Wang Xueling

December 24, 2010

The study for the nature antitumor products from marine

microorganisms

Main content

Research Background and Meaning

The main content of the study and the the

steps of the experiment

The experimental methods

The disease of cancer

Cancer to Become the Leading Cause of Death Worldwide:

World Health Organization (WHO)

Cancer will exceed heart disease as the leading cause of death by the end of 2010, an alarming trend that could more than double global cancer cases and deaths by 2030.

Extensive tobacco use in developing countries contributes to this crisis, particularly in China and India, where 40 percent of the world's smokers now live.

WHO reports cancer diagnoses around the world rise along with death rate: approximately 12 million in 2009 as global cancer deaths are expected to reach 7 million.

Situation of Study about the natural antitumor products derived from marine microorganisms

Some of the marine animals ,such as sponge coral and seaweed have strong antitumor activity,and studies have showed that it is the microorganisms in these animals

which have the antitumor activity,not the animals themselives

Antitumor antibiotics

the antitumor activity of

the marine life

the antitumor activity of the marine microorganics

Derived from microoganisms

the steps of the study

Fiscal Year 2006Obtain the metabolin of the bactia from fementation

Selct one of the bacteria which have the best antitumor activity through the MTT assay

Isolate kinds of compoud through Thin Layer Chromatography (TLC) and gel column chromatography from the one which have the best activity , then test the antitumor activity through MTT assay

The further study about the bacteria and its active substance,such as ,the best growing condition of this bacteria

,analysis the active substance through the gas chromatograph(GC)or the HPLC,or some others

TLC

gel column chromatography

The experimental methods(What is the What is the MTT assay?MTT assay?)

Cultured cancer cells are grown in the presence of potential Cultured cancer cells are grown in the presence of potential drug for a specific time perioddrug for a specific time period

The amount of viable cells remaining can be determined The amount of viable cells remaining can be determined spectrophotometrically spectrophotometrically

Living cells convert the yellow water soluble-tetrazolium salt Living cells convert the yellow water soluble-tetrazolium salt into an insoluble purple formazan crystalinto an insoluble purple formazan crystal

Crystal is dissolved in a suitable solvent (e.g. DMSO, acid-Crystal is dissolved in a suitable solvent (e.g. DMSO, acid-IPA) and the absorbance at 570 nm is obtainedIPA) and the absorbance at 570 nm is obtained

A dose response curve is plotted and the concentration at A dose response curve is plotted and the concentration at 50% cell growth is obtained50% cell growth is obtained

The two cancer cells in the study

The hela cell

Sm

mc 7

72

1