Under the conditions of this trial, the rHVT-NDV vaccine alone induced a
strong immunity (clinical signs + shedding controls) up to 72 weeks of age.
![Page 28: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/28.jpg)
How to evaluate a vaccine?
29
Level of
protection
Onset
of
immunity
Type of
immune
response
Duration
of
immunity
Spectrum
of
immunity
Reduce
re-excretion
Ability of
evade MDA
Compatibility
![Page 29: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/29.jpg)
Commercial broilers (20 per group)
rHVT-NDV SQ or in-ovo (-3d)
Challenge :
105 EID50 Chimalhuacan NDV strain (Genotype V)
at 3 or 4 or 6 weeks of age
IN + ON route
Oropharyngeal and Cloacal swabs taken
3 and 7 days post challenge
Virus quantification using RT-PCR
Materials & Methods
![Page 30: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/30.jpg)
Clinical protection
Results
![Page 31: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/31.jpg)
Reduction of shedding
Results
* * * * * * * *
Challenge at D27
![Page 32: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/32.jpg)
Reduction of shedding
Results
Challenge at D40
* * * * * * * *
![Page 33: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/33.jpg)
How to evaluate a vaccine?
35
Level of
protection
Onset
of
immunity
Type of
immune
response
Duration
of
immunity
Spectrum
of
immunity
Reduce
re-excretion
Ability of
evade MDA
Compatibility
![Page 34: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/34.jpg)
Spectrum of immunity
Does it protect
against
various types
of NDV ?
F
insert Yes !
Yes !
Yes !
Yes !
![Page 35: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/35.jpg)
How to evaluate a vaccine?
37
Level of
protection
Onset
of
immunity
Type of
immune
response
Duration
of
immunity
Spectrum
of
immunity
Reduce
re-excretion
Ability of
evade MDA
Compatibility
![Page 36: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/36.jpg)
Compatibility
Rispens rHVT-ND
![Page 37: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/37.jpg)
39
![Page 38: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/38.jpg)
Compatibility studies
HVT rHVT-ND
![Page 39: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/39.jpg)
![Page 40: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/40.jpg)
42
Conclusions
The poultry industry has developed remarkably in the recent
years.
Genetic improvement, nutrition, husbandry, housing systems,
animal health etc.
New technology vaccine plays an important role in the
control of Newcastle Disease
Safe
Prevent clinical signs
Reduces shedding
Induces strong and persistent immunity
![Page 41: WPSA & WVPA Scientific Conference 2013 · strain, genotype VII, ... comparable doses of the two rHVT-ND vaccines. ... The poultry industry has developed remarkably in the recent](https://reader031.fdocuments.net/reader031/viewer/2022030800/5b09ded27f8b9a45518b50a3/html5/thumbnails/41.jpg)