Weaning mice. Remove weaning-age mice (usually 21 days) from the parent cage. Separate the pups by...
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Transcript of Weaning mice. Remove weaning-age mice (usually 21 days) from the parent cage. Separate the pups by...
Weaning mice• Remove weaning-age mice
(usually 21 days) from the parent cage.
• Separate the pups by sex.• Mark the parent cage with a
separation card; mark the weaned cages with separation stickers
Before you startCheck the problem log to see if you
expect to have any separations
Check the room for green OC cards
Before you start• Check your watch!!
What time is it?
• If you don’t have a watch, check the time on your pager
Why are you checking the time?
So you can fill out the Special Services Log when you are done.
Date PrincipalInvestigator’s Name
Types of ServiceCage Separation (# of cages)
Types ofServiceOther Services
Number of Hours
Performed By (Print Name)
Initials
Before you start• Double-check the
OC card– Is it a supervisor
check?– Double-check that
the weaning due date is correct- look at the weaning date calendar.
Weaning mice• Remove weaning-age mice (usually
21 days) from the parent cage.
• Separate the pups by sex.
• Complete the necessary paperwork
For pups, the ano-genital distance is used to determine sex.
It isn’t so easy to sex It isn’t so easy to sex pupspups
Male
Female
Female
• Female pups have a shorter anogenital distance
• Female pups have nipples (which may not be visible if the pups are furry)
Sex pups 2 at a time
• If they look the same, try again until you get one that looks different.
• The one with the longer anogenital distance is the male. The other is the female.
• Keep one pup of known sex, and use it to compare against the rest of the pups.
Weaning set-up• Parent cage• Male pup cage• Female pup cage
• Even if you have more than 10 pups, start by separating the pups by sex into two cages.
Housing pups • 5 females per cage• 4 males per cage
• Recheck the sex• Put pellets of food on the cage floor• Complete the green separation cards and
stickers• House the separated cages on the separation
shelf
Separation cardsThe white sticker (with the separation
number) goes on the green card. Write the date and the number of new
cages created on the white sticker
Before you finish• Update the census• Initial the ‘addressed’ column in
the problem log• Fill out the Special Services Log.
Fill out the Special Services Log.
• Fill out the Log. Put the number of new cages created in the separation column.
• Look at your watch to determine the time to complete the separation. The whole time (including paperwork) counts.
Boston University Medical Campus Laboratory Animal Science Center
Special Services Log
Room No. Year: Date Principal
Investigator’s Name
Types of Service Cage Separation (list the number of new cages created only)
Types of Service Other Services
Number of Hours
Performed By (Print Name)
Initials
8/11 Smith 3 35 min
Shomer NS